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The map label for this gene is exeF [H]

Identifier: 157959976

GI number: 157959976

Start: 163491

End: 164714

Strand: Direct

Name: exeF [H]

Synonym: Spea_0146

Alternate gene names: 157959976

Gene position: 163491-164714 (Clockwise)

Preceding gene: 157959975

Following gene: 157959977

Centisome position: 3.16

GC content: 46.49

Gene sequence:

>1224_bases
ATGCCTGCATTTGAATACAAAGCATTAGACAAAACGGGTAAGCAGAAAAAAGGTGTTGTCGAAGCGGATACCGCAAGGCA
TGCACGTACTCAGCTAAGAGAGCAGCGCTTAATGCCACTGGAGATAACTCCAGTAGTAGAGAAAGAGAGCAAGACTAAGG
CCGCAGGTTTTAGCTTTTTAAAGCGTGGTATTTCAACTGCAGAGCTTGCACTTATTACCCGTCAGATTGCGACTCTAGTG
GCTGCTGGACTCCCTGTTGAAGAGGCATTAAAAGCCGTTGGTCAACAATGCGAGAAGGACCGTTTAGCCAGTATGGTGAT
GGCGGTGCGATCTCGGGTGGTTGAAGGTTATAGCCTTGCCGATTCTATGGCAGAGTTTCCACATATATTCGATGAATTGT
ATCGAGCTATGGTGGCATCGGGCGAAAAATCCGGTCACTTAGAAGTGGTACTGAATCGTCTCGCCGATTACACCGAGCGT
CGCCAGCAGCTAAAGAGTAAAATGACCCAAGCTATGATCTATCCTATCGTACTGACAGTTGTCGCCATAGGTGTGATCGC
AATTCTGCTTGCCGCCGTAGTGCCGCAAGTGGTGGGGCAGTTTGAGCATATGGGGCAGGAGTTACCTTGGACTACTGAAC
TATTGATCGCCTCATCAGACTTTATCCGTGATTATGGCCTAATTGTTCTGGCTGTTATCGTCGGTTTGTTCTTTATCGCC
AAACGTTTACTCGTTTCACCTAAAAATCGCATGAAATACGACAGCATGTTGCTGCGCCTACCTGTGATCAGCAAGGTGAG
TAAAAGCCTTAATACGGCACGTTTTGCTCGTACATTAAGTATTTTAACGGCCAGCTCTGTACCGCTACTCGATGCAATGC
GTATCGCGAGTGATGTACTGATCAACGTTAAAGTGAAAGCCGCAGTCGAAGATGCAACACTAAGGGTGCGTGAGGGCACA
AGTTTGGGAACGGCACTAGCCAACACTAAACTTTTCCCACCTATGATGCTCTATATGATTACCTCTGGTGAAAAAAGTGG
TCAACTTGAGCAGATGCTTGAGCGCGCCGCCGATAACCAAGACCGAGATTTTGAGTCGAATGTGACGATAGCCTTGGGGG
TATTTGAGCCTATGCTAGTTGTTAGTATGGCTGCTGTGGTTCTATTTATTGTTTTAGCGATTCTTCAGCCAATATTAGCG
CTGAATAATATGATTAGTGGTTAA

Upstream 100 bases:

>100_bases
TCAATCTGTTCCGAGCATTCGTCACGACGGTATGAGTAAAGTGCTTGCGGGGAAAACTACCCTAGAAGAAGTGCTGCGTG
TCACTCGAGAGGAGTAAGCC

Downstream 100 bases:

>100_bases
TACTCACAGTCTGCTACAGAGTCTTGTAGTTAGCGTGGGTGATATAGTTTAAGGAAGTTGTTAATGCAAGCACGTAATAA
ACAGAAAGGTTTCACCCTAT

Product: general secretion pathway protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MPAFEYKALDKTGKQKKGVVEADTARHARTQLREQRLMPLEITPVVEKESKTKAAGFSFLKRGISTAELALITRQIATLV
AAGLPVEEALKAVGQQCEKDRLASMVMAVRSRVVEGYSLADSMAEFPHIFDELYRAMVASGEKSGHLEVVLNRLADYTER
RQQLKSKMTQAMIYPIVLTVVAIGVIAILLAAVVPQVVGQFEHMGQELPWTTELLIASSDFIRDYGLIVLAVIVGLFFIA
KRLLVSPKNRMKYDSMLLRLPVISKVSKSLNTARFARTLSILTASSVPLLDAMRIASDVLINVKVKAAVEDATLRVREGT
SLGTALANTKLFPPMMLYMITSGEKSGQLEQMLERAADNQDRDFESNVTIALGVFEPMLVVSMAAVVLFIVLAILQPILA
LNNMISG

Sequences:

>Translated_407_residues
MPAFEYKALDKTGKQKKGVVEADTARHARTQLREQRLMPLEITPVVEKESKTKAAGFSFLKRGISTAELALITRQIATLV
AAGLPVEEALKAVGQQCEKDRLASMVMAVRSRVVEGYSLADSMAEFPHIFDELYRAMVASGEKSGHLEVVLNRLADYTER
RQQLKSKMTQAMIYPIVLTVVAIGVIAILLAAVVPQVVGQFEHMGQELPWTTELLIASSDFIRDYGLIVLAVIVGLFFIA
KRLLVSPKNRMKYDSMLLRLPVISKVSKSLNTARFARTLSILTASSVPLLDAMRIASDVLINVKVKAAVEDATLRVREGT
SLGTALANTKLFPPMMLYMITSGEKSGQLEQMLERAADNQDRDFESNVTIALGVFEPMLVVSMAAVVLFIVLAILQPILA
LNNMISG
>Mature_406_residues
PAFEYKALDKTGKQKKGVVEADTARHARTQLREQRLMPLEITPVVEKESKTKAAGFSFLKRGISTAELALITRQIATLVA
AGLPVEEALKAVGQQCEKDRLASMVMAVRSRVVEGYSLADSMAEFPHIFDELYRAMVASGEKSGHLEVVLNRLADYTERR
QQLKSKMTQAMIYPIVLTVVAIGVIAILLAAVVPQVVGQFEHMGQELPWTTELLIASSDFIRDYGLIVLAVIVGLFFIAK
RLLVSPKNRMKYDSMLLRLPVISKVSKSLNTARFARTLSILTASSVPLLDAMRIASDVLINVKVKAAVEDATLRVREGTS
LGTALANTKLFPPMMLYMITSGEKSGQLEQMLERAADNQDRDFESNVTIALGVFEPMLVVSMAAVVLFIVLAILQPILAL
NNMISG

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=407, Percent_Identity=44.7174447174447, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1786295, Length=413, Percent_Identity=22.7602905569007, Blast_Score=119, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 44679; Mature: 44548

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAFEYKALDKTGKQKKGVVEADTARHARTQLREQRLMPLEITPVVEKESKTKAAGFSFL
CCCCHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHH
KRGISTAELALITRQIATLVAAGLPVEEALKAVGQQCEKDRLASMVMAVRSRVVEGYSLA
HHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DSMAEFPHIFDELYRAMVASGEKSGHLEVVLNRLADYTERRQQLKSKMTQAMIYPIVLTV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAIGVIAILLAAVVPQVVGQFEHMGQELPWTTELLIASSDFIRDYGLIVLAVIVGLFFIA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRLLVSPKNRMKYDSMLLRLPVISKVSKSLNTARFARTLSILTASSVPLLDAMRIASDVL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
INVKVKAAVEDATLRVREGTSLGTALANTKLFPPMMLYMITSGEKSGQLEQMLERAADNQ
EEEEEHEEHHHHHEEEECCCCHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCC
DRDFESNVTIALGVFEPMLVVSMAAVVLFIVLAILQPILALNNMISG
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PAFEYKALDKTGKQKKGVVEADTARHARTQLREQRLMPLEITPVVEKESKTKAAGFSFL
CCCHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHH
KRGISTAELALITRQIATLVAAGLPVEEALKAVGQQCEKDRLASMVMAVRSRVVEGYSLA
HHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DSMAEFPHIFDELYRAMVASGEKSGHLEVVLNRLADYTERRQQLKSKMTQAMIYPIVLTV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAIGVIAILLAAVVPQVVGQFEHMGQELPWTTELLIASSDFIRDYGLIVLAVIVGLFFIA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRLLVSPKNRMKYDSMLLRLPVISKVSKSLNTARFARTLSILTASSVPLLDAMRIASDVL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
INVKVKAAVEDATLRVREGTSLGTALANTKLFPPMMLYMITSGEKSGQLEQMLERAADNQ
EEEEEHEEHHHHHEEEECCCCHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCC
DRDFESNVTIALGVFEPMLVVSMAAVVLFIVLAILQPILALNNMISG
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1640836 [H]