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The map label for this gene is frdA [H]

Identifier: 157960223

GI number: 157960223

Start: 475477

End: 477477

Strand: Direct

Name: frdA [H]

Synonym: Spea_0394

Alternate gene names: 157960223

Gene position: 475477-477477 (Clockwise)

Preceding gene: 157960222

Following gene: 157960224

Centisome position: 9.19

GC content: 48.93

Gene sequence:

>2001_bases
GTGAAACTGATATATACCGATTCGTTAGTGATCGGCGCTGGACTTGCTGGATTACGAGTGGCTATCGCGTCTAAAGAGCG
TGGGCTAGATACTGTTGTATTATCATTGATCCCAGCGAAACGCTCGCATTCAGCGGCAGCGCAGGGGGGCATGCAAGCAA
GTTTGGGCAATACCGTTAAAGGTATGGGAGATGACGAAGATGTGCATTTCCAAGATACCGTAAAAGGATCTGACTGGGGA
TGCGATCAAGATGTGGCACGTATGTTTGCCCACTGTGCCCCTAAAGCCGTGCGAGAATTGGCAAACTGGGGTGTACCTTG
GACGCGCATAACCAAAGGTCCGCGTCAGGTGGTGGTTAATGCTGAAAAGGTGACCATTGAAGAAGCAGAAGAGGCGCATG
GTTTAATTAACGCCCGTGACTTTGGGGGCACCAAAAAATGGCGCACTTGTTACACCGCTGACGGCACAGGCCACTCGCTA
CTTTATGCGGTAGATAACAAGGCTATTTCAATGGACATTCCAGTCCATGAGCGTATGGAGGCGTTATCGCTTATTCATGA
TGGCAAGCGTTGTCATGGTGTGGTTGCTCGCTGCCTAATTACTGGCGAGCTAATCGCTTACGTTGCAAAGTCGACCACGA
TTGCAACTGGTGGCTATGGTCGTATTTATGAAGTATCGACTAACGCCATTATTTGTGAAGGTGTAGGCCAGGCGCTAGCA
CTTGAAACCGGTGTTGCCAAGCTAGGTAACATGGAAGCGGTGCAGTTCCATCCTACGGCTATTGTGCCTGTCGGTATTTT
GACTACTGAAGGTTGTCGTGGTGACGGCGGTTTATTGCGTGATAAAGATGGCCATCGCTTTATGCCAGATTACGAGCCAG
AGAAGAAAGAGCTGGCATCACGTGACGTAGTATCGCGCCGCATGACAGAACACATGCGCAAAGGTAAGGGCGTAGACAGT
CCTTATGGGCCGCACTTATGGCTGGATATTACTTTGCTTGGTCGTAAACACGTAGAAACCAACCTACGTGAAGTAAAAGA
GATCTGTGAGAACTTTCTTGGCATCGACCCAGCTAAAGACTGGATCCCAGTAAGACCGACTCAGCACTATTCTATGGGCG
GTATTCGTACCGACGCTACGGGCCAAAGCCCACAGCTAAAAGGTTTATTCAGTGTCGGCGAAGCGGCTTGTTGGGATATG
CATGGCTTTAACCGCTTAGGTGGTAACTCACTCGCTGAGACAGTAGTTGGCGGTATGATTATCGGTAAGTATGTCGCCGA
TTTTTGTGAACAAAACACCCTTGAAATCGATACCAGCTTAGTTGAGTCCTTTGTAGGTAAACTACAAAGCGAGATTGACG
AGCTTATTGATGGTGAAGGCACCGAAAATCCATTTGAGCTTAAGCAGCAGATGCAGCAGATCATGATGGATTACGTGGGG
ATTTTCCGTAATGGTCCTGAGCTTGAAAAAGCGGTTAGCGAGCTACAAGACTTACTTAAGCGTGCAAATGCATTAGGACT
TAAGTGCAAGAAGCGGCATGCTAACCCTGAGCTTGTTGAAGCACTGCGTGTGAAGCGTATGCTTAAAGTGGCACTGACGG
TTGCAGCTGGCGCTGCAGCACGTACAGAGAGCCGCGGGGCGCACGCTCGTGAAGACTTCCCACAGCGTAACGACAAAGAC
TGGCTAAACCGCACATTATCAAGCTGGCCGGATGCTAATAGCCTGTCACCTCAACTTGAGTATGAACAGCTAGATGTGAT
GAAGATGGAGTTGCCACCAGGTTACCGTGGTTACGGTATCGATAATGCCATTGCGCATCCAGATACCGAAAAACGTGAGC
AAGAAATTGCAGAGATTATTAGTCAGCTTGGTCCTGATGCAGACAGGCATCAACAACAAGCAGCAGTCATGCCGTTTGAC
GTACCTGACTCATTAAAACCAAAGAACGAACGTTTGAGTGACACATTAGCGCACTCGAGCACAGGAGAGATCGCTAAATG
A

Upstream 100 bases:

>100_bases
GCTTGGCGTCCTTATTGGCGTTCCTCCCCTATGCCAATGGGTAGCTGCTGGTGAGAACAAGAACATAAACTGACTCTGCA
CCCTACGTTAAGGAGCACGT

Downstream 100 bases:

>100_bases
GCCAACAAATGGCTAAGGGCCGTACCTTAACTTTCAGTATTTTTAGATTTGACCCACAAGTGCCGGGTGATAAACCTAAA
ATGGTTGAGTACCAGATCGA

Product: fumarate reductase flavoprotein subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 666; Mature: 666

Protein sequence:

>666_residues
MKLIYTDSLVIGAGLAGLRVAIASKERGLDTVVLSLIPAKRSHSAAAQGGMQASLGNTVKGMGDDEDVHFQDTVKGSDWG
CDQDVARMFAHCAPKAVRELANWGVPWTRITKGPRQVVVNAEKVTIEEAEEAHGLINARDFGGTKKWRTCYTADGTGHSL
LYAVDNKAISMDIPVHERMEALSLIHDGKRCHGVVARCLITGELIAYVAKSTTIATGGYGRIYEVSTNAIICEGVGQALA
LETGVAKLGNMEAVQFHPTAIVPVGILTTEGCRGDGGLLRDKDGHRFMPDYEPEKKELASRDVVSRRMTEHMRKGKGVDS
PYGPHLWLDITLLGRKHVETNLREVKEICENFLGIDPAKDWIPVRPTQHYSMGGIRTDATGQSPQLKGLFSVGEAACWDM
HGFNRLGGNSLAETVVGGMIIGKYVADFCEQNTLEIDTSLVESFVGKLQSEIDELIDGEGTENPFELKQQMQQIMMDYVG
IFRNGPELEKAVSELQDLLKRANALGLKCKKRHANPELVEALRVKRMLKVALTVAAGAAARTESRGAHAREDFPQRNDKD
WLNRTLSSWPDANSLSPQLEYEQLDVMKMELPPGYRGYGIDNAIAHPDTEKREQEIAEIISQLGPDADRHQQQAAVMPFD
VPDSLKPKNERLSDTLAHSSTGEIAK

Sequences:

>Translated_666_residues
MKLIYTDSLVIGAGLAGLRVAIASKERGLDTVVLSLIPAKRSHSAAAQGGMQASLGNTVKGMGDDEDVHFQDTVKGSDWG
CDQDVARMFAHCAPKAVRELANWGVPWTRITKGPRQVVVNAEKVTIEEAEEAHGLINARDFGGTKKWRTCYTADGTGHSL
LYAVDNKAISMDIPVHERMEALSLIHDGKRCHGVVARCLITGELIAYVAKSTTIATGGYGRIYEVSTNAIICEGVGQALA
LETGVAKLGNMEAVQFHPTAIVPVGILTTEGCRGDGGLLRDKDGHRFMPDYEPEKKELASRDVVSRRMTEHMRKGKGVDS
PYGPHLWLDITLLGRKHVETNLREVKEICENFLGIDPAKDWIPVRPTQHYSMGGIRTDATGQSPQLKGLFSVGEAACWDM
HGFNRLGGNSLAETVVGGMIIGKYVADFCEQNTLEIDTSLVESFVGKLQSEIDELIDGEGTENPFELKQQMQQIMMDYVG
IFRNGPELEKAVSELQDLLKRANALGLKCKKRHANPELVEALRVKRMLKVALTVAAGAAARTESRGAHAREDFPQRNDKD
WLNRTLSSWPDANSLSPQLEYEQLDVMKMELPPGYRGYGIDNAIAHPDTEKREQEIAEIISQLGPDADRHQQQAAVMPFD
VPDSLKPKNERLSDTLAHSSTGEIAK
>Mature_666_residues
MKLIYTDSLVIGAGLAGLRVAIASKERGLDTVVLSLIPAKRSHSAAAQGGMQASLGNTVKGMGDDEDVHFQDTVKGSDWG
CDQDVARMFAHCAPKAVRELANWGVPWTRITKGPRQVVVNAEKVTIEEAEEAHGLINARDFGGTKKWRTCYTADGTGHSL
LYAVDNKAISMDIPVHERMEALSLIHDGKRCHGVVARCLITGELIAYVAKSTTIATGGYGRIYEVSTNAIICEGVGQALA
LETGVAKLGNMEAVQFHPTAIVPVGILTTEGCRGDGGLLRDKDGHRFMPDYEPEKKELASRDVVSRRMTEHMRKGKGVDS
PYGPHLWLDITLLGRKHVETNLREVKEICENFLGIDPAKDWIPVRPTQHYSMGGIRTDATGQSPQLKGLFSVGEAACWDM
HGFNRLGGNSLAETVVGGMIIGKYVADFCEQNTLEIDTSLVESFVGKLQSEIDELIDGEGTENPFELKQQMQQIMMDYVG
IFRNGPELEKAVSELQDLLKRANALGLKCKKRHANPELVEALRVKRMLKVALTVAAGAAARTESRGAHAREDFPQRNDKD
WLNRTLSSWPDANSLSPQLEYEQLDVMKMELPPGYRGYGIDNAIAHPDTEKREQEIAEIISQLGPDADRHQQQAAVMPFD
VPDSLKPKNERLSDTLAHSSTGEIAK

Specific function: The fumarate reductase enzyme complex is required for fumarate respiration using formate or sulfide as electron donor [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=607, Percent_Identity=35.9143327841845, Blast_Score=325, Evalue=7e-89,
Organism=Escherichia coli, GI1786942, Length=604, Percent_Identity=37.2516556291391, Blast_Score=336, Evalue=3e-93,
Organism=Escherichia coli, GI1790597, Length=583, Percent_Identity=35.6775300171527, Blast_Score=324, Evalue=1e-89,
Organism=Escherichia coli, GI1788928, Length=570, Percent_Identity=29.4736842105263, Blast_Score=184, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17505833, Length=550, Percent_Identity=38.7272727272727, Blast_Score=329, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI17550100, Length=546, Percent_Identity=38.2783882783883, Blast_Score=317, Evalue=9e-87,
Organism=Saccharomyces cerevisiae, GI6322701, Length=598, Percent_Identity=36.1204013377926, Blast_Score=331, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6322416, Length=590, Percent_Identity=35.4237288135593, Blast_Score=324, Evalue=3e-89,
Organism=Drosophila melanogaster, GI17137288, Length=603, Percent_Identity=36.318407960199, Blast_Score=331, Evalue=9e-91,
Organism=Drosophila melanogaster, GI24655642, Length=603, Percent_Identity=36.318407960199, Blast_Score=331, Evalue=9e-91,
Organism=Drosophila melanogaster, GI24655647, Length=603, Percent_Identity=36.318407960199, Blast_Score=331, Evalue=9e-91,
Organism=Drosophila melanogaster, GI24663005, Length=573, Percent_Identity=34.2059336823735, Blast_Score=272, Evalue=5e-73,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR003952
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR014006 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.3.99.1 [H]

Molecular weight: Translated: 72995; Mature: 72995

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00504 FRD_SDH_FAD_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIYTDSLVIGAGLAGLRVAIASKERGLDTVVLSLIPAKRSHSAAAQGGMQASLGNTVK
CEEEEECCHHHHCCHHHHEEEECCCCCCHHHHHHHHHCCCCCCHHHHHCCCHHHCCCCCC
GMGDDEDVHFQDTVKGSDWGCDQDVARMFAHCAPKAVRELANWGVPWTRITKGPRQVVVN
CCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCHHHHCCCCHHEEEE
AEKVTIEEAEEAHGLINARDFGGTKKWRTCYTADGTGHSLLYAVDNKAISMDIPVHERME
CHHEEHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCEEEEECCHHHHHH
ALSLIHDGKRCHGVVARCLITGELIAYVAKSTTIATGGYGRIYEVSTNAIICEGVGQALA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEECCEEEECCCCCHHH
LETGVAKLGNMEAVQFHPTAIVPVGILTTEGCRGDGGLLRDKDGHRFMPDYEPEKKELAS
HHHHHHHHCCCCEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RDVVSRRMTEHMRKGKGVDSPYGPHLWLDITLLGRKHVETNLREVKEICENFLGIDPAKD
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCC
WIPVRPTQHYSMGGIRTDATGQSPQLKGLFSVGEAACWDMHGFNRLGGNSLAETVVGGMI
CCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHCCHHHHCCCCCHHCCCHHHHHHHHHHHH
IGKYVADFCEQNTLEIDTSLVESFVGKLQSEIDELIDGEGTENPFELKQQMQQIMMDYVG
HHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
IFRNGPELEKAVSELQDLLKRANALGLKCKKRHANPELVEALRVKRMLKVALTVAAGAAA
HHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
RTESRGAHAREDFPQRNDKDWLNRTLSSWPDANSLSPQLEYEQLDVMKMELPPGYRGYGI
HCCCCCCCCHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHCEEEEECCCCCCCCCC
DNAIAHPDTEKREQEIAEIISQLGPDADRHQQQAAVMPFDVPDSLKPKNERLSDTLAHSS
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCC
TGEIAK
CCCCCC
>Mature Secondary Structure
MKLIYTDSLVIGAGLAGLRVAIASKERGLDTVVLSLIPAKRSHSAAAQGGMQASLGNTVK
CEEEEECCHHHHCCHHHHEEEECCCCCCHHHHHHHHHCCCCCCHHHHHCCCHHHCCCCCC
GMGDDEDVHFQDTVKGSDWGCDQDVARMFAHCAPKAVRELANWGVPWTRITKGPRQVVVN
CCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCHHHHCCCCHHEEEE
AEKVTIEEAEEAHGLINARDFGGTKKWRTCYTADGTGHSLLYAVDNKAISMDIPVHERME
CHHEEHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCEEEEECCHHHHHH
ALSLIHDGKRCHGVVARCLITGELIAYVAKSTTIATGGYGRIYEVSTNAIICEGVGQALA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEECCEEEECCCCCHHH
LETGVAKLGNMEAVQFHPTAIVPVGILTTEGCRGDGGLLRDKDGHRFMPDYEPEKKELAS
HHHHHHHHCCCCEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RDVVSRRMTEHMRKGKGVDSPYGPHLWLDITLLGRKHVETNLREVKEICENFLGIDPAKD
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCC
WIPVRPTQHYSMGGIRTDATGQSPQLKGLFSVGEAACWDMHGFNRLGGNSLAETVVGGMI
CCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHCCHHHHCCCCCHHCCCHHHHHHHHHHHH
IGKYVADFCEQNTLEIDTSLVESFVGKLQSEIDELIDGEGTENPFELKQQMQQIMMDYVG
HHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
IFRNGPELEKAVSELQDLLKRANALGLKCKKRHANPELVEALRVKRMLKVALTVAAGAAA
HHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
RTESRGAHAREDFPQRNDKDWLNRTLSSWPDANSLSPQLEYEQLDVMKMELPPGYRGYGI
HCCCCCCCCHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHCEEEEECCCCCCCCCC
DNAIAHPDTEKREQEIAEIISQLGPDADRHQQQAAVMPFDVPDSLKPKNERLSDTLAHSS
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCC
TGEIAK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2244791; 9492313; 14500908; 3609021; 10586875; 11248702 [H]