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The map label for this gene is proW [H]

Identifier: 157960085

GI number: 157960085

Start: 286571

End: 287470

Strand: Reverse

Name: proW [H]

Synonym: Spea_0256

Alternate gene names: 157960085

Gene position: 287470-286571 (Counterclockwise)

Preceding gene: 157960086

Following gene: 157960084

Centisome position: 5.56

GC content: 47.33

Gene sequence:

>900_bases
ATGGCTGACTTTTCTTGGCTCAGCGAGTTCCCAAAAATGGATCGCTCAACTTTATTAGCGATCCGTAAATATTTAGATGG
TGCCTTTCGCGACTTTTCCCGCGAATATGGTGATGTCATCGAATCTGCCTTCGATCCTCTACTCGACTTTCTTATCTGGT
TTGAAAAGCTACTAGTGCAATCACCCTGGTGGGCTGTACTGATCTCGATTACAGCTTTGGTTTACTTAGCTAGTCGCTCA
TGGAAATTATCTTTAGCCGTTGTGGTGTCATTTCTGCTGATTGGCTATTTTGGTATGTGGGAAGATACCATGCGCACCCT
TAGCATCATCACAGTCTGCACCCTATTAGCCATACTACTGGGCATCCCCATTGGCATCTTAATGTCTCGCAGCGATCGCA
TCCAGTCGATAGTCACACCGATTCTGGATGTGATGCAAACTATGCCAGCCTTTGTATACCTCATCCCTGTGGTCATGTTA
CTGGGAATAGGCAAGGTACCCGGCGTTATTGCGGTGGTCATTTATGCCATTCCACCTGTCATTCGTCTAACCAACTTAGG
CATTCGTCTGGTTGATAAGGAAGTGTTGGAGGCTGCCACTGCCTACGGTGCGAATCCGATGCAACGCTTGATGGGGGTTC
AATTGCCATTGGCCTCACCCACCATTATGGCTGGTATTAACCAGACGATTATGATGGCTCTAGGCATGGTGGTTATCGCA
TCGATGATCGGCGTTAAGGGCTTAGGCCAACCTGTACTCAAGTCGATCACCAACCAATATTTCACTCTCGGATTATTTAA
TGGTTTAGCCATCGTTGCCTTGGCTATTATTTTTGATCGCGCCTCCCAAGCCTACGCCAAGCGTTCGCAGAAACATATAC
AAGGCGGGCAACATGACTAG

Upstream 100 bases:

>100_bases
TATCGCAAACCTATAACGCTAAATCGCTACGCGAGGCCTACTTCGCCTCGTGATCTGTCTTTTCACCTAGAAAATCCCTT
AAAAAACTGGAGAGGTAGCA

Downstream 100 bases:

>100_bases
CGACTCGCAAACACAACCACTGATTCAAATTAAGGATCTGTTTAAGGTCTTTGGCAAAAAACCAGAGCAGGTCATGCCAA
TGGTTAGGGATGGTCTTTCT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MADFSWLSEFPKMDRSTLLAIRKYLDGAFRDFSREYGDVIESAFDPLLDFLIWFEKLLVQSPWWAVLISITALVYLASRS
WKLSLAVVVSFLLIGYFGMWEDTMRTLSIITVCTLLAILLGIPIGILMSRSDRIQSIVTPILDVMQTMPAFVYLIPVVML
LGIGKVPGVIAVVIYAIPPVIRLTNLGIRLVDKEVLEAATAYGANPMQRLMGVQLPLASPTIMAGINQTIMMALGMVVIA
SMIGVKGLGQPVLKSITNQYFTLGLFNGLAIVALAIIFDRASQAYAKRSQKHIQGGQHD

Sequences:

>Translated_299_residues
MADFSWLSEFPKMDRSTLLAIRKYLDGAFRDFSREYGDVIESAFDPLLDFLIWFEKLLVQSPWWAVLISITALVYLASRS
WKLSLAVVVSFLLIGYFGMWEDTMRTLSIITVCTLLAILLGIPIGILMSRSDRIQSIVTPILDVMQTMPAFVYLIPVVML
LGIGKVPGVIAVVIYAIPPVIRLTNLGIRLVDKEVLEAATAYGANPMQRLMGVQLPLASPTIMAGINQTIMMALGMVVIA
SMIGVKGLGQPVLKSITNQYFTLGLFNGLAIVALAIIFDRASQAYAKRSQKHIQGGQHD
>Mature_298_residues
ADFSWLSEFPKMDRSTLLAIRKYLDGAFRDFSREYGDVIESAFDPLLDFLIWFEKLLVQSPWWAVLISITALVYLASRSW
KLSLAVVVSFLLIGYFGMWEDTMRTLSIITVCTLLAILLGIPIGILMSRSDRIQSIVTPILDVMQTMPAFVYLIPVVMLL
GIGKVPGVIAVVIYAIPPVIRLTNLGIRLVDKEVLEAATAYGANPMQRLMGVQLPLASPTIMAGINQTIMMALGMVVIAS
MIGVKGLGQPVLKSITNQYFTLGLFNGLAIVALAIIFDRASQAYAKRSQKHIQGGQHD

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=295, Percent_Identity=42.7118644067797, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1788449, Length=189, Percent_Identity=31.2169312169312, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788451, Length=191, Percent_Identity=31.413612565445, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786564, Length=152, Percent_Identity=29.6052631578947, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32971; Mature: 32840

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADFSWLSEFPKMDRSTLLAIRKYLDGAFRDFSREYGDVIESAFDPLLDFLIWFEKLLVQ
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SPWWAVLISITALVYLASRSWKLSLAVVVSFLLIGYFGMWEDTMRTLSIITVCTLLAILL
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIPIGILMSRSDRIQSIVTPILDVMQTMPAFVYLIPVVMLLGIGKVPGVIAVVIYAIPPV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IRLTNLGIRLVDKEVLEAATAYGANPMQRLMGVQLPLASPTIMAGINQTIMMALGMVVIA
HHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SMIGVKGLGQPVLKSITNQYFTLGLFNGLAIVALAIIFDRASQAYAKRSQKHIQGGQHD
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ADFSWLSEFPKMDRSTLLAIRKYLDGAFRDFSREYGDVIESAFDPLLDFLIWFEKLLVQ
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SPWWAVLISITALVYLASRSWKLSLAVVVSFLLIGYFGMWEDTMRTLSIITVCTLLAILL
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIPIGILMSRSDRIQSIVTPILDVMQTMPAFVYLIPVVMLLGIGKVPGVIAVVIYAIPPV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IRLTNLGIRLVDKEVLEAATAYGANPMQRLMGVQLPLASPTIMAGINQTIMMALGMVVIA
HHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SMIGVKGLGQPVLKSITNQYFTLGLFNGLAIVALAIIFDRASQAYAKRSQKHIQGGQHD
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]