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The map label for this gene is rlmN [H]

Identifier: 157961130

GI number: 157961130

Start: 1586212

End: 1587333

Strand: Direct

Name: rlmN [H]

Synonym: Spea_1302

Alternate gene names: 157961130

Gene position: 1586212-1587333 (Clockwise)

Preceding gene: 157961127

Following gene: 157961131

Centisome position: 30.65

GC content: 43.23

Gene sequence:

>1122_bases
ATGAGTGAAAAGAAGATCAATTTATTGGATCTTGATCGTAAAGCATTAAGAGCGCTATTTACCGAAATGGGTGAAAAGCC
ATTCCGTGCTGATCAATTGATGAAATGGATTTATCATTTTGGCGTTAGTGACTTCGAAGAAATGACGAACATTAACAAAG
TATTGCGCGCTAAACTTGCCGCGAAATGCGAAATCGTCGCCCCTGAAATTTCAAGTTATCAAAAGTCTGTTGATGGCACA
ATTAAGTTCGCTATCAACGTTGGTGATGGTCAAGAAGTTGAAACGGTTTATATCCCTGAAGATGACCGTGCCACGCTATG
TGTATCGTCGCAAGTGGGTTGTGCCCTAGAGTGTACTTTCTGCTCAACGGCGCAGCAAGGCTTTAACCGTAACCTAACGG
TTGCAGAGATCGTAGGCCAAATTTGGCGCGTTGCCGACTTTATCGGTTTCGTGAAAGACACAGGTGAACGTCCAATCACA
AACGTTGTGATGATGGGCATGGGTGAACCATTACTCAACTTAAAGAATGTGATCCCAGCGATGGATATCATGTTGGATGA
TTTTGGTTTCAGCCTATCTAAGCGCCGTGTAACCTTATCGACATCAGGTGTTGTACCAGCTCTTGATAAATTGGGTGATG
TACTAGACGTCGCATTAGCCGTGAGTATTCACGCACCTAATGATGAGCTGCGTGACGTATTGGTTCCAGTTAACAAAAAA
TATCCACTAGAAGAGTTCTTAGGGGGTATTCGTCGCTATATCGCTAAATCTAATGCTAACCGCGGTCGCGTCACCGTTGA
GTACGTCATGCTAGATCATATTAATGACAGTACAGATCAGGCTCATGAGTTAGCTAAGCTGATGAAAGATACGCCGTGTA
AGGTTAACCTTATTCCGTTTAACCCATACCCGGGTTCTCCATATGGGCGTTCATCGAACTCTCGTATCGATAGATTCTCG
AAAGTCTTGATGGAATATGGCCTAACCGTTATCGTTCGTAAGACTCGTGGTGACGATATCGATGCTGCATGTGGTCAGTT
AGCGGGTGACATTCGCGATAGAACCAAACGTTTAGCAAAAAAACAAATGCAACAAAATCAAATTTCAGTCACAATAAACT
AA

Upstream 100 bases:

>100_bases
GCTTTTTAACTTTGGTGATAGCCCACTGGCTAATATTTGTTAAGATCTGTATAATTCGCCGCCCGCAAGCAGGTTTTAGG
TTTTTTTGAGGTTAGACTAA

Downstream 100 bases:

>100_bases
CTCTTTGTATCTACTGATAGATGACTGTTAGATGGGCTTAAAAATGAATCAGAGTAAGCTGGTATTGCCGCTGGTATTAA
TCCTGTCGTCAGTGATGACA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSEKKINLLDLDRKALRALFTEMGEKPFRADQLMKWIYHFGVSDFEEMTNINKVLRAKLAAKCEIVAPEISSYQKSVDGT
IKFAINVGDGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTAQQGFNRNLTVAEIVGQIWRVADFIGFVKDTGERPIT
NVVMMGMGEPLLNLKNVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDVLDVALAVSIHAPNDELRDVLVPVNKK
YPLEEFLGGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKVNLIPFNPYPGSPYGRSSNSRIDRFS
KVLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKQMQQNQISVTIN

Sequences:

>Translated_373_residues
MSEKKINLLDLDRKALRALFTEMGEKPFRADQLMKWIYHFGVSDFEEMTNINKVLRAKLAAKCEIVAPEISSYQKSVDGT
IKFAINVGDGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTAQQGFNRNLTVAEIVGQIWRVADFIGFVKDTGERPIT
NVVMMGMGEPLLNLKNVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDVLDVALAVSIHAPNDELRDVLVPVNKK
YPLEEFLGGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKVNLIPFNPYPGSPYGRSSNSRIDRFS
KVLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKQMQQNQISVTIN
>Mature_372_residues
SEKKINLLDLDRKALRALFTEMGEKPFRADQLMKWIYHFGVSDFEEMTNINKVLRAKLAAKCEIVAPEISSYQKSVDGTI
KFAINVGDGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTAQQGFNRNLTVAEIVGQIWRVADFIGFVKDTGERPITN
VVMMGMGEPLLNLKNVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDVLDVALAVSIHAPNDELRDVLVPVNKKY
PLEEFLGGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKVNLIPFNPYPGSPYGRSSNSRIDRFSK
VLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKQMQQNQISVTIN

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=366, Percent_Identity=74.3169398907104, Blast_Score=591, Evalue=1e-170,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41590; Mature: 41458

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKKINLLDLDRKALRALFTEMGEKPFRADQLMKWIYHFGVSDFEEMTNINKVLRAKLA
CCCCCCCHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AKCEIVAPEISSYQKSVDGTIKFAINVGDGQEVETVYIPEDDRATLCVSSQVGCALECTF
HCEEEECCCHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEE
CSTAQQGFNRNLTVAEIVGQIWRVADFIGFVKDTGERPITNVVMMGMGEPLLNLKNVIPA
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHH
MDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDVLDVALAVSIHAPNDELRDVLVPVNKK
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHEEEEECCCHHHHHHHCCCCCC
YPLEEFLGGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKVNLIPF
CCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEC
NPYPGSPYGRSSNSRIDRFSKVLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAK
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KQMQQNQISVTIN
HHHHCCCEEEEEC
>Mature Secondary Structure 
SEKKINLLDLDRKALRALFTEMGEKPFRADQLMKWIYHFGVSDFEEMTNINKVLRAKLA
CCCCCCHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AKCEIVAPEISSYQKSVDGTIKFAINVGDGQEVETVYIPEDDRATLCVSSQVGCALECTF
HCEEEECCCHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEE
CSTAQQGFNRNLTVAEIVGQIWRVADFIGFVKDTGERPITNVVMMGMGEPLLNLKNVIPA
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHH
MDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDVLDVALAVSIHAPNDELRDVLVPVNKK
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHEEEEECCCHHHHHHHCCCCCC
YPLEEFLGGIRRYIAKSNANRGRVTVEYVMLDHINDSTDQAHELAKLMKDTPCKVNLIPF
CCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEC
NPYPGSPYGRSSNSRIDRFSKVLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAK
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KQMQQNQISVTIN
HHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA