Click here to switch to the map view.

The map label for this gene is polA [H]

Identifier: 157826228

GI number: 157826228

Start: 1069423

End: 1072041

Strand: Direct

Name: polA [H]

Synonym: A1C_06035

Alternate gene names: 157826228

Gene position: 1069423-1072041 (Clockwise)

Preceding gene: 157826227

Following gene: 157826229

Centisome position: 86.87

GC content: 32.76

Gene sequence:

>2619_bases
ATGACTCAAAAAAATACTTTATTACTCATAGACGGATACGGATTGGTTTTTAGAGCCTATTATGCACAACAACATTTAAC
TTCACCGAAAGGTGAGCCTGTCGGTGCTCTTTACGGCTTCACTTCAATGCTCCTAAAATTGCTGAGCGACTTTAAGCCTA
AACATGTCGCTGTAGTATTTGACAGCGGCGGGAACAATTTTCGCCATCATATTTATCCTGAATATAAAGCTAACCGCCCG
CCTCCACCTGAGGATCTAGTCGTACAATTACCATTAGTTCGGGATGTCGCTAGTAACTTAAACTTGCCTATTCTAGAAAA
AAACGGCTATGAAGCCGATGACATTATTGCAACCTTTGCCGCTAAAACAGCTGCACTCGGCGAAGATGTAGTTGTCATCT
CTTCCGATAAAGATTTATTACAGTTAATGAGTGAGAACATCAAAATATATGATCCTCTAAAAGGCAAATATATTACAGAG
GATGATATTGTTAAAAAATTCGGTACTACTTCGGATAAATTACGTGAAGTGATGGCTTTAACAGGCGATAGATCGGATAA
TATTCCAGGCGTACCGTCCATTGGACCGAAAACGGCAAGTAGTCTTATAACACAATTTGGTTCTATAGAGAATATATTTA
ACTCTTTAGACCAAGTCTCAAGCGTAAAACAACGTGAAACTTTACAAAATTCTAAGGAAGATGCGTTAATTTCATGGCAG
TTAATCGGACTTGATTCTAACATTGATTTAGATTTTCAGCTGAATAATTTAGAATGGTTACCTCCAAATAGTGATAAATT
AACAGAATTTTTACAAAAATACGGTTTTAAATCTTTATATAAAAGAGCAGAAAATTTATTTGATATTAAAATTAATGATC
ACAAAGAAATAGTAGATAATAAAGTAACTGAAGAAAAAGAAATCAATAATGCTGGCGAGCTTGCGAATTTTGCTAAAGAA
GCTGAGAAAATAGGAATATTCGGGATATATTTAGTGCAGCACAAAGGAGATAATCTTGCTTTTATTTTATCACTGCAAAA
CCAAAGCTATATTATAAAAATATCTAATACTTCTAATGATTTATTTCCTTATAATACTAAAAATAATAACGACTGGTTTT
CAGATATAATCTTTAATTTACTAGCCGATAAATCCATAAAAAAAATTACCTACTCTCTTAAACCTCTACTTAAATTTTAT
GCAAACCAATCTCATAAAATTACGGCTATAGAGGATTTAGAACTAATGCAATATGCGTTATCTGCCGGTTTATCACAGAA
AAATTTATTTGAAGAAGCTTTAAAAGAGGACAATATAATCAATGAATCAGCAAAAATAGTAGCAAATTTTATCTCACTTT
ATAAGCAAACTATATTAGCACTTAAAGATAATAAAGCCTTTAGACTGTATAGCGACATTGATTTACCTATTTGTTTTATC
TTAGATAAAATGGAGAAAACAGGTATAAAAGTAGATGCAAATTATCTAAATCAGTTATCTGCTGAGTTTGGAGCAGAAAT
ATCTATACTTGAAGAAGAAATTTTTGCTCTTAGTGGAACTAAATTTAATATCGGCTCACCCAAACAACTAGGTAAGATTT
TATTTGAAAAAATGCAATTACCTTTTGGTAAAGCTGCGGCTAAAGCAATCTCTTACTCAACAGCTGCTGAAATACTCGAA
AAACTTAGTGAACATGGATATAATATTGCAGATTTATTATTAAGATGGCGGCAACTTACAAAATTCAAGAATACCTACAC
CGATAGTTTGCCGAAACAGATAGATAATATAACAAGGCGAGTACATACGACATTTTTACAAACTTCTACCACCACAGGAA
GACTTAGCTCACAAGAACCTAATCTGCAAAACATACCTATTCGCTCTAGGGAGGGGAATAAAATCAGACAAGCTTTTATT
GCTGAAGAAGGCTATAAATTGATATCTGCAGATTATTCTCAAATTGAGCTTAGAATATTAAGCCATATCGCAAATATAGA
TACACTAAAACAAGCATTTATTAATAAAGACGATATCCATACTCAAACGGCTTGTCAAATCTTTAACTTGCAAAAACATG
AACTAACTAGCGAACATAGACGTAAAGCAAAAGCGATAAATTTTGGTATTATTTACGGTATAAGTGCTTTTGGGCTTGCT
AAGCAATTAAACGTTAGTAACAGCGAAGCATCCGAATATATCAAGAAATATTTTGCCGAATATAAAGGAGTACAAGAATA
TATGGAGCAAACAAAAGCTTTTGCAAGCAGCAACGGCTATGTCATAAATTTCTTTGGCAGAAAATGTTTTATACCTCTAA
TTCATGATAAAAAGCTTAAACAATTTGCAGAACGTGCAGCTATTAATGCTCCTATTCAAGGAACTAATGCCGATATAATT
AAAATTGCTATGATCAATCTAGATCAAGAAATAGCAAAAAATAAGCTTAAAACCAGATTAGTACTGCAAATTCATGATGA
ATTACTATTTGAAGTGCCGGAGGATGAAGTAGAACTCGTAACGCCTATTATCAAAAAAATCATGGAAAACTCTACTAATA
TGGATGTACCGATTATAACCGAAATTAGAGCAGGTAATAACTGGATGGAAATTCATTGA

Upstream 100 bases:

>100_bases
TTCGTTAATTATTTCATAATTTCCTCACAATGACGGAAAGACGCACCACGCAGGCAATATCCACTCGCAATGACGATAGA
ATTCAAAATAAAATCACGAA

Downstream 100 bases:

>100_bases
ATTATATAAAACTGTGTCAAGGCTTGTTGACATTGTTGCGTGGATACTATAGCCGTCATTGCTAGGATCGAAGCGACGTG
GCAATCCAGAAAAATAATTA

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 872; Mature: 871

Protein sequence:

>872_residues
MTQKNTLLLIDGYGLVFRAYYAQQHLTSPKGEPVGALYGFTSMLLKLLSDFKPKHVAVVFDSGGNNFRHHIYPEYKANRP
PPPEDLVVQLPLVRDVASNLNLPILEKNGYEADDIIATFAAKTAALGEDVVVISSDKDLLQLMSENIKIYDPLKGKYITE
DDIVKKFGTTSDKLREVMALTGDRSDNIPGVPSIGPKTASSLITQFGSIENIFNSLDQVSSVKQRETLQNSKEDALISWQ
LIGLDSNIDLDFQLNNLEWLPPNSDKLTEFLQKYGFKSLYKRAENLFDIKINDHKEIVDNKVTEEKEINNAGELANFAKE
AEKIGIFGIYLVQHKGDNLAFILSLQNQSYIIKISNTSNDLFPYNTKNNNDWFSDIIFNLLADKSIKKITYSLKPLLKFY
ANQSHKITAIEDLELMQYALSAGLSQKNLFEEALKEDNIINESAKIVANFISLYKQTILALKDNKAFRLYSDIDLPICFI
LDKMEKTGIKVDANYLNQLSAEFGAEISILEEEIFALSGTKFNIGSPKQLGKILFEKMQLPFGKAAAKAISYSTAAEILE
KLSEHGYNIADLLLRWRQLTKFKNTYTDSLPKQIDNITRRVHTTFLQTSTTTGRLSSQEPNLQNIPIRSREGNKIRQAFI
AEEGYKLISADYSQIELRILSHIANIDTLKQAFINKDDIHTQTACQIFNLQKHELTSEHRRKAKAINFGIIYGISAFGLA
KQLNVSNSEASEYIKKYFAEYKGVQEYMEQTKAFASSNGYVINFFGRKCFIPLIHDKKLKQFAERAAINAPIQGTNADII
KIAMINLDQEIAKNKLKTRLVLQIHDELLFEVPEDEVELVTPIIKKIMENSTNMDVPIITEIRAGNNWMEIH

Sequences:

>Translated_872_residues
MTQKNTLLLIDGYGLVFRAYYAQQHLTSPKGEPVGALYGFTSMLLKLLSDFKPKHVAVVFDSGGNNFRHHIYPEYKANRP
PPPEDLVVQLPLVRDVASNLNLPILEKNGYEADDIIATFAAKTAALGEDVVVISSDKDLLQLMSENIKIYDPLKGKYITE
DDIVKKFGTTSDKLREVMALTGDRSDNIPGVPSIGPKTASSLITQFGSIENIFNSLDQVSSVKQRETLQNSKEDALISWQ
LIGLDSNIDLDFQLNNLEWLPPNSDKLTEFLQKYGFKSLYKRAENLFDIKINDHKEIVDNKVTEEKEINNAGELANFAKE
AEKIGIFGIYLVQHKGDNLAFILSLQNQSYIIKISNTSNDLFPYNTKNNNDWFSDIIFNLLADKSIKKITYSLKPLLKFY
ANQSHKITAIEDLELMQYALSAGLSQKNLFEEALKEDNIINESAKIVANFISLYKQTILALKDNKAFRLYSDIDLPICFI
LDKMEKTGIKVDANYLNQLSAEFGAEISILEEEIFALSGTKFNIGSPKQLGKILFEKMQLPFGKAAAKAISYSTAAEILE
KLSEHGYNIADLLLRWRQLTKFKNTYTDSLPKQIDNITRRVHTTFLQTSTTTGRLSSQEPNLQNIPIRSREGNKIRQAFI
AEEGYKLISADYSQIELRILSHIANIDTLKQAFINKDDIHTQTACQIFNLQKHELTSEHRRKAKAINFGIIYGISAFGLA
KQLNVSNSEASEYIKKYFAEYKGVQEYMEQTKAFASSNGYVINFFGRKCFIPLIHDKKLKQFAERAAINAPIQGTNADII
KIAMINLDQEIAKNKLKTRLVLQIHDELLFEVPEDEVELVTPIIKKIMENSTNMDVPIITEIRAGNNWMEIH
>Mature_871_residues
TQKNTLLLIDGYGLVFRAYYAQQHLTSPKGEPVGALYGFTSMLLKLLSDFKPKHVAVVFDSGGNNFRHHIYPEYKANRPP
PPEDLVVQLPLVRDVASNLNLPILEKNGYEADDIIATFAAKTAALGEDVVVISSDKDLLQLMSENIKIYDPLKGKYITED
DIVKKFGTTSDKLREVMALTGDRSDNIPGVPSIGPKTASSLITQFGSIENIFNSLDQVSSVKQRETLQNSKEDALISWQL
IGLDSNIDLDFQLNNLEWLPPNSDKLTEFLQKYGFKSLYKRAENLFDIKINDHKEIVDNKVTEEKEINNAGELANFAKEA
EKIGIFGIYLVQHKGDNLAFILSLQNQSYIIKISNTSNDLFPYNTKNNNDWFSDIIFNLLADKSIKKITYSLKPLLKFYA
NQSHKITAIEDLELMQYALSAGLSQKNLFEEALKEDNIINESAKIVANFISLYKQTILALKDNKAFRLYSDIDLPICFIL
DKMEKTGIKVDANYLNQLSAEFGAEISILEEEIFALSGTKFNIGSPKQLGKILFEKMQLPFGKAAAKAISYSTAAEILEK
LSEHGYNIADLLLRWRQLTKFKNTYTDSLPKQIDNITRRVHTTFLQTSTTTGRLSSQEPNLQNIPIRSREGNKIRQAFIA
EEGYKLISADYSQIELRILSHIANIDTLKQAFINKDDIHTQTACQIFNLQKHELTSEHRRKAKAINFGIIYGISAFGLAK
QLNVSNSEASEYIKKYFAEYKGVQEYMEQTKAFASSNGYVINFFGRKCFIPLIHDKKLKQFAERAAINAPIQGTNADIIK
IAMINLDQEIAKNKLKTRLVLQIHDELLFEVPEDEVELVTPIIKKIMENSTNMDVPIITEIRAGNNWMEIH

Specific function: In addition to polymerase activity, this DNA polymerase 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RPE1 insert domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=466, Percent_Identity=30.2575107296137, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI139394648, Length=272, Percent_Identity=30.8823529411765, Blast_Score=148, Evalue=2e-35,
Organism=Escherichia coli, GI1790294, Length=930, Percent_Identity=35.6989247311828, Blast_Score=549, Evalue=1e-157,
Organism=Escherichia coli, GI226510970, Length=253, Percent_Identity=32.4110671936759, Blast_Score=141, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI86563272, Length=391, Percent_Identity=29.923273657289, Blast_Score=168, Evalue=1e-41,
Organism=Drosophila melanogaster, GI17933644, Length=426, Percent_Identity=30.2816901408451, Blast_Score=196, Evalue=5e-50,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR005728
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 98689; Mature: 98558

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00447 DNA_POLYMERASE_A ; PS00307 LECTIN_LEGUME_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKNTLLLIDGYGLVFRAYYAQQHLTSPKGEPVGALYGFTSMLLKLLSDFKPKHVAVVF
CCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
DSGGNNFRHHIYPEYKANRPPPPEDLVVQLPLVRDVASNLNLPILEKNGYEADDIIATFA
ECCCCCCCCCCCCCCCCCCCCCCHHHEEECHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
AKTAALGEDVVVISSDKDLLQLMSENIKIYDPLKGKYITEDDIVKKFGTTSDKLREVMAL
HHHHHCCCCEEEEECCHHHHHHHHCCCEEECCCCCCEECHHHHHHHHCCCHHHHHHHHHH
TGDRSDNIPGVPSIGPKTASSLITQFGSIENIFNSLDQVSSVKQRETLQNSKEDALISWQ
CCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LIGLDSNIDLDFQLNNLEWLPPNSDKLTEFLQKYGFKSLYKRAENLFDIKINDHKEIVDN
EEECCCCCEEEEEECCEEECCCCHHHHHHHHHHHCHHHHHHHHCCEEEEEECCHHHHHHC
KVTEEKEINNAGELANFAKEAEKIGIFGIYLVQHKGDNLAFILSLQNQSYIIKISNTSND
CCCCHHHCCCHHHHHHHHHHHHHCCEEEEEEEEECCCCEEEEEEECCCEEEEEEECCCCC
LFPYNTKNNNDWFSDIIFNLLADKSIKKITYSLKPLLKFYANQSHKITAIEDLELMQYAL
CCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
SAGLSQKNLFEEALKEDNIINESAKIVANFISLYKQTILALKDNKAFRLYSDIDLPICFI
HCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCHHHHH
LDKMEKTGIKVDANYLNQLSAEFGAEISILEEEIFALSGTKFNIGSPKQLGKILFEKMQL
HHHHHHCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCC
PFGKAAAKAISYSTAAEILEKLSEHGYNIADLLLRWRQLTKFKNTYTDSLPKQIDNITRR
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHTTFLQTSTTTGRLSSQEPNLQNIPIRSREGNKIRQAFIAEEGYKLISADYSQIELRIL
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCHHHHHHHHH
SHIANIDTLKQAFINKDDIHTQTACQIFNLQKHELTSEHRRKAKAINFGIIYGISAFGLA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHEEHHHHHHHH
KQLNVSNSEASEYIKKYFAEYKGVQEYMEQTKAFASSNGYVINFFGRKCFIPLIHDKKLK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECHHHHH
QFAERAAINAPIQGTNADIIKIAMINLDQEIAKNKLKTRLVLQIHDELLFEVPEDEVELV
HHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TPIIKKIMENSTNMDVPIITEIRAGNNWMEIH
HHHHHHHHCCCCCCCCCEEEEEECCCCEEEEC
>Mature Secondary Structure 
TQKNTLLLIDGYGLVFRAYYAQQHLTSPKGEPVGALYGFTSMLLKLLSDFKPKHVAVVF
CCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
DSGGNNFRHHIYPEYKANRPPPPEDLVVQLPLVRDVASNLNLPILEKNGYEADDIIATFA
ECCCCCCCCCCCCCCCCCCCCCCHHHEEECHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
AKTAALGEDVVVISSDKDLLQLMSENIKIYDPLKGKYITEDDIVKKFGTTSDKLREVMAL
HHHHHCCCCEEEEECCHHHHHHHHCCCEEECCCCCCEECHHHHHHHHCCCHHHHHHHHHH
TGDRSDNIPGVPSIGPKTASSLITQFGSIENIFNSLDQVSSVKQRETLQNSKEDALISWQ
CCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LIGLDSNIDLDFQLNNLEWLPPNSDKLTEFLQKYGFKSLYKRAENLFDIKINDHKEIVDN
EEECCCCCEEEEEECCEEECCCCHHHHHHHHHHHCHHHHHHHHCCEEEEEECCHHHHHHC
KVTEEKEINNAGELANFAKEAEKIGIFGIYLVQHKGDNLAFILSLQNQSYIIKISNTSND
CCCCHHHCCCHHHHHHHHHHHHHCCEEEEEEEEECCCCEEEEEEECCCEEEEEEECCCCC
LFPYNTKNNNDWFSDIIFNLLADKSIKKITYSLKPLLKFYANQSHKITAIEDLELMQYAL
CCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
SAGLSQKNLFEEALKEDNIINESAKIVANFISLYKQTILALKDNKAFRLYSDIDLPICFI
HCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCHHHHH
LDKMEKTGIKVDANYLNQLSAEFGAEISILEEEIFALSGTKFNIGSPKQLGKILFEKMQL
HHHHHHCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCC
PFGKAAAKAISYSTAAEILEKLSEHGYNIADLLLRWRQLTKFKNTYTDSLPKQIDNITRR
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHTTFLQTSTTTGRLSSQEPNLQNIPIRSREGNKIRQAFIAEEGYKLISADYSQIELRIL
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCHHHHHHHHH
SHIANIDTLKQAFINKDDIHTQTACQIFNLQKHELTSEHRRKAKAINFGIIYGISAFGLA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHEEHHHHHHHH
KQLNVSNSEASEYIKKYFAEYKGVQEYMEQTKAFASSNGYVINFFGRKCFIPLIHDKKLK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECHHHHH
QFAERAAINAPIQGTNADIIKIAMINLDQEIAKNKLKTRLVLQIHDELLFEVPEDEVELV
HHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TPIIKKIMENSTNMDVPIITEIRAGNNWMEIH
HHHHHHHHCCCCCCCCCEEEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10486973; 11030655 [H]