Click here to switch to the map view.

The map label for this gene is tapB [H]

Identifier: 157960242

GI number: 157960242

Start: 505266

End: 506972

Strand: Reverse

Name: tapB [H]

Synonym: Spea_0413

Alternate gene names: 157960242

Gene position: 506972-505266 (Counterclockwise)

Preceding gene: 157960243

Following gene: 157960241

Centisome position: 9.8

GC content: 47.22

Gene sequence:

>1707_bases
ATGCCAACAACCGGCCTTCACTTAGGGTTATCGACCCTCTTTATTCGCAAAGGTTTGCTTAATGAAAAGCAGCTAAGTGA
AGCCGTGTCTAAAAGTCGCAAGCTCCGTCAGGGGCTTGTGGCTACTTTAGTAAAGGATAACTTGATTAGCGCTCGTGAAA
TCGCAGAGTTATGCTACGAAGAATACGGCACACCGCTATTAGACTTAAACGAGTTCGAGATAGGCTCCATCCCCGAAGAA
TTCCTCAATAAGAAACTGATAGAGAAGCACAAATGCTTGCCACTTTTTAAGCGCGGTAATCGTCTCTATATCGCCACATC
CGACCCTACAAACATAGCAGCTTTAGAAGACTTTCAGTTTAGTGCCGGTTTGCATGCAGAAGCGATTCTAGTCGAGGACG
ATAAGCTCAGTAAAGCTATAGACTCTATTCTCGAAGACGATATTAGCGGCCTAGACTTAAGTGCGATAGACGAAGAAGCC
TTGGCAGGTATAGAAGTTACAGATACAGACAAACGTGATGAGGAAGAAAACAGTGGTAAAGACGACGCGCCGATCGTTAT
CTATATCAATAAAATCCTCACCGATGCCATTCGCAAAGGCGCATCAGATCTGCATTTTGAGCCCTATGAAAAGCGTTACC
GCATCCGTTTTCGCATCGACGGTATTTTACATGAGGTCTCCGAGCCACCAGTAAATCTTTCCGGTCGCATCTCTGCCCGC
TTAAAGGTGATGTCAAAACTTGATATTGCCGAACGCCGAGTGCCGCAAGATGGTCGTATCAAGATGAAGCTATCGCGCAC
CAAATCTATCGACTTTCGTGTCAGCACCTTGCCTACCATCTGGGGCGAGAAGATTGTAATGCGTATCTTGGACTCATCAT
CCGCTCAGCTTGGCATCGAAAAACTCGGCTATGAAGATGACCAAAGATTACTGTATGAAGAGATGCTGCAAAAACCGCAA
GGGATGATCTTGGTAACAGGCCCTACAGGCTCGGGTAAAACGGTATCGCTTTATACTGGCCTTAATATTCTTAACACCGA
AGAGCGTAATATCTCTACCGCCGAAGATCCGGTTGAGATTAATCTCGAAGGGGTCAACCAGGTTCATATCAATCTTAAGG
CAGGTCTAACCTTCGCATCAGCGCTACGTTCATTCCTGCGACAAGACCCCGATGTGGTCATGGTCGGTGAAATTCGAGAT
TTGGAAACTGCTGAAATTGCCATTAAAGCGGCGCAAACCGGGCACTTGGTTCTTTCGACTTTGCATACTAACTCCGCTGC
AGAAACACTCACCCGCTTAATCAACATGGGCGTCCCCGGTTACAACATCGCTAGCTCGGTCAACCTGATTATCGCTCAGC
GGCTATCGAGGCGCCTCTGCCCAACCTGTAAAGCGCCTGAAGCGATCCCAGCAGAAGAGTTATTAAAGCTCGGCTTTAAC
CAGCAACAAGTCGATGCAGGCATTATCCCCCACAAACCCGTGGGTTGTGACCTATGCTCCGGCGGTTATAAAGGCCGAGT
CGGTATCTATGAGGTAATGAAGATGTCGGATGAAATTGCTCGCACTATTATGGAAGGCGGTAATTCACTGCAAATCCTTA
AGCAAGCCAAACTTCAAGGCATGCGCGACTTACGAGATTCAGGCCTGCTAAAAGTCATCCAAGGCGTCACCAGTATTGCA
GAAATAAACCGCGTCACTAGCTTTTAA

Upstream 100 bases:

>100_bases
GCAACTGCTGATGATTTAGAGCTTTGTGAAAAAGCAGCGGGTCTATATGTAGCACCATCAGTATAAAAGGTTACATTCAG
TAGCTGTATACTAATATTCA

Downstream 100 bases:

>100_bases
TATCCCCAACTTTAACTACTGCACCTAAATATCAAAAGGATACGTTATGGCGACGGCAACAATTAAACCTAAAAAGACTC
GCGCCAAGAAAACGAAAAAC

Product: type IV-A pilus assembly ATPase PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MPTTGLHLGLSTLFIRKGLLNEKQLSEAVSKSRKLRQGLVATLVKDNLISAREIAELCYEEYGTPLLDLNEFEIGSIPEE
FLNKKLIEKHKCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEDDKLSKAIDSILEDDISGLDLSAIDEEA
LAGIEVTDTDKRDEEENSGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRLLYEEMLQKPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLSRRLCPTCKAPEAIPAEELLKLGFN
QQQVDAGIIPHKPVGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQILKQAKLQGMRDLRDSGLLKVIQGVTSIA
EINRVTSF

Sequences:

>Translated_568_residues
MPTTGLHLGLSTLFIRKGLLNEKQLSEAVSKSRKLRQGLVATLVKDNLISAREIAELCYEEYGTPLLDLNEFEIGSIPEE
FLNKKLIEKHKCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEDDKLSKAIDSILEDDISGLDLSAIDEEA
LAGIEVTDTDKRDEEENSGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRLLYEEMLQKPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLSRRLCPTCKAPEAIPAEELLKLGFN
QQQVDAGIIPHKPVGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQILKQAKLQGMRDLRDSGLLKVIQGVTSIA
EINRVTSF
>Mature_567_residues
PTTGLHLGLSTLFIRKGLLNEKQLSEAVSKSRKLRQGLVATLVKDNLISAREIAELCYEEYGTPLLDLNEFEIGSIPEEF
LNKKLIEKHKCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEDDKLSKAIDSILEDDISGLDLSAIDEEAL
AGIEVTDTDKRDEEENSGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSGRISARL
KVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRLLYEEMLQKPQG
MILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLSRRLCPTCKAPEAIPAEELLKLGFNQ
QQVDAGIIPHKPVGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQILKQAKLQGMRDLRDSGLLKVIQGVTSIAE
INRVTSF

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=458, Percent_Identity=44.7598253275109, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1786296, Length=378, Percent_Identity=46.8253968253968, Blast_Score=339, Evalue=3e-94,
Organism=Escherichia coli, GI87082188, Length=281, Percent_Identity=33.8078291814947, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62801; Mature: 62670

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTTGLHLGLSTLFIRKGLLNEKQLSEAVSKSRKLRQGLVATLVKDNLISAREIAELCYE
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
EYGTPLLDLNEFEIGSIPEEFLNKKLIEKHKCLPLFKRGNRLYIATSDPTNIAALEDFQF
HHCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCEEEEECCCC
SAGLHAEAILVEDDKLSKAIDSILEDDISGLDLSAIDEEALAGIEVTDTDKRDEEENSGK
CCCCCEEEEEEECHHHHHHHHHHHHHCCCCCCCHHCCHHHHCCCEEECCCCCCCHHCCCC
DDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSGRISAR
CCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHHHHHCCCCCCCCCCCHHHH
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIE
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCHHCCHH
KLGYEDDQRLLYEEMLQKPQGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEI
HCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCEEECCCCCCCCCCCCCEEE
NLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRDLETAEIAIKAAQTGHLVLST
EEECCEEEEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECCCCCEEEEE
LHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLSRRLCPTCKAPEAIPAEELLKLGFN
ECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCC
QQQVDAGIIPHKPVGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQILKQAKLQG
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MRDLRDSGLLKVIQGVTSIAEINRVTSF
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTTGLHLGLSTLFIRKGLLNEKQLSEAVSKSRKLRQGLVATLVKDNLISAREIAELCYE
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
EYGTPLLDLNEFEIGSIPEEFLNKKLIEKHKCLPLFKRGNRLYIATSDPTNIAALEDFQF
HHCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCEEEEECCCC
SAGLHAEAILVEDDKLSKAIDSILEDDISGLDLSAIDEEALAGIEVTDTDKRDEEENSGK
CCCCCEEEEEEECHHHHHHHHHHHHHCCCCCCCHHCCHHHHCCCEEECCCCCCCHHCCCC
DDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSGRISAR
CCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHHHHHCCCCCCCCCCCHHHH
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIE
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCHHCCHH
KLGYEDDQRLLYEEMLQKPQGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEI
HCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCEEECCCCCCCCCCCCCEEE
NLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRDLETAEIAIKAAQTGHLVLST
EEECCEEEEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECCCCCEEEEE
LHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLSRRLCPTCKAPEAIPAEELLKLGFN
ECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCC
QQQVDAGIIPHKPVGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQILKQAKLQG
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MRDLRDSGLLKVIQGVTSIAEINRVTSF
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]