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The map label for this gene is dapE [H]

Identifier: 157826361

GI number: 157826361

Start: 1212663

End: 1213784

Strand: Reverse

Name: dapE [H]

Synonym: A1C_06750

Alternate gene names: 157826361

Gene position: 1213784-1212663 (Counterclockwise)

Preceding gene: 157826362

Following gene: 157826359

Centisome position: 98.6

GC content: 33.16

Gene sequence:

>1122_bases
TTGATTGGTTTTAAGTCTGTAACTCCTGAAAACGATGGTGCTATTGAGTATATTGATGATTTGCTTAAACAGCACGGCTT
TAAGACCGAAATAAAAATATTCGGCGATTTTAAAAGCGAGCAAGTCACTAACCTTTATGCTATTTTTGGTAGAAATGAGC
CTAATATTTGCTTTGTTGGTCATGTAGATGTAGTGCCGGTAGATAATCATGCGCTTTGGCATAATTCGAGTCCATTTAAG
GCTAGCAAGCAAGATGGTAAGATATATGGCAGAGGTGCGGTGGATATGAAAGGGGCTATAGCGTGCTTTCTAGCAGCTAG
CTTAGATTTTATAAAAAATAATGCCGGTTTTAAAGGTTCTATTAGTTTCTTACTTACTAGTGATGAAGAGGGGAAAGCAA
AGCATGGCACTAAAGAAATGCTGCAATATATTTATGACCAGGGATATAAGGTAGATTTTGCTATTGTCGGTGAACCTACT
TGTGAAAAAGAAATAGGCGATACAATTAAGATCGGCCGAAGAGGAAGTGTTAACTTTAAATTAAATATAGACGGTTTAGC
AGGGCATGTAGCTTATCCTCATAAAGCAAATAATCCGTTGCCTTGCTTAATAAAAATATTGCATGAGTTAACGAATATAA
GACTTGATGAAGGTACTGAATTTTTTCAAAATTCAAATCTTGAAGTAACGAATATTGATGTTGGTAATGAGACCTCAAAT
GTAATTCCGGCAAGTACTGAAGCATCTTTCAATATACGTTTTAATAATTTACATAGTGCAGAAACTTTAGCAAAACAGGT
AGAAGAAATTATTAAACGATATTGCAAAGAATATAAGGTAGATTATAAGTTAGAATATAGTAGTTTTGCTGGAAGTTTTA
TTCAGAATCCTAGTGCTAAAATAAAAGAATTTGCTAAAGTAGTAGAGCATACTCTTAAAATAAAACCTAAATTCTCTACA
AGCGGCGGTACGTCAGATGCAAGATTCGTCAAAAATTATTGTCCGTTAGTAGAATTCGGTTTATTATCCGAAACGGCACA
TAAAATAAACGAATACACAAAAATTAGCGATTTACAAAAATTATATGATGTGTATTATAATTTTTTGATTAAAATACTTT
GA

Upstream 100 bases:

>100_bases
TACACTAAGAGTAGATGAGTTGTTTTTATTTTATACTAATAATGAAGCTAAATTAAATTCACTTTGGCAATGATTGATGT
ACATTCACTACTTAAAAGAT

Downstream 100 bases:

>100_bases
TGTCATTGTTGTGTGGATCAGAAAATGTCATTGCGAGCAGCCGTAGGCTGTGCGGCAATCTCAAAAATAATAACAAATTC
CTGAGATTGCGGACGTACAA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MIGFKSVTPENDGAIEYIDDLLKQHGFKTEIKIFGDFKSEQVTNLYAIFGRNEPNICFVGHVDVVPVDNHALWHNSSPFK
ASKQDGKIYGRGAVDMKGAIACFLAASLDFIKNNAGFKGSISFLLTSDEEGKAKHGTKEMLQYIYDQGYKVDFAIVGEPT
CEKEIGDTIKIGRRGSVNFKLNIDGLAGHVAYPHKANNPLPCLIKILHELTNIRLDEGTEFFQNSNLEVTNIDVGNETSN
VIPASTEASFNIRFNNLHSAETLAKQVEEIIKRYCKEYKVDYKLEYSSFAGSFIQNPSAKIKEFAKVVEHTLKIKPKFST
SGGTSDARFVKNYCPLVEFGLLSETAHKINEYTKISDLQKLYDVYYNFLIKIL

Sequences:

>Translated_373_residues
MIGFKSVTPENDGAIEYIDDLLKQHGFKTEIKIFGDFKSEQVTNLYAIFGRNEPNICFVGHVDVVPVDNHALWHNSSPFK
ASKQDGKIYGRGAVDMKGAIACFLAASLDFIKNNAGFKGSISFLLTSDEEGKAKHGTKEMLQYIYDQGYKVDFAIVGEPT
CEKEIGDTIKIGRRGSVNFKLNIDGLAGHVAYPHKANNPLPCLIKILHELTNIRLDEGTEFFQNSNLEVTNIDVGNETSN
VIPASTEASFNIRFNNLHSAETLAKQVEEIIKRYCKEYKVDYKLEYSSFAGSFIQNPSAKIKEFAKVVEHTLKIKPKFST
SGGTSDARFVKNYCPLVEFGLLSETAHKINEYTKISDLQKLYDVYYNFLIKIL
>Mature_373_residues
MIGFKSVTPENDGAIEYIDDLLKQHGFKTEIKIFGDFKSEQVTNLYAIFGRNEPNICFVGHVDVVPVDNHALWHNSSPFK
ASKQDGKIYGRGAVDMKGAIACFLAASLDFIKNNAGFKGSISFLLTSDEEGKAKHGTKEMLQYIYDQGYKVDFAIVGEPT
CEKEIGDTIKIGRRGSVNFKLNIDGLAGHVAYPHKANNPLPCLIKILHELTNIRLDEGTEFFQNSNLEVTNIDVGNETSN
VIPASTEASFNIRFNNLHSAETLAKQVEEIIKRYCKEYKVDYKLEYSSFAGSFIQNPSAKIKEFAKVVEHTLKIKPKFST
SGGTSDARFVKNYCPLVEFGLLSETAHKINEYTKISDLQKLYDVYYNFLIKIL

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=374, Percent_Identity=35.0267379679144, Blast_Score=245, Evalue=3e-66,
Organism=Escherichia coli, GI1790395, Length=355, Percent_Identity=25.3521126760563, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24649210, Length=331, Percent_Identity=26.2839879154079, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24649202, Length=292, Percent_Identity=24.3150684931507, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24649204, Length=292, Percent_Identity=24.3150684931507, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 41818; Mature: 41818

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGFKSVTPENDGAIEYIDDLLKQHGFKTEIKIFGDFKSEQVTNLYAIFGRNEPNICFVG
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHEEEEEEEECCCCCEEEEE
HVDVVPVDNHALWHNSSPFKASKQDGKIYGRGAVDMKGAIACFLAASLDFIKNNAGFKGS
CEEEEECCCCEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEE
ISFLLTSDEEGKAKHGTKEMLQYIYDQGYKVDFAIVGEPTCEKEIGDTIKIGRRGSVNFK
EEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHCCCEEEECCCCCEEEE
LNIDGLAGHVAYPHKANNPLPCLIKILHELTNIRLDEGTEFFQNSNLEVTNIDVGNETSN
EEECCCCCCEECCCCCCCCHHHHHHHHHHHHCCEECCCHHHHHCCCCEEEEEECCCCCCC
VIPASTEASFNIRFNNLHSAETLAKQVEEIIKRYCKEYKVDYKLEYSSFAGSFIQNPSAK
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHH
IKEFAKVVEHTLKIKPKFSTSGGTSDARFVKNYCPLVEFGLLSETAHKINEYTKISDLQK
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LYDVYYNFLIKIL
HHHHHHHHHHHCC
>Mature Secondary Structure
MIGFKSVTPENDGAIEYIDDLLKQHGFKTEIKIFGDFKSEQVTNLYAIFGRNEPNICFVG
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHEEEEEEEECCCCCEEEEE
HVDVVPVDNHALWHNSSPFKASKQDGKIYGRGAVDMKGAIACFLAASLDFIKNNAGFKGS
CEEEEECCCCEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEE
ISFLLTSDEEGKAKHGTKEMLQYIYDQGYKVDFAIVGEPTCEKEIGDTIKIGRRGSVNFK
EEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHCCCEEEECCCCCEEEE
LNIDGLAGHVAYPHKANNPLPCLIKILHELTNIRLDEGTEFFQNSNLEVTNIDVGNETSN
EEECCCCCCEECCCCCCCCHHHHHHHHHHHHCCEECCCHHHHHCCCCEEEEEECCCCCCC
VIPASTEASFNIRFNNLHSAETLAKQVEEIIKRYCKEYKVDYKLEYSSFAGSFIQNPSAK
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHH
IKEFAKVVEHTLKIKPKFSTSGGTSDARFVKNYCPLVEFGLLSETAHKINEYTKISDLQK
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LYDVYYNFLIKIL
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA