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The map label for this gene is yafC [C]

Identifier: 157960095

GI number: 157960095

Start: 299132

End: 300001

Strand: Reverse

Name: yafC [C]

Synonym: Spea_0266

Alternate gene names: 157960095

Gene position: 300001-299132 (Counterclockwise)

Preceding gene: 157960101

Following gene: 157960093

Centisome position: 5.8

GC content: 47.7

Gene sequence:

>870_bases
ATGTTTAATTGGGAAGGTGTGAGTGAGTTCGTTGCCGTCGCAGAAGCCGAAAGCTTTACCAAGGCATCGCAACGGTTAGG
GATCTCGACTGCGCAGGTTAGTCGGCAGATAAGTGCATTAGAAACACGCTTAGCCTCTAAACTGTTTCATCGCACCACCA
GAAAGGTATCTGTTACCGAAGTGGGTCGGGTCTATTATCAGCATTGTAGACAGGTACTCGATGGCCTCGAAGAAGCCGAG
CGCGCCATTACTAACTTACAAACTACCCCAAGAGGCCTGTTAAAGATCACTGCGCCTGTAACCTATGGTGAAAAAACCAT
TGCTCCACTAATTAATGACTTTGCATCGCTGTATCCTGAGCTTGAAGTCAAACTCAATCTAAGCAATCAGAAGTTAGACC
TAGTCGATGAGGGCTATGATCTGGCGATCCGCCTTGGCCATTTAGAAGATTCAAGCATGATGGCTAAGAAGCTCAGCAGC
CGAACTCAATATGTGTGTGCCTCGCCAAACTACTTAGCAACTTATGGTTTACCCCATTCACTATCTGAACTTGATCAGCA
TAACTGTTTAATTGGCACCATGGACTATTGGCGCTTTCAAGAGAGCGGTAAAACCAGGAATGTACGGGTTAAAGGCAGCT
TGAGTTGCAACAGTGGCCATGCCCTGGTTGATGCAGCCATTAAAGGCATAGGCATAGTGCAGCTGCCCGATTATTACGTA
TTACCCTTTATTGAATCGGGTGAGCTAACCCCCTTGCTCGAGCAAAACCGCCAACCCGAAGAAGGGATCTGGGCGCTGTA
CCCACAAAATCGCCACCTTTCGCCTAAGGTACGCATGCTGCTGGATTTCCTGAGTGAAGGCCTAAAATAG

Upstream 100 bases:

>100_bases
AGATATTGGTTTTGTCGATGATTAAATTAAAAATTGCTTTTACCTCATTTATGTTAATAATTAAGCCATATTTTCCAGCC
ATAATTTCTAGAGAGTAACA

Downstream 100 bases:

>100_bases
AGACTAGGCCCCACTCGCTTTGGCCTGCTGAGTGATATGCTTATCACTCAGCAGCAAAGTAGACCTGATTACGACCACGC
TGTTTTGCTTGATATAACGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MFNWEGVSEFVAVAEAESFTKASQRLGISTAQVSRQISALETRLASKLFHRTTRKVSVTEVGRVYYQHCRQVLDGLEEAE
RAITNLQTTPRGLLKITAPVTYGEKTIAPLINDFASLYPELEVKLNLSNQKLDLVDEGYDLAIRLGHLEDSSMMAKKLSS
RTQYVCASPNYLATYGLPHSLSELDQHNCLIGTMDYWRFQESGKTRNVRVKGSLSCNSGHALVDAAIKGIGIVQLPDYYV
LPFIESGELTPLLEQNRQPEEGIWALYPQNRHLSPKVRMLLDFLSEGLK

Sequences:

>Translated_289_residues
MFNWEGVSEFVAVAEAESFTKASQRLGISTAQVSRQISALETRLASKLFHRTTRKVSVTEVGRVYYQHCRQVLDGLEEAE
RAITNLQTTPRGLLKITAPVTYGEKTIAPLINDFASLYPELEVKLNLSNQKLDLVDEGYDLAIRLGHLEDSSMMAKKLSS
RTQYVCASPNYLATYGLPHSLSELDQHNCLIGTMDYWRFQESGKTRNVRVKGSLSCNSGHALVDAAIKGIGIVQLPDYYV
LPFIESGELTPLLEQNRQPEEGIWALYPQNRHLSPKVRMLLDFLSEGLK
>Mature_289_residues
MFNWEGVSEFVAVAEAESFTKASQRLGISTAQVSRQISALETRLASKLFHRTTRKVSVTEVGRVYYQHCRQVLDGLEEAE
RAITNLQTTPRGLLKITAPVTYGEKTIAPLINDFASLYPELEVKLNLSNQKLDLVDEGYDLAIRLGHLEDSSMMAKKLSS
RTQYVCASPNYLATYGLPHSLSELDQHNCLIGTMDYWRFQESGKTRNVRVKGSLSCNSGHALVDAAIKGIGIVQLPDYYV
LPFIESGELTPLLEQNRQPEEGIWALYPQNRHLSPKVRMLLDFLSEGLK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=36.2068965517241, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1789440, Length=281, Percent_Identity=33.8078291814947, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI87081978, Length=282, Percent_Identity=31.5602836879433, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI145693193, Length=286, Percent_Identity=31.1188811188811, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1789639, Length=284, Percent_Identity=31.3380281690141, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=29.553264604811, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1787589, Length=283, Percent_Identity=31.095406360424, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790011, Length=284, Percent_Identity=26.056338028169, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI157672245, Length=128, Percent_Identity=35.15625, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789173, Length=243, Percent_Identity=25.1028806584362, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788297, Length=246, Percent_Identity=24.7967479674797, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788706, Length=250, Percent_Identity=25.6, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786448, Length=266, Percent_Identity=22.1804511278195, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693105, Length=165, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788481, Length=289, Percent_Identity=23.1833910034602, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787879, Length=286, Percent_Identity=23.7762237762238, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787601, Length=143, Percent_Identity=27.972027972028, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786713, Length=264, Percent_Identity=26.1363636363636, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32423; Mature: 32423

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNWEGVSEFVAVAEAESFTKASQRLGISTAQVSRQISALETRLASKLFHRTTRKVSVTE
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEHHHH
VGRVYYQHCRQVLDGLEEAERAITNLQTTPRGLLKITAPVTYGEKTIAPLINDFASLYPE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCC
LEVKLNLSNQKLDLVDEGYDLAIRLGHLEDSSMMAKKLSSRTQYVCASPNYLATYGLPHS
EEEEEECCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHCCCEEEECCCCEEEECCCCCC
LSELDQHNCLIGTMDYWRFQESGKTRNVRVKGSLSCNSGHALVDAAIKGIGIVQLPDYYV
HHHHHCCCCEEECHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHCCEEEECCCEEE
LPFIESGELTPLLEQNRQPEEGIWALYPQNRHLSPKVRMLLDFLSEGLK
EEEECCCCCCHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFNWEGVSEFVAVAEAESFTKASQRLGISTAQVSRQISALETRLASKLFHRTTRKVSVTE
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEHHHH
VGRVYYQHCRQVLDGLEEAERAITNLQTTPRGLLKITAPVTYGEKTIAPLINDFASLYPE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCC
LEVKLNLSNQKLDLVDEGYDLAIRLGHLEDSSMMAKKLSSRTQYVCASPNYLATYGLPHS
EEEEEECCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHCCCEEEECCCCEEEECCCCCC
LSELDQHNCLIGTMDYWRFQESGKTRNVRVKGSLSCNSGHALVDAAIKGIGIVQLPDYYV
HHHHHCCCCEEECHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHCCEEEECCCEEE
LPFIESGELTPLLEQNRQPEEGIWALYPQNRHLSPKVRMLLDFLSEGLK
EEEECCCCCCHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]