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The map label for this gene is pheA [H]

Identifier: 157960887

GI number: 157960887

Start: 1282497

End: 1284470

Strand: Reverse

Name: pheA [H]

Synonym: Spea_1059

Alternate gene names: 157960887

Gene position: 1284470-1282497 (Counterclockwise)

Preceding gene: 157960888

Following gene: 157960875

Centisome position: 24.82

GC content: 45.04

Gene sequence:

>1974_bases
ATGAGTAAACCACAACCCTTAAACCACACTCGAGAGCAGATCACTAGCCTCGATAATGAACTACTGGCCCTGCTTGCTAA
GCGCCGTGAATTAAGTTTAGATGTCGCCCGCAGTAAAGAGGTTGATGTTAGGCCTATACGCGATACCATTCGAGAAAAAG
AGCTGTTATCTCGCTTGGTGAAACAAGGCCGAGAACAAGGTTTAGATGCCCACTACGTTATATCTCTTTATCAAAGCATT
ATCGAAGACTCTGTACTTAACCAACAAGCCTATTTGCATGGCCGTGCAAACCCAGAAACTCAACAGCAACAATATTGTAT
CGCCTACTTAGGGGCGCGCGGCTCTTATTCATATCTTGCAGCGAGTCGTTACTGCGATAGACGCCAAGTAGAGATGCAAG
ATTTAGGCTGCCAAAGTTTTGATGAAATCGTTCAGGCCGTCGAATCTGGTCACGCGGATTATGGTTTCCTACCCATTGAG
AACACTTCATCAGGCTCAATCAACGAGGTTTATGATGTGTTACAGCACACCAGCCTTGCCATTGTCGGTGAAACCACTAT
TGAAGTTGGCCATTGTTTATTAGCAAAGAGCGGCAGCAGCATTAACGATATCAAAACCGTTTACGCCCACCCACAGCCAA
TAAGTCAGTGCAGTCGATACCTGAGCCAACATGGTGAATTCAAACTCGAGTATTGCTCAAGCAGCGCAGAAGCGATGGAG
ATGGTATGCAATGCTAATGATAACAGCGTGGCTGCTATCGGCAGCGCTGAAGGTGGCGCTCTATATCAATTAGAAGCCGT
TGAAAGTGGACTTGCCAATCAAAAAATCAATCAAAGCCGCTTTATTGTTGTTGCAAGAAAAGCTGTTGAAGTGCCTTCTC
AGCTTCCAGCTAAGTGCACACTGATTATGGCGACAGGGCAAAAGCCTGGCGCATTGGTTGAAGCCTTACTCGTACTTAAA
GCCCGAAACCTTAATATGAGCAAACTCGAGTCGCGTCCAATTCCTGGTACCCCATGGGAAGAGATGTTCTATCTCGATAT
TGATGCCAACTTGTCGAGTGAGCCAATGCAGGCAGCGCTGAAAGAGTTAGAAAGAACCACTCGCTTTATCAAGGTGCTTG
GCTGCTACCCATGTGAAACGGTTAAACCAACTCAACTAAGTAATAGTCAATTAATGATAGAGCCTGACACTTCTAAGTCG
ACCACGGTTACCGCTACGCCGCCAAACAAACAGATGCGCGTGAGTAAGCAATACAAGAGTGAGTCAACTCAAGTGATTTG
CGGCCAACTGAGCCTTGGTAATGGTAACTTAGGAGCTATAGCTCAAGTACAACTGCCACTCGATTTACAGCAATTTGAAC
AAATGGCAAAAGAGCTTAAAGAGTCTGGATTCCAGGCGGTTGTTATTCAGGGCTTGAGTCAGCAGAGTGATCTTATTAGC
TCGATAGAAAAGTTTAAGCATGCCCTAGGCCAATTCAATCTAGTTTGTGTGTTAGCTATTGAGCACGAAACAGATTTAAG
CATGGCAATTCAATTTGCCGATGTGGTGATGCTTGCAGGTAACTTGATGTATAACCAAGCCATACTAAGCCAACTCGGTA
GCTTACTGGTCCCGGTCATTTTAGAGCGTAATGCAATGGCAAGTGTAGATGACTTGCTTAATGCCGCTGAAGAAGTTTTA
AGTCAGGGCAATCAGCAGCTTATACTTTGCGAGTCAGGCGTAACCACACTTAACAATTCAGGCAAACCATCGCTAGATTT
GGCCGCATTAGTTGAGTTAAAAGCCCTAAGTCACTTACCGGTTGTTGTTAACCCAAGTTATGCCATTGATACCGATCAGC
TAGCAACATTTGCTAAAGCAATTAAGCAGCTTAAAGGTGATGGTGTCATGATGAACCTAAGCGCGATAGATACAGCGGCT
CAGGCTCATCCAGAGGAACTCGTTAAAGCAGTATTAGGTAATTTGTACCACTAG

Upstream 100 bases:

>100_bases
TCCAAAGCCTCCCACATGGGAGGCTTTTTTATATCAGCAAAGATAAGCTGTATCTTCATGCTTATTGCTACGGATATTAG
AAATAGATTCAGAGGCTAAG

Downstream 100 bases:

>100_bases
CCAATACTCGCGATGACCTGCAACAGCTATGAGTCAGAGCAGAGCGAATCTTGAGTAAGTGTTCAAATTAGTACAGATTA
AGGTTTACGCTGAGCTGCAT

Product: chorismate mutase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 657; Mature: 656

Protein sequence:

>657_residues
MSKPQPLNHTREQITSLDNELLALLAKRRELSLDVARSKEVDVRPIRDTIREKELLSRLVKQGREQGLDAHYVISLYQSI
IEDSVLNQQAYLHGRANPETQQQQYCIAYLGARGSYSYLAASRYCDRRQVEMQDLGCQSFDEIVQAVESGHADYGFLPIE
NTSSGSINEVYDVLQHTSLAIVGETTIEVGHCLLAKSGSSINDIKTVYAHPQPISQCSRYLSQHGEFKLEYCSSSAEAME
MVCNANDNSVAAIGSAEGGALYQLEAVESGLANQKINQSRFIVVARKAVEVPSQLPAKCTLIMATGQKPGALVEALLVLK
ARNLNMSKLESRPIPGTPWEEMFYLDIDANLSSEPMQAALKELERTTRFIKVLGCYPCETVKPTQLSNSQLMIEPDTSKS
TTVTATPPNKQMRVSKQYKSESTQVICGQLSLGNGNLGAIAQVQLPLDLQQFEQMAKELKESGFQAVVIQGLSQQSDLIS
SIEKFKHALGQFNLVCVLAIEHETDLSMAIQFADVVMLAGNLMYNQAILSQLGSLLVPVILERNAMASVDDLLNAAEEVL
SQGNQQLILCESGVTTLNNSGKPSLDLAALVELKALSHLPVVVNPSYAIDTDQLATFAKAIKQLKGDGVMMNLSAIDTAA
QAHPEELVKAVLGNLYH

Sequences:

>Translated_657_residues
MSKPQPLNHTREQITSLDNELLALLAKRRELSLDVARSKEVDVRPIRDTIREKELLSRLVKQGREQGLDAHYVISLYQSI
IEDSVLNQQAYLHGRANPETQQQQYCIAYLGARGSYSYLAASRYCDRRQVEMQDLGCQSFDEIVQAVESGHADYGFLPIE
NTSSGSINEVYDVLQHTSLAIVGETTIEVGHCLLAKSGSSINDIKTVYAHPQPISQCSRYLSQHGEFKLEYCSSSAEAME
MVCNANDNSVAAIGSAEGGALYQLEAVESGLANQKINQSRFIVVARKAVEVPSQLPAKCTLIMATGQKPGALVEALLVLK
ARNLNMSKLESRPIPGTPWEEMFYLDIDANLSSEPMQAALKELERTTRFIKVLGCYPCETVKPTQLSNSQLMIEPDTSKS
TTVTATPPNKQMRVSKQYKSESTQVICGQLSLGNGNLGAIAQVQLPLDLQQFEQMAKELKESGFQAVVIQGLSQQSDLIS
SIEKFKHALGQFNLVCVLAIEHETDLSMAIQFADVVMLAGNLMYNQAILSQLGSLLVPVILERNAMASVDDLLNAAEEVL
SQGNQQLILCESGVTTLNNSGKPSLDLAALVELKALSHLPVVVNPSYAIDTDQLATFAKAIKQLKGDGVMMNLSAIDTAA
QAHPEELVKAVLGNLYH
>Mature_656_residues
SKPQPLNHTREQITSLDNELLALLAKRRELSLDVARSKEVDVRPIRDTIREKELLSRLVKQGREQGLDAHYVISLYQSII
EDSVLNQQAYLHGRANPETQQQQYCIAYLGARGSYSYLAASRYCDRRQVEMQDLGCQSFDEIVQAVESGHADYGFLPIEN
TSSGSINEVYDVLQHTSLAIVGETTIEVGHCLLAKSGSSINDIKTVYAHPQPISQCSRYLSQHGEFKLEYCSSSAEAMEM
VCNANDNSVAAIGSAEGGALYQLEAVESGLANQKINQSRFIVVARKAVEVPSQLPAKCTLIMATGQKPGALVEALLVLKA
RNLNMSKLESRPIPGTPWEEMFYLDIDANLSSEPMQAALKELERTTRFIKVLGCYPCETVKPTQLSNSQLMIEPDTSKST
TVTATPPNKQMRVSKQYKSESTQVICGQLSLGNGNLGAIAQVQLPLDLQQFEQMAKELKESGFQAVVIQGLSQQSDLISS
IEKFKHALGQFNLVCVLAIEHETDLSMAIQFADVVMLAGNLMYNQAILSQLGSLLVPVILERNAMASVDDLLNAAEEVLS
QGNQQLILCESGVTTLNNSGKPSLDLAALVELKALSHLPVVVNPSYAIDTDQLATFAKAIKQLKGDGVMMNLSAIDTAAQ
AHPEELVKAVLGNLYH

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=386, Percent_Identity=54.4041450777202, Blast_Score=427, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6324013, Length=290, Percent_Identity=24.1379310344828, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010952
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 71845; Mature: 71714

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00858 PREPHENATE_DEHYDR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPQPLNHTREQITSLDNELLALLAKRRELSLDVARSKEVDVRPIRDTIREKELLSRLV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHH
KQGREQGLDAHYVISLYQSIIEDSVLNQQAYLHGRANPETQQQQYCIAYLGARGSYSYLA
HHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHEECCCCCCHHHHHHHHHEECCCCCHHHHH
ASRYCDRRQVEMQDLGCQSFDEIVQAVESGHADYGFLPIENTSSGSINEVYDVLQHTSLA
HHHCCCHHCCHHHHCCCCHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHCCEE
IVGETTIEVGHCLLAKSGSSINDIKTVYAHPQPISQCSRYLSQHGEFKLEYCSSSAEAME
EEECCHHHHHHEEEECCCCCCCHHHHEEECCCCHHHHHHHHHHCCCEEEEECCCHHHHHH
MVCNANDNSVAAIGSAEGGALYQLEAVESGLANQKINQSRFIVVARKAVEVPSQLPAKCT
HHHCCCCCCEEEEECCCCCCEEEEHHHHCCHHCCCCCCCEEEEEEHHHHCCCCCCCCEEE
LIMATGQKPGALVEALLVLKARNLNMSKLESRPIPGTPWEEMFYLDIDANLSSEPMQAAL
EEEECCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHEEEEEECCCCCCCHHHHHH
KELERTTRFIKVLGCYPCETVKPTQLSNSQLMIEPDTSKSTTVTATPPNKQMRVSKQYKS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCHHHHHHHHCC
ESTQVICGQLSLGNGNLGAIAQVQLPLDLQQFEQMAKELKESGFQAVVIQGLSQQSDLIS
CCCEEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEHHCCCHHHHHHH
SIEKFKHALGQFNLVCVLAIEHETDLSMAIQFADVVMLAGNLMYNQAILSQLGSLLVPVI
HHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERNAMASVDDLLNAAEEVLSQGNQQLILCESGVTTLNNSGKPSLDLAALVELKALSHLP
HCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCC
VVVNPSYAIDTDQLATFAKAIKQLKGDGVMMNLSAIDTAAQAHPEELVKAVLGNLYH
EEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKPQPLNHTREQITSLDNELLALLAKRRELSLDVARSKEVDVRPIRDTIREKELLSRLV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHH
KQGREQGLDAHYVISLYQSIIEDSVLNQQAYLHGRANPETQQQQYCIAYLGARGSYSYLA
HHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHEECCCCCCHHHHHHHHHEECCCCCHHHHH
ASRYCDRRQVEMQDLGCQSFDEIVQAVESGHADYGFLPIENTSSGSINEVYDVLQHTSLA
HHHCCCHHCCHHHHCCCCHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHCCEE
IVGETTIEVGHCLLAKSGSSINDIKTVYAHPQPISQCSRYLSQHGEFKLEYCSSSAEAME
EEECCHHHHHHEEEECCCCCCCHHHHEEECCCCHHHHHHHHHHCCCEEEEECCCHHHHHH
MVCNANDNSVAAIGSAEGGALYQLEAVESGLANQKINQSRFIVVARKAVEVPSQLPAKCT
HHHCCCCCCEEEEECCCCCCEEEEHHHHCCHHCCCCCCCEEEEEEHHHHCCCCCCCCEEE
LIMATGQKPGALVEALLVLKARNLNMSKLESRPIPGTPWEEMFYLDIDANLSSEPMQAAL
EEEECCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHEEEEEECCCCCCCHHHHHH
KELERTTRFIKVLGCYPCETVKPTQLSNSQLMIEPDTSKSTTVTATPPNKQMRVSKQYKS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCHHHHHHHHCC
ESTQVICGQLSLGNGNLGAIAQVQLPLDLQQFEQMAKELKESGFQAVVIQGLSQQSDLIS
CCCEEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEHHCCCHHHHHHH
SIEKFKHALGQFNLVCVLAIEHETDLSMAIQFADVVMLAGNLMYNQAILSQLGSLLVPVI
HHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERNAMASVDDLLNAAEEVLSQGNQQLILCESGVTTLNNSGKPSLDLAALVELKALSHLP
HCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCC
VVVNPSYAIDTDQLATFAKAIKQLKGDGVMMNLSAIDTAAQAHPEELVKAVLGNLYH
EEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]