Click here to switch to the map view.

The map label for this gene is mak [H]

Identifier: 157961267

GI number: 157961267

Start: 1755727

End: 1756632

Strand: Direct

Name: mak [H]

Synonym: Spea_1441

Alternate gene names: 157961267

Gene position: 1755727-1756632 (Clockwise)

Preceding gene: 157961266

Following gene: 157961268

Centisome position: 33.93

GC content: 47.68

Gene sequence:

>906_bases
ATGTTGCGGATTGGTGTTGATCTTGGTGGTTCAAAAATTGAAGTTGTAGCACTTAACGAGCAGGGCACAGAGCTATTTCG
TAAACGTCTTCAGACACCAAGAGAATATAATGCAACGCTTGATGCGATAGAGGCTCTAGTGACTGAAGCCGAACTGCAAT
TAGGGCAAAAAGGCACTGTGGGCGTTGGGATCCCCGGCGTTGTGTCGCCATTTTCAGGTTTAGTTAAAAATGCCAATTCC
ACCTGGATTAACGGTCACCCACTCGATATCGATTTAGGTCGGCGTTTAAACAGAGAAGTCAGAGTGGCAAACGATGCCAA
TTGCTTTGCGGTCTCAGAGGCTATCGATGGTGCCGCGGCCGGAAAAACCGTGGTTTTTGGGGTGATTATAGGTACTGGAT
GCGGGGCTGGCATTGCCATTAACGGTAAAGTACATGGCGGTGGCAATGGTATTGGTGGTGAGTGGGGGCATAATCCTTTG
CCTTGGATGACCCCAGATGAGTTCAACTCAACCAGTTGTTTCTGCGGTAATAAAGATTGCATTGAGACTTTTATTTCTGG
TACTGGTTTTGTGAGGGACTATCAACTTGCAGGGGGGCTCGCGACAAATGGTGCTGAGATAGTTTCGTCTATGCAAGAAG
GTGATGTTATAGCTTGTGCTGCATTTGAGCGTTATGTCGATAGGTTGGCCCGTTCACTCGCTCATGTTATCAATGTGCTG
GATCCTGACATAATTGTGCTTGGCGGCGGCGTCTCGAATATTGAGGCTATTTACCCGTTATTGCCTGACTTCTTACCTAA
ATATGTATTGGGCGGAGAGTGTCGAACCCCTGTAGTGCAAAACATGTATGGTAGCGCTTCAGGCGTACGCGGAGCAGCTT
GGTTGTGGAGTAAAGATGAGACTTAG

Upstream 100 bases:

>100_bases
AGTTGACTTTAGCTGTGTTGGTGAGAGTAGGCGAGTCTGAGTAAAAGGCTACTCTCGGTTTAAATGTTAAAACGAATTAA
AAATACTTTTGGAGCGGGAA

Downstream 100 bases:

>100_bases
TAATCTTGACTGCTGCACTTATCCACTACAGTTTGATATTCTGGTTAGGCATGTAGCTTGCTCACTACTGAGGCTATCTA
TTTATGGTGTAGGCACGAAT

Product: fructokinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MLRIGVDLGGSKIEVVALNEQGTELFRKRLQTPREYNATLDAIEALVTEAELQLGQKGTVGVGIPGVVSPFSGLVKNANS
TWINGHPLDIDLGRRLNREVRVANDANCFAVSEAIDGAAAGKTVVFGVIIGTGCGAGIAINGKVHGGGNGIGGEWGHNPL
PWMTPDEFNSTSCFCGNKDCIETFISGTGFVRDYQLAGGLATNGAEIVSSMQEGDVIACAAFERYVDRLARSLAHVINVL
DPDIIVLGGGVSNIEAIYPLLPDFLPKYVLGGECRTPVVQNMYGSASGVRGAAWLWSKDET

Sequences:

>Translated_301_residues
MLRIGVDLGGSKIEVVALNEQGTELFRKRLQTPREYNATLDAIEALVTEAELQLGQKGTVGVGIPGVVSPFSGLVKNANS
TWINGHPLDIDLGRRLNREVRVANDANCFAVSEAIDGAAAGKTVVFGVIIGTGCGAGIAINGKVHGGGNGIGGEWGHNPL
PWMTPDEFNSTSCFCGNKDCIETFISGTGFVRDYQLAGGLATNGAEIVSSMQEGDVIACAAFERYVDRLARSLAHVINVL
DPDIIVLGGGVSNIEAIYPLLPDFLPKYVLGGECRTPVVQNMYGSASGVRGAAWLWSKDET
>Mature_301_residues
MLRIGVDLGGSKIEVVALNEQGTELFRKRLQTPREYNATLDAIEALVTEAELQLGQKGTVGVGIPGVVSPFSGLVKNANS
TWINGHPLDIDLGRRLNREVRVANDANCFAVSEAIDGAAAGKTVVFGVIIGTGCGAGIAINGKVHGGGNGIGGEWGHNPL
PWMTPDEFNSTSCFCGNKDCIETFISGTGFVRDYQLAGGLATNGAEIVSSMQEGDVIACAAFERYVDRLARSLAHVINVL
DPDIIVLGGGVSNIEAIYPLLPDFLPKYVLGGECRTPVVQNMYGSASGVRGAAWLWSKDET

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=302, Percent_Identity=58.9403973509934, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1787363, Length=304, Percent_Identity=41.4473684210526, Blast_Score=211, Evalue=3e-56,
Organism=Escherichia coli, GI1790522, Length=262, Percent_Identity=26.7175572519084, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786891, Length=247, Percent_Identity=27.1255060728745, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787878, Length=265, Percent_Identity=21.5094339622642, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 31707; Mature: 31707

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIGVDLGGSKIEVVALNEQGTELFRKRLQTPREYNATLDAIEALVTEAELQLGQKGTV
CEEEEEECCCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
GVGIPGVVSPFSGLVKNANSTWINGHPLDIDLGRRLNREVRVANDANCFAVSEAIDGAAA
CCCCCHHHHHHHHHHHCCCCCEECCCCCEEHHHHHCCCEEEECCCCCEEEEHHHHCCCCC
GKTVVFGVIIGTGCGAGIAINGKVHGGGNGIGGEWGHNPLPWMTPDEFNSTSCFCGNKDC
CCEEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHH
IETFISGTGFVRDYQLAGGLATNGAEIVSSMQEGDVIACAAFERYVDRLARSLAHVINVL
HHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHC
DPDIIVLGGGVSNIEAIYPLLPDFLPKYVLGGECRTPVVQNMYGSASGVRGAAWLWSKDE
CCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCEEEEECCCC
T
C
>Mature Secondary Structure
MLRIGVDLGGSKIEVVALNEQGTELFRKRLQTPREYNATLDAIEALVTEAELQLGQKGTV
CEEEEEECCCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
GVGIPGVVSPFSGLVKNANSTWINGHPLDIDLGRRLNREVRVANDANCFAVSEAIDGAAA
CCCCCHHHHHHHHHHHCCCCCEECCCCCEEHHHHHCCCEEEECCCCCEEEEHHHHCCCCC
GKTVVFGVIIGTGCGAGIAINGKVHGGGNGIGGEWGHNPLPWMTPDEFNSTSCFCGNKDC
CCEEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHH
IETFISGTGFVRDYQLAGGLATNGAEIVSSMQEGDVIACAAFERYVDRLARSLAHVINVL
HHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHC
DPDIIVLGGGVSNIEAIYPLLPDFLPKYVLGGECRTPVVQNMYGSASGVRGAAWLWSKDE
CCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCEEEEECCCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]