Click here to switch to the map view.

The map label for this gene is cysI [H]

Identifier: 157960482

GI number: 157960482

Start: 809939

End: 811636

Strand: Direct

Name: cysI [H]

Synonym: Spea_0654

Alternate gene names: 157960482

Gene position: 809939-811636 (Clockwise)

Preceding gene: 157960481

Following gene: 157960483

Centisome position: 15.65

GC content: 49.76

Gene sequence:

>1698_bases
ATGAGTGAGCAGAAGTTAGCAGTAAACGAATATTTGAAAACCGACAGTGATTATCTGCGTGGGACTATCCAAGAGGGCTT
GGATACGGCCGTTACGGGCGCATTTAGCGAAGGCGATCAGCAGTTGATCAAGTTTCACGGCTTCTATCAACAAGATGACC
GCGACCTGCGTAACGAGCGTAAAGAGCAAAAACTAGAGCCCCTATATAGCTTTATGTTGCGTGCCCGTGTTGCAGGTGGC
GTATGCTCACCTGAGCAGTGGTTAGCGGTCGATAAAATTGCATCGAACCTTACGAGCTCGAACAGCATCCGTCTGACTAC
GCGTCAGACATTCCAGTATCACGGTATTCCAAAGCGTAATTTGAAGACCATCATTCAAGATCTTGACCGAGAAGCCTTAG
ACTCGATTGCGGCATGTGGTGACGTTAACCGTAACGTGATGTGTAATCCTAACCCTGTCGAGTCAAAGCTACATCAACAA
GCTTACGCCTATGCCAAGCAGTTGTCTGACAACATGTTGCCGCACACTAAGGCTTATGCCGAGATCTGGTTAGACGATGA
GAAGCTAGTGACCACTGAAGGTGAAGAGGTAGAGCCGGTATACGGTAAGACCTATCTGCCACGTAAATTTAAGATGGCCG
TTGCCGTACCACCGGATAATGATGTGGATGTGTACACCAATGACTTAGGTTTTGTTGCCGTAGCCGAAGGTGATGAGCTC
GTGGGCTTTAACATGGTTGCCGGTGGCGGCATGGGCTCAACCCATGGTGAGGTGTCTACCTTCCCGCGTTTGGCTGATGA
TTTTGGTTACATTAAGGCCGAGGATACGCTTAAGTTTGCCGAAGCTGTGATGACGATTCAGCGCGACTGGGGTAACCGAG
AAAACCGTAAGCTGTCTCGTTTAAAGTACACCATAGTAAAACACGGTTTCGAAAGCTTTAAAGCTGAAATCGAAGCGCGC
ACCGGCATTAAGTTTGAGCCAAAGCGTGATGTAGTGATTGGCGATCGCGGTGACCGTTATGGCTGGAAGCAAGGCGTCGA
TGATAACTGGCACTTAACCCTGTTTATTGAAGGCGGCCGCGTAAAAGATTTTCCGGGTCAGCCATTGCAAACGGGGCTAC
GTGAGATTGCCAAGGTGCATCAAGGCGATTTTCGCATGACTTCCAACCAGAATATTATCATTGCCGGTGTACCGAGCGCA
GACAAAGCGTTGATTGAAGGGCTAGCGAGACAACACGGATTACTCGGCAAGCTGATAACCGAAACCCGTGGTCACTCGAT
TGCCTGTGTGGCGCTGCCAACTTGTGCCTTAGCCATGGCAGAAGCTGAGCGTTACTTCCCGGACTTTTTAACTAAGGTTG
AAGCCTTGCAAGAGAAGCACGGTTTTCTCGACCAGGGCATAGTCATTCGTATGACGGGCTGCCCTAACGGTTGTGCTCGA
CCTTTTGCGGCCGAAATTGGTCTAGTGGGTAAGGCGCCTGGTCGCTATAACCTCTATCTAGGTGCTAGCTTTGAGGGAAC
GCGCTTGAATAAGCTCTATCGCGAGAATATTCAAGAAGCGGAGATCTTGGCTGAACTCGACGCCCTATTTGCACTATATG
TTGCCGAGCGTCAAGCCGGTGAAACGTTTGGTAACTATACCGTACGCAGTGGTGTGGTTGCCGCTGTACTCGATGCGGCT
AAGGATTTTCATGGCTAA

Upstream 100 bases:

>100_bases
CGTTTACTAAGACTCAGCTTTAAACGAGTAAAGCAATGATGAGCGACGCTAGAGTTAACTAGCGCGAGTGCAGAAAAGAA
TTTTAAGAGAGTACCTAGCT

Downstream 100 bases:

>100_bases
AGACTCTGTAATTTCGGCCGATGCGCTCAAGGCTTTATTGAGCGCGCCTAAGGTCGAGCAACAAGCTGAACTGGCACGGG
TGAATGCCTTCTTAGCAGAG

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP [H]

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MSEQKLAVNEYLKTDSDYLRGTIQEGLDTAVTGAFSEGDQQLIKFHGFYQQDDRDLRNERKEQKLEPLYSFMLRARVAGG
VCSPEQWLAVDKIASNLTSSNSIRLTTRQTFQYHGIPKRNLKTIIQDLDREALDSIAACGDVNRNVMCNPNPVESKLHQQ
AYAYAKQLSDNMLPHTKAYAEIWLDDEKLVTTEGEEVEPVYGKTYLPRKFKMAVAVPPDNDVDVYTNDLGFVAVAEGDEL
VGFNMVAGGGMGSTHGEVSTFPRLADDFGYIKAEDTLKFAEAVMTIQRDWGNRENRKLSRLKYTIVKHGFESFKAEIEAR
TGIKFEPKRDVVIGDRGDRYGWKQGVDDNWHLTLFIEGGRVKDFPGQPLQTGLREIAKVHQGDFRMTSNQNIIIAGVPSA
DKALIEGLARQHGLLGKLITETRGHSIACVALPTCALAMAEAERYFPDFLTKVEALQEKHGFLDQGIVIRMTGCPNGCAR
PFAAEIGLVGKAPGRYNLYLGASFEGTRLNKLYRENIQEAEILAELDALFALYVAERQAGETFGNYTVRSGVVAAVLDAA
KDFHG

Sequences:

>Translated_565_residues
MSEQKLAVNEYLKTDSDYLRGTIQEGLDTAVTGAFSEGDQQLIKFHGFYQQDDRDLRNERKEQKLEPLYSFMLRARVAGG
VCSPEQWLAVDKIASNLTSSNSIRLTTRQTFQYHGIPKRNLKTIIQDLDREALDSIAACGDVNRNVMCNPNPVESKLHQQ
AYAYAKQLSDNMLPHTKAYAEIWLDDEKLVTTEGEEVEPVYGKTYLPRKFKMAVAVPPDNDVDVYTNDLGFVAVAEGDEL
VGFNMVAGGGMGSTHGEVSTFPRLADDFGYIKAEDTLKFAEAVMTIQRDWGNRENRKLSRLKYTIVKHGFESFKAEIEAR
TGIKFEPKRDVVIGDRGDRYGWKQGVDDNWHLTLFIEGGRVKDFPGQPLQTGLREIAKVHQGDFRMTSNQNIIIAGVPSA
DKALIEGLARQHGLLGKLITETRGHSIACVALPTCALAMAEAERYFPDFLTKVEALQEKHGFLDQGIVIRMTGCPNGCAR
PFAAEIGLVGKAPGRYNLYLGASFEGTRLNKLYRENIQEAEILAELDALFALYVAERQAGETFGNYTVRSGVVAAVLDAA
KDFHG
>Mature_564_residues
SEQKLAVNEYLKTDSDYLRGTIQEGLDTAVTGAFSEGDQQLIKFHGFYQQDDRDLRNERKEQKLEPLYSFMLRARVAGGV
CSPEQWLAVDKIASNLTSSNSIRLTTRQTFQYHGIPKRNLKTIIQDLDREALDSIAACGDVNRNVMCNPNPVESKLHQQA
YAYAKQLSDNMLPHTKAYAEIWLDDEKLVTTEGEEVEPVYGKTYLPRKFKMAVAVPPDNDVDVYTNDLGFVAVAEGDELV
GFNMVAGGGMGSTHGEVSTFPRLADDFGYIKAEDTLKFAEAVMTIQRDWGNRENRKLSRLKYTIVKHGFESFKAEIEART
GIKFEPKRDVVIGDRGDRYGWKQGVDDNWHLTLFIEGGRVKDFPGQPLQTGLREIAKVHQGDFRMTSNQNIIIAGVPSAD
KALIEGLARQHGLLGKLITETRGHSIACVALPTCALAMAEAERYFPDFLTKVEALQEKHGFLDQGIVIRMTGCPNGCARP
FAAEIGLVGKAPGRYNLYLGASFEGTRLNKLYRENIQEAEILAELDALFALYVAERQAGETFGNYTVRSGVVAAVLDAAK
DFHG

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate [H]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=561, Percent_Identity=55.9714795008913, Blast_Score=672, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322597, Length=593, Percent_Identity=43.0016863406408, Blast_Score=508, Evalue=1e-145,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 62885; Mature: 62754

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQKLAVNEYLKTDSDYLRGTIQEGLDTAVTGAFSEGDQQLIKFHGFYQQDDRDLRNER
CCCCHHHHHHHHHCCHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHH
KEQKLEPLYSFMLRARVAGGVCSPEQWLAVDKIASNLTSSNSIRLTTRQTFQYHGIPKRN
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCHHH
LKTIIQDLDREALDSIAACGDVNRNVMCNPNPVESKLHQQAYAYAKQLSDNMLPHTKAYA
HHHHHHHHCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
EIWLDDEKLVTTEGEEVEPVYGKTYLPRKFKMAVAVPPDNDVDVYTNDLGFVAVAEGDEL
HEEECCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCEEEEECCCCE
VGFNMVAGGGMGSTHGEVSTFPRLADDFGYIKAEDTLKFAEAVMTIQRDWGNRENRKLSR
EEEEEEECCCCCCCCCCHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHCCCCCHHHHHH
LKYTIVKHGFESFKAEIEARTGIKFEPKRDVVIGDRGDRYGWKQGVDDNWHLTLFIEGGR
HHHHHHHHCHHHHHHHHHHCCCCEECCCCCEEECCCCCCCCCCCCCCCCEEEEEEEECCE
VKDFPGQPLQTGLREIAKVHQGDFRMTSNQNIIIAGVPSADKALIEGLARQHGLLGKLIT
ECCCCCCHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHCHHHHHHH
ETRGHSIACVALPTCALAMAEAERYFPDFLTKVEALQEKHGFLDQGIVIRMTGCPNGCAR
HCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
PFAAEIGLVGKAPGRYNLYLGASFEGTRLNKLYRENIQEAEILAELDALFALYVAERQAG
CHHHHCCCEECCCCCEEEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHC
ETFGNYTVRSGVVAAVLDAAKDFHG
CHHCCEEHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEQKLAVNEYLKTDSDYLRGTIQEGLDTAVTGAFSEGDQQLIKFHGFYQQDDRDLRNER
CCCHHHHHHHHHCCHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHH
KEQKLEPLYSFMLRARVAGGVCSPEQWLAVDKIASNLTSSNSIRLTTRQTFQYHGIPKRN
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCHHH
LKTIIQDLDREALDSIAACGDVNRNVMCNPNPVESKLHQQAYAYAKQLSDNMLPHTKAYA
HHHHHHHHCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
EIWLDDEKLVTTEGEEVEPVYGKTYLPRKFKMAVAVPPDNDVDVYTNDLGFVAVAEGDEL
HEEECCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCEEEEECCCCE
VGFNMVAGGGMGSTHGEVSTFPRLADDFGYIKAEDTLKFAEAVMTIQRDWGNRENRKLSR
EEEEEEECCCCCCCCCCHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHCCCCCHHHHHH
LKYTIVKHGFESFKAEIEARTGIKFEPKRDVVIGDRGDRYGWKQGVDDNWHLTLFIEGGR
HHHHHHHHCHHHHHHHHHHCCCCEECCCCCEEECCCCCCCCCCCCCCCCEEEEEEEECCE
VKDFPGQPLQTGLREIAKVHQGDFRMTSNQNIIIAGVPSADKALIEGLARQHGLLGKLIT
ECCCCCCHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHCHHHHHHH
ETRGHSIACVALPTCALAMAEAERYFPDFLTKVEALQEKHGFLDQGIVIRMTGCPNGCAR
HCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
PFAAEIGLVGKAPGRYNLYLGASFEGTRLNKLYRENIQEAEILAELDALFALYVAERQAG
CHHHHCCCEECCCCCEEEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHC
ETFGNYTVRSGVVAAVLDAAKDFHG
CHHCCEEHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA