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The map label for this gene is fdnI [H]

Identifier: 157960269

GI number: 157960269

Start: 547516

End: 548160

Strand: Direct

Name: fdnI [H]

Synonym: Spea_0440

Alternate gene names: 157960269

Gene position: 547516-548160 (Clockwise)

Preceding gene: 157960268

Following gene: 157960270

Centisome position: 10.58

GC content: 47.6

Gene sequence:

>645_bases
ATGCATAAGCAGAATATGATTTTGCGCCATAAGTTGTTCGACCGCATCTGTCACTGGTTTGTGGTTGCAACGGGTTTGGT
GACATTCCTAACCGGCTTTGCTTTCTTCTTCCCATCATTTCAATGGTTGGGGTCGATTGCAGGTACGCCGCAAATGGCGC
GCTTTGTTCACCCTATTGCCGGCTTGATGATGTGTCTGCCGTTAATGGTGATGTTGGTGCGTTATTACCACCATAACAAG
TGGGAAAAGAATGACCTGACATGGATGTTAGCTATCAAGGACGTGATGTTCGAGAACGAGGACAAAATCCCCGCCATCGG
CCATTACAACCCGGGTCAAAAAGTGCTGTTCCGCACCTTCGTGGTGACCTCAATCGCATTGACGATTACTGGCATCATGA
TGTGGCAGCCTTACTTTGCCCCTTATTTTGAAGCAAGCGCCATTCAGTGGGCCATTTTGATCCACGCAATCTGTGCTGTG
ATCATGTTGATCTTCGTGATTGTGCACTTTTGGATGGCAACGTGGGTAGAAGGATCGGTTGCTGGCATGTTGTACGGCTA
TGTGTCGCGGGCTTGGTGCAAGAAGCATCACCCTAACATGCTAAACGATCCTGAATTAAAGACTAATAAGCAGGAAAAAC
ACTAA

Upstream 100 bases:

>100_bases
GTCTACACCGCATCACTGTGGGGCGTAACCTTGTTGAAGAGGACGAACCTGCACAGTTCGACCACACTCACTCAGATAAG
TCAGATAAGGAGTAACGCTC

Downstream 100 bases:

>100_bases
TTATGAGCACAGCAATACTCGACGCTGAAAAGATCCCCGGCCAGTCACTGGATATTAAAACCGTGATCGCAGCTGACCCA
AAAGCCGTTTATCAATTTAG

Product: formate dehydrogenase subunit gamma

Products: CO2; NADH

Alternate protein names: Anaerobic formate dehydrogenase cytochrome b556 subunit; Formate dehydrogenase-N subunit gamma; FDH-N subunit gamma [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MHKQNMILRHKLFDRICHWFVVATGLVTFLTGFAFFFPSFQWLGSIAGTPQMARFVHPIAGLMMCLPLMVMLVRYYHHNK
WEKNDLTWMLAIKDVMFENEDKIPAIGHYNPGQKVLFRTFVVTSIALTITGIMMWQPYFAPYFEASAIQWAILIHAICAV
IMLIFVIVHFWMATWVEGSVAGMLYGYVSRAWCKKHHPNMLNDPELKTNKQEKH

Sequences:

>Translated_214_residues
MHKQNMILRHKLFDRICHWFVVATGLVTFLTGFAFFFPSFQWLGSIAGTPQMARFVHPIAGLMMCLPLMVMLVRYYHHNK
WEKNDLTWMLAIKDVMFENEDKIPAIGHYNPGQKVLFRTFVVTSIALTITGIMMWQPYFAPYFEASAIQWAILIHAICAV
IMLIFVIVHFWMATWVEGSVAGMLYGYVSRAWCKKHHPNMLNDPELKTNKQEKH
>Mature_214_residues
MHKQNMILRHKLFDRICHWFVVATGLVTFLTGFAFFFPSFQWLGSIAGTPQMARFVHPIAGLMMCLPLMVMLVRYYHHNK
WEKNDLTWMLAIKDVMFENEDKIPAIGHYNPGQKVLFRTFVVTSIALTITGIMMWQPYFAPYFEASAIQWAILIHAICAV
IMLIFVIVHFWMATWVEGSVAGMLYGYVSRAWCKKHHPNMLNDPELKTNKQEKH

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. Subunit gamma is the cytochrome b556(FDN) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787750, Length=214, Percent_Identity=42.9906542056075, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1790325, Length=213, Percent_Identity=35.2112676056338, Blast_Score=171, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 24970; Mature: 24970

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
7.0 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
7.0 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKQNMILRHKLFDRICHWFVVATGLVTFLTGFAFFFPSFQWLGSIAGTPQMARFVHPIA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GLMMCLPLMVMLVRYYHHNKWEKNDLTWMLAIKDVMFENEDKIPAIGHYNPGQKVLFRTF
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
VVTSIALTITGIMMWQPYFAPYFEASAIQWAILIHAICAVIMLIFVIVHFWMATWVEGSV
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGMLYGYVSRAWCKKHHPNMLNDPELKTNKQEKH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHKQNMILRHKLFDRICHWFVVATGLVTFLTGFAFFFPSFQWLGSIAGTPQMARFVHPIA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GLMMCLPLMVMLVRYYHHNKWEKNDLTWMLAIKDVMFENEDKIPAIGHYNPGQKVLFRTF
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
VVTSIALTITGIMMWQPYFAPYFEASAIQWAILIHAICAVIMLIFVIVHFWMATWVEGSV
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGMLYGYVSRAWCKKHHPNMLNDPELKTNKQEKH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]