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The map label for this gene is fdoH [H]

Identifier: 157960268

GI number: 157960268

Start: 546608

End: 547510

Strand: Direct

Name: fdoH [H]

Synonym: Spea_0439

Alternate gene names: 157960268

Gene position: 546608-547510 (Clockwise)

Preceding gene: 157960267

Following gene: 157960269

Centisome position: 10.56

GC content: 49.5

Gene sequence:

>903_bases
ATGTCTGCTCAAGATATCATTTTAAGCTCTGGCACATCAGAGCTCACTCCTGCAGCGCAGGTACGCCGTAAGTTAAGTAA
GGTGGCCAAGTTATTCGACGCCACTAAGTGTAACGGCTGTAAGGCTTGCCAGGTGTCTTGTTCTGAATGGAACGATCTGC
GTGCGCCAGTGGGGAGCTTCCAAGGTTCTTACCAGAACCCAATGGACCTGTCTTCAGAGTGTTGGACACTGATGAAGTAT
CAGGAAGTGGAAGAGAACAATAAACTAAATTGGCGTTTCGCCCACAGCGCATGTATGCACTGTGACGATCCTGCCTGTTT
AACCGCTTGTTCGACCAAAGGCGCCATCGTGCAACGCGCAAACGGCGTGGTGGATTTTGATTCTGATAAGTGTACTGGTT
GTGGCTACTGCGTTTCGGCTTGTCCGTTCGATGTACCAAGACTCGACCCTATCGATCAAAAGGCTTACAAGTGCACCATG
TGTTCAGACCGTATCCAAGTGGGCCAAGAGCCTGCCTGTGTGAAGTCTTGTATTACTGGCGCACTGAAGTACGGCACCCG
TGAAGACATGCTGTTTATTGCAGACTTACGGATTAAAGAGCTGCGTAAGCGCGGCTATGTTAATGCCGGTCTATACAACC
CAGAAGGTGTGGGCGGTACAGGCATGATTATGATTTTGCATGACATTAATCAGCCACAAAACTACGGCCTACCAGCCGAT
CCGAAAACGAGTGTGCCAATTAAGTTATGGCAAGACGTGGTTAAGCCATTGGGTGCTGCAGGTATCATCGCAACAGTTGC
TGTTGCGTGTCTACACCGCATCACTGTGGGGCGTAACCTTGTTGAAGAGGACGAACCTGCACAGTTCGACCACACTCACT
CAGATAAGTCAGATAAGGAGTAA

Upstream 100 bases:

>100_bases
GTTGTAACGTACTGACCACAGAAGTGGGCGATGCAAATACACAGGTGCCTGAATTTAAAGCATTCCTTGTGGATATTGCT
AAGGCTGAGGGAGTTTAATC

Downstream 100 bases:

>100_bases
CGCTCATGCATAAGCAGAATATGATTTTGCGCCATAAGTTGTTCGACCGCATCTGTCACTGGTTTGTGGTTGCAACGGGT
TTGGTGACATTCCTAACCGG

Product: formate dehydrogenase subunit beta

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSAQDIILSSGTSELTPAAQVRRKLSKVAKLFDATKCNGCKACQVSCSEWNDLRAPVGSFQGSYQNPMDLSSECWTLMKY
QEVEENNKLNWRFAHSACMHCDDPACLTACSTKGAIVQRANGVVDFDSDKCTGCGYCVSACPFDVPRLDPIDQKAYKCTM
CSDRIQVGQEPACVKSCITGALKYGTREDMLFIADLRIKELRKRGYVNAGLYNPEGVGGTGMIMILHDINQPQNYGLPAD
PKTSVPIKLWQDVVKPLGAAGIIATVAVACLHRITVGRNLVEEDEPAQFDHTHSDKSDKE

Sequences:

>Translated_300_residues
MSAQDIILSSGTSELTPAAQVRRKLSKVAKLFDATKCNGCKACQVSCSEWNDLRAPVGSFQGSYQNPMDLSSECWTLMKY
QEVEENNKLNWRFAHSACMHCDDPACLTACSTKGAIVQRANGVVDFDSDKCTGCGYCVSACPFDVPRLDPIDQKAYKCTM
CSDRIQVGQEPACVKSCITGALKYGTREDMLFIADLRIKELRKRGYVNAGLYNPEGVGGTGMIMILHDINQPQNYGLPAD
PKTSVPIKLWQDVVKPLGAAGIIATVAVACLHRITVGRNLVEEDEPAQFDHTHSDKSDKE
>Mature_299_residues
SAQDIILSSGTSELTPAAQVRRKLSKVAKLFDATKCNGCKACQVSCSEWNDLRAPVGSFQGSYQNPMDLSSECWTLMKYQ
EVEENNKLNWRFAHSACMHCDDPACLTACSTKGAIVQRANGVVDFDSDKCTGCGYCVSACPFDVPRLDPIDQKAYKCTMC
SDRIQVGQEPACVKSCITGALKYGTREDMLFIADLRIKELRKRGYVNAGLYNPEGVGGTGMIMILHDINQPQNYGLPADP
KTSVPIKLWQDVVKPLGAAGIIATVAVACLHRITVGRNLVEEDEPAQFDHTHSDKSDKE

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=298, Percent_Identity=52.6845637583893, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI1787749, Length=287, Percent_Identity=54.006968641115, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI1789370, Length=186, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI2367345, Length=181, Percent_Identity=30.939226519337, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787872, Length=168, Percent_Identity=33.9285714285714, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787122, Length=182, Percent_Identity=33.5164835164835, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI226510944, Length=185, Percent_Identity=30.8108108108108, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787963, Length=155, Percent_Identity=24.5161290322581, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787478, Length=88, Percent_Identity=37.5, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787740, Length=105, Percent_Identity=38.0952380952381, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082180, Length=169, Percent_Identity=27.2189349112426, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 32841; Mature: 32710

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
6.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQDIILSSGTSELTPAAQVRRKLSKVAKLFDATKCNGCKACQVSCSEWNDLRAPVGSF
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHCCHHHC
QGSYQNPMDLSSECWTLMKYQEVEENNKLNWRFAHSACMHCDDPACLTACSTKGAIVQRA
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCHHHHHHCCCCCHHHHHC
NGVVDFDSDKCTGCGYCVSACPFDVPRLDPIDQKAYKCTMCSDRIQVGQEPACVKSCITG
CCCEECCCCCCCCCCHHHHCCCCCCCCCCCCCCHHEEEHHHHHHHHCCCCHHHHHHHHHH
ALKYGTREDMLFIADLRIKELRKRGYVNAGLYNPEGVGGTGMIMILHDINQPQNYGLPAD
HHHCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCC
PKTSVPIKLWQDVVKPLGAAGIIATVAVACLHRITVGRNLVEEDEPAQFDHTHSDKSDKE
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAQDIILSSGTSELTPAAQVRRKLSKVAKLFDATKCNGCKACQVSCSEWNDLRAPVGSF
CCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHCCHHHC
QGSYQNPMDLSSECWTLMKYQEVEENNKLNWRFAHSACMHCDDPACLTACSTKGAIVQRA
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCHHHHHHCCCCCHHHHHC
NGVVDFDSDKCTGCGYCVSACPFDVPRLDPIDQKAYKCTMCSDRIQVGQEPACVKSCITG
CCCEECCCCCCCCCCHHHHCCCCCCCCCCCCCCHHEEEHHHHHHHHCCCCHHHHHHHHHH
ALKYGTREDMLFIADLRIKELRKRGYVNAGLYNPEGVGGTGMIMILHDINQPQNYGLPAD
HHHCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCC
PKTSVPIKLWQDVVKPLGAAGIIATVAVACLHRITVGRNLVEEDEPAQFDHTHSDKSDKE
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]