Click here to switch to the map view.

The map label for this gene is rfbB [H]

Identifier: 157961225

GI number: 157961225

Start: 1699269

End: 1700366

Strand: Direct

Name: rfbB [H]

Synonym: Spea_1398

Alternate gene names: 157961225

Gene position: 1699269-1700366 (Clockwise)

Preceding gene: 157961224

Following gene: 157961226

Centisome position: 32.84

GC content: 40.35

Gene sequence:

>1098_bases
ATGAAAATTTTAGTCACAGGTGGAGCTGGTTTCATCGGCTCTGCAGTCGTGCGTCATATCATCAATAATACACAAGATAG
TGTTATCAATGTCGATAAATTAACTTACGCTGGTAACCTCGAATCTTTATCAAGCATTGAGTCAAATGAACGTTATGTCT
TCGAACAAGTTGATATTTGTGACCGCGCCGAATTAGACCGAGTTTTCGCACAATGCCAACCCAATGCTGTTATGCATTTA
GCTGCAGAATCTCATGTTGATCGTTCTATCACCGGTCCCGCTGATTTCATCCAGACGAATATCGTCGGCACCTATACGCT
TCTTGAAGCAACAAGAGCGTATTGGAACACATTGTCAAAAGGTGCTAAGCAAGCATTTCGTTTCCATCATATATCAACCG
ATGAAGTCTATGGCGATTTACCGCACCCTGACGAAGTCGAAAGTGGAAAAGAGTTACCGTTATTTACAGAAACGACAGCA
TACGAACCTTCAAGCCCTTACTCTGCATCTAAGGCTAGTTCTGATCATCTTGTTCGTGCTTGGTTAAGAACTTATGGCTT
ACCAACGATAGTAACCAACTGTTCAAATAACTATGGTCCATACCACTTCCCTGAAAAGCTTATTCCATTAGTTATTTTAA
ATGCATTAGAAGGAAAACCACTACCTATCTACGGTAAAGGCGATCAAATTCGAGATTGGTTATATGTTGAAGACCACGCT
AGAGCACTTTATAAAGTCGTTACAGAGGGGCTTGTAGGCGAAACATACAATATCGGTGGTCATAACGAAAAACAGAACTT
AGAAGTCGTTCAAACCATTTGTTCAATCTTGGATTTTTTAGTTCCAAAGGAAACTAAGTATAGCCAACAAATTACTTATG
TAACTGATAGACCGGGACATGACAGACGGTATGCGATAGATTCAAGTAAAATGCAACGTGAACTCGGATGGACGCCAGTA
GAAACTTTTGAAACCGGCCTTAGAAAGACTATTGAATGGTATTTGACTAATAAAGAGTGGTGTCAACACGTGCAAGATGG
AAGTTACCAGCGTGAACGTCTAGGTGTAGAGTCAATGACTACAAAGGAAAAAGTGTAA

Upstream 100 bases:

>100_bases
CTTGGTTCTACAGGCTGAGATTGGAAAGTGTGATTATTCAATTCACAACGTCTAAATTGAACTTGTTTTTAGACAAGTAA
TACATCTTGGAAACATAAAA

Downstream 100 bases:

>100_bases
TGAAAGGTTTAGTATTAGCGGGAGGCAGTGGCACACGTTTATATCCCATCACAATGGGAATATCTAAACAATTGTTGCCA
GTATACGACAAACCGATGAT

Product: dTDP-glucose 4,6 dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKILVTGGAGFIGSAVVRHIINNTQDSVINVDKLTYAGNLESLSSIESNERYVFEQVDICDRAELDRVFAQCQPNAVMHL
AAESHVDRSITGPADFIQTNIVGTYTLLEATRAYWNTLSKGAKQAFRFHHISTDEVYGDLPHPDEVESGKELPLFTETTA
YEPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWLYVEDHA
RALYKVVTEGLVGETYNIGGHNEKQNLEVVQTICSILDFLVPKETKYSQQITYVTDRPGHDRRYAIDSSKMQRELGWTPV
ETFETGLRKTIEWYLTNKEWCQHVQDGSYQRERLGVESMTTKEKV

Sequences:

>Translated_365_residues
MKILVTGGAGFIGSAVVRHIINNTQDSVINVDKLTYAGNLESLSSIESNERYVFEQVDICDRAELDRVFAQCQPNAVMHL
AAESHVDRSITGPADFIQTNIVGTYTLLEATRAYWNTLSKGAKQAFRFHHISTDEVYGDLPHPDEVESGKELPLFTETTA
YEPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWLYVEDHA
RALYKVVTEGLVGETYNIGGHNEKQNLEVVQTICSILDFLVPKETKYSQQITYVTDRPGHDRRYAIDSSKMQRELGWTPV
ETFETGLRKTIEWYLTNKEWCQHVQDGSYQRERLGVESMTTKEKV
>Mature_365_residues
MKILVTGGAGFIGSAVVRHIINNTQDSVINVDKLTYAGNLESLSSIESNERYVFEQVDICDRAELDRVFAQCQPNAVMHL
AAESHVDRSITGPADFIQTNIVGTYTLLEATRAYWNTLSKGAKQAFRFHHISTDEVYGDLPHPDEVESGKELPLFTETTA
YEPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWLYVEDHA
RALYKVVTEGLVGETYNIGGHNEKQNLEVVQTICSILDFLVPKETKYSQQITYVTDRPGHDRRYAIDSSKMQRELGWTPV
ETFETGLRKTIEWYLTNKEWCQHVQDGSYQRERLGVESMTTKEKV

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=339, Percent_Identity=34.5132743362832, Blast_Score=204, Evalue=9e-53,
Organism=Homo sapiens, GI42516563, Length=348, Percent_Identity=25, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI56237023, Length=348, Percent_Identity=28.448275862069, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI56118217, Length=348, Percent_Identity=28.448275862069, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI189083684, Length=348, Percent_Identity=28.448275862069, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1788353, Length=350, Percent_Identity=79.1428571428572, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI48994969, Length=360, Percent_Identity=71.6666666666667, Blast_Score=543, Evalue=1e-156,
Organism=Escherichia coli, GI1786974, Length=359, Percent_Identity=26.7409470752089, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788366, Length=255, Percent_Identity=25.8823529411765, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=336, Percent_Identity=37.797619047619, Blast_Score=208, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI115532424, Length=331, Percent_Identity=32.6283987915408, Blast_Score=170, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI71982035, Length=366, Percent_Identity=26.775956284153, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=368, Percent_Identity=26.6304347826087, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=337, Percent_Identity=25.2225519287834, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=371, Percent_Identity=26.1455525606469, Blast_Score=90, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19923002, Length=367, Percent_Identity=26.158038147139, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356223, Length=354, Percent_Identity=24.8587570621469, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 41289; Mature: 41289

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSAVVRHIINNTQDSVINVDKLTYAGNLESLSSIESNERYVFEQVDIC
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEHHHEEECCCHHHHHHCCCCCCEEEEHHCCC
DRAELDRVFAQCQPNAVMHLAAESHVDRSITGPADFIQTNIVGTYTLLEATRAYWNTLSK
CHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
GAKQAFRFHHISTDEVYGDLPHPDEVESGKELPLFTETTAYEPSSPYSASKASSDHLVRA
HHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHH
WLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWLYVEDHA
HHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHEEEEHHHH
RALYKVVTEGLVGETYNIGGHNEKQNLEVVQTICSILDFLVPKETKYSQQITYVTDRPGH
HHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCC
DRRYAIDSSKMQRELGWTPVETFETGLRKTIEWYLTNKEWCQHVQDGSYQRERLGVESMT
CCEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHCCHHHHH
TKEKV
HCCCC
>Mature Secondary Structure
MKILVTGGAGFIGSAVVRHIINNTQDSVINVDKLTYAGNLESLSSIESNERYVFEQVDIC
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEHHHEEECCCHHHHHHCCCCCCEEEEHHCCC
DRAELDRVFAQCQPNAVMHLAAESHVDRSITGPADFIQTNIVGTYTLLEATRAYWNTLSK
CHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
GAKQAFRFHHISTDEVYGDLPHPDEVESGKELPLFTETTAYEPSSPYSASKASSDHLVRA
HHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHH
WLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWLYVEDHA
HHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHEEEEHHHH
RALYKVVTEGLVGETYNIGGHNEKQNLEVVQTICSILDFLVPKETKYSQQITYVTDRPGH
HHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCC
DRRYAIDSSKMQRELGWTPVETFETGLRKTIEWYLTNKEWCQHVQDGSYQRERLGVESMT
CCEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHCCHHHHH
TKEKV
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503 [H]