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The map label for this gene is miaB [H]

Identifier: 157826266

GI number: 157826266

Start: 1123053

End: 1124390

Strand: Reverse

Name: miaB [H]

Synonym: A1C_06245

Alternate gene names: 157826266

Gene position: 1124390-1123053 (Counterclockwise)

Preceding gene: 157826267

Following gene: 157826265

Centisome position: 91.34

GC content: 35.35

Gene sequence:

>1338_bases
ATGAGTAAGAATCTTTATATTAAGACCTACGGATGTCAGATGAATGTTTACGACTCTGTTAAGATGCAAGATTTGCTTTA
TCCTTTTGGTTATGAACCTACGGAAAATATTGAAGAAGCTGATGTTATTATCCTAAATACCTGTCATATCAGAGAGAAAG
CAGCCGAGAAAACTTATTCTGAACTCGGTCGTATTAAGAAACTACAAGATACGAGAAAAAAACAAGGATTAAACTCTGCA
ATAATAGTTGTAGCAGGTTGTGTTGCTCAGGCAGAGGGAGAAGAAATTTTTACAAGAACTCCTTATGTCGATATTGTTGT
CGGTCCGCAATCTTATTACAACCTACCGGAATTAATCTCAAAAATCGTTAGACACGCAAAACATTTAATCGATCTTGATT
TTGTTGAAGAAGCGAAATTTGATAATTTACCGGAACAATTATATCCGCAAGGAGCTAGTAGCTTTATATCGGTGCAAGAG
GGGTGCGATAAATTTTGCACTTTCTGCGTAGTACCTTATACAAGAGGAGCTGAATTTTCAAGGAACGTAGAACAGGTTTA
CAGAGAAGCATTACAAGTAGTTAGCGGCGGTGCTAAAGAAATTATGCTACTTGGACAAAATGTAAATGCATATAACTGGA
AAGGTTCTGCAGATAAAATATTTAGTTTAGCCGATTTACTCAAACATCTAGCACAAATTCCAAATTTAGAGAGATTGCGT
TATATGACATCGCACCCGATAGATATGACAGATGATCTAATACAACTGCACGGTACTGAGCCTAAATTAATGCCGTTTTT
ACATCTACCGGTGCAGTCAGGTTCAAATAAAATATTAAAAGCAATGAACCGCAAACATGATCGGGAATATTATTTTGATA
TAATTAATCGCTTAAGAGAAGCAAGGCCTGATATAGTTTTATCCTCTGATTTTATTGTCGGTTTTCCTGGTGAAACAGAT
GAAGATTTTGAGGATACTTTAGATTTGGTCCGGAGAGTAAAATACGGTCAGTGCTATTCGTTTAAATATAGCCCACGCCC
CGGTACGCCGGGTGCAACTAGAACCGACCAAATACCTGAACATATAAAATCAGAGAGATTAACTATATTGCAAAAAGAAC
TCATGGATCAGCAATTAGCTTGTAATGAAAGTTGTGTCGGTAGTACTATAAAAGTTCTATTTGATCGTAGCGGTAAATTC
GATGATCAAATAATAGGTAAAACTATATATATGCAGTCTGTATATATCCAAAACCCTAATAAATCTTTACTTGGTAAAAT
CATTGATGTAAAAATTACTAAAGCTTCACTTAATAGTTTAACCGGCGAAATATTATAA

Upstream 100 bases:

>100_bases
TTAAGTTAAGAAGAGCAGATGGATAGTGTTTGCGGTTTATTTAAAATTTGATAAAAGTACCTAAATATAGTATAGAACAT
TTTATAATATTAAAAATTTT

Downstream 100 bases:

>100_bases
TTCATGTATAAATCTCTGTTTTTTTGTTTAAAAATTTTTGCCGTACTTATTCTAATAGGTTGTGCGGTTGTGTGGTATAT
AATATATCACTATTCACGTG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSKNLYIKTYGCQMNVYDSVKMQDLLYPFGYEPTENIEEADVIILNTCHIREKAAEKTYSELGRIKKLQDTRKKQGLNSA
IIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELISKIVRHAKHLIDLDFVEEAKFDNLPEQLYPQGASSFISVQE
GCDKFCTFCVVPYTRGAEFSRNVEQVYREALQVVSGGAKEIMLLGQNVNAYNWKGSADKIFSLADLLKHLAQIPNLERLR
YMTSHPIDMTDDLIQLHGTEPKLMPFLHLPVQSGSNKILKAMNRKHDREYYFDIINRLREARPDIVLSSDFIVGFPGETD
EDFEDTLDLVRRVKYGQCYSFKYSPRPGTPGATRTDQIPEHIKSERLTILQKELMDQQLACNESCVGSTIKVLFDRSGKF
DDQIIGKTIYMQSVYIQNPNKSLLGKIIDVKITKASLNSLTGEIL

Sequences:

>Translated_445_residues
MSKNLYIKTYGCQMNVYDSVKMQDLLYPFGYEPTENIEEADVIILNTCHIREKAAEKTYSELGRIKKLQDTRKKQGLNSA
IIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELISKIVRHAKHLIDLDFVEEAKFDNLPEQLYPQGASSFISVQE
GCDKFCTFCVVPYTRGAEFSRNVEQVYREALQVVSGGAKEIMLLGQNVNAYNWKGSADKIFSLADLLKHLAQIPNLERLR
YMTSHPIDMTDDLIQLHGTEPKLMPFLHLPVQSGSNKILKAMNRKHDREYYFDIINRLREARPDIVLSSDFIVGFPGETD
EDFEDTLDLVRRVKYGQCYSFKYSPRPGTPGATRTDQIPEHIKSERLTILQKELMDQQLACNESCVGSTIKVLFDRSGKF
DDQIIGKTIYMQSVYIQNPNKSLLGKIIDVKITKASLNSLTGEIL
>Mature_444_residues
SKNLYIKTYGCQMNVYDSVKMQDLLYPFGYEPTENIEEADVIILNTCHIREKAAEKTYSELGRIKKLQDTRKKQGLNSAI
IVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELISKIVRHAKHLIDLDFVEEAKFDNLPEQLYPQGASSFISVQEG
CDKFCTFCVVPYTRGAEFSRNVEQVYREALQVVSGGAKEIMLLGQNVNAYNWKGSADKIFSLADLLKHLAQIPNLERLRY
MTSHPIDMTDDLIQLHGTEPKLMPFLHLPVQSGSNKILKAMNRKHDREYYFDIINRLREARPDIVLSSDFIVGFPGETDE
DFEDTLDLVRRVKYGQCYSFKYSPRPGTPGATRTDQIPEHIKSERLTILQKELMDQQLACNESCVGSTIKVLFDRSGKFD
DQIIGKTIYMQSVYIQNPNKSLLGKIIDVKITKASLNSLTGEIL

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=483, Percent_Identity=34.9896480331263, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI28872782, Length=483, Percent_Identity=34.9896480331263, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI93277076, Length=452, Percent_Identity=28.3185840707965, Blast_Score=172, Evalue=5e-43,
Organism=Escherichia coli, GI1786882, Length=447, Percent_Identity=46.9798657718121, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1787057, Length=450, Percent_Identity=30, Blast_Score=192, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI17553146, Length=421, Percent_Identity=33.4916864608076, Blast_Score=244, Evalue=8e-65,
Organism=Caenorhabditis elegans, GI71996771, Length=361, Percent_Identity=25.7617728531856, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21356207, Length=483, Percent_Identity=31.055900621118, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI19922432, Length=456, Percent_Identity=28.9473684210526, Blast_Score=165, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50584; Mature: 50452

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNLYIKTYGCQMNVYDSVKMQDLLYPFGYEPTENIEEADVIILNTCHIREKAAEKTYS
CCCCEEEEEECCEEECCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHH
ELGRIKKLQDTRKKQGLNSAIIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELIS
HHHHHHHHHHHHHHCCCCCEEEHEEHHHHHCCCCCEEEECCEEEEEECCHHHCCHHHHHH
KIVRHAKHLIDLDFVEEAKFDNLPEQLYPQGASSFISVQEGCDKFCTFCVVPYTRGAEFS
HHHHHHHHHHCHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHEEECCCCCCCHHH
RNVEQVYREALQVVSGGAKEIMLLGQNVNAYNWKGSADKIFSLADLLKHLAQIPNLERLR
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHH
YMTSHPIDMTDDLIQLHGTEPKLMPFLHLPVQSGSNKILKAMNRKHDREYYFDIINRLRE
HHHCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
ARPDIVLSSDFIVGFPGETDEDFEDTLDLVRRVKYGQCYSFKYSPRPGTPGATRTDQIPE
CCCCEEEECCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHH
HIKSERLTILQKELMDQQLACNESCVGSTIKVLFDRSGKFDDQIIGKTIYMQSVYIQNPN
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHEEEEEEEEEECCC
KSLLGKIIDVKITKASLNSLTGEIL
HHHHHHHHEEEEEHHHHHHHHCCCC
>Mature Secondary Structure 
SKNLYIKTYGCQMNVYDSVKMQDLLYPFGYEPTENIEEADVIILNTCHIREKAAEKTYS
CCCEEEEEECCEEECCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHH
ELGRIKKLQDTRKKQGLNSAIIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELIS
HHHHHHHHHHHHHHCCCCCEEEHEEHHHHHCCCCCEEEECCEEEEEECCHHHCCHHHHHH
KIVRHAKHLIDLDFVEEAKFDNLPEQLYPQGASSFISVQEGCDKFCTFCVVPYTRGAEFS
HHHHHHHHHHCHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHEEECCCCCCCHHH
RNVEQVYREALQVVSGGAKEIMLLGQNVNAYNWKGSADKIFSLADLLKHLAQIPNLERLR
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHH
YMTSHPIDMTDDLIQLHGTEPKLMPFLHLPVQSGSNKILKAMNRKHDREYYFDIINRLRE
HHHCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
ARPDIVLSSDFIVGFPGETDEDFEDTLDLVRRVKYGQCYSFKYSPRPGTPGATRTDQIPE
CCCCEEEECCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHH
HIKSERLTILQKELMDQQLACNESCVGSTIKVLFDRSGKFDDQIIGKTIYMQSVYIQNPN
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHEEEEEEEEEECCC
KSLLGKIIDVKITKASLNSLTGEIL
HHHHHHHHEEEEEHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA