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The map label for this gene is braB [H]

Identifier: 157960632

GI number: 157960632

Start: 996241

End: 997587

Strand: Direct

Name: braB [H]

Synonym: Spea_0804

Alternate gene names: 157960632

Gene position: 996241-997587 (Clockwise)

Preceding gene: 157960627

Following gene: 157960633

Centisome position: 19.25

GC content: 44.54

Gene sequence:

>1347_bases
ATGATGGGGCATAAAGTGCAGAATAATCAAATGAGTATTGCAGACACGCTAGGGTTAGGTTTTATGACCTTTGCGTTTTT
TTTGGGGGCTGGTAATTTAATCTTCCCTCCTTTAGCTGGCTATTTGGCAGGCGAAAATATCACGCTAGCTATGTTTGGCT
TTCTTATTACAGCGGTCGGTCTTCCCCTTATTACTCTGATTGCTGTCGCTAAGGCGAACGGTAAAATTATGCAGTTGCTG
CCTCCCATCGCAGCGACCGCATTAGCAGTGTCAATTTTTATCATCATAGGTCCAGCGTTCGCCGCACCAAGAACCGGACT
CGTGGCTTATGAAGTCGGCTTTAAGCCATTTTTAGAAGATACATCAGCCACCTTTATGATAGCTGGTTTGACCTTTAACA
TAACTCAGCTTATTTATACGTTGGGCTTCTTTGGTCTTTCAATGGTGTTAGCGCTGTTCCCGGGTAAGTTGTTGGATAGC
GTCGGTAAAGTATTAACCCCTATTCTGATCCTGTTGTTAGTAGGTCTAGCGATCTCTGTTATCGTGATCCCTGGTAGCGA
TATTGGTGCTGCGGTTGGCGATTATCAAGCAAATCCACTTACTAAGGGGATCTTGGAAGGCTATAACACCATGGATACCT
TGGCTTCATTGATCTTCGGTATGTTGATTATCGATATCCTGCGCAAGAAAGGTATCACTGAGCCAAAAGCACAGACTAAC
TATCTGATTAAAGCTGCACTAATTGCTGCGGCAGGCTTGGCATTTGTTTATGTGTCTCTATTTTACTTAGGCGCAACGGC
GGGGGATTTAGCTGTTGGTGCAGAAAACGGCGGCGTGATCTTAACTAATTACGTTATCGCTAAATTTGGCGCAAGCGGCA
TGGTATTGCTTTCATCTGTAGTAACACTAGCCTGTTTAACCACTGCGGTAGGCCTTATTAGTGCCTGTAGTGAGTACTTT
AACGAGCTATTGCCAAAGATCTCTTACCGTCGTTTTGTTGTATTGGTGAGTGTTGTTTGTGCAACCGTTGCTAACGTTGG
TTTAGCGCAGCTAATCGACATCAGCGTGCCTGTACTCATGACTGTTTACCCTGTGGCGATCGCATTGGTTGCGGTGACCT
TCTTAACTGAACGTTTTGCTCACCCAAAAACAGCGCATCGCTTAGTTGTTAGTGTGGCGCTATTCTTCGGTATTATTGAT
GGCTTTAAAGCGGCTAAAGTTGATGTAAGCATGTTTAACTTTATGCCACTACATAACGAAGGCATGGCATGGTTGCTACC
AACAGCTATCGTGATTGTGGCTTGTATGCTGATTAAAAAGCCTAACCAGCAGCTAGCGGCAAACTAA

Upstream 100 bases:

>100_bases
CCACGGAACAGTGTAAAGTAATAATTACACCTTTTTTTGTTGTCATCCTAAGTGGTGCCTTAGGAATATGACGGCATGAA
ATATTTAACCAAATAAAATT

Downstream 100 bases:

>100_bases
AGCGAGCGGCAAACTAAAGCGAGCGGTTAAAGCTAATAGCTAGTGTGAATAGCTAAAGCCAGTTTGATGGCATAATATTG
AGGCGATGTTTCTAGGAACA

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MMGHKVQNNQMSIADTLGLGFMTFAFFLGAGNLIFPPLAGYLAGENITLAMFGFLITAVGLPLITLIAVAKANGKIMQLL
PPIAATALAVSIFIIIGPAFAAPRTGLVAYEVGFKPFLEDTSATFMIAGLTFNITQLIYTLGFFGLSMVLALFPGKLLDS
VGKVLTPILILLLVGLAISVIVIPGSDIGAAVGDYQANPLTKGILEGYNTMDTLASLIFGMLIIDILRKKGITEPKAQTN
YLIKAALIAAAGLAFVYVSLFYLGATAGDLAVGAENGGVILTNYVIAKFGASGMVLLSSVVTLACLTTAVGLISACSEYF
NELLPKISYRRFVVLVSVVCATVANVGLAQLIDISVPVLMTVYPVAIALVAVTFLTERFAHPKTAHRLVVSVALFFGIID
GFKAAKVDVSMFNFMPLHNEGMAWLLPTAIVIVACMLIKKPNQQLAAN

Sequences:

>Translated_448_residues
MMGHKVQNNQMSIADTLGLGFMTFAFFLGAGNLIFPPLAGYLAGENITLAMFGFLITAVGLPLITLIAVAKANGKIMQLL
PPIAATALAVSIFIIIGPAFAAPRTGLVAYEVGFKPFLEDTSATFMIAGLTFNITQLIYTLGFFGLSMVLALFPGKLLDS
VGKVLTPILILLLVGLAISVIVIPGSDIGAAVGDYQANPLTKGILEGYNTMDTLASLIFGMLIIDILRKKGITEPKAQTN
YLIKAALIAAAGLAFVYVSLFYLGATAGDLAVGAENGGVILTNYVIAKFGASGMVLLSSVVTLACLTTAVGLISACSEYF
NELLPKISYRRFVVLVSVVCATVANVGLAQLIDISVPVLMTVYPVAIALVAVTFLTERFAHPKTAHRLVVSVALFFGIID
GFKAAKVDVSMFNFMPLHNEGMAWLLPTAIVIVACMLIKKPNQQLAAN
>Mature_448_residues
MMGHKVQNNQMSIADTLGLGFMTFAFFLGAGNLIFPPLAGYLAGENITLAMFGFLITAVGLPLITLIAVAKANGKIMQLL
PPIAATALAVSIFIIIGPAFAAPRTGLVAYEVGFKPFLEDTSATFMIAGLTFNITQLIYTLGFFGLSMVLALFPGKLLDS
VGKVLTPILILLLVGLAISVIVIPGSDIGAAVGDYQANPLTKGILEGYNTMDTLASLIFGMLIIDILRKKGITEPKAQTN
YLIKAALIAAAGLAFVYVSLFYLGATAGDLAVGAENGGVILTNYVIAKFGASGMVLLSSVVTLACLTTAVGLISACSEYF
NELLPKISYRRFVVLVSVVCATVANVGLAQLIDISVPVLMTVYPVAIALVAVTFLTERFAHPKTAHRLVVSVALFFGIID
GFKAAKVDVSMFNFMPLHNEGMAWLLPTAIVIVACMLIKKPNQQLAAN

Specific function: Component of the LIV-II transport system for branched- chain amino acids. BraB is specific for isoleucine, leucine and valine. The LIV-II transport system is coupled to sodium and lithium ions [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=442, Percent_Identity=41.1764705882353, Blast_Score=328, Evalue=6e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 47194; Mature: 47194

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMGHKVQNNQMSIADTLGLGFMTFAFFLGAGNLIFPPLAGYLAGENITLAMFGFLITAVG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHH
LPLITLIAVAKANGKIMQLLPPIAATALAVSIFIIIGPAFAAPRTGLVAYEVGFKPFLED
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHCC
TSATFMIAGLTFNITQLIYTLGFFGLSMVLALFPGKLLDSVGKVLTPILILLLVGLAISV
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEE
IVIPGSDIGAAVGDYQANPLTKGILEGYNTMDTLASLIFGMLIIDILRKKGITEPKAQTN
EEECCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
YLIKAALIAAAGLAFVYVSLFYLGATAGDLAVGAENGGVILTNYVIAKFGASGMVLLSSV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEHHHHHHHCCCHHHHHHHH
VTLACLTTAVGLISACSEYFNELLPKISYRRFVVLVSVVCATVANVGLAQLIDISVPVLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TVYPVAIALVAVTFLTERFAHPKTAHRLVVSVALFFGIIDGFKAAKVDVSMFNFMPLHNE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHEEEHHHHHEEEECCC
GMAWLLPTAIVIVACMLIKKPNQQLAAN
CCHHHHHHHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure
MMGHKVQNNQMSIADTLGLGFMTFAFFLGAGNLIFPPLAGYLAGENITLAMFGFLITAVG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHH
LPLITLIAVAKANGKIMQLLPPIAATALAVSIFIIIGPAFAAPRTGLVAYEVGFKPFLED
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHCC
TSATFMIAGLTFNITQLIYTLGFFGLSMVLALFPGKLLDSVGKVLTPILILLLVGLAISV
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEE
IVIPGSDIGAAVGDYQANPLTKGILEGYNTMDTLASLIFGMLIIDILRKKGITEPKAQTN
EEECCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
YLIKAALIAAAGLAFVYVSLFYLGATAGDLAVGAENGGVILTNYVIAKFGASGMVLLSSV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEHHHHHHHCCCHHHHHHHH
VTLACLTTAVGLISACSEYFNELLPKISYRRFVVLVSVVCATVANVGLAQLIDISVPVLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TVYPVAIALVAVTFLTERFAHPKTAHRLVVSVALFFGIIDGFKAAKVDVSMFNFMPLHNE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHEEEHHHHHEEEECCC
GMAWLLPTAIVIVACMLIKKPNQQLAAN
CCHHHHHHHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2111004; 10984043 [H]