Click here to switch to the map view.

The map label for this gene is folC [H]

Identifier: 157961444

GI number: 157961444

Start: 1959484

End: 1960749

Strand: Direct

Name: folC [H]

Synonym: Spea_1618

Alternate gene names: 157961444

Gene position: 1959484-1960749 (Clockwise)

Preceding gene: 157961443

Following gene: 157961445

Centisome position: 37.87

GC content: 45.26

Gene sequence:

>1266_bases
ATGCCAGTAGCACCAAAGTCAGGTGCTACGTTAAACCGCTGGTTAGATTTCTTATTGGCAATTCACCCAACTGAAATTGA
TATGGGACTTGCACGGGTATCAACTGTCGCTACTAGAATGGAATTGAACCATTTAGGCGAAACCAAAATAGTCACTGTTG
CAGGCACTAATGGTAAAGGCACTACCTGCGCCATGATCGAAGCTGCGTTACAGCTATCAGGGAAAACGGTAGGCGTATAC
AGTTCACCGCATTTACTGAACTACAATGAGCGCGTGCGGATTAATGGTATTGATGCTACTGATACAGCTTTAGTCGGTGC
CTTCGAAGCCATTGAAGCTGCTCGAGCCGAGATCTCATTAACCTTCTTTGAGTACGCAACGCTAGCTGGTCTTTATCTCT
TTAAAGCTGCCAAGCTTGATGTGGTATTACTCGAGGTAGGTTTAGGTGGTCGCTTAGACGCAACCAACTTAATCGATGCA
GATATTGCAGTCGTGACTTCTATCGATCTTGACCATCAAGAGTACCTTGGTGATACGCGAGAGTTGGTTGGCGCAGAAAA
AGCAGGAATATTTAGACAAGGGCGCCCTGCAATTGTAGGTGAGCCTGATTGTCCACAATCGGTACTTGATGCGGCCAATC
GAGTCGGTGCATCGCTTTATCGAGTTAATAGTGAGTTTAGCTACCAGTGCACTAACGAGGATTGGCATTTCAAAGGTCAG
CATTTCGATTTATCGCATTTGCCACTGCCATATTTGCCTCAACCTAATGCCGCCACAGTTATTGCGGTACTAGAGCACCT
TTTTCCAACTATTTCCGCCGAAATTATTGCTAAAGCCGTCGGTAACACTCAGCTCGCTGGACGTTTAGAAACTCTTAATC
CGTCACCTAAAGTTATAGTCGATGTCGCCCATAATCCTCACGCTGCACGTTATTTAAAATCACGTTTAGCTGCGATGGTA
AAAGGGCGTTTATTTGCACTTTGTGGCATGTTAAAAGATAAAGATGCTCGCTCGGTACTACTTGAGCTATCAAGTGATGT
TGATAAGTGGTTTTTATGCGACTTATCGGTCCCACGTGGCGCATCGGCAGCAGAGTTAAACGCACTAATGCCGCAAGATG
TAGACGCTGCCACTTATCAGCAGATGAGCGATGCTTGGCAAGCGATCAAAGCGCAATTGCACAGCGATGATATCGTTATT
GTATTTGGCTCATTTTACACTGTTGCGGGTTTTAAAGAGTTGTTTAAGGGAGAGAAACTTGTCTAG

Upstream 100 bases:

>100_bases
TAAGGCATGTTTAATGAGAGTTAAGGATAATCTTAACGGTAAAGGTTTGGGCCTTTACGTTTTAAATGGATAAAAGTTTG
TGATTGAACAAGACACATTA

Downstream 100 bases:

>100_bases
TCAATTTCAAAACCGTCTTGTTGGTGTCATCGTGATTGTGGCATTAGGGGTGATTTTTTTACCCGATATATTAGATGGTA
AAAAGCAGCGAGAAGAAGAG

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MPVAPKSGATLNRWLDFLLAIHPTEIDMGLARVSTVATRMELNHLGETKIVTVAGTNGKGTTCAMIEAALQLSGKTVGVY
SSPHLLNYNERVRINGIDATDTALVGAFEAIEAARAEISLTFFEYATLAGLYLFKAAKLDVVLLEVGLGGRLDATNLIDA
DIAVVTSIDLDHQEYLGDTRELVGAEKAGIFRQGRPAIVGEPDCPQSVLDAANRVGASLYRVNSEFSYQCTNEDWHFKGQ
HFDLSHLPLPYLPQPNAATVIAVLEHLFPTISAEIIAKAVGNTQLAGRLETLNPSPKVIVDVAHNPHAARYLKSRLAAMV
KGRLFALCGMLKDKDARSVLLELSSDVDKWFLCDLSVPRGASAAELNALMPQDVDAATYQQMSDAWQAIKAQLHSDDIVI
VFGSFYTVAGFKELFKGEKLV

Sequences:

>Translated_421_residues
MPVAPKSGATLNRWLDFLLAIHPTEIDMGLARVSTVATRMELNHLGETKIVTVAGTNGKGTTCAMIEAALQLSGKTVGVY
SSPHLLNYNERVRINGIDATDTALVGAFEAIEAARAEISLTFFEYATLAGLYLFKAAKLDVVLLEVGLGGRLDATNLIDA
DIAVVTSIDLDHQEYLGDTRELVGAEKAGIFRQGRPAIVGEPDCPQSVLDAANRVGASLYRVNSEFSYQCTNEDWHFKGQ
HFDLSHLPLPYLPQPNAATVIAVLEHLFPTISAEIIAKAVGNTQLAGRLETLNPSPKVIVDVAHNPHAARYLKSRLAAMV
KGRLFALCGMLKDKDARSVLLELSSDVDKWFLCDLSVPRGASAAELNALMPQDVDAATYQQMSDAWQAIKAQLHSDDIVI
VFGSFYTVAGFKELFKGEKLV
>Mature_420_residues
PVAPKSGATLNRWLDFLLAIHPTEIDMGLARVSTVATRMELNHLGETKIVTVAGTNGKGTTCAMIEAALQLSGKTVGVYS
SPHLLNYNERVRINGIDATDTALVGAFEAIEAARAEISLTFFEYATLAGLYLFKAAKLDVVLLEVGLGGRLDATNLIDAD
IAVVTSIDLDHQEYLGDTRELVGAEKAGIFRQGRPAIVGEPDCPQSVLDAANRVGASLYRVNSEFSYQCTNEDWHFKGQH
FDLSHLPLPYLPQPNAATVIAVLEHLFPTISAEIIAKAVGNTQLAGRLETLNPSPKVIVDVAHNPHAARYLKSRLAAMVK
GRLFALCGMLKDKDARSVLLELSSDVDKWFLCDLSVPRGASAAELNALMPQDVDAATYQQMSDAWQAIKAQLHSDDIVIV
FGSFYTVAGFKELFKGEKLV

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=191, Percent_Identity=36.6492146596859, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI66932990, Length=191, Percent_Identity=36.6492146596859, Blast_Score=122, Evalue=6e-28,
Organism=Escherichia coli, GI1788654, Length=420, Percent_Identity=48.5714285714286, Blast_Score=379, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17553150, Length=159, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71984923, Length=159, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17553148, Length=159, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323760, Length=319, Percent_Identity=28.5266457680251, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324815, Length=390, Percent_Identity=26.6666666666667, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24641571, Length=192, Percent_Identity=33.3333333333333, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24581568, Length=181, Percent_Identity=30.939226519337, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 45593; Mature: 45462

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVAPKSGATLNRWLDFLLAIHPTEIDMGLARVSTVATRMELNHLGETKIVTVAGTNGKG
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
TTCAMIEAALQLSGKTVGVYSSPHLLNYNERVRINGIDATDTALVGAFEAIEAARAEISL
CHHHHHHHHHHHCCCEEEEECCCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEEE
TFFEYATLAGLYLFKAAKLDVVLLEVGLGGRLDATNLIDADIAVVTSIDLDHQEYLGDTR
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCHHHHCCHHH
ELVGAEKAGIFRQGRPAIVGEPDCPQSVLDAANRVGASLYRVNSEFSYQCTNEDWHFKGQ
HHHCCHHCCCCCCCCCCEECCCCCCHHHHHHHHHHCCEEEEECCCCEEEECCCCCEECCC
HFDLSHLPLPYLPQPNAATVIAVLEHLFPTISAEIIAKAVGNTQLAGRLETLNPSPKVIV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCEEECCCCCCCEEE
DVAHNPHAARYLKSRLAAMVKGRLFALCGMLKDKDARSVLLELSSDVDKWFLCDLSVPRG
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCCC
ASAAELNALMPQDVDAATYQQMSDAWQAIKAQLHSDDIVIVFGSFYTVAGFKELFKGEKL
CCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCC
V
C
>Mature Secondary Structure 
PVAPKSGATLNRWLDFLLAIHPTEIDMGLARVSTVATRMELNHLGETKIVTVAGTNGKG
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
TTCAMIEAALQLSGKTVGVYSSPHLLNYNERVRINGIDATDTALVGAFEAIEAARAEISL
CHHHHHHHHHHHCCCEEEEECCCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEEE
TFFEYATLAGLYLFKAAKLDVVLLEVGLGGRLDATNLIDADIAVVTSIDLDHQEYLGDTR
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCHHHHCCHHH
ELVGAEKAGIFRQGRPAIVGEPDCPQSVLDAANRVGASLYRVNSEFSYQCTNEDWHFKGQ
HHHCCHHCCCCCCCCCCEECCCCCCHHHHHHHHHHCCEEEEECCCCEEEECCCCCEECCC
HFDLSHLPLPYLPQPNAATVIAVLEHLFPTISAEIIAKAVGNTQLAGRLETLNPSPKVIV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCEEECCCCCCCEEE
DVAHNPHAARYLKSRLAAMVKGRLFALCGMLKDKDARSVLLELSSDVDKWFLCDLSVPRG
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCCC
ASAAELNALMPQDVDAATYQQMSDAWQAIKAQLHSDDIVIVFGSFYTVAGFKELFKGEKL
CCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]