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The map label for this gene is truA [H]

Identifier: 157961443

GI number: 157961443

Start: 1958601

End: 1959386

Strand: Direct

Name: truA [H]

Synonym: Spea_1617

Alternate gene names: 157961443

Gene position: 1958601-1959386 (Clockwise)

Preceding gene: 157961442

Following gene: 157961444

Centisome position: 37.85

GC content: 46.56

Gene sequence:

>786_bases
ATGCGGGTTGCGCTAGGTATAGAGTACGATGGTAGTCAATATTTCGGATGGCAACGTCAGGCGGAAGTTGACACAGTGCA
AGAGCGATTAGAAAAAGCGCTTTCTATTGTTGCCAACGAACCTATTGCAGTGCACTGTGCTGGCCGAACTGATGCGGGCG
TCCACGCGACAGGTCAAGTGGTTCACTTTGAAACCAATGCGATTAGAAAAGAGACGGCTTGGACACTAGGCGTCAATGTT
AACCTGCCTGACAATATTGCTGTGCGCTGGGTTAAAGTCGTTGATGATGAATTTCACGCACGTTTTACTGCTACGGCAAG
ACGTTATCGCTATATGATTTACAATCACCAGCTTCGTCCAGGCATTTTGCGTAGCGGTGTCAGTCATTACCCAGGTGATA
TCGACGAGTCTAAGATGCATCAAGCCGCGCAGTACCTTCTCGGTGAGCAGGATTTTACTAGTTTTAGAGCTGTTCATTGT
CAGTCTAATACGCCGTTTCGCTGCGTGCATGAAGTTAACGTGACGCGTCAAGGGATGTATATCTGTGTCGATATTAAGGC
TAATGCTTTCTTACATCACATGGTGCGCAATATTGTAGGCTCTCTGTTGGAAGTCGGTTTAGGTAATCAGTCTGTCGAGT
GGATTGAACAGCTACTGGCGCTTAAAGACAGAAATAAAGCCGCAGCAACTGCCAAGCCTAACGGCTTGTATTTAGTGGAC
GTGACTTACCCTGAAAGCTACCAATTGCCTAAGTTGTCACTTGGCCCATTATTTATGTTGGACTAA

Upstream 100 bases:

>100_bases
CTAGAATCGCCGGCTATTAAAAGATTTTGTCTATCCGACTGCATTGCCTTGAAAACACAGGCAGTCGAATAACAGTTTAA
CTGAGTTATAGAGGTGTGGA

Downstream 100 bases:

>100_bases
GGCATGTTTAATGAGAGTTAAGGATAATCTTAACGGTAAAGGTTTGGGCCTTTACGTTTTAAATGGATAAAAGTTTGTGA
TTGAACAAGACACATTAATG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MRVALGIEYDGSQYFGWQRQAEVDTVQERLEKALSIVANEPIAVHCAGRTDAGVHATGQVVHFETNAIRKETAWTLGVNV
NLPDNIAVRWVKVVDDEFHARFTATARRYRYMIYNHQLRPGILRSGVSHYPGDIDESKMHQAAQYLLGEQDFTSFRAVHC
QSNTPFRCVHEVNVTRQGMYICVDIKANAFLHHMVRNIVGSLLEVGLGNQSVEWIEQLLALKDRNKAAATAKPNGLYLVD
VTYPESYQLPKLSLGPLFMLD

Sequences:

>Translated_261_residues
MRVALGIEYDGSQYFGWQRQAEVDTVQERLEKALSIVANEPIAVHCAGRTDAGVHATGQVVHFETNAIRKETAWTLGVNV
NLPDNIAVRWVKVVDDEFHARFTATARRYRYMIYNHQLRPGILRSGVSHYPGDIDESKMHQAAQYLLGEQDFTSFRAVHC
QSNTPFRCVHEVNVTRQGMYICVDIKANAFLHHMVRNIVGSLLEVGLGNQSVEWIEQLLALKDRNKAAATAKPNGLYLVD
VTYPESYQLPKLSLGPLFMLD
>Mature_261_residues
MRVALGIEYDGSQYFGWQRQAEVDTVQERLEKALSIVANEPIAVHCAGRTDAGVHATGQVVHFETNAIRKETAWTLGVNV
NLPDNIAVRWVKVVDDEFHARFTATARRYRYMIYNHQLRPGILRSGVSHYPGDIDESKMHQAAQYLLGEQDFTSFRAVHC
QSNTPFRCVHEVNVTRQGMYICVDIKANAFLHHMVRNIVGSLLEVGLGNQSVEWIEQLLALKDRNKAAATAKPNGLYLVD
VTYPESYQLPKLSLGPLFMLD

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=267, Percent_Identity=27.7153558052434, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1788657, Length=260, Percent_Identity=70.7692307692308, Blast_Score=390, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321112, Length=275, Percent_Identity=29.0909090909091, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 29470; Mature: 29470

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVALGIEYDGSQYFGWQRQAEVDTVQERLEKALSIVANEPIAVHCAGRTDAGVHATGQV
CEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCEE
VHFETNAIRKETAWTLGVNVNLPDNIAVRWVKVVDDEFHARFTATARRYRYMIYNHQLRP
EEEECCCCCCCCEEEEEEEECCCCCHHEEEEEEECCCHHHHHHHHHHHEEEEEEECCCCC
GILRSGVSHYPGDIDESKMHQAAQYLLGEQDFTSFRAVHCQSNTPFRCVHEVNVTRQGMY
HHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCCEE
ICVDIKANAFLHHMVRNIVGSLLEVGLGNQSVEWIEQLLALKDRNKAAATAKPNGLYLVD
EEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
VTYPESYQLPKLSLGPLFMLD
EECCCCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MRVALGIEYDGSQYFGWQRQAEVDTVQERLEKALSIVANEPIAVHCAGRTDAGVHATGQV
CEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCEE
VHFETNAIRKETAWTLGVNVNLPDNIAVRWVKVVDDEFHARFTATARRYRYMIYNHQLRP
EEEECCCCCCCCEEEEEEEECCCCCHHEEEEEEECCCHHHHHHHHHHHEEEEEEECCCCC
GILRSGVSHYPGDIDESKMHQAAQYLLGEQDFTSFRAVHCQSNTPFRCVHEVNVTRQGMY
HHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCCEE
ICVDIKANAFLHHMVRNIVGSLLEVGLGNQSVEWIEQLLALKDRNKAAATAKPNGLYLVD
EEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
VTYPESYQLPKLSLGPLFMLD
EECCCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA