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The map label for this gene is rho [H]

Identifier: 157960226

GI number: 157960226

Start: 479324

End: 480589

Strand: Reverse

Name: rho [H]

Synonym: Spea_0397

Alternate gene names: 157960226

Gene position: 480589-479324 (Counterclockwise)

Preceding gene: 157960227

Following gene: 157960220

Centisome position: 9.29

GC content: 43.84

Gene sequence:

>1266_bases
ATGAATTTATCAGAATTAAAAGACACCTCGATTTCAGATTTAGTCCAGCTTGCTCAAGACATGAAGCTAGAAAATATGGC
TCGTGCTCGTAAGCAAGACATTATTTTCTCTATCCTTAAGGCCCACGCTAAAAGCGGTGAAGATATTTTCGGTGGTGGCG
TTTTAGAGATCTTACAAGATGGCTTCGGCTTCCTTCGTAGCTCTGATGGTTCATACTTAGCGGGCCCAGATGATATCTAC
GTTTCACCTAGCCAAATTCGACGCTTTAACATGCGCACTGGTGACACGATTTTCGGTAAAATCCGACCACCAAAAGAAGG
CGAGCGTTATTTTGCCCTTCTAAAAGTGACCGAAGTTAACTTCGACAAGCCTGAAAACTCTCGCAACAAGATCCTATTTG
AAAACTTAACACCTCTACATGCAGAAGAACGTATGCGTATGGAACGTGGTAATGGTTCAACTGAAGACATCACTTCACGT
ATTCTGGATTTATGCTCACCAATTGGTAAAGGTCAACGTGGTCTTATCGTTGCGCCACCAAAAGCGGGTAAAACCTTATT
GCTTCAAACTATGGCGCAAAGTATCACCTACAACAATCCAGAAGTGGTGTTGATGGTACTACTTATCGACGAACGTCCTG
AAGAAGTTACCGAGATGCAACGTATGGTAAAAGGTGAAGTTATTGCTTCAACTTTCGACGAGCCAGCAAGCCGTCACGTA
CAAGTTGCAGAAATGGTAATTGAAAAGGCTAAACGTTTAGTAGAGCATAAGAAAGACGTCGTAATCTTACTTGATTCTAT
TACTCGTCTTGCTCGTGCATACAACACTGTTATTCCTTCATCTGGCAAGGTGCTTACAGGTGGTGTTGACGCTAACGCAC
TACACCGTCCAAAGCGTTTCTTCGGTGCAGCTCGTAACATCGAACATGGTGGTAGCTTAACCATTATCGCTACTGCGCTT
GTTGATACAGGCTCTAAGATGGACGAAGTGATTTACGAAGAATTTAAAGGTACTGGTAACCAAGAACTACACCTTTCTCG
TAAAGCCGCTGAGAAGCGAGTGTTCCCTGCAATCGACTTTAACCGCTCTGGTACCCGTCGTGAAGAGAAGCTGACTACAC
CGGATGAACTACAGAAGATGTGGATCCTACGTAAGATCCTTAATCCGATGGATGAAGTGACAGGTATGGAATTCCTTATC
GACAAGTTGGCAATGACCAAGACAAACGATGAGTTCTTCACTGCGATGAAGCGCGCTAAGAGCTAA

Upstream 100 bases:

>100_bases
TTCTAATTAACCCACTTCTCGCTATCCGATCGATATTCAATAAGCTACAAAACCTTTGATCGTAGATGGCATTGCCACGT
ACAAAACAAGACCCACCACG

Downstream 100 bases:

>100_bases
CTTAGGCGTCATTGATAAAAAAACCGCACTCGTGCGGTTTTTTTGTGTCTGCTATTTGATCACTCCATCAAGTGACTCAC
CCGCGCTAAACGTTGTTTAC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MNLSELKDTSISDLVQLAQDMKLENMARARKQDIIFSILKAHAKSGEDIFGGGVLEILQDGFGFLRSSDGSYLAGPDDIY
VSPSQIRRFNMRTGDTIFGKIRPPKEGERYFALLKVTEVNFDKPENSRNKILFENLTPLHAEERMRMERGNGSTEDITSR
ILDLCSPIGKGQRGLIVAPPKAGKTLLLQTMAQSITYNNPEVVLMVLLIDERPEEVTEMQRMVKGEVIASTFDEPASRHV
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDANALHRPKRFFGAARNIEHGGSLTIIATAL
VDTGSKMDEVIYEEFKGTGNQELHLSRKAAEKRVFPAIDFNRSGTRREEKLTTPDELQKMWILRKILNPMDEVTGMEFLI
DKLAMTKTNDEFFTAMKRAKS

Sequences:

>Translated_421_residues
MNLSELKDTSISDLVQLAQDMKLENMARARKQDIIFSILKAHAKSGEDIFGGGVLEILQDGFGFLRSSDGSYLAGPDDIY
VSPSQIRRFNMRTGDTIFGKIRPPKEGERYFALLKVTEVNFDKPENSRNKILFENLTPLHAEERMRMERGNGSTEDITSR
ILDLCSPIGKGQRGLIVAPPKAGKTLLLQTMAQSITYNNPEVVLMVLLIDERPEEVTEMQRMVKGEVIASTFDEPASRHV
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDANALHRPKRFFGAARNIEHGGSLTIIATAL
VDTGSKMDEVIYEEFKGTGNQELHLSRKAAEKRVFPAIDFNRSGTRREEKLTTPDELQKMWILRKILNPMDEVTGMEFLI
DKLAMTKTNDEFFTAMKRAKS
>Mature_421_residues
MNLSELKDTSISDLVQLAQDMKLENMARARKQDIIFSILKAHAKSGEDIFGGGVLEILQDGFGFLRSSDGSYLAGPDDIY
VSPSQIRRFNMRTGDTIFGKIRPPKEGERYFALLKVTEVNFDKPENSRNKILFENLTPLHAEERMRMERGNGSTEDITSR
ILDLCSPIGKGQRGLIVAPPKAGKTLLLQTMAQSITYNNPEVVLMVLLIDERPEEVTEMQRMVKGEVIASTFDEPASRHV
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDANALHRPKRFFGAARNIEHGGSLTIIATAL
VDTGSKMDEVIYEEFKGTGNQELHLSRKAAEKRVFPAIDFNRSGTRREEKLTTPDELQKMWILRKILNPMDEVTGMEFLI
DKLAMTKTNDEFFTAMKRAKS

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=419, Percent_Identity=84.964200477327, Blast_Score=751, Evalue=0.0,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47231; Mature: 47231

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSELKDTSISDLVQLAQDMKLENMARARKQDIIFSILKAHAKSGEDIFGGGVLEILQD
CCHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
GFGFLRSSDGSYLAGPDDIYVSPSQIRRFNMRTGDTIFGKIRPPKEGERYFALLKVTEVN
HHHHHCCCCCCEECCCCCEEECHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEEEEECC
FDKPENSRNKILFENLTPLHAEERMRMERGNGSTEDITSRILDLCSPIGKGQRGLIVAPP
CCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECC
KAGKTLLLQTMAQSITYNNPEVVLMVLLIDERPEEVTEMQRMVKGEVIASTFDEPASRHV
CCCHHHHHHHHHHHHCCCCHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDANALHRPKRF
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHCCHHH
FGAARNIEHGGSLTIIATALVDTGSKMDEVIYEEFKGTGNQELHLSRKAAEKRVFPAIDF
HHHHCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCC
NRSGTRREEKLTTPDELQKMWILRKILNPMDEVTGMEFLIDKLAMTKTNDEFFTAMKRAK
CCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
S
C
>Mature Secondary Structure
MNLSELKDTSISDLVQLAQDMKLENMARARKQDIIFSILKAHAKSGEDIFGGGVLEILQD
CCHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
GFGFLRSSDGSYLAGPDDIYVSPSQIRRFNMRTGDTIFGKIRPPKEGERYFALLKVTEVN
HHHHHCCCCCCEECCCCCEEECHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEEEEECC
FDKPENSRNKILFENLTPLHAEERMRMERGNGSTEDITSRILDLCSPIGKGQRGLIVAPP
CCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECC
KAGKTLLLQTMAQSITYNNPEVVLMVLLIDERPEEVTEMQRMVKGEVIASTFDEPASRHV
CCCHHHHHHHHHHHHCCCCHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDANALHRPKRF
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHCCHHH
FGAARNIEHGGSLTIIATALVDTGSKMDEVIYEEFKGTGNQELHLSRKAAEKRVFPAIDF
HHHHCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCC
NRSGTRREEKLTTPDELQKMWILRKILNPMDEVTGMEFLIDKLAMTKTNDEFFTAMKRAK
CCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]