Click here to switch to the map view.

The map label for this gene is argF [H]

Identifier: 157960058

GI number: 157960058

Start: 257598

End: 258503

Strand: Direct

Name: argF [H]

Synonym: Spea_0229

Alternate gene names: 157960058

Gene position: 257598-258503 (Clockwise)

Preceding gene: 157960057

Following gene: 157960059

Centisome position: 4.98

GC content: 45.58

Gene sequence:

>906_bases
ATGAAACACCTACTATCAATTAAAGATCTCAGCCAGCAGCAATTACTCGACCTATTGGCGTTGGCACAAAACATTAAGGC
TAATCCTGCCGAATATAAGCATGCCTTGGATGGCAAGAGTGTGGTGATGTTATTTGAGAAGCCATCTCTGAGAACTCGGG
TGAGTTTTGATATTGGCATTAATAAGCTTGGTGGCCACTGCTTATATCTAGATCAACAAAATGGCGCCCTAGGTAAGCGT
GAGCCGGTGTCAGACTTTGCTGCCAATATTTCTTGTTGGGCTGATGCCATTGTCGCCCGTACATTTTTACACTCGACAAT
AGAAGAGCTAGCAGAGCATGGCACAGTACCGGTTATTAATGCGCTATCGGATCTTTATCACCCTTGCCAAGGTCTAGCGG
ATTTTCTGACATTAACCGAGCAATATGGCGATGTGAGCAAGGTGAAATTGGCTTATGTTGGTGATGGCAACAACGTAACT
CATTCATTGATGTTTGGCGCAGCTATCTTAGGTGCACAGTTAACCGTCATCTGTCCTCCTGGCCACTTCCCTGATGGTAA
GGTTGTTTGTGAAGCGCAGGAGTTAGCGGCAAAACATGGCGGTAAGTTAACTCTTAGTTCAGATATCGAAGCGATTGGCG
ACCATCATGCTATTTACACCGACACCTGGATATCTATGGGCGACTCAGCATCTATGGAAGAGATCGAGGCCAAGTTTAAG
CCCTATCAAGTGAATGCCGAGTTGATGCAAAAGGCGGGGGCGAAACATTTCATGCATTGCTTACCAGCTTATCGAGAAGT
TGAAGTGACTGCCGAGATAGTTGATGGAGACGGCTCACTCATCTTACATCAGGCCGAAAATAGAATGCATGCTCAAAATG
CCGTGCTGGTAACGCTTTTAAGTTAA

Upstream 100 bases:

>100_bases
AGCCGTACAGATCGCCTCTTGGCGTCATGCACATCAGTTAATCGCATTATGCCAGGGTGAAGCCGTTGGCACACAGATCC
AACCTTAGGAGATAAGTCTT

Downstream 100 bases:

>100_bases
TTACTTTTTGAAAAATAACTATCAATCTGCATTGGCAGATCGACATAAAAAACAGTTAGTAGGAAATTAAGATGTCTATT
GAAAATTCAGCAGTAAGTGA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKHLLSIKDLSQQQLLDLLALAQNIKANPAEYKHALDGKSVVMLFEKPSLRTRVSFDIGINKLGGHCLYLDQQNGALGKR
EPVSDFAANISCWADAIVARTFLHSTIEELAEHGTVPVINALSDLYHPCQGLADFLTLTEQYGDVSKVKLAYVGDGNNVT
HSLMFGAAILGAQLTVICPPGHFPDGKVVCEAQELAAKHGGKLTLSSDIEAIGDHHAIYTDTWISMGDSASMEEIEAKFK
PYQVNAELMQKAGAKHFMHCLPAYREVEVTAEIVDGDGSLILHQAENRMHAQNAVLVTLLS

Sequences:

>Translated_301_residues
MKHLLSIKDLSQQQLLDLLALAQNIKANPAEYKHALDGKSVVMLFEKPSLRTRVSFDIGINKLGGHCLYLDQQNGALGKR
EPVSDFAANISCWADAIVARTFLHSTIEELAEHGTVPVINALSDLYHPCQGLADFLTLTEQYGDVSKVKLAYVGDGNNVT
HSLMFGAAILGAQLTVICPPGHFPDGKVVCEAQELAAKHGGKLTLSSDIEAIGDHHAIYTDTWISMGDSASMEEIEAKFK
PYQVNAELMQKAGAKHFMHCLPAYREVEVTAEIVDGDGSLILHQAENRMHAQNAVLVTLLS
>Mature_301_residues
MKHLLSIKDLSQQQLLDLLALAQNIKANPAEYKHALDGKSVVMLFEKPSLRTRVSFDIGINKLGGHCLYLDQQNGALGKR
EPVSDFAANISCWADAIVARTFLHSTIEELAEHGTVPVINALSDLYHPCQGLADFLTLTEQYGDVSKVKLAYVGDGNNVT
HSLMFGAAILGAQLTVICPPGHFPDGKVVCEAQELAAKHGGKLTLSSDIEAIGDHHAIYTDTWISMGDSASMEEIEAKFK
PYQVNAELMQKAGAKHFMHCLPAYREVEVTAEIVDGDGSLILHQAENRMHAQNAVLVTLLS

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=305, Percent_Identity=39.672131147541, Blast_Score=214, Evalue=6e-56,
Organism=Homo sapiens, GI18105007, Length=308, Percent_Identity=29.2207792207792, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=34.8623853211009, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI1786469, Length=326, Percent_Identity=34.6625766871166, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI2367364, Length=310, Percent_Identity=29.3548387096774, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI48994908, Length=301, Percent_Identity=24.9169435215947, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=324, Percent_Identity=26.2345679012346, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322373, Length=310, Percent_Identity=32.258064516129, Blast_Score=176, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6322331, Length=193, Percent_Identity=29.5336787564767, Blast_Score=82, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24642586, Length=307, Percent_Identity=25.7328990228013, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 32741; Mature: 32741

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLLSIKDLSQQQLLDLLALAQNIKANPAEYKHALDGKSVVMLFEKPSLRTRVSFDIGI
CCCCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCCCCEEEEEECCC
NKLGGHCLYLDQQNGALGKREPVSDFAANISCWADAIVARTFLHSTIEELAEHGTVPVIN
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
ALSDLYHPCQGLADFLTLTEQYGDVSKVKLAYVGDGNNVTHSLMFGAAILGAQLTVICPP
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHCCEEEEEECC
GHFPDGKVVCEAQELAAKHGGKLTLSSDIEAIGDHHAIYTDTWISMGDSASMEEIEAKFK
CCCCCCCEEEEHHHHHHHCCCEEEECCCHHHHCCCCEEEEEHHHCCCCCCCHHHHHHHCC
PYQVNAELMQKAGAKHFMHCLPAYREVEVTAEIVDGDGSLILHQAENRMHAQNAVLVTLL
CEEECHHHHHHCCHHHHHHHHCCHHEEEEEEEEECCCCCEEEEECCCCHHHCCEEEEEEE
S
C
>Mature Secondary Structure
MKHLLSIKDLSQQQLLDLLALAQNIKANPAEYKHALDGKSVVMLFEKPSLRTRVSFDIGI
CCCCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCCCCEEEEEECCC
NKLGGHCLYLDQQNGALGKREPVSDFAANISCWADAIVARTFLHSTIEELAEHGTVPVIN
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
ALSDLYHPCQGLADFLTLTEQYGDVSKVKLAYVGDGNNVTHSLMFGAAILGAQLTVICPP
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHCCEEEEEECC
GHFPDGKVVCEAQELAAKHGGKLTLSSDIEAIGDHHAIYTDTWISMGDSASMEEIEAKFK
CCCCCCCEEEEHHHHHHHCCCEEEECCCHHHHCCCCEEEEEHHHCCCCCCCHHHHHHHCC
PYQVNAELMQKAGAKHFMHCLPAYREVEVTAEIVDGDGSLILHQAENRMHAQNAVLVTLL
CEEECHHHHHHCCHHHHHHHHCCHHEEEEEEEEECCCCCEEEEECCCCHHHCCEEEEEEE
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]