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The map label for this gene is ilvD [H]

Identifier: 157960177

GI number: 157960177

Start: 394506

End: 396353

Strand: Direct

Name: ilvD [H]

Synonym: Spea_0348

Alternate gene names: 157960177

Gene position: 394506-396353 (Clockwise)

Preceding gene: 157960176

Following gene: 157960178

Centisome position: 7.62

GC content: 51.89

Gene sequence:

>1848_bases
ATGCCAAAACTACGTTCAGCAACCAGTACCGAAGGCCGTAACATGGCGGGAGCGCGTGCGCTATGGCGTGCAACAGGTGT
GAAAGATAACGACTTCGGTAAGCCGATTATCGCAATTTCAAACTCGTTCACTCAGTTTGTGCCGGGGCACGTTCACCTGA
AAGATATGGGGTCTTTGGTGGCGGGAGCGATCGAGGAAGCGGGCGGCATTGCTAAAGAATTTAATACCATTGCAGTGGAT
GACGGTATCGCTATGGGACACGGCGGTATGCTTTATAGCTTACCATCGCGCGAGCTGATTGCAGATAGTGTGGAGTATAT
GGTCAATGCCCACTGTGCCGATGCACTAGTGTGTATTTCTAACTGCGATAAGATCACCCCTGGCATGCTCATGGCCTCAC
TTAGACTGAATATCCCTGTCATCTTTGTCTCAGGTGGCCCGATGGAAGCGGGTAAAACTAAGCTGTCTGATAAGTTAATT
AAGCTGGACTTAGTCGATGCCATGGTGGCCGGCGCCGATGAGCGCGTATCCGATGCCGATAGCGAGCAGATTGAGCGCAG
CGCCTGCCCAACTTGTGGCTCATGTTCGGGTATGTTTACTGCTAACTCGATGAACTGCTTGACCGAAGCACTAGGCCTTT
CACTGCCGGGTAACGGATCTATGCTGGCCACCCACGCCGATCGTCGCGAACTCTTCCTCGAAGCGGGTCGTCGCATCATG
GATTTAGCCACTCGTTATTATAAGCATGATGACGAGTCAGCATTGCCACGTAATATCGCTAACTTCAAGGCGTTTGAAAA
TGCCATGACGCTAGATATCGCCATGGGCGGATCAAGCAATACCGTGCTGCATTTACTTGCAGCAGCGCAAGAAGCTGAAG
TCGATTTCACCATGGATGATATCGACCGTCTGTCGCGCCTTGTACCGCACCTTTGTAAGGTGGCTCCGGCAACGCCTAAA
TACCATATGGAAGATGTCCACCGCGCTGGTGGTGTAATGGGGATTCTAGGTGAGCTAGACCGAGCTAACTTACTGCATAA
CGACGCCTACCACGTAGCGGGTGAAAACCTTGCCGCAGTATTGGCAAAATACGATATCGCACAGAGTGATGACGCCGCTG
TGCGTAAATTCTTCTCTGCAGGCCCTGCTGGTATTCCAACTACCAAGGCCTTTAGTCAGGATTGCCGCTGGGACAGCGTC
GATGATGACCGTCAGCAAGGTTGTATCCGTAGCCGTGAATTTGCCTTTAGCCAAGAGGGCGGGCTTGCCGTGTTATCGGG
TAACGTGGCCGTTGATGGTTGTATCGTTAAGACTGCTGGCGTAGAGGTAGAAAATCACACCTTTATCGGTAGCGCTCGAG
TGTACGAGAGTCAAGATGATGCGGTGGCGGGCATTTTGGGTGGTGAAGTGGTGGCTGGCGATGTGGTGGTTATTCGCTAC
GAAGGCCCTAAAGGTGGCCCAGGCATGCAAGAGATGCTTTACCCAACGAGTTACTTGAAATCACGTGGCTTAGGTACTCA
ATGCGCATTGATCACCGATGGGCGCTTCTCGGGTGGTACTTCAGGCTTGTCGATAGGTCATGTTTCTCCAGAAGCTGCTG
CGGGTGGCACTATCGGTCTAGTGCAAACGGGCGATCGTATCGAAATTGATATCCCTGCACGTTCAATCAAGCTAGCAATC
AGTGATGTTGAGTTAGCGGCGCGTCGCACTGCAATGGAAGCACTTGGTAATGATGCGTGGAAGCCCCTTGGGCGAGTAAG
ACACGTATCTATGGCACTTAAAGCCTACGCATTATTAGCGACCAGTGCCGATAAGGGCGCGGTGCGTGATACTAGCAAGC
TAGTTTAA

Upstream 100 bases:

>100_bases
CGATATCTCAGAACATAGAATTAAAATTGAAGGTTGTGACAGGTTTTTAGGACCTAGGTCCTAAAAATCCAAGAACCTTC
TTTTAAACAAGGATAATGCA

Downstream 100 bases:

>100_bases
TCGATTTAACGGGATTAAGAGGTAGTTTATGTTGGCTCTAGCATCACAATCGCAACTGGATTTGGCTCACTATTATCTGC
AGAAAACTTTGCTGTCATCG

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 615; Mature: 614

Protein sequence:

>615_residues
MPKLRSATSTEGRNMAGARALWRATGVKDNDFGKPIIAISNSFTQFVPGHVHLKDMGSLVAGAIEEAGGIAKEFNTIAVD
DGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADALVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDKLI
KLDLVDAMVAGADERVSDADSEQIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSMLATHADRRELFLEAGRRIM
DLATRYYKHDDESALPRNIANFKAFENAMTLDIAMGGSSNTVLHLLAAAQEAEVDFTMDDIDRLSRLVPHLCKVAPATPK
YHMEDVHRAGGVMGILGELDRANLLHNDAYHVAGENLAAVLAKYDIAQSDDAAVRKFFSAGPAGIPTTKAFSQDCRWDSV
DDDRQQGCIRSREFAFSQEGGLAVLSGNVAVDGCIVKTAGVEVENHTFIGSARVYESQDDAVAGILGGEVVAGDVVVIRY
EGPKGGPGMQEMLYPTSYLKSRGLGTQCALITDGRFSGGTSGLSIGHVSPEAAAGGTIGLVQTGDRIEIDIPARSIKLAI
SDVELAARRTAMEALGNDAWKPLGRVRHVSMALKAYALLATSADKGAVRDTSKLV

Sequences:

>Translated_615_residues
MPKLRSATSTEGRNMAGARALWRATGVKDNDFGKPIIAISNSFTQFVPGHVHLKDMGSLVAGAIEEAGGIAKEFNTIAVD
DGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADALVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDKLI
KLDLVDAMVAGADERVSDADSEQIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSMLATHADRRELFLEAGRRIM
DLATRYYKHDDESALPRNIANFKAFENAMTLDIAMGGSSNTVLHLLAAAQEAEVDFTMDDIDRLSRLVPHLCKVAPATPK
YHMEDVHRAGGVMGILGELDRANLLHNDAYHVAGENLAAVLAKYDIAQSDDAAVRKFFSAGPAGIPTTKAFSQDCRWDSV
DDDRQQGCIRSREFAFSQEGGLAVLSGNVAVDGCIVKTAGVEVENHTFIGSARVYESQDDAVAGILGGEVVAGDVVVIRY
EGPKGGPGMQEMLYPTSYLKSRGLGTQCALITDGRFSGGTSGLSIGHVSPEAAAGGTIGLVQTGDRIEIDIPARSIKLAI
SDVELAARRTAMEALGNDAWKPLGRVRHVSMALKAYALLATSADKGAVRDTSKLV
>Mature_614_residues
PKLRSATSTEGRNMAGARALWRATGVKDNDFGKPIIAISNSFTQFVPGHVHLKDMGSLVAGAIEEAGGIAKEFNTIAVDD
GIAMGHGGMLYSLPSRELIADSVEYMVNAHCADALVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDKLIK
LDLVDAMVAGADERVSDADSEQIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSMLATHADRRELFLEAGRRIMD
LATRYYKHDDESALPRNIANFKAFENAMTLDIAMGGSSNTVLHLLAAAQEAEVDFTMDDIDRLSRLVPHLCKVAPATPKY
HMEDVHRAGGVMGILGELDRANLLHNDAYHVAGENLAAVLAKYDIAQSDDAAVRKFFSAGPAGIPTTKAFSQDCRWDSVD
DDRQQGCIRSREFAFSQEGGLAVLSGNVAVDGCIVKTAGVEVENHTFIGSARVYESQDDAVAGILGGEVVAGDVVVIRYE
GPKGGPGMQEMLYPTSYLKSRGLGTQCALITDGRFSGGTSGLSIGHVSPEAAAGGTIGLVQTGDRIEIDIPARSIKLAIS
DVELAARRTAMEALGNDAWKPLGRVRHVSMALKAYALLATSADKGAVRDTSKLV

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=614, Percent_Identity=78.1758957654723, Blast_Score=995, Evalue=0.0,
Organism=Escherichia coli, GI1788157, Length=537, Percent_Identity=30.9124767225326, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI2367371, Length=490, Percent_Identity=31.0204081632653, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI1786464, Length=522, Percent_Identity=30.8429118773946, Blast_Score=165, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6322476, Length=609, Percent_Identity=37.6026272577997, Blast_Score=335, Evalue=9e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 65100; Mature: 64969

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLRSATSTEGRNMAGARALWRATGVKDNDFGKPIIAISNSFTQFVPGHVHLKDMGSLV
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHCCCEEEHHHHHHHH
AGAIEEAGGIAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADALVCIS
HHHHHHHCCHHHHCCEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDKLIKLDLVDAMVAGADERVSDAD
CCCCCCHHHHHHEEECCCEEEEEECCCCCCCCHHHHHHHEEHHHHHHHHHCCCCCCCCCC
SEQIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSMLATHADRRELFLEAGRRIM
HHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHCHHHH
DLATRYYKHDDESALPRNIANFKAFENAMTLDIAMGGSSNTVLHLLAAAQEAEVDFTMDD
HHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCHHH
IDRLSRLVPHLCKVAPATPKYHMEDVHRAGGVMGILGELDRANLLHNDAYHVAGENLAAV
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEHHHCCHHHHHHCCCCHHCCCCHHHHH
LAKYDIAQSDDAAVRKFFSAGPAGIPTTKAFSQDCRWDSVDDDRQQGCIRSREFAFSQEG
HHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCC
GLAVLSGNVAVDGCIVKTAGVEVENHTFIGSARVYESQDDAVAGILGGEVVAGDVVVIRY
CEEEEECCEEEEEEEEEECCCEEECCEEEECEEEECCCCCCEEEEECCCEEECCEEEEEE
EGPKGGPGMQEMLYPTSYLKSRGLGTQCALITDGRFSGGTSGLSIGHVSPEAAAGGTIGL
CCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCCEEE
VQTGDRIEIDIPARSIKLAISDVELAARRTAMEALGNDAWKPLGRVRHVSMALKAYALLA
EECCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHE
TSADKGAVRDTSKLV
ECCCCCCCCHHHCCC
>Mature Secondary Structure 
PKLRSATSTEGRNMAGARALWRATGVKDNDFGKPIIAISNSFTQFVPGHVHLKDMGSLV
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHCCCEEEHHHHHHHH
AGAIEEAGGIAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADALVCIS
HHHHHHHCCHHHHCCEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDKLIKLDLVDAMVAGADERVSDAD
CCCCCCHHHHHHEEECCCEEEEEECCCCCCCCHHHHHHHEEHHHHHHHHHCCCCCCCCCC
SEQIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSMLATHADRRELFLEAGRRIM
HHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHCHHHH
DLATRYYKHDDESALPRNIANFKAFENAMTLDIAMGGSSNTVLHLLAAAQEAEVDFTMDD
HHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCHHH
IDRLSRLVPHLCKVAPATPKYHMEDVHRAGGVMGILGELDRANLLHNDAYHVAGENLAAV
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEHHHCCHHHHHHCCCCHHCCCCHHHHH
LAKYDIAQSDDAAVRKFFSAGPAGIPTTKAFSQDCRWDSVDDDRQQGCIRSREFAFSQEG
HHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCC
GLAVLSGNVAVDGCIVKTAGVEVENHTFIGSARVYESQDDAVAGILGGEVVAGDVVVIRY
CEEEEECCEEEEEEEEEECCCEEECCEEEECEEEECCCCCCEEEEECCCEEECCEEEEEE
EGPKGGPGMQEMLYPTSYLKSRGLGTQCALITDGRFSGGTSGLSIGHVSPEAAAGGTIGL
CCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCCEEE
VQTGDRIEIDIPARSIKLAISDVELAARRTAMEALGNDAWKPLGRVRHVSMALKAYALLA
EECCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHE
TSADKGAVRDTSKLV
ECCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA