Click here to switch to the map view.

The map label for this gene is prfB [H]

Identifier: 157960584

GI number: 157960584

Start: 938835

End: 939716

Strand: Direct

Name: prfB [H]

Synonym: Spea_0756

Alternate gene names: 157960584

Gene position: 938835-939716 (Clockwise)

Preceding gene: 157960581

Following gene: 157960585

Centisome position: 18.14

GC content: 47.39

Gene sequence:

>882_bases
ATGGACAGTGGCCTAGAAGATATCGAAGGCTTACTAGAGCTAGCCGTTGAAGAGAACGATGAAGAGACCTTTAACGACGC
CGCTGCGGAATTACAGGGCTTAGAGAAACGCCTTGAGGAACTTGAGTTCCGTCGTATGTTCTCTAAACCACATGATGCTT
CAGATTGTTATTTAGATATTCAATCAGGATCGGGCGGAACAGAAGCGCAAGATTGGGCCAATATGGTGCTGCGTATGTTC
TTACGTTGGGGTGAAGCCCACGACTATAAGCCGGAACTGATTGAAGTGACTGCGGGTGATGTTGCCGGTATTAAAGGCGC
AACCATTAAGTTTACCGGTGAATATGCTTTTGGTTCACTGCGCACCGAAACCGGTGTACACCGTTTAGTGCGTAAGTCGC
CATTTGACTCGTCGGGTAAGCGTCACACCTCTTTCTGTTCGGTATTCGTTTACCCAGAAATTGATGATTCGATTGAGATT
GATATCAATCCTTCGGACTTAAGAATTGATACCTACCGCGCGTCAGGTGCGGGTGGTCAGCACGTTAACAAAACTGAGTC
GGCAATTCGTATTACCCATTTACCGACCAATACCGTTGTGCAGTGTCAGAACGACCGTTCGCAGCATAAGAACCGTGATG
CTGCGATGAAACAGCTGAAGGCAAAGCTGTATGAGCTAGAAATGCTCAAGCAGAATGCTGATAAGCAAGCCGCCGAAGAT
GCCAAGTCTGATATTGGCTGGGGCAGCCAGATCCGTTCATACGTGCTCGATGATGCACGTATTAAAGATCTACGTACCGG
CGTTGAAAACCGAAATACCCAGAGCGTTTTAGATGGCGATCTGGACAAGTTTATTGAAGCTAGCCTGAAATCTGGCCTTT
AA

Upstream 100 bases:

>100_bases
AGAGCTTGAAAGCTCTGAAGTTTGGAATGATCCAGAAAACGCCCAAGCCCTAGGCAAGGAGCGTGCAGCACTTGAAGCGG
TAGTGAAGACTATCGACGAT

Downstream 100 bases:

>100_bases
CGAGAGAAGAAGATGACTGAACAAACCCAAGACGAGAACAAGTTAATTGCAGAGCGTCGTGCTAAATTAGAGCACATTCG
TACCAACTGCCCAGCAAACG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDSGLEDIEGLLELAVEENDEETFNDAAAELQGLEKRLEELEFRRMFSKPHDASDCYLDIQSGSGGTEAQDWANMVLRMF
LRWGEAHDYKPELIEVTAGDVAGIKGATIKFTGEYAFGSLRTETGVHRLVRKSPFDSSGKRHTSFCSVFVYPEIDDSIEI
DINPSDLRIDTYRASGAGGQHVNKTESAIRITHLPTNTVVQCQNDRSQHKNRDAAMKQLKAKLYELEMLKQNADKQAAED
AKSDIGWGSQIRSYVLDDARIKDLRTGVENRNTQSVLDGDLDKFIEASLKSGL

Sequences:

>Translated_293_residues
MDSGLEDIEGLLELAVEENDEETFNDAAAELQGLEKRLEELEFRRMFSKPHDASDCYLDIQSGSGGTEAQDWANMVLRMF
LRWGEAHDYKPELIEVTAGDVAGIKGATIKFTGEYAFGSLRTETGVHRLVRKSPFDSSGKRHTSFCSVFVYPEIDDSIEI
DINPSDLRIDTYRASGAGGQHVNKTESAIRITHLPTNTVVQCQNDRSQHKNRDAAMKQLKAKLYELEMLKQNADKQAAED
AKSDIGWGSQIRSYVLDDARIKDLRTGVENRNTQSVLDGDLDKFIEASLKSGL
>Mature_293_residues
MDSGLEDIEGLLELAVEENDEETFNDAAAELQGLEKRLEELEFRRMFSKPHDASDCYLDIQSGSGGTEAQDWANMVLRMF
LRWGEAHDYKPELIEVTAGDVAGIKGATIKFTGEYAFGSLRTETGVHRLVRKSPFDSSGKRHTSFCSVFVYPEIDDSIEI
DINPSDLRIDTYRASGAGGQHVNKTESAIRITHLPTNTVVQCQNDRSQHKNRDAAMKQLKAKLYELEMLKQNADKQAAED
AKSDIGWGSQIRSYVLDDARIKDLRTGVENRNTQSVLDGDLDKFIEASLKSGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=239, Percent_Identity=35.5648535564854, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI34577120, Length=306, Percent_Identity=32.3529411764706, Blast_Score=157, Evalue=8e-39,
Organism=Homo sapiens, GI166795305, Length=146, Percent_Identity=39.041095890411, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI2367172, Length=293, Percent_Identity=75.0853242320819, Blast_Score=475, Evalue=1e-135,
Organism=Escherichia coli, GI1787462, Length=244, Percent_Identity=40.5737704918033, Blast_Score=193, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=241, Percent_Identity=31.1203319502075, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=250, Percent_Identity=35.2, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19921226, Length=207, Percent_Identity=40.0966183574879, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 32740; Mature: 32740

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSGLEDIEGLLELAVEENDEETFNDAAAELQGLEKRLEELEFRRMFSKPHDASDCYLDI
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
QSGSGGTEAQDWANMVLRMFLRWGEAHDYKPELIEVTAGDVAGIKGATIKFTGEYAFGSL
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCH
RTETGVHRLVRKSPFDSSGKRHTSFCSVFVYPEIDDSIEIDINPSDLRIDTYRASGAGGQ
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCEEEEEEECCCCCCC
HVNKTESAIRITHLPTNTVVQCQNDRSQHKNRDAAMKQLKAKLYELEMLKQNADKQAAED
CCCCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AKSDIGWGSQIRSYVLDDARIKDLRTGVENRNTQSVLDGDLDKFIEASLKSGL
HHHCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDSGLEDIEGLLELAVEENDEETFNDAAAELQGLEKRLEELEFRRMFSKPHDASDCYLDI
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
QSGSGGTEAQDWANMVLRMFLRWGEAHDYKPELIEVTAGDVAGIKGATIKFTGEYAFGSL
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCH
RTETGVHRLVRKSPFDSSGKRHTSFCSVFVYPEIDDSIEIDINPSDLRIDTYRASGAGGQ
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCEEEEEEECCCCCCC
HVNKTESAIRITHLPTNTVVQCQNDRSQHKNRDAAMKQLKAKLYELEMLKQNADKQAAED
CCCCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AKSDIGWGSQIRSYVLDDARIKDLRTGVENRNTQSVLDGDLDKFIEASLKSGL
HHHCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA