Click here to switch to the map view.

The map label for this gene is rmlA2 [H]

Identifier: 157961226

GI number: 157961226

Start: 1700366

End: 1701238

Strand: Direct

Name: rmlA2 [H]

Synonym: Spea_1399

Alternate gene names: 157961226

Gene position: 1700366-1701238 (Clockwise)

Preceding gene: 157961225

Following gene: 157961227

Centisome position: 32.86

GC content: 36.66

Gene sequence:

>873_bases
ATGAAAGGTTTAGTATTAGCGGGAGGCAGTGGCACACGTTTATATCCCATCACAATGGGAATATCTAAACAATTGTTGCC
AGTATACGACAAACCGATGATTTACTATCCGATCTCAGTATTAATGTTGGCTGGTATTCGCGAGATCTTAATCATTACTA
CGCCAGAGGATAATGCAAGTTTTAAAAGATTATTAGGAGATGGTAGTCAGTTTGGTATTGAAATTGAGTATGCAATTCAA
CATAGTCCTGATGGTTTAGCACAAGCCTTTATTATTGGTGAAGAGTTTATCGGAGAAGACTCTGTTTGTTTAGTCCTTGG
TGATAATATTTTTTATGGTCAAGGCTTTTCGCCAATGCTTATTGACGCTTCAAAAAAAGAAGTCGGGGCTACAGTATTTG
GCTATAAAGTAAAAGATCCAGAACGTTTTGGTGTTGTTGATTTCAACACAGATATGAAAGCAGTATCGATTGAAGAAAAA
CCCGCAAAGCCAAAATCAAATTACGCTGTAACAGGTTTATACTTCTATGATAATCAAGTTGTAAATATTGCCAAAACAAT
TAAACCTTCTGAGCGCGGAGAGTTGGAAATAACTTCTATTAACCAAGTTTACTTAGAAAAAGGTGAATTGAATGTTGAGT
TATTAGGAAGGGGCTTCGCTTGGTTGGATACTGGAACTTATGAAAGTTTATTAGAGGCGGGACAGTTTGTAGCGACTGTT
GAGCACCGTCAAGGGAATAAAATAGCTTGTCTTGAAGAGATTGCATTAAATAATAATTGGTTGAACACTAAACAGGTTAT
AGCCCTTGCTGACCCTTTAAGAAAAAATAGCTATGGTCAATATCTACTGGACTTGTGCAGGAATGAATTATGA

Upstream 100 bases:

>100_bases
GGTATTTGACTAATAAAGAGTGGTGTCAACACGTGCAAGATGGAAGTTACCAGCGTGAACGTCTAGGTGTAGAGTCAATG
ACTACAAAGGAAAAAGTGTA

Downstream 100 bases:

>100_bases
GTTTAATAAAAACAATACAATTTAATAAGTTCGGTGATGAACGAGGGAGCTTGGTCTCACTAGAAAGTTTTGAGAATATT
CCTTTCGATATCAAACGAGT

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 2; dTDP-glucose pyrophosphorylase 2; dTDP-glucose synthase 2 [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MKGLVLAGGSGTRLYPITMGISKQLLPVYDKPMIYYPISVLMLAGIREILIITTPEDNASFKRLLGDGSQFGIEIEYAIQ
HSPDGLAQAFIIGEEFIGEDSVCLVLGDNIFYGQGFSPMLIDASKKEVGATVFGYKVKDPERFGVVDFNTDMKAVSIEEK
PAKPKSNYAVTGLYFYDNQVVNIAKTIKPSERGELEITSINQVYLEKGELNVELLGRGFAWLDTGTYESLLEAGQFVATV
EHRQGNKIACLEEIALNNNWLNTKQVIALADPLRKNSYGQYLLDLCRNEL

Sequences:

>Translated_290_residues
MKGLVLAGGSGTRLYPITMGISKQLLPVYDKPMIYYPISVLMLAGIREILIITTPEDNASFKRLLGDGSQFGIEIEYAIQ
HSPDGLAQAFIIGEEFIGEDSVCLVLGDNIFYGQGFSPMLIDASKKEVGATVFGYKVKDPERFGVVDFNTDMKAVSIEEK
PAKPKSNYAVTGLYFYDNQVVNIAKTIKPSERGELEITSINQVYLEKGELNVELLGRGFAWLDTGTYESLLEAGQFVATV
EHRQGNKIACLEEIALNNNWLNTKQVIALADPLRKNSYGQYLLDLCRNEL
>Mature_290_residues
MKGLVLAGGSGTRLYPITMGISKQLLPVYDKPMIYYPISVLMLAGIREILIITTPEDNASFKRLLGDGSQFGIEIEYAIQ
HSPDGLAQAFIIGEEFIGEDSVCLVLGDNIFYGQGFSPMLIDASKKEVGATVFGYKVKDPERFGVVDFNTDMKAVSIEEK
PAKPKSNYAVTGLYFYDNQVVNIAKTIKPSERGELEITSINQVYLEKGELNVELLGRGFAWLDTGTYESLLEAGQFVATV
EHRQGNKIACLEEIALNNNWLNTKQVIALADPLRKNSYGQYLLDLCRNEL

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=256, Percent_Identity=24.21875, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI11761619, Length=256, Percent_Identity=24.21875, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1790224, Length=287, Percent_Identity=71.7770034843206, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1788351, Length=287, Percent_Identity=64.4599303135889, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI1787488, Length=242, Percent_Identity=25.6198347107438, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI133931050, Length=238, Percent_Identity=25.2100840336134, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320148, Length=282, Percent_Identity=28.0141843971631, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355443, Length=252, Percent_Identity=23.4126984126984, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24644084, Length=252, Percent_Identity=23.4126984126984, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32124; Mature: 32124

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00014 ER_TARGET

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLVLAGGSGTRLYPITMGISKQLLPVYDKPMIYYPISVLMLAGIREILIITTPEDNAS
CCCEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEEEECCCCCHH
FKRLLGDGSQFGIEIEYAIQHSPDGLAQAFIIGEEFIGEDSVCLVLGDNIFYGQGFSPML
HHHHHCCCCCEEEEEEEEEECCCCCCEEEEEECHHHCCCCCEEEEEECCEEECCCCCCEE
IDASKKEVGATVFGYKVKDPERFGVVDFNTDMKAVSIEEKPAKPKSNYAVTGLYFYDNQV
EECCCHHCCEEEEEEEECCCCCEEEEEECCCCEEEEECCCCCCCCCCEEEEEEEEECCEE
VNIAKTIKPSERGELEITSINQVYLEKGELNVELLGRGFAWLDTGTYESLLEAGQFVATV
EEEEECCCCCCCCCEEEEECCEEEEECCCEEEEEEECCEEEEECCCHHHHHHCCCEEEEE
EHRQGNKIACLEEIALNNNWLNTKQVIALADPLRKNSYGQYLLDLCRNEL
ECCCCCEEEEEEHHHCCCCCCCHHEEEEEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKGLVLAGGSGTRLYPITMGISKQLLPVYDKPMIYYPISVLMLAGIREILIITTPEDNAS
CCCEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEEEECCCCCHH
FKRLLGDGSQFGIEIEYAIQHSPDGLAQAFIIGEEFIGEDSVCLVLGDNIFYGQGFSPML
HHHHHCCCCCEEEEEEEEEECCCCCCEEEEEECHHHCCCCCEEEEEECCEEECCCCCCEE
IDASKKEVGATVFGYKVKDPERFGVVDFNTDMKAVSIEEKPAKPKSNYAVTGLYFYDNQV
EECCCHHCCEEEEEEEECCCCCEEEEEECCCCEEEEECCCCCCCCCCEEEEEEEEECCEE
VNIAKTIKPSERGELEITSINQVYLEKGELNVELLGRGFAWLDTGTYESLLEAGQFVATV
EEEEECCCCCCCCCEEEEECCEEEEECCCEEEEEEECCEEEEECCCHHHHHHCCCEEEEE
EHRQGNKIACLEEIALNNNWLNTKQVIALADPLRKNSYGQYLLDLCRNEL
ECCCCCEEEEEEHHHCCCCCCCHHEEEEEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]