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The map label for this gene is argG [H]

Identifier: 157960059

GI number: 157960059

Start: 258575

End: 259813

Strand: Direct

Name: argG [H]

Synonym: Spea_0230

Alternate gene names: 157960059

Gene position: 258575-259813 (Clockwise)

Preceding gene: 157960058

Following gene: 157960060

Centisome position: 5.0

GC content: 48.35

Gene sequence:

>1239_bases
ATGTCTATTGAAAATTCAGCAGTAAGTGATCACTCAGGCGTTAACAAGGTTGTTCTGGCATATTCCGGTGGCTTAGACAC
CTCGGCGATTATTCCTTGGTTAAAAGAAACTTATGATAACTGTGAAATTGTGGCCTTTTGTGCCGATGTTGGCCAAGGTG
ATGCAGAGCTAGAAGGCTTGTATGAAAAAGCCATCGCCTCTGGCGCATCTGAATGCTACATCGTCGACTTAAAAGAGGAG
CTGGTGGCCGATTATATCTACCCAACGATTGCGACTGGTGCTATTTACGAAGGTACTTACCTGTTAGGTACGTCGATGGC
GAGGCCGATTATTGCTAAGGCACAAGTTGAAGTTGCTCGTAAAGTAGGCGCAGATGCGGTTTGTCACGGCTGTACTGGTA
AAGGCAACGATCAGGTTCGTTTCGAAGGCTGTTTTGCTGCATTAGCGCCTGATCTAAAAGTCATTGCGCCTTGGCGTGAG
TGGGAGATGGTGAGCCGTGAAGACCTGCTCGATTATCTTGCAGAGCGTAATATTGCTACTGCAGCGTCTGCGACCAAGAT
CTATAGCCGTGATGCAAACGCTTGGCATATTTCTCACGAAGGCGGCGAGCTAGAAGATCCATGGAATGAGCCATCGAAAG
GGGTTTGGACCATGACTGTCGCCCCAGAGGATGCGCCGAATCAGCCTGAATATGTGTCACTAGAGCTTGAGCAAGGCAAG
ATCACTAAGGTAAACGGTGAGGCTTTATCACCATATAAAGCCTTGATGGTATTGAATGAAGTAGCCGGTGCTCACGGTGT
TGGCAGAATCGATATTACCGAAAACAGACTGGTTGGCATGAAGTCTCGTGGTTGCTATGAAACGCCCGGTGGTACAGTGA
TGTTCGCAGCACTGCGCGCGATTGAAGAGTTAGTACTTGATAAAACCAGCCGCGAGTGGCGTGAGCAAGTTGGCGCGCAG
ATGGCACACCTTGTCTATGATGGTCGCTGGTTCACGCCATTGTGTGAGTCTCTGCTAGGTGCATCTAAGCCTTTAGCCGA
CTTAGTGAATGGTGAGGTAGTGGTCAAACTCTACAAGGGCCAGGCCAGCGTAGTTAAGAAGCGTTCTCCTAATAGTCTGT
ATTCTGAGGAGTTCGCGACCTTTGGTGCCGATGATGTTTATAACCAAAAAGATGCGGAAGGCTTCATTCGTTTGTATTCG
CTTTCAAGCCGGATCAGAGCGTTACATAGTCAAAAGTAA

Upstream 100 bases:

>100_bases
ATGCCGTGCTGGTAACGCTTTTAAGTTAATTACTTTTTGAAAAATAACTATCAATCTGCATTGGCAGATCGACATAAAAA
ACAGTTAGTAGGAAATTAAG

Downstream 100 bases:

>100_bases
GTCTTTGACCAATAAAAGTTAGTTAGCGCTAACGTGTATGAGTTAGCGCTCTATAAACATAGAGTTAAAGGTGAAGCTAG
ACTTTGCCTAGATATAAAGA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MSIENSAVSDHSGVNKVVLAYSGGLDTSAIIPWLKETYDNCEIVAFCADVGQGDAELEGLYEKAIASGASECYIVDLKEE
LVADYIYPTIATGAIYEGTYLLGTSMARPIIAKAQVEVARKVGADAVCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWRE
WEMVSREDLLDYLAERNIATAASATKIYSRDANAWHISHEGGELEDPWNEPSKGVWTMTVAPEDAPNQPEYVSLELEQGK
ITKVNGEALSPYKALMVLNEVAGAHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAIEELVLDKTSREWREQVGAQ
MAHLVYDGRWFTPLCESLLGASKPLADLVNGEVVVKLYKGQASVVKKRSPNSLYSEEFATFGADDVYNQKDAEGFIRLYS
LSSRIRALHSQK

Sequences:

>Translated_412_residues
MSIENSAVSDHSGVNKVVLAYSGGLDTSAIIPWLKETYDNCEIVAFCADVGQGDAELEGLYEKAIASGASECYIVDLKEE
LVADYIYPTIATGAIYEGTYLLGTSMARPIIAKAQVEVARKVGADAVCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWRE
WEMVSREDLLDYLAERNIATAASATKIYSRDANAWHISHEGGELEDPWNEPSKGVWTMTVAPEDAPNQPEYVSLELEQGK
ITKVNGEALSPYKALMVLNEVAGAHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAIEELVLDKTSREWREQVGAQ
MAHLVYDGRWFTPLCESLLGASKPLADLVNGEVVVKLYKGQASVVKKRSPNSLYSEEFATFGADDVYNQKDAEGFIRLYS
LSSRIRALHSQK
>Mature_411_residues
SIENSAVSDHSGVNKVVLAYSGGLDTSAIIPWLKETYDNCEIVAFCADVGQGDAELEGLYEKAIASGASECYIVDLKEEL
VADYIYPTIATGAIYEGTYLLGTSMARPIIAKAQVEVARKVGADAVCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREW
EMVSREDLLDYLAERNIATAASATKIYSRDANAWHISHEGGELEDPWNEPSKGVWTMTVAPEDAPNQPEYVSLELEQGKI
TKVNGEALSPYKALMVLNEVAGAHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAIEELVLDKTSREWREQVGAQM
AHLVYDGRWFTPLCESLLGASKPLADLVNGEVVVKLYKGQASVVKKRSPNSLYSEEFATFGADDVYNQKDAEGFIRLYSL
SSRIRALHSQK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=408, Percent_Identity=44.1176470588235, Blast_Score=321, Evalue=7e-88,
Organism=Homo sapiens, GI16950633, Length=408, Percent_Identity=44.1176470588235, Blast_Score=321, Evalue=7e-88,
Organism=Escherichia coli, GI1789563, Length=401, Percent_Identity=27.431421446384, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324514, Length=401, Percent_Identity=41.8952618453865, Blast_Score=305, Evalue=1e-83,
Organism=Drosophila melanogaster, GI21358151, Length=407, Percent_Identity=40.2948402948403, Blast_Score=289, Evalue=2e-78,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 44979; Mature: 44848

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIENSAVSDHSGVNKVVLAYSGGLDTSAIIPWLKETYDNCEIVAFCADVGQGDAELEGL
CCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHH
YEKAIASGASECYIVDLKEELVADYIYPTIATGAIYEGTYLLGTSMARPIIAKAQVEVAR
HHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCEECCEEEECCHHCCHHHHHHHHHHHH
KVGADAVCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWEMVSREDLLDYLAERNIAT
HCCCHHEEECCCCCCCCCEEEEEEHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCHHH
AASATKIYSRDANAWHISHEGGELEDPWNEPSKGVWTMTVAPEDAPNQPEYVSLELEQGK
HHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCE
ITKVNGEALSPYKALMVLNEVAGAHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRA
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCEECCCCCCCCCCCCCHHHHHHHHH
IEELVLDKTSREWREQVGAQMAHLVYDGRWFTPLCESLLGASKPLADLVNGEVVVKLYKG
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHCCCEEEEEEECC
QASVVKKRSPNSLYSEEFATFGADDVYNQKDAEGFIRLYSLSSRIRALHSQK
CHHHHHCCCCCCHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIENSAVSDHSGVNKVVLAYSGGLDTSAIIPWLKETYDNCEIVAFCADVGQGDAELEGL
CCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHH
YEKAIASGASECYIVDLKEELVADYIYPTIATGAIYEGTYLLGTSMARPIIAKAQVEVAR
HHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCEECCEEEECCHHCCHHHHHHHHHHHH
KVGADAVCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWEMVSREDLLDYLAERNIAT
HCCCHHEEECCCCCCCCCEEEEEEHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCHHH
AASATKIYSRDANAWHISHEGGELEDPWNEPSKGVWTMTVAPEDAPNQPEYVSLELEQGK
HHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCE
ITKVNGEALSPYKALMVLNEVAGAHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRA
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCEECCCCCCCCCCCCCHHHHHHHHH
IEELVLDKTSREWREQVGAQMAHLVYDGRWFTPLCESLLGASKPLADLVNGEVVVKLYKG
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHCCCEEEEEEECC
QASVVKKRSPNSLYSEEFATFGADDVYNQKDAEGFIRLYSLSSRIRALHSQK
CHHHHHCCCCCCHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA