Click here to switch to the map view.

The map label for this gene is ilvE [H]

Identifier: 157960176

GI number: 157960176

Start: 393352

End: 394281

Strand: Direct

Name: ilvE [H]

Synonym: Spea_0347

Alternate gene names: 157960176

Gene position: 393352-394281 (Clockwise)

Preceding gene: 157960175

Following gene: 157960177

Centisome position: 7.6

GC content: 49.46

Gene sequence:

>930_bases
ATGGCCGCTAAAACAGCAGAGTTAATTTGGTTCAATGGTGCAATGATGCCTTGGGGTGATGCTAAGGTTCATGTCATGTC
TCACGCGCTGCACTATGGCACGTCAGTATTTGAAGGCATTCGTGTTTACGATACCCACAGCGGTCCTGCCGGGTTTCGCT
TAACTGAGCACGTTCAGCGCCTGTTTGATTCGGCCAAGATTTATCGTATGCCAATTCCTTATAGCTTCGATGAGGTGATG
CAGGCCTGTCGTGATGCCACTCTCGGTAATAGCTTAAAAAGTGCCTACATCCGCCCCTTAGCTTTTTATGGTGATGTGGG
TATGGGGATCACCCCGCCAAAAGATGCTCAGTGTGACTTGCTGGTTGCAGCATTCCCTTGGGGAGCTTATCTAGGGGAAG
ACAGCATGGATGCCGGAGTGGATGTGGCAGTGACCTCATGGAATCGCTTAGCGCCGAACACTATCCCTACTGGCGCTAAA
GCGGGCGGTAATTACCTGTCTTCACTGCAGATCTCTACCGAAGCTAAGCGTAACGGCTTCGATGAAGGTATCGCGCTGGA
CACTAACGGTTTAGTCAGCGAAGCAGCCGGTGCAAATATTTTTGTCATTAAAAAGAACAAGATCTACACACCACCTGCTA
CAGCCGCTATTCTGATGGGGCTAACTCGAGACAGCATTATCACCCTTGCACGAGACAAAGGTTATCAAGTAGTTGAAGAG
CCAATGTCACGTGAATTTCTCTACCTTGCCGATGAGATCTTTATGACGGGCACCGCTGCTGAGATTGTGCCAGTGCGCAG
TGTTGATCGCATCGAAGTCGGCAGTGGCAAGCGCGGTGAGATCACTAAAGAAATTCAACAGAGTTTCTTCGGCCTGTTTA
CTGGTGAAACCGAGGATAAATGGGGTTGGTTGGAGACTTTGGAAAGCTAA

Upstream 100 bases:

>100_bases
TTGCTAACTAATCAAATCAATAAGCTTATCGATGTGATTGACTGCCGAGTGTTGTTAAGCAGTCCCACAGCAGAATTAGT
ACAACAAAAAGGGTGAGAAA

Downstream 100 bases:

>100_bases
AGCAGGTGCAAGGTCCTAGGTTCTAGGAAAAGCTAAGAAAGGTCCTAGGAAAGCACAAGCAGAGACGAACGCTTCGCTGA
CGGCAAGGCCGGAAAAGATT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MAAKTAELIWFNGAMMPWGDAKVHVMSHALHYGTSVFEGIRVYDTHSGPAGFRLTEHVQRLFDSAKIYRMPIPYSFDEVM
QACRDATLGNSLKSAYIRPLAFYGDVGMGITPPKDAQCDLLVAAFPWGAYLGEDSMDAGVDVAVTSWNRLAPNTIPTGAK
AGGNYLSSLQISTEAKRNGFDEGIALDTNGLVSEAAGANIFVIKKNKIYTPPATAAILMGLTRDSIITLARDKGYQVVEE
PMSREFLYLADEIFMTGTAAEIVPVRSVDRIEVGSGKRGEITKEIQQSFFGLFTGETEDKWGWLETLES

Sequences:

>Translated_309_residues
MAAKTAELIWFNGAMMPWGDAKVHVMSHALHYGTSVFEGIRVYDTHSGPAGFRLTEHVQRLFDSAKIYRMPIPYSFDEVM
QACRDATLGNSLKSAYIRPLAFYGDVGMGITPPKDAQCDLLVAAFPWGAYLGEDSMDAGVDVAVTSWNRLAPNTIPTGAK
AGGNYLSSLQISTEAKRNGFDEGIALDTNGLVSEAAGANIFVIKKNKIYTPPATAAILMGLTRDSIITLARDKGYQVVEE
PMSREFLYLADEIFMTGTAAEIVPVRSVDRIEVGSGKRGEITKEIQQSFFGLFTGETEDKWGWLETLES
>Mature_308_residues
AAKTAELIWFNGAMMPWGDAKVHVMSHALHYGTSVFEGIRVYDTHSGPAGFRLTEHVQRLFDSAKIYRMPIPYSFDEVMQ
ACRDATLGNSLKSAYIRPLAFYGDVGMGITPPKDAQCDLLVAAFPWGAYLGEDSMDAGVDVAVTSWNRLAPNTIPTGAKA
GGNYLSSLQISTEAKRNGFDEGIALDTNGLVSEAAGANIFVIKKNKIYTPPATAAILMGLTRDSIITLARDKGYQVVEEP
MSREFLYLADEIFMTGTAAEIVPVRSVDRIEVGSGKRGEITKEIQQSFFGLFTGETEDKWGWLETLES

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=282, Percent_Identity=26.5957446808511, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI38176287, Length=329, Percent_Identity=24.9240121580547, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI296010906, Length=329, Percent_Identity=24.9240121580547, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI296010904, Length=329, Percent_Identity=24.9240121580547, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI48994963, Length=307, Percent_Identity=64.4951140065147, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1787338, Length=253, Percent_Identity=26.0869565217391, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17568601, Length=285, Percent_Identity=27.719298245614, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17565728, Length=285, Percent_Identity=25.2631578947368, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322002, Length=318, Percent_Identity=26.1006289308176, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322608, Length=336, Percent_Identity=25.297619047619, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24641779, Length=281, Percent_Identity=27.0462633451957, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 33772; Mature: 33641

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKTAELIWFNGAMMPWGDAKVHVMSHALHYGTSVFEGIRVYDTHSGPAGFRLTEHVQR
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHH
LFDSAKIYRMPIPYSFDEVMQACRDATLGNSLKSAYIRPLAFYGDVGMGITPPKDAQCDL
HHCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHCEEEEECCCCCCCCCCCCCCCCE
LVAAFPWGAYLGEDSMDAGVDVAVTSWNRLAPNTIPTGAKAGGNYLSSLQISTEAKRNGF
EEEECCCCHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHEEEEECHHHHCCC
DEGIALDTNGLVSEAAGANIFVIKKNKIYTPPATAAILMGLTRDSIITLARDKGYQVVEE
CCCEEECCCCCHHHCCCCEEEEEECCEEECCCHHHHHHHHCCCCCEEEEECCCCCHHHHH
PMSREFLYLADEIFMTGTAAEIVPVRSVDRIEVGSGKRGEITKEIQQSFFGLFTGETEDK
HHCCHHHEEEHHHHCCCCCCEEEECCCCCEEECCCCCCCCHHHHHHHHHHHEECCCCCCC
WGWLETLES
CHHHHHHCC
>Mature Secondary Structure 
AAKTAELIWFNGAMMPWGDAKVHVMSHALHYGTSVFEGIRVYDTHSGPAGFRLTEHVQR
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHH
LFDSAKIYRMPIPYSFDEVMQACRDATLGNSLKSAYIRPLAFYGDVGMGITPPKDAQCDL
HHCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHCEEEEECCCCCCCCCCCCCCCCE
LVAAFPWGAYLGEDSMDAGVDVAVTSWNRLAPNTIPTGAKAGGNYLSSLQISTEAKRNGF
EEEECCCCHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHEEEEECHHHHCCC
DEGIALDTNGLVSEAAGANIFVIKKNKIYTPPATAAILMGLTRDSIITLARDKGYQVVEE
CCCEEECCCCCHHHCCCCEEEEEECCEEECCCHHHHHHHHCCCCCEEEEECCCCCHHHHH
PMSREFLYLADEIFMTGTAAEIVPVRSVDRIEVGSGKRGEITKEIQQSFFGLFTGETEDK
HHCCHHHEEEHHHHCCCCCCEEEECCCCCEEECCCCCCCCHHHHHHHHHHHEECCCCCCC
WGWLETLES
CHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]