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The map label for this gene is frdB [H]

Identifier: 157960224

GI number: 157960224

Start: 477474

End: 478217

Strand: Direct

Name: frdB [H]

Synonym: Spea_0395

Alternate gene names: 157960224

Gene position: 477474-478217 (Clockwise)

Preceding gene: 157960223

Following gene: 157960225

Centisome position: 9.23

GC content: 46.91

Gene sequence:

>744_bases
ATGAGCCAACAAATGGCTAAGGGCCGTACCTTAACTTTCAGTATTTTTAGATTTGACCCACAAGTGCCGGGTGATAAACC
TAAAATGGTTGAGTACCAGATCGAAGAAGCCCCCGGTATGACGGTCTTTATTGCACTCAATCAGTTGCGTGAGTTACAAG
ATCCATCGTTGCAGTTCGATTTCGTTTGTCGTGCAGGTATTTGCGGTAGCTGCGCCATGGTGATTAACGGTATGCCAACT
TTGGCCTGCCGTACGCTAACCAGTAAATTTACTGATGGTAAAATCAAGTTAATGCCACTACCTGGCTTTGAGCTGATTGG
CGATTTATCGGTAAATACTGGTAAGTTTATGCGTGAAATCGCTGAACGCTTGAAGCTGTGGTTACACCCAAAAGTGGGTG
ATGATGATATTCATCGTCTTGAGGCGCCAATGTCACCTGAGGAGGCGGCTAAACTTTATGAGTTAGAGCGCTGCGTCGAG
TGTGGTGTATGTGTTTCAGCTTGTGCCACCAAGCAGATGCGTGACACCTTTGTGGGGGCCGTAGGCCTAATGAAGATTGC
CCGTTTTGAGTTAGATAGTCGTGACGCGCGTAATGCTGAAGACTTCTACCACGTGATAGGTAATCAAGACGGCGTATTTG
GCTGTATGACGCTGCTAGGCTGCCAAGATAACTGCCCTAAAGATTTACCGCATATGCAGCAGATTGCTTATTTACGCCGT
AAGATGGCGGTAACGCTAGTCTGA

Upstream 100 bases:

>100_bases
AAGCAGCAGTCATGCCGTTTGACGTACCTGACTCATTAAAACCAAAGAACGAACGTTTGAGTGACACATTAGCGCACTCG
AGCACAGGAGAGATCGCTAA

Downstream 100 bases:

>100_bases
GTTGCAACGCATTAGTTAAAGTGACGATTTAAAGAGCCTGCATTGCGCAGGCTTTTTTGTGGGTGACGTTTAGTCTCTGT
TTATCTACAATTTGATTTAA

Product: fumarate reductase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSQQMAKGRTLTFSIFRFDPQVPGDKPKMVEYQIEEAPGMTVFIALNQLRELQDPSLQFDFVCRAGICGSCAMVINGMPT
LACRTLTSKFTDGKIKLMPLPGFELIGDLSVNTGKFMREIAERLKLWLHPKVGDDDIHRLEAPMSPEEAAKLYELERCVE
CGVCVSACATKQMRDTFVGAVGLMKIARFELDSRDARNAEDFYHVIGNQDGVFGCMTLLGCQDNCPKDLPHMQQIAYLRR
KMAVTLV

Sequences:

>Translated_247_residues
MSQQMAKGRTLTFSIFRFDPQVPGDKPKMVEYQIEEAPGMTVFIALNQLRELQDPSLQFDFVCRAGICGSCAMVINGMPT
LACRTLTSKFTDGKIKLMPLPGFELIGDLSVNTGKFMREIAERLKLWLHPKVGDDDIHRLEAPMSPEEAAKLYELERCVE
CGVCVSACATKQMRDTFVGAVGLMKIARFELDSRDARNAEDFYHVIGNQDGVFGCMTLLGCQDNCPKDLPHMQQIAYLRR
KMAVTLV
>Mature_246_residues
SQQMAKGRTLTFSIFRFDPQVPGDKPKMVEYQIEEAPGMTVFIALNQLRELQDPSLQFDFVCRAGICGSCAMVINGMPTL
ACRTLTSKFTDGKIKLMPLPGFELIGDLSVNTGKFMREIAERLKLWLHPKVGDDDIHRLEAPMSPEEAAKLYELERCVEC
GVCVSACATKQMRDTFVGAVGLMKIARFELDSRDARNAEDFYHVIGNQDGVFGCMTLLGCQDNCPKDLPHMQQIAYLRRK
MAVTLV

Specific function: The fumarate reductase enzyme complex is required for fumarate respiration using formate or sulfide as electron donor [H]

COG id: COG0479

COG function: function code C; Succinate dehydrogenase/fumarate reductase, Fe-S protein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI115387094, Length=240, Percent_Identity=32.9166666666667, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1786943, Length=234, Percent_Identity=35.8974358974359, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1790596, Length=227, Percent_Identity=28.1938325991189, Blast_Score=114, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17533915, Length=238, Percent_Identity=31.5126050420168, Blast_Score=124, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6322987, Length=240, Percent_Identity=31.25, Blast_Score=116, Evalue=4e-27,
Organism=Drosophila melanogaster, GI17137106, Length=234, Percent_Identity=31.6239316239316, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24643156, Length=236, Percent_Identity=29.6610169491525, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012675
- InterPro:   IPR001041
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR004489 [H]

Pfam domain/function: NA

EC number: =1.3.99.1 [H]

Molecular weight: Translated: 27654; Mature: 27523

Theoretical pI: Translated: 6.49; Mature: 6.49

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
10.1 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
9.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQMAKGRTLTFSIFRFDPQVPGDKPKMVEYQIEEAPGMTVFIALNQLRELQDPSLQFD
CCCCHHCCCEEEEEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEEHHHHHCCCCCCEEE
FVCRAGICGSCAMVINGMPTLACRTLTSKFTDGKIKLMPLPGFELIGDLSVNTGKFMREI
HEECCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCHHHEECCCCCHHHHHHHH
AERLKLWLHPKVGDDDIHRLEAPMSPEEAAKLYELERCVECGVCVSACATKQMRDTFVGA
HHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGLMKIARFELDSRDARNAEDFYHVIGNQDGVFGCMTLLGCQDNCPKDLPHMQQIAYLRR
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
KMAVTLV
HHHHCCC
>Mature Secondary Structure 
SQQMAKGRTLTFSIFRFDPQVPGDKPKMVEYQIEEAPGMTVFIALNQLRELQDPSLQFD
CCCHHCCCEEEEEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEEHHHHHCCCCCCEEE
FVCRAGICGSCAMVINGMPTLACRTLTSKFTDGKIKLMPLPGFELIGDLSVNTGKFMREI
HEECCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCHHHEECCCCCHHHHHHHH
AERLKLWLHPKVGDDDIHRLEAPMSPEEAAKLYELERCVECGVCVSACATKQMRDTFVGA
HHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGLMKIARFELDSRDARNAEDFYHVIGNQDGVFGCMTLLGCQDNCPKDLPHMQQIAYLRR
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
KMAVTLV
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2244791; 9492313; 14500908; 10586875; 11248702 [H]