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The map label for this gene is acnB [H]

Identifier: 157960254

GI number: 157960254

Start: 526341

End: 528938

Strand: Direct

Name: acnB [H]

Synonym: Spea_0425

Alternate gene names: 157960254

Gene position: 526341-528938 (Clockwise)

Preceding gene: 157960252

Following gene: 157960256

Centisome position: 10.17

GC content: 47.38

Gene sequence:

>2598_bases
GTGCTAGAAGCATATCGTAAACACGTCGCAGAACGTGCTTCAGAGGGCGTAGTCCCTAAGCCATTAGATGCCCAACAAGT
GGCACAGCTAGTCGAGTTGGTAAAGAATCCACCTGCAGGTGAAGAAGCGTTTATTCTTGATCTGCTTGAAAACCGTATCC
CACCAGGTGTTGATGAAGCCGCGTACGTTAAAGCAGGCTTTTTAGATGCAGTAGCCAAAGGCGAAGTATCATCTTCTATC
TTATCGTCAGAGCGCGCTATCGAGCTGCTTGGCACTATGCAGGGTGGCTACAACATTGAGCCATTGATTGCTCAACTAGA
TAACGACGCACAAGCGCCACTTGCAGTAAAAGCATTGTCTCATACACTGCTTATGTTCGATGCGTATCATGACGTTGTTG
AAAAAATGCAAGCGGGTAACGAGTTCGCTAAACAAGTAGTTCACTCTTGGGCTGATGCCGAGTGGTTCCTATCTCGTCCA
AAACTTGCAGAGAAAATCTCGCTAACCGTATTTAAAGTATCGGGTGAGACTAACACTGATGACCTGTCTCCAGCACCAGA
TGCATGGTCACGTCCAGATATTCCATTGCACGCACTTGCCATGCTTAAGAATGCCCGTGAAGGTATTGTTCCTGATGAAG
CTGGCGAAGTCGGTCCAATCAAAGAGATTGAGTCGCTAAAAACCAAAGGTTTCCCACTGGTTTACGTCGGTGACGTTGTC
GGTACCGGTTCTTCACGTAAGTCTGCAACTAACTCTGTTTTATGGTTCATGGGCGATGATATCCCGAATGTACCAAACAA
GCGTGCGGGTGGTTTCTGTTTGGGTGGCAAAATCGCACCCATCTTCTTTAATACTATGGAAGATGCGGGCGCACTACCAA
TTGAACTAGATGTTAGCAAGATGGAGATGGGCGATGTTATCGACATCTATCCATATCAAGGCATCGTTAAGCGTCATGAT
ACTGATGAAGTTATTTCACAGTTCTCATTGAAGACTGCAGTGCTAATGGATGAGGTGCGCGCTGGTGGTCGTATTCCACT
TATCATCGGTCGTGGTCTAACAGATCGCGCGCGTGAAACGCTAAACCTTGAAGCATCAACTGAGTTTGTTCGCCCACAAG
ATGTGGCTGACACGGGTAAGGGTTATACCCTTGCACAAAAGATGGTCGGTAAGGCATGTGGCGTGACGGGTATTCGTCCA
GGCCAATATTGTGAACCTAAGATGACCTCTGTGGGTTCACAAGATACTACAGGCCCAATGACTCGTGATGAGCTAAAAGA
TTTAGCGTGTCTTGGTTTCAGTGCAGACTTAACCATGCAGTCTTTCTGTCACACGGCGGCTTATCCAAAGCCTGTTGACG
TAAACACTCACCATACGCTGCCTGATTTCATCATGAACCGTGGCGGTGTTGCTCTGCGTCCTGGTGATGGTGTTATTCAC
TCTTGGTTAAACCGTATGTTACTACCTGATACCGTCGGTACCGGTGGTGATTCACATACGCGTTTCCCAATTGGTATTTC
ATTCCCCGCAGGTTCTGGTCTTGTGGCATTCGCTGCTGCAACGGGCGTGATGCCACTGGATATGCCTGAATCAATTTTGG
TGCGCTTTAAAGGTGAAATGCAACCGGGTATCACGTTACGTGACTTGGTACATGCAATCCCACTTAAAGCGATAGAGATG
GGTCTGTTAACGGTTGAGAAAAAAGGCAAGATCAATGCTTTCTCTGGCCGTGTACTAGAAATTGAAGGTCTGGAGCAGCT
TAAAGTTGAGCAGGCATTCGAGCTTTCAGATGCATCTGCTGAGCGCAGTGCGGCAGGTTGTACGATTAAGCTAGACAAAG
AGCCAATCATCGAATACCTAAACTCAAACATCGTCATGCTTAAGTGGATGATTAGCGAGGGTTATGGTGATCGTCGTACT
ATCGAGCGCCGTATCACAGCAATGCAAGAGTGGTTAGCGAATCCTGAGCTTATGGAAGCGGATGGCGATGCAGAATACGC
AGAAGTTATCGAGATCGATCTAGCTGATATCAAAGAACCCATTCTTTGTGCGCCGAACGATCCTGATGATGCGATTCTAC
TTTCATCTGTGGTTAATACTAAGATTGACGAAGTTTTCGTTGGTTCTTGTATGACTAACATTGGTCACTTCCGTGCAACG
GGTAAGATGCTAGATAAATTTGCATCGACATTACCGACACGCTTGTGGATTGCGCCACCGACTAAGATGGATCGCGATCA
ACTAACAGAAGAAGGCTACTACGCTATCTTCGGCCGTGTGGGTGCACGTATCGAGATCCCAGGTTGTTCACTGTGTATGG
GTAACCAGGCACGTGTTGCAGAAGGTGCAACGGTAGTATCGACTTCTACTCGTAACTTCCCGAACCGTCTAGGTACAGGT
GCTAACGTATACTTGGCATCGGCAGAGCTTGCGGCGGTTGCAGCACTTCTAGGTCGTTTACCATCGCCTGATGAGTATCA
GACATACGCTAAAGAGTTAGATGCAACTGCAGCTGACACTTATCGCTACCTAAACTTCGATCAGATTGCCTCGTATACGG
CAAAAGCCGACGAAGTGATCTTCCAATCGGCGGTTTAA

Upstream 100 bases:

>100_bases
CGAGCCTGCCGAGCTGTCAGTTTCAATTTGAAGACGAGCAGTTTCGCTGTTATATCAATCTTAGATTGGTATTGAACGCC
AAACAAAGAGGAATATTGCC

Downstream 100 bases:

>100_bases
GCCAGATTGATATACTGAAGTTGATGCTCTGAAATTGGAGCATTAAAAAGGCCAGCTAAATTAGCTGGCCTTTTTGTTAA
TTCATTTTTAAGCTCCGCTA

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase; 2-methylisocitrate dehydratase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 865; Mature: 865

Protein sequence:

>865_residues
MLEAYRKHVAERASEGVVPKPLDAQQVAQLVELVKNPPAGEEAFILDLLENRIPPGVDEAAYVKAGFLDAVAKGEVSSSI
LSSERAIELLGTMQGGYNIEPLIAQLDNDAQAPLAVKALSHTLLMFDAYHDVVEKMQAGNEFAKQVVHSWADAEWFLSRP
KLAEKISLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIVPDEAGEVGPIKEIESLKTKGFPLVYVGDVV
GTGSSRKSATNSVLWFMGDDIPNVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSKMEMGDVIDIYPYQGIVKRHD
TDEVISQFSLKTAVLMDEVRAGGRIPLIIGRGLTDRARETLNLEASTEFVRPQDVADTGKGYTLAQKMVGKACGVTGIRP
GQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVALRPGDGVIH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPGITLRDLVHAIPLKAIEM
GLLTVEKKGKINAFSGRVLEIEGLEQLKVEQAFELSDASAERSAAGCTIKLDKEPIIEYLNSNIVMLKWMISEGYGDRRT
IERRITAMQEWLANPELMEADGDAEYAEVIEIDLADIKEPILCAPNDPDDAILLSSVVNTKIDEVFVGSCMTNIGHFRAT
GKMLDKFASTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGNQARVAEGATVVSTSTRNFPNRLGTG
ANVYLASAELAAVAALLGRLPSPDEYQTYAKELDATAADTYRYLNFDQIASYTAKADEVIFQSAV

Sequences:

>Translated_865_residues
MLEAYRKHVAERASEGVVPKPLDAQQVAQLVELVKNPPAGEEAFILDLLENRIPPGVDEAAYVKAGFLDAVAKGEVSSSI
LSSERAIELLGTMQGGYNIEPLIAQLDNDAQAPLAVKALSHTLLMFDAYHDVVEKMQAGNEFAKQVVHSWADAEWFLSRP
KLAEKISLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIVPDEAGEVGPIKEIESLKTKGFPLVYVGDVV
GTGSSRKSATNSVLWFMGDDIPNVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSKMEMGDVIDIYPYQGIVKRHD
TDEVISQFSLKTAVLMDEVRAGGRIPLIIGRGLTDRARETLNLEASTEFVRPQDVADTGKGYTLAQKMVGKACGVTGIRP
GQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVALRPGDGVIH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPGITLRDLVHAIPLKAIEM
GLLTVEKKGKINAFSGRVLEIEGLEQLKVEQAFELSDASAERSAAGCTIKLDKEPIIEYLNSNIVMLKWMISEGYGDRRT
IERRITAMQEWLANPELMEADGDAEYAEVIEIDLADIKEPILCAPNDPDDAILLSSVVNTKIDEVFVGSCMTNIGHFRAT
GKMLDKFASTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGNQARVAEGATVVSTSTRNFPNRLGTG
ANVYLASAELAAVAALLGRLPSPDEYQTYAKELDATAADTYRYLNFDQIASYTAKADEVIFQSAV
>Mature_865_residues
MLEAYRKHVAERASEGVVPKPLDAQQVAQLVELVKNPPAGEEAFILDLLENRIPPGVDEAAYVKAGFLDAVAKGEVSSSI
LSSERAIELLGTMQGGYNIEPLIAQLDNDAQAPLAVKALSHTLLMFDAYHDVVEKMQAGNEFAKQVVHSWADAEWFLSRP
KLAEKISLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIVPDEAGEVGPIKEIESLKTKGFPLVYVGDVV
GTGSSRKSATNSVLWFMGDDIPNVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSKMEMGDVIDIYPYQGIVKRHD
TDEVISQFSLKTAVLMDEVRAGGRIPLIIGRGLTDRARETLNLEASTEFVRPQDVADTGKGYTLAQKMVGKACGVTGIRP
GQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVALRPGDGVIH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPGITLRDLVHAIPLKAIEM
GLLTVEKKGKINAFSGRVLEIEGLEQLKVEQAFELSDASAERSAAGCTIKLDKEPIIEYLNSNIVMLKWMISEGYGDRRT
IERRITAMQEWLANPELMEADGDAEYAEVIEIDLADIKEPILCAPNDPDDAILLSSVVNTKIDEVFVGSCMTNIGHFRAT
GKMLDKFASTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGNQARVAEGATVVSTSTRNFPNRLGTG
ANVYLASAELAAVAALLGRLPSPDEYQTYAKELDATAADTYRYLNFDQIASYTAKADEVIFQSAV

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=865, Percent_Identity=81.271676300578, Blast_Score=1444, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=376, Percent_Identity=26.063829787234, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323335, Length=381, Percent_Identity=25.7217847769029, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321429, Length=359, Percent_Identity=23.9554317548747, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 93779; Mature: 93779

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEAYRKHVAERASEGVVPKPLDAQQVAQLVELVKNPPAGEEAFILDLLENRIPPGVDEA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCH
AYVKAGFLDAVAKGEVSSSILSSERAIELLGTMQGGYNIEPLIAQLDNDAQAPLAVKALS
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCHHHHHHH
HTLLMFDAYHDVVEKMQAGNEFAKQVVHSWADAEWFLSRPKLAEKISLTVFKVSGETNTD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHCCCCHHHEEEEEEEEECCCCCCC
DLSPAPDAWSRPDIPLHALAMLKNAREGIVPDEAGEVGPIKEIESLKTKGFPLVYVGDVV
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCEE
GTGSSRKSATNSVLWFMGDDIPNVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEEEEECHHCCCCCEEEEEHHH
MEMGDVIDIYPYQGIVKRHDTDEVISQFSLKTAVLMDEVRAGGRIPLIIGRGLTDRARET
HCCCCEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
LNLEASTEFVRPQDVADTGKGYTLAQKMVGKACGVTGIRPGQYCEPKMTSVGSQDTTGPM
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCC
TRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVALRPGDGVIH
CHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCEEECCCCCHHH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEM
HHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHEECCCC
QPGITLRDLVHAIPLKAIEMGLLTVEKKGKINAFSGRVLEIEGLEQLKVEQAFELSDASA
CCCCCHHHHHHHHCHHHHHHHHEEEECCCCEEECCCCEEEECCCHHHHHHHHHHCCCCCC
ERSAAGCTIKLDKEPIIEYLNSNIVMLKWMISEGYGDRRTIERRITAMQEWLANPELMEA
HHCCCCCEEEECHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEC
DGDAEYAEVIEIDLADIKEPILCAPNDPDDAILLSSVVNTKIDEVFVGSCMTNIGHFRAT
CCCCCCHHHEEEEHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKMLDKFASTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGNQARVA
HHHHHHHHHHCCCEEEECCCCCCCHHHHCCCCCEEEEECCCCEEECCCCCEECCCCCCCC
EGATVVSTSTRNFPNRLGTGANVYLASAELAAVAALLGRLPSPDEYQTYAKELDATAADT
CCCEEEECCCCCCHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHH
YRYLNFDQIASYTAKADEVIFQSAV
HHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLEAYRKHVAERASEGVVPKPLDAQQVAQLVELVKNPPAGEEAFILDLLENRIPPGVDEA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCH
AYVKAGFLDAVAKGEVSSSILSSERAIELLGTMQGGYNIEPLIAQLDNDAQAPLAVKALS
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCHHHHHHH
HTLLMFDAYHDVVEKMQAGNEFAKQVVHSWADAEWFLSRPKLAEKISLTVFKVSGETNTD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHCCCCHHHEEEEEEEEECCCCCCC
DLSPAPDAWSRPDIPLHALAMLKNAREGIVPDEAGEVGPIKEIESLKTKGFPLVYVGDVV
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCEE
GTGSSRKSATNSVLWFMGDDIPNVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEEEEECHHCCCCCEEEEEHHH
MEMGDVIDIYPYQGIVKRHDTDEVISQFSLKTAVLMDEVRAGGRIPLIIGRGLTDRARET
HCCCCEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
LNLEASTEFVRPQDVADTGKGYTLAQKMVGKACGVTGIRPGQYCEPKMTSVGSQDTTGPM
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCC
TRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVALRPGDGVIH
CHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCEEECCCCCHHH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEM
HHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHEECCCC
QPGITLRDLVHAIPLKAIEMGLLTVEKKGKINAFSGRVLEIEGLEQLKVEQAFELSDASA
CCCCCHHHHHHHHCHHHHHHHHEEEECCCCEEECCCCEEEECCCHHHHHHHHHHCCCCCC
ERSAAGCTIKLDKEPIIEYLNSNIVMLKWMISEGYGDRRTIERRITAMQEWLANPELMEA
HHCCCCCEEEECHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEC
DGDAEYAEVIEIDLADIKEPILCAPNDPDDAILLSSVVNTKIDEVFVGSCMTNIGHFRAT
CCCCCCHHHEEEEHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKMLDKFASTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGNQARVA
HHHHHHHHHHCCCEEEECCCCCCCHHHHCCCCCEEEEECCCCEEECCCCCEECCCCCCCC
EGATVVSTSTRNFPNRLGTGANVYLASAELAAVAALLGRLPSPDEYQTYAKELDATAADT
CCCEEEECCCCCCHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHH
YRYLNFDQIASYTAKADEVIFQSAV
HHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 8932712; 9298646; 10585860; 8000525; 11992126 [H]