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The map label for this gene is pyrB [H]

Identifier: 157960803

GI number: 157960803

Start: 1186922

End: 1187941

Strand: Reverse

Name: pyrB [H]

Synonym: Spea_0975

Alternate gene names: 157960803

Gene position: 1187941-1186922 (Counterclockwise)

Preceding gene: 157960805

Following gene: 157960802

Centisome position: 22.96

GC content: 45.39

Gene sequence:

>1020_bases
ATGACTCAGTTTCAAGGTTCGCACATCCTTTCGGTAAACCAACTAGACTTGGAAGCCGTTGAAAAAGTTTTTTCTGTTGC
TACTCGCATGACTCCCTATGCTTTACGACAAAAACGTACCACTGTGTTAGATGGTGCCGTTTTGGGCAATCTGTTTTTCG
AACCAAGTACCCGTACACGTGTCAGCTTTGCTAGCGCCTTTAACCTACTCGGCGGTAGAGTTAACGAAACCGTGGGTATG
GCGTCTTCGTCATTGTCTAAGGGCGAATCGCTATACGATACCGCTCGCGTTTTATCTAGTTACTCAGATGTGATTGCCAT
GCGTCACCCAGATTCTTTCTCTGTGAGTGAATTTGCTAAAGGCAGCCGCGTACCGGTAATTAACGGCGGTGATGGTTCAA
ACGAGCACCCTACTCAAGCGCTACTGGATTTATTTACCATTCAAAAAGAGCTAGCGGCTGGCGGCCGTGATATCAGTGGT
ATGAATATCGCCATGGTCGGCGATTTGAAATTTGGCCGCACCGTACACTCGCTTTCGCGTCTGCTATGCATGTATAAAGA
TGTCAGCTTCACACTCGTATCACCAAAAGAGCTAGCAATGCCTGACTCTGTGATCAATGACATTGCAAATGCTGGCCATA
AGATCACAATAACAGATCAACTTGAAGGCAATTTAGATCGCGCAGATATACTCTACCTGACGCGTATTCAAGAAGAACGC
TTCCCTTCTCAAGAAGAAGCGAACAAATACCGCGGCAAGTTCCGTTTAAACCAAAGTATTTATACACAGCATTGCAAATC
AAACACTGTGATCATGCATCCATTGCCACGAGACTCACGTGAGCAAGCAAATGAATTGGATAACGATCTTAACGACCATC
CAAATCTGGCTATCTTTAGACAAGCCGATAATGGTCTGTTGATCCGCATGGCGCTGTTTGCACTGACGTTAGGTGTTGAC
TCTCAGCTTGAGCAATACGAACGTCCAGTTAATTGGTATTCACGAAAAGCTGATGTGTAA

Upstream 100 bases:

>100_bases
CTGCCAATTAGATTCTGCTATAGTGCCGCCTCAGCCACCCTACATAAATTTGGGCTATAGCCATGCTATATCGCCCCTCA
GAAATTGGAAATATAGGCTG

Downstream 100 bases:

>100_bases
ATCGGCTATAGACTTTAAGGATTAAAAATGACTCGTTCTGACGAACTATTTGAACAGGCTAAAAAGACCATCCCAGGTGG
CGTTAACTCTCCAGTACGTG

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTQFQGSHILSVNQLDLEAVEKVFSVATRMTPYALRQKRTTVLDGAVLGNLFFEPSTRTRVSFASAFNLLGGRVNETVGM
ASSSLSKGESLYDTARVLSSYSDVIAMRHPDSFSVSEFAKGSRVPVINGGDGSNEHPTQALLDLFTIQKELAAGGRDISG
MNIAMVGDLKFGRTVHSLSRLLCMYKDVSFTLVSPKELAMPDSVINDIANAGHKITITDQLEGNLDRADILYLTRIQEER
FPSQEEANKYRGKFRLNQSIYTQHCKSNTVIMHPLPRDSREQANELDNDLNDHPNLAIFRQADNGLLIRMALFALTLGVD
SQLEQYERPVNWYSRKADV

Sequences:

>Translated_339_residues
MTQFQGSHILSVNQLDLEAVEKVFSVATRMTPYALRQKRTTVLDGAVLGNLFFEPSTRTRVSFASAFNLLGGRVNETVGM
ASSSLSKGESLYDTARVLSSYSDVIAMRHPDSFSVSEFAKGSRVPVINGGDGSNEHPTQALLDLFTIQKELAAGGRDISG
MNIAMVGDLKFGRTVHSLSRLLCMYKDVSFTLVSPKELAMPDSVINDIANAGHKITITDQLEGNLDRADILYLTRIQEER
FPSQEEANKYRGKFRLNQSIYTQHCKSNTVIMHPLPRDSREQANELDNDLNDHPNLAIFRQADNGLLIRMALFALTLGVD
SQLEQYERPVNWYSRKADV
>Mature_338_residues
TQFQGSHILSVNQLDLEAVEKVFSVATRMTPYALRQKRTTVLDGAVLGNLFFEPSTRTRVSFASAFNLLGGRVNETVGMA
SSSLSKGESLYDTARVLSSYSDVIAMRHPDSFSVSEFAKGSRVPVINGGDGSNEHPTQALLDLFTIQKELAAGGRDISGM
NIAMVGDLKFGRTVHSLSRLLCMYKDVSFTLVSPKELAMPDSVINDIANAGHKITITDQLEGNLDRADILYLTRIQEERF
PSQEEANKYRGKFRLNQSIYTQHCKSNTVIMHPLPRDSREQANELDNDLNDHPNLAIFRQADNGLLIRMALFALTLGVDS
QLEQYERPVNWYSRKADV

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=313, Percent_Identity=45.3674121405751, Blast_Score=252, Evalue=3e-67,
Organism=Homo sapiens, GI38788445, Length=321, Percent_Identity=26.791277258567, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI2367364, Length=317, Percent_Identity=39.4321766561514, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1786469, Length=343, Percent_Identity=26.2390670553936, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790703, Length=240, Percent_Identity=29.1666666666667, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=333, Percent_Identity=40.2402402402402, Blast_Score=224, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6322331, Length=321, Percent_Identity=43.613707165109, Blast_Score=248, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6322373, Length=279, Percent_Identity=25.8064516129032, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24642586, Length=316, Percent_Identity=41.4556962025316, Blast_Score=238, Evalue=4e-63,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 37739; Mature: 37608

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQFQGSHILSVNQLDLEAVEKVFSVATRMTPYALRQKRTTVLDGAVLGNLFFEPSTRTR
CCCCCCCEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEECCCCCHH
VSFASAFNLLGGRVNETVGMASSSLSKGESLYDTARVLSSYSDVIAMRHPDSFSVSEFAK
HHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHCC
GSRVPVINGGDGSNEHPTQALLDLFTIQKELAAGGRDISGMNIAMVGDLKFGRTVHSLSR
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHH
LLCMYKDVSFTLVSPKELAMPDSVINDIANAGHKITITDQLEGNLDRADILYLTRIQEER
HHHHHCCCEEEEECCHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHEEEHHHHHH
FPSQEEANKYRGKFRLNQSIYTQHCKSNTVIMHPLPRDSREQANELDNDLNDHPNLAIFR
CCCHHHHHHHCCCEEECHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCEEEEE
QADNGLLIRMALFALTLGVDSQLEQYERPVNWYSRKADV
ECCCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHCCCC
>Mature Secondary Structure 
TQFQGSHILSVNQLDLEAVEKVFSVATRMTPYALRQKRTTVLDGAVLGNLFFEPSTRTR
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEECCCCCHH
VSFASAFNLLGGRVNETVGMASSSLSKGESLYDTARVLSSYSDVIAMRHPDSFSVSEFAK
HHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHCC
GSRVPVINGGDGSNEHPTQALLDLFTIQKELAAGGRDISGMNIAMVGDLKFGRTVHSLSR
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHH
LLCMYKDVSFTLVSPKELAMPDSVINDIANAGHKITITDQLEGNLDRADILYLTRIQEER
HHHHHCCCEEEEECCHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHEEEHHHHHH
FPSQEEANKYRGKFRLNQSIYTQHCKSNTVIMHPLPRDSREQANELDNDLNDHPNLAIFR
CCCHHHHHHHCCCEEECHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCEEEEE
QADNGLLIRMALFALTLGVDSQLEQYERPVNWYSRKADV
ECCCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA