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The map label for this gene is epsE [H]

Identifier: 157959975

GI number: 157959975

Start: 161916

End: 163487

Strand: Direct

Name: epsE [H]

Synonym: Spea_0145

Alternate gene names: 157959975

Gene position: 161916-163487 (Clockwise)

Preceding gene: 157959974

Following gene: 157959976

Centisome position: 3.13

GC content: 47.07

Gene sequence:

>1572_bases
ATGAGTGAGAATTCAATTATAGTCGATGAAGAGCTAGTGCAGGTCTCGAATGAGCTTGGACTAGTGTCTGAGGAAGAGGC
GGATCAGCTTTTTCATTCGAATAGCAAAGAGCGTTTGCCCTTTGCTTTTGCCCATAGATTTAAGGTGGCGCTTGCCGCCG
ACGCTGACGGCGAGTTGTCGCTTTACCATACTCAAGGTACACCATTGAGTGCATTGCTTGAGGTACGTCGCTACTGTGGT
AAAGAATTGCCCTTGATAACGTTAGAAGACACAGCTTTTGAGGCGCGTCTTACTCAAGCCTATCAAGCTAACTCTTCTGA
AGCGCAGCAGATGATGGAAGATATCGGTAATGAAATGGACCTGTTCACTCTTGCCGAAGAGTTGCCACAAACAGAAGATC
TACTCGAGGGTGACGATGACGCACCGATTATTAAGTTAATCAATGCGCTATTGTCTGAGGCTATTAAGGAAGAAGCGTCA
GATATTCATATCGAAACCTACGAGAAACAGTTAATTGTGCGTTTCCGTGTCGATGGCATTCTCAAAGAAGTTCTCAAGCC
TAATCGTAAACTGTCATCTTTGTTGGTGTCACGTATCAAGGTTATGGCACGTCTCGATATTGCTGAAAAACGTGTACCAC
AAGATGGTCGCATTTCGCTGCGTATCGCGGGACGAGCTGTGGATGTGCGTGTATCGACTATGCCGTCAAGTCATGGCGAG
CGTGTGGTGCTACGTCTACTCGATAAAAATGCGGGTAACTTAGACCTAGAGCAGCTAGGTATGACCGAAGCGGTTCGTCA
TCAATTTGATGACTTAATCCGCAGACCACACGGTATTATTTTGGTGACCGGTCCAACCGGTTCGGGTAAGAGTACCACTC
TCTATGCAGGCCTTACCGAAATTAACTCGAAAGATACCAACATTCTTACCGTCGAAGACCCAATAGAATACGAACTCGAA
GGTATTGGTCAGACTCAGGTAAACACTAAGGTCGATATGACCTTCGCTCGTGGCCTACGTGCTATTTTGCGTCAAGATCC
TGATGTGGTGATGATTGGTGAGATCCGAGATCTTGAGACTGCGCAAATTGCGGTTCAGGCATCGTTAACTGGCCATATGG
TTATCTCAACCCTACATACTAATACGGCATCGGGCGCGATTACTCGTTTACAAGATATGGGCGTAGAACCTTTCCTCGTG
TCTTCCAGTTTACTTGGTGTCCTCGCTCAACGTCTTATCCGTACCTTGTGCCCAAGCTGTAAAGAGTCGCATACCCCGGA
TAATCGTGAAAGAGAGCTTCTCGGTATTACCGCTGACGATCCTCGTGTTATTTTCCGCGCGAAGGGTTGTCAGGCTTGTG
GTCACAATGGTTATCGTGGCCGTACCGGTATTCATGAGTTACTCATAGTCGATGATCATGTGCGTGAACTTATTCACACC
GGTCGCGGTGAATTAGCGATTGAAAAGCATATTCGTCAATCTGTTCCGAGCATTCGTCACGACGGTATGAGTAAAGTGCT
TGCGGGGAAAACTACCCTAGAAGAAGTGCTGCGTGTCACTCGAGAGGAGTAA

Upstream 100 bases:

>100_bases
TTAGACACAAAGATGCGTGAAACCGATCCGGCGCTAAAGTCTATCTCGAATAGCAAAGATGCCGATAAGCAAGGCACTGA
TACAGATAAAGAAACTTCTG

Downstream 100 bases:

>100_bases
GCCATGCCTGCATTTGAATACAAAGCATTAGACAAAACGGGTAAGCAGAAAAAAGGTGTTGTCGAAGCGGATACCGCAAG
GCATGCACGTACTCAGCTAA

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MSENSIIVDEELVQVSNELGLVSEEEADQLFHSNSKERLPFAFAHRFKVALAADADGELSLYHTQGTPLSALLEVRRYCG
KELPLITLEDTAFEARLTQAYQANSSEAQQMMEDIGNEMDLFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEAS
DIHIETYEKQLIVRFRVDGILKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGE
RVVLRLLDKNAGNLDLEQLGMTEAVRHQFDDLIRRPHGIILVTGPTGSGKSTTLYAGLTEINSKDTNILTVEDPIEYELE
GIGQTQVNTKVDMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHMVISTLHTNTASGAITRLQDMGVEPFLV
SSSLLGVLAQRLIRTLCPSCKESHTPDNRERELLGITADDPRVIFRAKGCQACGHNGYRGRTGIHELLIVDDHVRELIHT
GRGELAIEKHIRQSVPSIRHDGMSKVLAGKTTLEEVLRVTREE

Sequences:

>Translated_523_residues
MSENSIIVDEELVQVSNELGLVSEEEADQLFHSNSKERLPFAFAHRFKVALAADADGELSLYHTQGTPLSALLEVRRYCG
KELPLITLEDTAFEARLTQAYQANSSEAQQMMEDIGNEMDLFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEAS
DIHIETYEKQLIVRFRVDGILKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGE
RVVLRLLDKNAGNLDLEQLGMTEAVRHQFDDLIRRPHGIILVTGPTGSGKSTTLYAGLTEINSKDTNILTVEDPIEYELE
GIGQTQVNTKVDMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHMVISTLHTNTASGAITRLQDMGVEPFLV
SSSLLGVLAQRLIRTLCPSCKESHTPDNRERELLGITADDPRVIFRAKGCQACGHNGYRGRTGIHELLIVDDHVRELIHT
GRGELAIEKHIRQSVPSIRHDGMSKVLAGKTTLEEVLRVTREE
>Mature_522_residues
SENSIIVDEELVQVSNELGLVSEEEADQLFHSNSKERLPFAFAHRFKVALAADADGELSLYHTQGTPLSALLEVRRYCGK
ELPLITLEDTAFEARLTQAYQANSSEAQQMMEDIGNEMDLFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASD
IHIETYEKQLIVRFRVDGILKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGER
VVLRLLDKNAGNLDLEQLGMTEAVRHQFDDLIRRPHGIILVTGPTGSGKSTTLYAGLTEINSKDTNILTVEDPIEYELEG
IGQTQVNTKVDMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHMVISTLHTNTASGAITRLQDMGVEPFLVS
SSLLGVLAQRLIRTLCPSCKESHTPDNRERELLGITADDPRVIFRAKGCQACGHNGYRGRTGIHELLIVDDHVRELIHTG
RGELAIEKHIRQSVPSIRHDGMSKVLAGKTTLEEVLRVTREE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=479, Percent_Identity=57.2025052192067, Blast_Score=556, Evalue=1e-159,
Organism=Escherichia coli, GI1786296, Length=384, Percent_Identity=43.2291666666667, Blast_Score=318, Evalue=7e-88,
Organism=Escherichia coli, GI87082188, Length=169, Percent_Identity=45.5621301775148, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 58017; Mature: 57886

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENSIIVDEELVQVSNELGLVSEEEADQLFHSNSKERLPFAFAHRFKVALAADADGELS
CCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHEEEEEEECCCCCEE
LYHTQGTPLSALLEVRRYCGKELPLITLEDTAFEARLTQAYQANSSEAQQMMEDIGNEMD
EEEECCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHH
LFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDIHIETYEKQLIVRFRVDGI
HHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHEEEEHHHHHH
LKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECEEEEEEEEECCCCCCH
RVVLRLLDKNAGNLDLEQLGMTEAVRHQFDDLIRRPHGIILVTGPTGSGKSTTLYAGLTE
HHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHH
INSKDTNILTVEDPIEYELEGIGQTQVNTKVDMTFARGLRAILRQDPDVVMIGEIRDLET
CCCCCCEEEEECCCCCHHHCCCCCEECCCEEHHHHHHHHHHHHHCCCCEEEECCCCCCCH
AQIAVQASLTGHMVISTLHTNTASGAITRLQDMGVEPFLVSSSLLGVLAQRLIRTLCPSC
HEEEEEEECCCEEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCH
KESHTPDNRERELLGITADDPRVIFRAKGCQACGHNGYRGRTGIHELLIVDDHVRELIHT
HCCCCCCCCCCEEEEEECCCCEEEEEECCCHHCCCCCCCCCCCCCEEEEECHHHHHHHHC
GRGELAIEKHIRQSVPSIRHDGMSKVLAGKTTLEEVLRVTREE
CCCCHHHHHHHHHHCCHHHHCCHHHHHHCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SENSIIVDEELVQVSNELGLVSEEEADQLFHSNSKERLPFAFAHRFKVALAADADGELS
CCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHEEEEEEECCCCCEE
LYHTQGTPLSALLEVRRYCGKELPLITLEDTAFEARLTQAYQANSSEAQQMMEDIGNEMD
EEEECCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHH
LFTLAEELPQTEDLLEGDDDAPIIKLINALLSEAIKEEASDIHIETYEKQLIVRFRVDGI
HHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHEEEEHHHHHH
LKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIAGRAVDVRVSTMPSSHGE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECEEEEEEEEECCCCCCH
RVVLRLLDKNAGNLDLEQLGMTEAVRHQFDDLIRRPHGIILVTGPTGSGKSTTLYAGLTE
HHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHH
INSKDTNILTVEDPIEYELEGIGQTQVNTKVDMTFARGLRAILRQDPDVVMIGEIRDLET
CCCCCCEEEEECCCCCHHHCCCCCEECCCEEHHHHHHHHHHHHHCCCCEEEECCCCCCCH
AQIAVQASLTGHMVISTLHTNTASGAITRLQDMGVEPFLVSSSLLGVLAQRLIRTLCPSC
HEEEEEEECCCEEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCH
KESHTPDNRERELLGITADDPRVIFRAKGCQACGHNGYRGRTGIHELLIVDDHVRELIHT
HCCCCCCCCCCEEEEEECCCCEEEEEECCCHHCCCCCCCCCCCCCEEEEECHHHHHHHHC
GRGELAIEKHIRQSVPSIRHDGMSKVLAGKTTLEEVLRVTREE
CCCCHHHHHHHHHHCCHHHHCCHHHHHHCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]