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The map label for this gene is yafC [H]

Identifier: 157960385

GI number: 157960385

Start: 682998

End: 683912

Strand: Direct

Name: yafC [H]

Synonym: Spea_0556

Alternate gene names: 157960385

Gene position: 682998-683912 (Clockwise)

Preceding gene: 157960382

Following gene: 157960386

Centisome position: 13.2

GC content: 45.14

Gene sequence:

>915_bases
ATGTATCAAGACTTAAATTTAGCAGATGTTCGTGCCTTTACCGTGATTGCGGAAAAGGGCAGTTTTACATTAGCAGCCGA
AAAGCTCGGTTGTTCTCGCTCTCACCTTTCTAAACAATTAACACAGCTTGAGCGCTACCTTGGGGTGACATTGATTACTC
GCACCACGCGAGCCCAGCGGTTAACTGAGCAGGGACAGTTTTTCTATGAACGCTGTCAGCATGCCTTAGATGTGATGGAG
CAAGCGGTTGCAAAGACGGTCGATGATGCTCACAACCTGCAGGGTAAGATCAATATCAACTGTGTGGGCGGCTATATCGG
TGAGGAGATAGTGAATCAGTTGGTGAATGACTTTATGAGTCTGCATCCTAATATTCATATCCACCTCGACTTTAGTAGCC
AGCGAGTCGATTTGGTGCTGGATGAATTTGATCTAGTCTTTCGAATGGGGGAGCTAGAGGATTCAGGCTTGGTGGCAAGA
AAGTTGATGGATATCGCTAATTGCACCTTGGCATCGCCAAGATATATCGCTAAACATGGTCGCCCTGAAAATCCAAAAGA
ATTAAAGAGTCATGCCTGTGTAACTGGCTCGGTTTACCATTGGAATTATCATCGCAGATCGGATAAAACTCAGAAGACCG
AGGTGACCATTACTGGGGCATTGTTGTGTAAAAATGGCAGAGTAATGAAAAGTAGTGCATTAGCCGGAAATGGCATTGTA
CGCCTGCCGGAGATCTATTGTAGTGCAGAGCTAAACAAGGGGGAGTTAGTGCATGTATTCGATGACTGGACTATATCGGA
TACCCCGCTTTATCTGCTGTATAGCAAAGACCAATTTCAGCCCGCTAGGCTCAGGCAGTTTATAGAATTTACCACACAGC
ACTTTATGAAGTATGTCGAATTGCCAAATGCTTAG

Upstream 100 bases:

>100_bases
CCAACAGATTATTGTTGATATATAATCTACAGAGAATATGATTGTTTACATATGACGCCTAATATCGCGCTGTACTCTGA
GATAACACGAGTTTCTATTT

Downstream 100 bases:

>100_bases
GCTGGCTGAGTTAACACGCTTATCTAGGCAATCGTAACTTATCCACCTTTTGGACCAATTGATATGGAAATGTTGATTTA
TGGTTAAGATTCACCTCCTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MYQDLNLADVRAFTVIAEKGSFTLAAEKLGCSRSHLSKQLTQLERYLGVTLITRTTRAQRLTEQGQFFYERCQHALDVME
QAVAKTVDDAHNLQGKININCVGGYIGEEIVNQLVNDFMSLHPNIHIHLDFSSQRVDLVLDEFDLVFRMGELEDSGLVAR
KLMDIANCTLASPRYIAKHGRPENPKELKSHACVTGSVYHWNYHRRSDKTQKTEVTITGALLCKNGRVMKSSALAGNGIV
RLPEIYCSAELNKGELVHVFDDWTISDTPLYLLYSKDQFQPARLRQFIEFTTQHFMKYVELPNA

Sequences:

>Translated_304_residues
MYQDLNLADVRAFTVIAEKGSFTLAAEKLGCSRSHLSKQLTQLERYLGVTLITRTTRAQRLTEQGQFFYERCQHALDVME
QAVAKTVDDAHNLQGKININCVGGYIGEEIVNQLVNDFMSLHPNIHIHLDFSSQRVDLVLDEFDLVFRMGELEDSGLVAR
KLMDIANCTLASPRYIAKHGRPENPKELKSHACVTGSVYHWNYHRRSDKTQKTEVTITGALLCKNGRVMKSSALAGNGIV
RLPEIYCSAELNKGELVHVFDDWTISDTPLYLLYSKDQFQPARLRQFIEFTTQHFMKYVELPNA
>Mature_304_residues
MYQDLNLADVRAFTVIAEKGSFTLAAEKLGCSRSHLSKQLTQLERYLGVTLITRTTRAQRLTEQGQFFYERCQHALDVME
QAVAKTVDDAHNLQGKININCVGGYIGEEIVNQLVNDFMSLHPNIHIHLDFSSQRVDLVLDEFDLVFRMGELEDSGLVAR
KLMDIANCTLASPRYIAKHGRPENPKELKSHACVTGSVYHWNYHRRSDKTQKTEVTITGALLCKNGRVMKSSALAGNGIV
RLPEIYCSAELNKGELVHVFDDWTISDTPLYLLYSKDQFQPARLRQFIEFTTQHFMKYVELPNA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=287, Percent_Identity=30.6620209059233, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=28.0405405405405, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1789639, Length=300, Percent_Identity=28.3333333333333, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87081978, Length=294, Percent_Identity=28.2312925170068, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787128, Length=295, Percent_Identity=26.7796610169492, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789440, Length=288, Percent_Identity=27.7777777777778, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787589, Length=276, Percent_Identity=28.9855072463768, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1789173, Length=285, Percent_Identity=23.859649122807, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790011, Length=299, Percent_Identity=23.7458193979933, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786448, Length=254, Percent_Identity=25.9842519685039, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI157672245, Length=154, Percent_Identity=30.5194805194805, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788706, Length=174, Percent_Identity=27.5862068965517, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787601, Length=295, Percent_Identity=23.728813559322, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34572; Mature: 34572

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQDLNLADVRAFTVIAEKGSFTLAAEKLGCSRSHLSKQLTQLERYLGVTLITRTTRAQR
CCCCCCHHHHEEEEEEEECCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCCEEEECHHHHH
LTEQGQFFYERCQHALDVMEQAVAKTVDDAHNLQGKININCVGGYIGEEIVNQLVNDFMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHH
LHPNIHIHLDFSSQRVDLVLDEFDLVFRMGELEDSGLVARKLMDIANCTLASPRYIAKHG
HCCCEEEEEECCCCCEEEEHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHCC
RPENPKELKSHACVTGSVYHWNYHRRSDKTQKTEVTITGALLCKNGRVMKSSALAGNGIV
CCCCHHHHHHCCEEECEEEEEEECCCCCCCCCEEEEEEEEEEECCCCEEECCCCCCCCCE
RLPEIYCSAELNKGELVHVFDDWTISDTPLYLLYSKDQFQPARLRQFIEFTTQHFMKYVE
ECCCEEEECCCCCCCEEEEEECCEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHC
LPNA
CCCC
>Mature Secondary Structure
MYQDLNLADVRAFTVIAEKGSFTLAAEKLGCSRSHLSKQLTQLERYLGVTLITRTTRAQR
CCCCCCHHHHEEEEEEEECCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCCEEEECHHHHH
LTEQGQFFYERCQHALDVMEQAVAKTVDDAHNLQGKININCVGGYIGEEIVNQLVNDFMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHH
LHPNIHIHLDFSSQRVDLVLDEFDLVFRMGELEDSGLVARKLMDIANCTLASPRYIAKHG
HCCCEEEEEECCCCCEEEEHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHCC
RPENPKELKSHACVTGSVYHWNYHRRSDKTQKTEVTITGALLCKNGRVMKSSALAGNGIV
CCCCHHHHHHCCEEECEEEEEEECCCCCCCCCEEEEEEEEEEECCCCEEECCCCCCCCCE
RLPEIYCSAELNKGELVHVFDDWTISDTPLYLLYSKDQFQPARLRQFIEFTTQHFMKYVE
ECCCEEEECCCCCCCEEEEEECCEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHC
LPNA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]