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The map label for this gene is rhtB [H]

Identifier: 157960643

GI number: 157960643

Start: 1012326

End: 1012940

Strand: Direct

Name: rhtB [H]

Synonym: Spea_0815

Alternate gene names: 157960643

Gene position: 1012326-1012940 (Clockwise)

Preceding gene: 157960640

Following gene: 157960645

Centisome position: 19.56

GC content: 48.94

Gene sequence:

>615_bases
ATGGCATTAGACACTTGGCTTATTTATCTCGTTGCTGTTATCGGTCTGTCGCTCGCCCCCGGGCCCAATGGACTGCTGGC
CTTAACTCACGGGGTAATGTATGGGCATAGAAAGACACTTTTTACCATTAGCGGCGGTGTGCTGGGTTTTGTTGTGTTGA
TCGCTCTGGCTATGTTTGGCATCGGTGCATTACTGCTTGCCTCAACGGATCTGCTATTAGTCTTTAAGTGGATAGGCGGC
ATATACCTTATTTGGCTGGGTATACGCATTTGGCGCTCCCCTGCAATAGCGTTGCAGCCCGTAGGTAAGGCGGTGGCTGT
GAGTCAGTTTGGGCTTTTTAGACAAGGCGCGTTAGCGGCCATAACCAACCCTAAGGTGCTGTTATTTTTTGGTGCCTTCT
TGCCTCAATTTATCGATCCAGAAAAGTCATTACTCAGCCAGTTTATGGTGATGGCTGCGACATTCGCGGTGGTCGAATTT
GTTGTCGAATACTTGATCGCCAGAGTCGCGACTAAACTTAGACCATGGCTTGCTCGCAGTGGTAAGCGCTTCAATCAATG
TTGTGGTGGAATGTTCGCGCTGATTGGCGCCAGCTTACCCTTGTACAATAATTAA

Upstream 100 bases:

>100_bases
ATATTACCTTGCTTTGTCTTGATTGTATCAGGTTCGATAGTTCCTCCTCTCCTATAATCCGCTCCAACATAGAAACTATC
GCTTTATCACAGGAAAAAGT

Downstream 100 bases:

>100_bases
ATCAGGGTTTCCTTGGCTCGGTATAGAAGGGGATCGCAGTGGCTTATTGGATTGTTATGAGTGGCTTTTTCGAAGAGCTA
ATGGCTTATCTAAATGTGAG

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MALDTWLIYLVAVIGLSLAPGPNGLLALTHGVMYGHRKTLFTISGGVLGFVVLIALAMFGIGALLLASTDLLLVFKWIGG
IYLIWLGIRIWRSPAIALQPVGKAVAVSQFGLFRQGALAAITNPKVLLFFGAFLPQFIDPEKSLLSQFMVMAATFAVVEF
VVEYLIARVATKLRPWLARSGKRFNQCCGGMFALIGASLPLYNN

Sequences:

>Translated_204_residues
MALDTWLIYLVAVIGLSLAPGPNGLLALTHGVMYGHRKTLFTISGGVLGFVVLIALAMFGIGALLLASTDLLLVFKWIGG
IYLIWLGIRIWRSPAIALQPVGKAVAVSQFGLFRQGALAAITNPKVLLFFGAFLPQFIDPEKSLLSQFMVMAATFAVVEF
VVEYLIARVATKLRPWLARSGKRFNQCCGGMFALIGASLPLYNN
>Mature_203_residues
ALDTWLIYLVAVIGLSLAPGPNGLLALTHGVMYGHRKTLFTISGGVLGFVVLIALAMFGIGALLLASTDLLLVFKWIGGI
YLIWLGIRIWRSPAIALQPVGKAVAVSQFGLFRQGALAAITNPKVLLFFGAFLPQFIDPEKSLLSQFMVMAATFAVVEFV
VEYLIARVATKLRPWLARSGKRFNQCCGGMFALIGASLPLYNN

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=202, Percent_Identity=35.6435643564356, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI1788099, Length=161, Percent_Identity=31.055900621118, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22060; Mature: 21929

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDTWLIYLVAVIGLSLAPGPNGLLALTHGVMYGHRKTLFTISGGVLGFVVLIALAMFG
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHH
IGALLLASTDLLLVFKWIGGIYLIWLGIRIWRSPAIALQPVGKAVAVSQFGLFRQGALAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCCE
ITNPKVLLFFGAFLPQFIDPEKSLLSQFMVMAATFAVVEFVVEYLIARVATKLRPWLARS
ECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKRFNQCCGGMFALIGASLPLYNN
CCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ALDTWLIYLVAVIGLSLAPGPNGLLALTHGVMYGHRKTLFTISGGVLGFVVLIALAMFG
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHH
IGALLLASTDLLLVFKWIGGIYLIWLGIRIWRSPAIALQPVGKAVAVSQFGLFRQGALAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCCE
ITNPKVLLFFGAFLPQFIDPEKSLLSQFMVMAATFAVVEFVVEYLIARVATKLRPWLARS
ECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKRFNQCCGGMFALIGASLPLYNN
CCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]