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The map label for this gene is metK

Identifier: 157826229

GI number: 157826229

Start: 1072655

End: 1073797

Strand: Direct

Name: metK

Synonym: A1C_06040

Alternate gene names: 157826229

Gene position: 1072655-1073797 (Clockwise)

Preceding gene: 157826228

Following gene: 157826230

Centisome position: 87.13

GC content: 39.02

Gene sequence:

>1143_bases
ATGAAAAATTTTATATTCACATCTGAATCAGTTTCTGAAGGACATCCGGACAAAATGGCCGATCAAATTTCAGATACAGT
ACTTGATGAAATTCTAAAGCATGACCCTAGTGGTCGTGTTGCCTGTGAAACATTTGTAACAACCGGTCTAGTTCTAGTGG
GTGGCGAAATTACTACTAGTACCTATGTGGATATTGAGCAAATAGTACGTAGCAAGATACAAGAAATCGGCTATAATAAT
CCAAATTATGGATTTGACGGTAGCTGTTGTGCCGTAATTTCATCCATCATGAAACAATCGCCCGATATAGCTATGGGTGT
TGACAATGCAAATGACGATGAAATAGGTGCAGGAGACCAAGGTATGGTTTTCGGTTATGCATGTAATGAAACAGATAGCT
TAATGCCTGCACCGATTTACTACGCTCATCTTTTAATGAGAAGACAAGCATATTTACGTAAACAAAATGTCTTGCCTTGG
CTTAGACCCGATGCAAAATCACAAGTCACGTTACGATATGAAAATAACAAACCTGTTGCCATTGATGCAGTAGTTTTATC
AACTCAGCATCATCCGGAAATACAACAAAAAGATCTTATTGAAGCAGTAATAGAAGAAATCATTAAACCTACCTTACCAA
GCAGTTGGCTAAATAAAGACACTAAATACTTAATTAACCCGACGGGTAGGTTTGTTATAGGAGGTCCTGTTGCCGATTGC
GGTTTAACCGGTAGGAAAATTATCGTGGACAGCTATGGAGGCATGGCAAGACACGGTGGCGGTTGTTTTTCAGGAAAAGA
TCCAACCAAAATAGATAGGTCTGCTGCTTATATGGCCCGCTATATTGCTAAGAATGTAGTAGGAGCAGGACTTGCCGATC
GATGTGAGATACAAATATCTTATGCAATCGGTGTTGCTGCTCCCGTATCTGTGTACACTGAAACTTTCGGTACTAGCAAA
TTAAGCAACGAACAAATTACTAAACTAATAACACAACATTTTGATATGCGTCCTGGTAAAATTATTAAACATCTTGCACT
TCGCACTCCATGCTATCAAAAAACGGCTTCTTACGGTCATTTCGGCCGTGAAGACGCAGATTTTACATGGGAGAAATTAG
ATAAAGTAGATATATTGAAGTGA

Upstream 100 bases:

>100_bases
ATAGCTATACTTTAAACGGTATAATTAAAAAAGCTTTGAACGAATTTATAAAAATATAAGAGGTTGTTTGCATCAATTAT
TTTAAAAATGAGCGTAATGT

Downstream 100 bases:

>100_bases
GTATACCCCAAAGTGAAATGAAGAGTTGGTATACGCAGATCAACTTGAAAAACAGCAAGGAGTTTGTAAGCAAATGAGTG
GGGCGTACAGAACATCCAAA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKNFIFTSESVSEGHPDKMADQISDTVLDEILKHDPSGRVACETFVTTGLVLVGGEITTSTYVDIEQIVRSKIQEIGYNN
PNYGFDGSCCAVISSIMKQSPDIAMGVDNANDDEIGAGDQGMVFGYACNETDSLMPAPIYYAHLLMRRQAYLRKQNVLPW
LRPDAKSQVTLRYENNKPVAIDAVVLSTQHHPEIQQKDLIEAVIEEIIKPTLPSSWLNKDTKYLINPTGRFVIGGPVADC
GLTGRKIIVDSYGGMARHGGGCFSGKDPTKIDRSAAYMARYIAKNVVGAGLADRCEIQISYAIGVAAPVSVYTETFGTSK
LSNEQITKLITQHFDMRPGKIIKHLALRTPCYQKTASYGHFGREDADFTWEKLDKVDILK

Sequences:

>Translated_380_residues
MKNFIFTSESVSEGHPDKMADQISDTVLDEILKHDPSGRVACETFVTTGLVLVGGEITTSTYVDIEQIVRSKIQEIGYNN
PNYGFDGSCCAVISSIMKQSPDIAMGVDNANDDEIGAGDQGMVFGYACNETDSLMPAPIYYAHLLMRRQAYLRKQNVLPW
LRPDAKSQVTLRYENNKPVAIDAVVLSTQHHPEIQQKDLIEAVIEEIIKPTLPSSWLNKDTKYLINPTGRFVIGGPVADC
GLTGRKIIVDSYGGMARHGGGCFSGKDPTKIDRSAAYMARYIAKNVVGAGLADRCEIQISYAIGVAAPVSVYTETFGTSK
LSNEQITKLITQHFDMRPGKIIKHLALRTPCYQKTASYGHFGREDADFTWEKLDKVDILK
>Mature_380_residues
MKNFIFTSESVSEGHPDKMADQISDTVLDEILKHDPSGRVACETFVTTGLVLVGGEITTSTYVDIEQIVRSKIQEIGYNN
PNYGFDGSCCAVISSIMKQSPDIAMGVDNANDDEIGAGDQGMVFGYACNETDSLMPAPIYYAHLLMRRQAYLRKQNVLPW
LRPDAKSQVTLRYENNKPVAIDAVVLSTQHHPEIQQKDLIEAVIEEIIKPTLPSSWLNKDTKYLINPTGRFVIGGPVADC
GLTGRKIIVDSYGGMARHGGGCFSGKDPTKIDRSAAYMARYIAKNVVGAGLADRCEIQISYAIGVAAPVSVYTETFGTSK
LSNEQITKLITQHFDMRPGKIIKHLALRTPCYQKTASYGHFGREDADFTWEKLDKVDILK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=373, Percent_Identity=52.0107238605898, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI5174529, Length=374, Percent_Identity=50.5347593582888, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI1789311, Length=380, Percent_Identity=67.8947368421053, Blast_Score=545, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI17551082, Length=375, Percent_Identity=50.9333333333333, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17538498, Length=374, Percent_Identity=52.1390374331551, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25145633, Length=382, Percent_Identity=51.3089005235602, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17538494, Length=374, Percent_Identity=51.3368983957219, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32565905, Length=374, Percent_Identity=46.7914438502674, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI32565911, Length=328, Percent_Identity=49.390243902439, Blast_Score=323, Evalue=8e-89,
Organism=Caenorhabditis elegans, GI25145635, Length=336, Percent_Identity=48.5119047619048, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=49.1694352159468, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI32565909, Length=82, Percent_Identity=56.0975609756098, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6320710, Length=382, Percent_Identity=50.261780104712, Blast_Score=382, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6323209, Length=375, Percent_Identity=51.4666666666667, Blast_Score=379, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45552159, Length=380, Percent_Identity=51.0526315789474, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580529, Length=380, Percent_Identity=51.0526315789474, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580537, Length=380, Percent_Identity=51.0526315789474, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580533, Length=380, Percent_Identity=51.0526315789474, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580535, Length=380, Percent_Identity=51.0526315789474, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580531, Length=380, Percent_Identity=51.0526315789474, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580527, Length=380, Percent_Identity=50.2631578947368, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580525, Length=380, Percent_Identity=50.2631578947368, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580541, Length=367, Percent_Identity=49.0463215258856, Blast_Score=345, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24580539, Length=118, Percent_Identity=52.5423728813559, Blast_Score=131, Evalue=7e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_RICAH (A8GPW6)

Other databases:

- EMBL:   CP000847
- RefSeq:   YP_001493949.1
- ProteinModelPortal:   A8GPW6
- SMR:   A8GPW6
- STRING:   A8GPW6
- GeneID:   5645806
- GenomeReviews:   CP000847_GR
- KEGG:   rak:A1C_06040
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   RAKA293614:A1C_06040-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 41884; Mature: 41884

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNFIFTSESVSEGHPDKMADQISDTVLDEILKHDPSGRVACETFVTTGLVLVGGEITTS
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHCEEEECCCCCCH
TYVDIEQIVRSKIQEIGYNNPNYGFDGSCCAVISSIMKQSPDIAMGVDNANDDEIGAGDQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCC
GMVFGYACNETDSLMPAPIYYAHLLMRRQAYLRKQNVLPWLRPDAKSQVTLRYENNKPVA
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEE
IDAVVLSTQHHPEIQQKDLIEAVIEEIIKPTLPSSWLNKDTKYLINPTGRFVIGGPVADC
EEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDSYGGMARHGGGCFSGKDPTKIDRSAAYMARYIAKNVVGAGLADRCEIQIS
CCCCCEEEEECCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
YAIGVAAPVSVYTETFGTSKLSNEQITKLITQHFDMRPGKIIKHLALRTPCYQKTASYGH
EEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCCC
FGREDADFTWEKLDKVDILK
CCCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MKNFIFTSESVSEGHPDKMADQISDTVLDEILKHDPSGRVACETFVTTGLVLVGGEITTS
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHCEEEECCCCCCH
TYVDIEQIVRSKIQEIGYNNPNYGFDGSCCAVISSIMKQSPDIAMGVDNANDDEIGAGDQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCC
GMVFGYACNETDSLMPAPIYYAHLLMRRQAYLRKQNVLPWLRPDAKSQVTLRYENNKPVA
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEE
IDAVVLSTQHHPEIQQKDLIEAVIEEIIKPTLPSSWLNKDTKYLINPTGRFVIGGPVADC
EEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDSYGGMARHGGGCFSGKDPTKIDRSAAYMARYIAKNVVGAGLADRCEIQIS
CCCCCEEEEECCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
YAIGVAAPVSVYTETFGTSKLSNEQITKLITQHFDMRPGKIIKHLALRTPCYQKTASYGH
EEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCCC
FGREDADFTWEKLDKVDILK
CCCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA