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The map label for this gene is lysS [H]

Identifier: 157960585

GI number: 157960585

Start: 939729

End: 941234

Strand: Direct

Name: lysS [H]

Synonym: Spea_0757

Alternate gene names: 157960585

Gene position: 939729-941234 (Clockwise)

Preceding gene: 157960584

Following gene: 157960586

Centisome position: 18.16

GC content: 46.88

Gene sequence:

>1506_bases
ATGACTGAACAAACCCAAGACGAGAACAAGTTAATTGCAGAGCGTCGTGCTAAATTAGAGCACATTCGTACCAACTGCCC
AGCAAACGGTCACCCGAACAACTTCGATCGAAAACACAAAGCGGCAGATATCCAAGCTGAATTTGGTCACAACACCAAAG
AAGAGCTTGAAGGCATGGGCATTGAGCGTTCAATCGCTGGCCGTGTTATGGCGAAGCGTGGTCCATTCTTGGTGATCCAA
GATGTGAGCGGTCGTATCCAAGCTTATGCGGGTAAAGACGTTCAAAAAGATCTTAAAGAGAAGTTCTTAGGTCTAGATAT
TGGTGACATCATAGGTGTGACCGGTCAGCTACACCTTTCAGGTAAGGGCGACCTATACGTGAACATGGAAGAGTACCAGT
TGCTAACTAAGGCACTGCGTCCACTTCCTGAAAAGTTTCACGGTCTAACTGACCAAGAGACTCGCTACCGTCAGCGTTAT
GTAGACTTGATCGTTAACGAAGACTCTCGTGAAGCCTTTATCATGCGTTCTAAAGTGGTTTCGGCTATCCGTAACTTCAT
GGTGAAGAAAGAGTTCATGGAAGTTGAAACACCTATGATGCACAGCATTCCAGGTGGTGCTTCAGCACGTCCATTCGAGA
CGCATCATAATGCTCTTGATATCGCTATGTACCTACGTATTGCACCTGAGCTATACCTTAAGCGTCTAGTGGTTGGTGGT
TTTGAGCGCGTATTCGAAATCAACCGTAACTTCCGTAACGAAGGTCTGTCTCCTCGCCATAACCCTGAATTCACTATGAT
GGAATTCTATATGGCGTACGCTGACTTCAACGATCTAATGGATCTGACTGAAGAGATGCTAAGCTCTATCGCAACTGAGC
TATGTGGTTCTCCACAGCTTCCATACGGTGAGCACACTGTTGATTTTGGTGGCCCATATGCACGTTTGAGCATGTTGGAT
GCAATCAAGAAGTACAACCCAGACAACGCAACAATCCAGTCTATGACTTATGAAGAAGTTAAAGACGTTGAGTTTATGCG
TGACTTGGCTAAGAGCCTAGGCATGACAGTTGAGAAGTTCTGGACTTGTGGCCAGCTACTAGAAGAGATCTTTGGTGAAA
CAGCTGAAACTCAGTTGATGCAACCTACCTTCATCACTGGCTACCCAGCGGATATCTCTCCACTAGCACGTCGTAACGAC
GACAACCATTTCATCACAGACCGTTTCGAGTTCTTCATCGGCGGCCGTGAAGTGGCTAACGGTTTCTCTGAGCTTAACGA
TGCTGAAGATCAAGACAGCCGTTTCAAGGCTCAGGTTGATGCTAAAGATGCTGGTGATGACGAAGCGATGTTCTATGACG
CTGACTACATCACTGCACTAGAGCACGGCTTACCACCAACTGCGGGTCAAGGTATCGGTATCGACCGTCTAGTGATGCTA
TTCACTAACACGCACACTATCCGTGACGTGATCCTATTCCCTGCGATGCGTCCACAAGCTAACTAA

Upstream 100 bases:

>100_bases
TACCGGCGTTGAAAACCGAAATACCCAGAGCGTTTTAGATGGCGATCTGGACAAGTTTATTGAAGCTAGCCTGAAATCTG
GCCTTTAACGAGAGAAGAAG

Downstream 100 bases:

>100_bases
TCCTTAGCATATAGCTGCATAGCAGTAAGACTTTAAAAACCAGCCACTAGGCTGGTTTTTTTATGTCACAATTTCAAATT
TTGCATTTCTGTGACCAATT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MTEQTQDENKLIAERRAKLEHIRTNCPANGHPNNFDRKHKAADIQAEFGHNTKEELEGMGIERSIAGRVMAKRGPFLVIQ
DVSGRIQAYAGKDVQKDLKEKFLGLDIGDIIGVTGQLHLSGKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRY
VDLIVNEDSREAFIMRSKVVSAIRNFMVKKEFMEVETPMMHSIPGGASARPFETHHNALDIAMYLRIAPELYLKRLVVGG
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADFNDLMDLTEEMLSSIATELCGSPQLPYGEHTVDFGGPYARLSMLD
AIKKYNPDNATIQSMTYEEVKDVEFMRDLAKSLGMTVEKFWTCGQLLEEIFGETAETQLMQPTFITGYPADISPLARRND
DNHFITDRFEFFIGGREVANGFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML
FTNTHTIRDVILFPAMRPQAN

Sequences:

>Translated_501_residues
MTEQTQDENKLIAERRAKLEHIRTNCPANGHPNNFDRKHKAADIQAEFGHNTKEELEGMGIERSIAGRVMAKRGPFLVIQ
DVSGRIQAYAGKDVQKDLKEKFLGLDIGDIIGVTGQLHLSGKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRY
VDLIVNEDSREAFIMRSKVVSAIRNFMVKKEFMEVETPMMHSIPGGASARPFETHHNALDIAMYLRIAPELYLKRLVVGG
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADFNDLMDLTEEMLSSIATELCGSPQLPYGEHTVDFGGPYARLSMLD
AIKKYNPDNATIQSMTYEEVKDVEFMRDLAKSLGMTVEKFWTCGQLLEEIFGETAETQLMQPTFITGYPADISPLARRND
DNHFITDRFEFFIGGREVANGFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML
FTNTHTIRDVILFPAMRPQAN
>Mature_500_residues
TEQTQDENKLIAERRAKLEHIRTNCPANGHPNNFDRKHKAADIQAEFGHNTKEELEGMGIERSIAGRVMAKRGPFLVIQD
VSGRIQAYAGKDVQKDLKEKFLGLDIGDIIGVTGQLHLSGKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRYV
DLIVNEDSREAFIMRSKVVSAIRNFMVKKEFMEVETPMMHSIPGGASARPFETHHNALDIAMYLRIAPELYLKRLVVGGF
ERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADFNDLMDLTEEMLSSIATELCGSPQLPYGEHTVDFGGPYARLSMLDA
IKKYNPDNATIQSMTYEEVKDVEFMRDLAKSLGMTVEKFWTCGQLLEEIFGETAETQLMQPTFITGYPADISPLARRNDD
NHFITDRFEFFIGGREVANGFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVMLF
TNTHTIRDVILFPAMRPQAN

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=518, Percent_Identity=40.9266409266409, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI194272210, Length=518, Percent_Identity=40.9266409266409, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI45439306, Length=351, Percent_Identity=24.5014245014245, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI40789249, Length=254, Percent_Identity=25.5905511811024, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1790571, Length=499, Percent_Identity=60.7214428857716, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI1789256, Length=498, Percent_Identity=60.4417670682731, Blast_Score=610, Evalue=1e-176,
Organism=Escherichia coli, GI87082379, Length=330, Percent_Identity=30.9090909090909, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1788173, Length=323, Percent_Identity=24.1486068111455, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535927, Length=509, Percent_Identity=39.8821218074656, Blast_Score=357, Evalue=8e-99,
Organism=Caenorhabditis elegans, GI17535925, Length=509, Percent_Identity=39.8821218074656, Blast_Score=357, Evalue=9e-99,
Organism=Caenorhabditis elegans, GI71994340, Length=509, Percent_Identity=39.8821218074656, Blast_Score=356, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI17551876, Length=356, Percent_Identity=26.685393258427, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32566633, Length=278, Percent_Identity=28.0575539568345, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320242, Length=518, Percent_Identity=41.1196911196911, Blast_Score=373, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324256, Length=398, Percent_Identity=30.4020100502513, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6323011, Length=356, Percent_Identity=25.8426966292135, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6325153, Length=305, Percent_Identity=26.2295081967213, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24640851, Length=512, Percent_Identity=40.234375, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640849, Length=512, Percent_Identity=40.234375, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI17136276, Length=461, Percent_Identity=22.9934924078091, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24584738, Length=333, Percent_Identity=24.6246246246246, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56963; Mature: 56832

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQTQDENKLIAERRAKLEHIRTNCPANGHPNNFDRKHKAADIQAEFGHNTKEELEGMG
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCHHHCCCCCHHHHHCCC
IERSIAGRVMAKRGPFLVIQDVSGRIQAYAGKDVQKDLKEKFLGLDIGDIIGVTGQLHLS
CCHHHHHHHHHCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHCCCHHHHHCCCCEEEEC
GKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRYVDLIVNEDSREAFIMRSKVV
CCCCEEECHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SAIRNFMVKKEFMEVETPMMHSIPGGASARPFETHHNALDIAMYLRIAPELYLKRLVVGG
HHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADFNDLMDLTEEMLSSIATELCGSPQL
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PYGEHTVDFGGPYARLSMLDAIKKYNPDNATIQSMTYEEVKDVEFMRDLAKSLGMTVEKF
CCCCCEECCCCHHHHHHHHHHHHHCCCCCCEEHHCCHHHHHHHHHHHHHHHHHCCHHHHH
WTCGQLLEEIFGETAETQLMQPTFITGYPADISPLARRNDDNHFITDRFEFFIGGREVAN
HHHHHHHHHHHCCCHHHHHCCCEEECCCCCCCCHHHHCCCCCCEEHHHHHHEECCHHHHH
GFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML
HHHHCCCCCCCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCHHHHHHH
FTNTHTIRDVILFPAMRPQAN
HHCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TEQTQDENKLIAERRAKLEHIRTNCPANGHPNNFDRKHKAADIQAEFGHNTKEELEGMG
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCHHHCCCCCHHHHHCCC
IERSIAGRVMAKRGPFLVIQDVSGRIQAYAGKDVQKDLKEKFLGLDIGDIIGVTGQLHLS
CCHHHHHHHHHCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHCCCHHHHHCCCCEEEEC
GKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRYVDLIVNEDSREAFIMRSKVV
CCCCEEECHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SAIRNFMVKKEFMEVETPMMHSIPGGASARPFETHHNALDIAMYLRIAPELYLKRLVVGG
HHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADFNDLMDLTEEMLSSIATELCGSPQL
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PYGEHTVDFGGPYARLSMLDAIKKYNPDNATIQSMTYEEVKDVEFMRDLAKSLGMTVEKF
CCCCCEECCCCHHHHHHHHHHHHHCCCCCCEEHHCCHHHHHHHHHHHHHHHHHCCHHHHH
WTCGQLLEEIFGETAETQLMQPTFITGYPADISPLARRNDDNHFITDRFEFFIGGREVAN
HHHHHHHHHHHCCCHHHHHCCCEEECCCCCCCCHHHHCCCCCCEEHHHHHHEECCHHHHH
GFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML
HHHHCCCCCCCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCHHHHHHH
FTNTHTIRDVILFPAMRPQAN
HHCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA