Click here to switch to the map view.

The map label for this gene is gltT [H]

Identifier: 157961358

GI number: 157961358

Start: 1861254

End: 1862582

Strand: Reverse

Name: gltT [H]

Synonym: Spea_1532

Alternate gene names: 157961358

Gene position: 1862582-1861254 (Counterclockwise)

Preceding gene: 157961360

Following gene: 157961349

Centisome position: 35.99

GC content: 45.6

Gene sequence:

>1329_bases
ATGTCTGCTACGAAAACTAAAAAACTCGGCCTCACGAGTAAGATAATTATCGGTATGTTTACCGGTATTATTCTCGGACT
CTTACTACGCAATCTGTTTCCAGAGAGTGACTTTATCAAAGAGTACATCACTGAGGGTGTGCTAAATGTAATCGGTACTA
TCTTTATCTCAAGCTTGAAAATGCTTGTTGTTCCTCTTGTCTTTGTTTCATTAGTCTGTGGTACTTGTTCGCTAAGTGAA
CCCTCTAAAGTAGGTCGCTTAGGTGGCAAGACCATCGCGTTTTATCTATTCACCACAGCCATTGCCCTTTCAATGGCGAT
TCTAGTTGCCATTGCTATCCACCCTGGCAATGCCAGTATGGTGACTGAAGGAATGCAGTTTGACGTCAAGCAGGCCCCAA
GCCTTTCTGATGTACTTATTAATCTTGTGCCTACCAACCCACTGCATGCGATGAGTGAAGGTAATATGCTGCAGATCATC
ATCTTTGCGGTGATCTTTGGTTTTGCTATCTCGCATATTGGTGAACGTGGTGCGCGTGTCGCAGCACTGTTTAACGACCT
TAACGAAGTGATCATGCGTGTTGTAACACTTATCATGCAACTTGCTCCTTACGGCGTGTTTGCCCTGATGGCAAAATTGG
CGCTCACACTGGGGATGGAAACCTTTGGCAGTGTCGTTAAATACTTCTTCGTAGTACTAGGTGTGTTACTTGTTCATGCA
TTCGTGGTGTACCCGGTGCTGCTTAAAGTGTTCTCTGGGCTCAACCCATTCACCTTTATCCGTAAAATCCGTGACGTACA
GCTATTTGCATTTAGTACAGCGAGTTCAAACGCGACGCTGCCTGTGACTATTGAGACTGCAGAGCACCGTATGGGCGTGG
ATAATAAGATTGCCTCTTTCACCCTGCCACTTGGTGCGACTATTAACATGGATGGCACCGCGATCATGCAAGGCGTTGCG
ACCGTCTTTATTGCCCAAGTCTTTGGTATCGAGCTAACAATTACCGATTACGCTATGGTTGTTGTTACAGCAACGCTTGC
CTCTATCGGTACTGCCGGTGTACCTGGTGTGGGTCTAATCATGCTTGCCATGGTACTGAACCAAGTCGGTCTGCCTGTTG
AGGGTATCGCGCTGATTATTGGTGTAGATAGACTGCTGGATATGGTGCGTACAGCGGTTAACGTAACCGGTGATACAGTA
GCAACTGTGGTGATTGCTAAATCTGAAAATGAGTTTAACGAAGAGATCTTCAATGATACTCAAGCGGGTAAAGTCGCTCC
AGGTTTCAACGCTCAAGTTCATGCTGAAGAAATGAAGTTGAACAAGTAA

Upstream 100 bases:

>100_bases
ACCCGGTAAAACTTAACTTTTACCCCATTTCCTCGGTGTCACTTTCTTGCTAGGCTTTAGAACGCAAATTGAAAAGCATA
AAAATTATACAGGAAGCACT

Downstream 100 bases:

>100_bases
AAAGCTAGGTTCAATCTAAAAAGGCGTCCAATTGGACGCCTTTTTAATGACTTACTTTCGCTAGTTAAGGCAGCCATTTT
ACGCTGGCAAACTGCTAGCT

Product: sodium:dicarboxylate symporter

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSATKTKKLGLTSKIIIGMFTGIILGLLLRNLFPESDFIKEYITEGVLNVIGTIFISSLKMLVVPLVFVSLVCGTCSLSE
PSKVGRLGGKTIAFYLFTTAIALSMAILVAIAIHPGNASMVTEGMQFDVKQAPSLSDVLINLVPTNPLHAMSEGNMLQII
IFAVIFGFAISHIGERGARVAALFNDLNEVIMRVVTLIMQLAPYGVFALMAKLALTLGMETFGSVVKYFFVVLGVLLVHA
FVVYPVLLKVFSGLNPFTFIRKIRDVQLFAFSTASSNATLPVTIETAEHRMGVDNKIASFTLPLGATINMDGTAIMQGVA
TVFIAQVFGIELTITDYAMVVVTATLASIGTAGVPGVGLIMLAMVLNQVGLPVEGIALIIGVDRLLDMVRTAVNVTGDTV
ATVVIAKSENEFNEEIFNDTQAGKVAPGFNAQVHAEEMKLNK

Sequences:

>Translated_442_residues
MSATKTKKLGLTSKIIIGMFTGIILGLLLRNLFPESDFIKEYITEGVLNVIGTIFISSLKMLVVPLVFVSLVCGTCSLSE
PSKVGRLGGKTIAFYLFTTAIALSMAILVAIAIHPGNASMVTEGMQFDVKQAPSLSDVLINLVPTNPLHAMSEGNMLQII
IFAVIFGFAISHIGERGARVAALFNDLNEVIMRVVTLIMQLAPYGVFALMAKLALTLGMETFGSVVKYFFVVLGVLLVHA
FVVYPVLLKVFSGLNPFTFIRKIRDVQLFAFSTASSNATLPVTIETAEHRMGVDNKIASFTLPLGATINMDGTAIMQGVA
TVFIAQVFGIELTITDYAMVVVTATLASIGTAGVPGVGLIMLAMVLNQVGLPVEGIALIIGVDRLLDMVRTAVNVTGDTV
ATVVIAKSENEFNEEIFNDTQAGKVAPGFNAQVHAEEMKLNK
>Mature_441_residues
SATKTKKLGLTSKIIIGMFTGIILGLLLRNLFPESDFIKEYITEGVLNVIGTIFISSLKMLVVPLVFVSLVCGTCSLSEP
SKVGRLGGKTIAFYLFTTAIALSMAILVAIAIHPGNASMVTEGMQFDVKQAPSLSDVLINLVPTNPLHAMSEGNMLQIII
FAVIFGFAISHIGERGARVAALFNDLNEVIMRVVTLIMQLAPYGVFALMAKLALTLGMETFGSVVKYFFVVLGVLLVHAF
VVYPVLLKVFSGLNPFTFIRKIRDVQLFAFSTASSNATLPVTIETAEHRMGVDNKIASFTLPLGATINMDGTAIMQGVAT
VFIAQVFGIELTITDYAMVVVTATLASIGTAGVPGVGLIMLAMVLNQVGLPVEGIALIIGVDRLLDMVRTAVNVTGDTVA
TVVIAKSENEFNEEIFNDTQAGKVAPGFNAQVHAEEMKLNK

Specific function: This carrier protein is part of the Na(+)-dependent, binding-protein-independent glutamate-aspartate transport system [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=478, Percent_Identity=33.6820083682008, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI40254478, Length=508, Percent_Identity=30.511811023622, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI66773030, Length=400, Percent_Identity=34, Blast_Score=202, Evalue=7e-52,
Organism=Homo sapiens, GI21314632, Length=414, Percent_Identity=32.8502415458937, Blast_Score=198, Evalue=7e-51,
Organism=Homo sapiens, GI4827012, Length=334, Percent_Identity=37.7245508982036, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI5032093, Length=375, Percent_Identity=35.4666666666667, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI194239697, Length=292, Percent_Identity=37.6712328767123, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI223468564, Length=250, Percent_Identity=39.2, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI223468566, Length=259, Percent_Identity=38.996138996139, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI262359914, Length=371, Percent_Identity=32.0754716981132, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI301601644, Length=170, Percent_Identity=41.7647058823529, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1790514, Length=423, Percent_Identity=35.2245862884161, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1789947, Length=439, Percent_Identity=30.5239179954442, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1788024, Length=450, Percent_Identity=30.6666666666667, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789473, Length=385, Percent_Identity=29.0909090909091, Blast_Score=131, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI71983099, Length=458, Percent_Identity=32.3144104803493, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71983106, Length=458, Percent_Identity=32.3144104803493, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17537407, Length=419, Percent_Identity=35.799522673031, Blast_Score=224, Evalue=8e-59,
Organism=Caenorhabditis elegans, GI193206505, Length=481, Percent_Identity=32.4324324324324, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI71996953, Length=454, Percent_Identity=33.0396475770925, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI193206654, Length=447, Percent_Identity=29.7539149888143, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17541374, Length=392, Percent_Identity=30.8673469387755, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24583025, Length=445, Percent_Identity=31.9101123595506, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI17137668, Length=445, Percent_Identity=31.9101123595506, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24583023, Length=445, Percent_Identity=31.9101123595506, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI281360481, Length=448, Percent_Identity=29.9107142857143, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI281360483, Length=448, Percent_Identity=29.9107142857143, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17137666, Length=448, Percent_Identity=29.9107142857143, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 47200; Mature: 47069

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATKTKKLGLTSKIIIGMFTGIILGLLLRNLFPESDFIKEYITEGVLNVIGTIFISSLK
CCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MLVVPLVFVSLVCGTCSLSEPSKVGRLGGKTIAFYLFTTAIALSMAILVAIAIHPGNASM
HHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCHH
VTEGMQFDVKQAPSLSDVLINLVPTNPLHAMSEGNMLQIIIFAVIFGFAISHIGERGARV
HHCCCCCCHHCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHH
AALFNDLNEVIMRVVTLIMQLAPYGVFALMAKLALTLGMETFGSVVKYFFVVLGVLLVHA
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVVYPVLLKVFSGLNPFTFIRKIRDVQLFAFSTASSNATLPVTIETAEHRMGVDNKIASF
HHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEECCCCCEEEEEEECHHHHCCCCCCHHEE
TLPLGATINMDGTAIMQGVATVFIAQVFGIELTITDYAMVVVTATLASIGTAGVPGVGLI
EECCCCEECCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHHH
MLAMVLNQVGLPVEGIALIIGVDRLLDMVRTAVNVTGDTVATVVIAKSENEFNEEIFNDT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHCCC
QAGKVAPGFNAQVHAEEMKLNK
CCCCCCCCCCCEEEHHHHCCCC
>Mature Secondary Structure 
SATKTKKLGLTSKIIIGMFTGIILGLLLRNLFPESDFIKEYITEGVLNVIGTIFISSLK
CCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MLVVPLVFVSLVCGTCSLSEPSKVGRLGGKTIAFYLFTTAIALSMAILVAIAIHPGNASM
HHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCHH
VTEGMQFDVKQAPSLSDVLINLVPTNPLHAMSEGNMLQIIIFAVIFGFAISHIGERGARV
HHCCCCCCHHCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHH
AALFNDLNEVIMRVVTLIMQLAPYGVFALMAKLALTLGMETFGSVVKYFFVVLGVLLVHA
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVVYPVLLKVFSGLNPFTFIRKIRDVQLFAFSTASSNATLPVTIETAEHRMGVDNKIASF
HHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEECCCCCEEEEEEECHHHHCCCCCCHHEE
TLPLGATINMDGTAIMQGVATVFIAQVFGIELTITDYAMVVVTATLASIGTAGVPGVGLI
EECCCCEECCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHHH
MLAMVLNQVGLPVEGIALIIGVDRLLDMVRTAVNVTGDTVATVVIAKSENEFNEEIFNDT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHCCC
QAGKVAPGFNAQVHAEEMKLNK
CCCCCCCCCCCEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]