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The map label for this gene is cysH [H]

Identifier: 157960483

GI number: 157960483

Start: 811629

End: 812384

Strand: Direct

Name: cysH [H]

Synonym: Spea_0655

Alternate gene names: 157960483

Gene position: 811629-812384 (Clockwise)

Preceding gene: 157960482

Following gene: 157960484

Centisome position: 15.68

GC content: 48.02

Gene sequence:

>756_bases
ATGGCTAAAGACTCTGTAATTTCGGCCGATGCGCTCAAGGCTTTATTGAGCGCGCCTAAGGTCGAGCAACAAGCTGAACT
GGCACGGGTGAATGCCTTCTTAGCAGAGCTAAGCCCTGCACAGCGAGTGCGTTGGGCATTAGAGTATCTGCCGGGAAATC
ATGCTCTATCTTCGAGTTTTGGCATTCAGGCTGCGGTCATGCTGCATATGGTGAGCAGCGAAAAAGCAGATATTCCTGTG
CTATTGACCGATACTGGCTACCTGTTTAGCGAGACTTATCAGTTTATCGATGAGTTGACCGAGCGATTGAAGCTTAACTT
GAAGGTGTTCTCATCGCCTTTCAGTTCTGCCTGGCAAGAAGCGCGCTTTGGTAAGTTGTGGGAGCAAGATATTGATGGTT
TGGATAAATATAACCAGATCAATAAAGTGGAGCCGATGCAACGAGCCTTAGCCGAGCAAGAGGTGGGCACTTGGTTTGCA
GGGCTGAGACGTTCACAATCGAGCACCCGTGAGGCTCTGCCTATTTTAGCTATTCATGGCGAGCGCTATAAGATTTTGCC
GATTATCGATTGGAATAATAAACAGGTGCATGAGTATTTAACCGAGTTTGAGTTGCCATATCACCCATTATGGGAGCAAG
GATATGTCTCAGTCGGTGACACGCACTCGAGTAAACCTCTTGAGCTAGGGATGAGTGAAGAGGAGACCCGCTTTAACGGC
TTAAAACGTGAATGTGGTCTGCACTACGACATCTAG

Upstream 100 bases:

>100_bases
GCACTATATGTTGCCGAGCGTCAAGCCGGTGAAACGTTTGGTAACTATACCGTACGCAGTGGTGTGGTTGCCGCTGTACT
CGATGCGGCTAAGGATTTTC

Downstream 100 bases:

>100_bases
TTTTTGCTGGTGTGGTGATTCAATTCTTGGGCTGTTAAGTGGAACAAACGTACCACTTAATAGCCCGAAAGCTGTAATAT
TGTATGCAAAATGCGTGTTT

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MAKDSVISADALKALLSAPKVEQQAELARVNAFLAELSPAQRVRWALEYLPGNHALSSSFGIQAAVMLHMVSSEKADIPV
LLTDTGYLFSETYQFIDELTERLKLNLKVFSSPFSSAWQEARFGKLWEQDIDGLDKYNQINKVEPMQRALAEQEVGTWFA
GLRRSQSSTREALPILAIHGERYKILPIIDWNNKQVHEYLTEFELPYHPLWEQGYVSVGDTHSSKPLELGMSEEETRFNG
LKRECGLHYDI

Sequences:

>Translated_251_residues
MAKDSVISADALKALLSAPKVEQQAELARVNAFLAELSPAQRVRWALEYLPGNHALSSSFGIQAAVMLHMVSSEKADIPV
LLTDTGYLFSETYQFIDELTERLKLNLKVFSSPFSSAWQEARFGKLWEQDIDGLDKYNQINKVEPMQRALAEQEVGTWFA
GLRRSQSSTREALPILAIHGERYKILPIIDWNNKQVHEYLTEFELPYHPLWEQGYVSVGDTHSSKPLELGMSEEETRFNG
LKRECGLHYDI
>Mature_250_residues
AKDSVISADALKALLSAPKVEQQAELARVNAFLAELSPAQRVRWALEYLPGNHALSSSFGIQAAVMLHMVSSEKADIPVL
LTDTGYLFSETYQFIDELTERLKLNLKVFSSPFSSAWQEARFGKLWEQDIDGLDKYNQINKVEPMQRALAEQEVGTWFAG
LRRSQSSTREALPILAIHGERYKILPIIDWNNKQVHEYLTEFELPYHPLWEQGYVSVGDTHSSKPLELGMSEEETRFNGL
KRECGLHYDI

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=237, Percent_Identity=62.4472573839662, Blast_Score=313, Evalue=8e-87,
Organism=Saccharomyces cerevisiae, GI6325425, Length=240, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR011800
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 28546; Mature: 28414

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDSVISADALKALLSAPKVEQQAELARVNAFLAELSPAQRVRWALEYLPGNHALSSSF
CCCCHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCC
GIQAAVMLHMVSSEKADIPVLLTDTGYLFSETYQFIDELTERLKLNLKVFSSPFSSAWQE
CHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHH
ARFGKLWEQDIDGLDKYNQINKVEPMQRALAEQEVGTWFAGLRRSQSSTREALPILAIHG
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
ERYKILPIIDWNNKQVHEYLTEFELPYHPLWEQGYVSVGDTHSSKPLELGMSEEETRFNG
CCEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCEECCCCHHHHHHHH
LKRECGLHYDI
HHHHCCCCCCC
>Mature Secondary Structure 
AKDSVISADALKALLSAPKVEQQAELARVNAFLAELSPAQRVRWALEYLPGNHALSSSF
CCCHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCC
GIQAAVMLHMVSSEKADIPVLLTDTGYLFSETYQFIDELTERLKLNLKVFSSPFSSAWQE
CHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHH
ARFGKLWEQDIDGLDKYNQINKVEPMQRALAEQEVGTWFAGLRRSQSSTREALPILAIHG
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
ERYKILPIIDWNNKQVHEYLTEFELPYHPLWEQGYVSVGDTHSSKPLELGMSEEETRFNG
CCEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCEECCCCHHHHHHHH
LKRECGLHYDI
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]