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The map label for this gene is metJ [H]

Identifier: 157960388

GI number: 157960388

Start: 686241

End: 686555

Strand: Reverse

Name: metJ [H]

Synonym: Spea_0559

Alternate gene names: 157960388

Gene position: 686555-686241 (Counterclockwise)

Preceding gene: 157960395

Following gene: 157960387

Centisome position: 13.27

GC content: 44.13

Gene sequence:

>315_bases
ATGACTGAGTGGAATGGCGATTATATCAGCCCATATGCTGAACACGGCAAAAAGAATGAGCAAGTGAAAAAAATTACTGT
GTCCATTCCTTTGAAAGTACTCAAAGTACTTACCGATGAACGCACACGCCGCCAAGTGAACAACTTGCGTCACGCCACCA
ATAGTGAACTGTTATGCGAGGCATTCTTGCATGCGTACACAGGTCAGCCACTACCAAACGACGCAGATCTTACTAAAGAT
AAACCTGACAGCATGCCTGCTGAGGTTAAACGTCTAATGGATGAAATGGGCATTGAGTGGGAAGAACTTGAATAA

Upstream 100 bases:

>100_bases
GGAAATCTATTTGCAATTGTCATAAATAAGAGGCAATAATTTGACTGCACAATTTAAAGGGTTAAAATCTGCCCCGAATT
TTGAAATAACAGGTGAGTCA

Downstream 100 bases:

>100_bases
TCTTCATCGATTATCGACAAACGTGATCTTCAGATAAGGACTGCTTAGCAGTCCTTATCTGTTTTTGGCCTTGAATGATG
GCTTAGCTCTATTAATCTAT

Product: transcriptional repressor protein MetJ

Products: NA

Alternate protein names: Met regulon regulatory protein metJ [H]

Number of amino acids: Translated: 104; Mature: 103

Protein sequence:

>104_residues
MTEWNGDYISPYAEHGKKNEQVKKITVSIPLKVLKVLTDERTRRQVNNLRHATNSELLCEAFLHAYTGQPLPNDADLTKD
KPDSMPAEVKRLMDEMGIEWEELE

Sequences:

>Translated_104_residues
MTEWNGDYISPYAEHGKKNEQVKKITVSIPLKVLKVLTDERTRRQVNNLRHATNSELLCEAFLHAYTGQPLPNDADLTKD
KPDSMPAEVKRLMDEMGIEWEELE
>Mature_103_residues
TEWNGDYISPYAEHGKKNEQVKKITVSIPLKVLKVLTDERTRRQVNNLRHATNSELLCEAFLHAYTGQPLPNDADLTKDK
PDSMPAEVKRLMDEMGIEWEELE

Specific function: This regulatory protein, when combined with SAM (S- adenosylmethionine) represses the expression of the methionine regulon and of enzymes involved in SAM synthesis [H]

COG id: COG3060

COG function: function code KE; Transcriptional regulator of met regulon

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metJ family [H]

Homologues:

Organism=Escherichia coli, GI1790373, Length=98, Percent_Identity=80.6122448979592, Blast_Score=172, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002084
- InterPro:   IPR010985
- ProDom:   PD020365 [H]

Pfam domain/function: PF01340 MetJ [H]

EC number: NA

Molecular weight: Translated: 12011; Mature: 11880

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEWNGDYISPYAEHGKKNEQVKKITVSIPLKVLKVLTDERTRRQVNNLRHATNSELLCE
CCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHH
AFLHAYTGQPLPNDADLTKDKPDSMPAEVKRLMDEMGIEWEELE
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TEWNGDYISPYAEHGKKNEQVKKITVSIPLKVLKVLTDERTRRQVNNLRHATNSELLCE
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHH
AFLHAYTGQPLPNDADLTKDKPDSMPAEVKRLMDEMGIEWEELE
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]