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The map label for this gene is trpCF [H]

Identifier: 157961413

GI number: 157961413

Start: 1925488

End: 1926915

Strand: Reverse

Name: trpCF [H]

Synonym: Spea_1587

Alternate gene names: 157961413

Gene position: 1926915-1925488 (Counterclockwise)

Preceding gene: 157961414

Following gene: 157961412

Centisome position: 37.24

GC content: 46.71

Gene sequence:

>1428_bases
GTGAGCACGGATTTAAGAGAAGAACCAGCAATAAAAGAAAGCAATGTACTGACTAAAATTGTCGATACTAAGGCGGCACA
TATCGCCTCACTCAAGCAGCGATTTCCTGAAAAGGACTTATCGCCAAAAGTGTCTGACAGAAGTTTATTTGATGCACTTA
AAGCGCCAAATGCAGGTTTTATTCTAGAGTGTAAAAAAGCCAGCCCATCAAAAGGCTTAATTCGGGATGTGTTTGATGTT
GATGCTATAGCTGATGTTTATAACCGCTACGCCGCAGGTATTTCAGTATTAACCGACGAGCAGTTCTTCCAAGGCGACAT
GGACTATATTCCAAGGGTTCGTGCTCGCGTTAGCCAGCCGATTTTGTGTAAAGACTTTTTCGTCGATGAGTACCAAGTCA
AATTGGCCGCTCACCAAGGCGCTGACGCAATCTTGTTAATGCTATCGGTACTCGATGACGGCCGTTATCAAGCCCTAGCC
ACCGAAGCGGCGAAATATCAACTCGATATCTTGACCGAAGTGAGTAATGAAGCAGAACTCACGCGCGCCATTGCGCTTAA
TGCCAAGATTATCGGCATTAATAACCGTAATCTGCGCGACTTGTCTACCGACCTTGCAACCACAGAGGCGCTGGCGCCGC
ATATTCCATCCGACAGAGTGGTGATCAGCGAATCTGGTATTTATAACCAAGCTCAAGTTCGCCGTTTAAGCCCGCTAGTG
GATGGTTTCTTAGTCGGCAGCTCGTTAATGGCAGAAGATGATTTAGACCTTGCCTGCCGAACACTGACCTTTGGCCATAA
CAAAGTCTGCGGCCTAACCCGCATGGAAGACATGATAGCAGCCGCAAAGGCTGGTGCCATTTATGGCGGCCTTATCTTTG
CTCAAAAATCACCACGCGCGGTAACGCCTGAGCAGGCTAATAAGCTGGTTGCGCAGTTAAAGCAAAGCGGCACTAAACTC
AATTTAGTTGGCGTGTTTGTTAATGAAGCGGCCGATGTGATCGCCAAGCTCGCTACTGAACTAAACCTGTTTGCCGTGCA
GTTACACGGTAAAGAAACCGCACTCGAAATTGATGCATTAAAAGCAATATTGACCACAAACAATAGCCAGACTCAAATTT
GGAAAGCGGTTGCGGTAGATGTGGCAAGTGACAATGAATTAGTTATTCCTGCAGGCGTTGATCGCGTGGTGTTTGACAGT
AAATCAGATGGTCAATTTGGCGGTACGGGCCAAGCTTTTGATTGGCAAAAGATGCTACCAAACAAAGAGTCTGCATTACT
TGCCGGTGGTTTAAGCCCAGAGAATGCCATTGATGCAGCAAGCCAAGGTTTCTACGGCTTAGATTTTAACTCTGGCCTTG
AGCAAAGTCCTGGTATTAAAGACCCAAGCAAAGTAGCAGCGGCATTTAACCAGTTGCGCCAGTACTGA

Upstream 100 bases:

>100_bases
TCTGGCACAGCGTTAGCACTCGAGACCATTAACAGCGGCAAAGCCTACAAATTATTGACCCAGCTGGCTAACGCTAGCCA
AGCTGGCAAAGGAGCCTAAT

Downstream 100 bases:

>100_bases
TTTTATTAGCAGCTGTAAAAAGCTAGTTTCTAAACCCTATTATCCCCACATGCAAAATCGGCCTGTGGTAAAAAATTAGT
CATAAAGGACAAGAATATGA

Product: bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase

Products: NA

Alternate protein names: Indole-3-glycerol phosphate synthase; IGPS; N-(5'-phospho-ribosyl)anthranilate isomerase; PRAI [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSTDLREEPAIKESNVLTKIVDTKAAHIASLKQRFPEKDLSPKVSDRSLFDALKAPNAGFILECKKASPSKGLIRDVFDV
DAIADVYNRYAAGISVLTDEQFFQGDMDYIPRVRARVSQPILCKDFFVDEYQVKLAAHQGADAILLMLSVLDDGRYQALA
TEAAKYQLDILTEVSNEAELTRAIALNAKIIGINNRNLRDLSTDLATTEALAPHIPSDRVVISESGIYNQAQVRRLSPLV
DGFLVGSSLMAEDDLDLACRTLTFGHNKVCGLTRMEDMIAAAKAGAIYGGLIFAQKSPRAVTPEQANKLVAQLKQSGTKL
NLVGVFVNEAADVIAKLATELNLFAVQLHGKETALEIDALKAILTTNNSQTQIWKAVAVDVASDNELVIPAGVDRVVFDS
KSDGQFGGTGQAFDWQKMLPNKESALLAGGLSPENAIDAASQGFYGLDFNSGLEQSPGIKDPSKVAAAFNQLRQY

Sequences:

>Translated_475_residues
MSTDLREEPAIKESNVLTKIVDTKAAHIASLKQRFPEKDLSPKVSDRSLFDALKAPNAGFILECKKASPSKGLIRDVFDV
DAIADVYNRYAAGISVLTDEQFFQGDMDYIPRVRARVSQPILCKDFFVDEYQVKLAAHQGADAILLMLSVLDDGRYQALA
TEAAKYQLDILTEVSNEAELTRAIALNAKIIGINNRNLRDLSTDLATTEALAPHIPSDRVVISESGIYNQAQVRRLSPLV
DGFLVGSSLMAEDDLDLACRTLTFGHNKVCGLTRMEDMIAAAKAGAIYGGLIFAQKSPRAVTPEQANKLVAQLKQSGTKL
NLVGVFVNEAADVIAKLATELNLFAVQLHGKETALEIDALKAILTTNNSQTQIWKAVAVDVASDNELVIPAGVDRVVFDS
KSDGQFGGTGQAFDWQKMLPNKESALLAGGLSPENAIDAASQGFYGLDFNSGLEQSPGIKDPSKVAAAFNQLRQY
>Mature_474_residues
STDLREEPAIKESNVLTKIVDTKAAHIASLKQRFPEKDLSPKVSDRSLFDALKAPNAGFILECKKASPSKGLIRDVFDVD
AIADVYNRYAAGISVLTDEQFFQGDMDYIPRVRARVSQPILCKDFFVDEYQVKLAAHQGADAILLMLSVLDDGRYQALAT
EAAKYQLDILTEVSNEAELTRAIALNAKIIGINNRNLRDLSTDLATTEALAPHIPSDRVVISESGIYNQAQVRRLSPLVD
GFLVGSSLMAEDDLDLACRTLTFGHNKVCGLTRMEDMIAAAKAGAIYGGLIFAQKSPRAVTPEQANKLVAQLKQSGTKLN
LVGVFVNEAADVIAKLATELNLFAVQLHGKETALEIDALKAILTTNNSQTQIWKAVAVDVASDNELVIPAGVDRVVFDSK
SDGQFGGTGQAFDWQKMLPNKESALLAGGLSPENAIDAASQGFYGLDFNSGLEQSPGIKDPSKVAAAFNQLRQY

Specific function: Bifunctional enzyme that catalyzes two sequential steps of tryptophan biosynthetic pathway. The first reaction is catalyzed by the isomerase, coded by the trpF domain; the second reaction is catalyzed by the synthase, coded by the trpC domain [H]

COG id: COG0134

COG function: function code E; Indole-3-glycerol phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the trpF family [H]

Homologues:

Organism=Escherichia coli, GI87081863, Length=471, Percent_Identity=51.380042462845, Blast_Score=410, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6322638, Length=234, Percent_Identity=32.4786324786325, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320210, Length=218, Percent_Identity=30.2752293577982, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013798
- InterPro:   IPR001468
- InterPro:   IPR001240
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00218 IGPS; PF00697 PRAI [H]

EC number: =4.1.1.48; =5.3.1.24 [H]

Molecular weight: Translated: 51392; Mature: 51261

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00614 IGPS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDLREEPAIKESNVLTKIVDTKAAHIASLKQRFPEKDLSPKVSDRSLFDALKAPNAGF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCE
ILECKKASPSKGLIRDVFDVDAIADVYNRYAAGISVLTDEQFFQGDMDYIPRVRARVSQP
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHCCCHHHHHHHHHHCCCC
ILCKDFFVDEYQVKLAAHQGADAILLMLSVLDDGRYQALATEAAKYQLDILTEVSNEAEL
EEEHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHEEEHHHHCCCHHHH
TRAIALNAKIIGINNRNLRDLSTDLATTEALAPHIPSDRVVISESGIYNQAQVRRLSPLV
HHEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHH
DGFLVGSSLMAEDDLDLACRTLTFGHNKVCGLTRMEDMIAAAKAGAIYGGLIFAQKSPRA
HHHHHCCHHHCCCCCCCEEEEEECCCCCEECHHHHHHHHHHHHCCCEEEEEEEECCCCCC
VTPEQANKLVAQLKQSGTKLNLVGVFVNEAADVIAKLATELNLFAVQLHGKETALEIDAL
CCHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHCEEEEEEECCCCCEEHHHHH
KAILTTNNSQTQIWKAVAVDVASDNELVIPAGVDRVVFDSKSDGQFGGTGQAFDWQKMLP
HHHHCCCCCHHHEEEEEEEEECCCCCEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHCC
NKESALLAGGLSPENAIDAASQGFYGLDFNSGLEQSPGIKDPSKVAAAFNQLRQY
CCCCEEEECCCCCHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
STDLREEPAIKESNVLTKIVDTKAAHIASLKQRFPEKDLSPKVSDRSLFDALKAPNAGF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCE
ILECKKASPSKGLIRDVFDVDAIADVYNRYAAGISVLTDEQFFQGDMDYIPRVRARVSQP
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHCCCHHHHHHHHHHCCCC
ILCKDFFVDEYQVKLAAHQGADAILLMLSVLDDGRYQALATEAAKYQLDILTEVSNEAEL
EEEHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHEEEHHHHCCCHHHH
TRAIALNAKIIGINNRNLRDLSTDLATTEALAPHIPSDRVVISESGIYNQAQVRRLSPLV
HHEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHH
DGFLVGSSLMAEDDLDLACRTLTFGHNKVCGLTRMEDMIAAAKAGAIYGGLIFAQKSPRA
HHHHHCCHHHCCCCCCCEEEEEECCCCCEECHHHHHHHHHHHHCCCEEEEEEEECCCCCC
VTPEQANKLVAQLKQSGTKLNLVGVFVNEAADVIAKLATELNLFAVQLHGKETALEIDAL
CCHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHCEEEEEEECCCCCEEHHHHH
KAILTTNNSQTQIWKAVAVDVASDNELVIPAGVDRVVFDSKSDGQFGGTGQAFDWQKMLP
HHHHCCCCCHHHEEEEEEEEECCCCCEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHCC
NKESALLAGGLSPENAIDAASQGFYGLDFNSGLEQSPGIKDPSKVAAAFNQLRQY
CCCCEEEECCCCCHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]