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The map label for this gene is nifS1 [H]

Identifier: 157825854

GI number: 157825854

Start: 708766

End: 709881

Strand: Reverse

Name: nifS1 [H]

Synonym: A1C_03965

Alternate gene names: 157825854

Gene position: 709881-708766 (Counterclockwise)

Preceding gene: 157825855

Following gene: 157825853

Centisome position: 57.66

GC content: 32.26

Gene sequence:

>1116_bases
ATGATATACCTAGATCATAATGCAACCACCTTTATCGATCCCAAGGTAAAAGAATATATAATAAGTTTGATGAATAAAGA
GCTTAACCCTTCGTCAATACATAGAGCAGGTGTATTTGCTAGGAATGTTATAGAAGAAGCACGTTTTCAATTGGTTGCGG
CTCTTGGTATAGCCTTATCATCTCGAGAATATGACATTACTTTTACGTCATCGGGAACTGAAAGTAATAATTTAATAATG
AAAAATTTTTATGACGGCGATATTTTTATTTCAGCTATTGAACATTTATCGATCTATAACCATATGGAACACGCTCCAAA
TATTAAAATTATAAGAGTTAACAATCAAGGTTTGGTTGATTTAGAACATCTAGAAGAGTTATTAGCTCAAAGTAACGCTG
CTAAAAAACTAGTTTCTGTAATGATGGCTAATAACGAGAGTGGGGTGCTGCAAGATATAACCACAATAAGTGAAATAGCT
AAAAAATATTATGCAAAATTTCATAGTGATTTAGTACAAAGTTTTGGCAGAATATCTATAAATATCAAAGAGTTGGGATT
AGATTTTGCGACAATTTCAGGGCATAAAATAGGGGGGGGGCATGGTGGTGGTGCTTTAATCTCTAACTCTAACTTTCAAG
TTACTCCTATGATTATAGGAGGAGGACAAGAAAAAGGAATAAGATCAGGTACAGAAAATGTTTTAGCTATTGCAGGGCTT
GGTTTGGCAGCTGAGTTAATAACAAAAGATATCTCAGAAAAATATATAAAAATCAAGAGCTTACAAGAATATTTAGAGCA
AAAATTAAAAAAATATCCTAATGTAAATATTGTGAGTAATAATATCGCAAGATTGCCTAATACTACTTTATTTACTGTAC
AAAATACAGATGCACAAGTGAAATTAATCGGGTTTGATTTACGTAATATTTGCATAAGTTCCGGCTCTGCTTGCTCATCA
GGAAAAATCTCTAAATCACACGTATTAAACAATATGGGTGTAGTGGAAGAAGAGGCAAAATCCTCTATTAGAGTATCTTT
AAGCCATACTAATACAGTAAGTGATATAAAGGCTTTTATAAAAGCCTTTGAGGAAGTGTATAATATACTCGTTTAA

Upstream 100 bases:

>100_bases
AATGTAATTCGCATAAATTATGGAAAGGTCTTGGTAAACATATTAGAGATTATTTTGAAAATATCTCAATTAAAGATACT
CTAAACTTAAATATAAATAT

Downstream 100 bases:

>100_bases
TTTTAGGCTTTTTGTATACAAAAAATTAATTTTATAAACCATATGAACCAACAATTAAACAATTTAACTTTGCCTATATA
TATGGATTATCAGGCAACAA

Product: hypothetical protein

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MIYLDHNATTFIDPKVKEYIISLMNKELNPSSIHRAGVFARNVIEEARFQLVAALGIALSSREYDITFTSSGTESNNLIM
KNFYDGDIFISAIEHLSIYNHMEHAPNIKIIRVNNQGLVDLEHLEELLAQSNAAKKLVSVMMANNESGVLQDITTISEIA
KKYYAKFHSDLVQSFGRISINIKELGLDFATISGHKIGGGHGGGALISNSNFQVTPMIIGGGQEKGIRSGTENVLAIAGL
GLAAELITKDISEKYIKIKSLQEYLEQKLKKYPNVNIVSNNIARLPNTTLFTVQNTDAQVKLIGFDLRNICISSGSACSS
GKISKSHVLNNMGVVEEEAKSSIRVSLSHTNTVSDIKAFIKAFEEVYNILV

Sequences:

>Translated_371_residues
MIYLDHNATTFIDPKVKEYIISLMNKELNPSSIHRAGVFARNVIEEARFQLVAALGIALSSREYDITFTSSGTESNNLIM
KNFYDGDIFISAIEHLSIYNHMEHAPNIKIIRVNNQGLVDLEHLEELLAQSNAAKKLVSVMMANNESGVLQDITTISEIA
KKYYAKFHSDLVQSFGRISINIKELGLDFATISGHKIGGGHGGGALISNSNFQVTPMIIGGGQEKGIRSGTENVLAIAGL
GLAAELITKDISEKYIKIKSLQEYLEQKLKKYPNVNIVSNNIARLPNTTLFTVQNTDAQVKLIGFDLRNICISSGSACSS
GKISKSHVLNNMGVVEEEAKSSIRVSLSHTNTVSDIKAFIKAFEEVYNILV
>Mature_371_residues
MIYLDHNATTFIDPKVKEYIISLMNKELNPSSIHRAGVFARNVIEEARFQLVAALGIALSSREYDITFTSSGTESNNLIM
KNFYDGDIFISAIEHLSIYNHMEHAPNIKIIRVNNQGLVDLEHLEELLAQSNAAKKLVSVMMANNESGVLQDITTISEIA
KKYYAKFHSDLVQSFGRISINIKELGLDFATISGHKIGGGHGGGALISNSNFQVTPMIIGGGQEKGIRSGTENVLAIAGL
GLAAELITKDISEKYIKIKSLQEYLEQKLKKYPNVNIVSNNIARLPNTTLFTVQNTDAQVKLIGFDLRNICISSGSACSS
GKISKSHVLNNMGVVEEEAKSSIRVSLSHTNTVSDIKAFIKAFEEVYNILV

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=30.9178743961353, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI32307132, Length=370, Percent_Identity=32.7027027027027, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI48994898, Length=376, Percent_Identity=32.4468085106383, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1789175, Length=411, Percent_Identity=24.0875912408759, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25143064, Length=377, Percent_Identity=33.42175066313, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17533177, Length=322, Percent_Identity=29.8136645962733, Blast_Score=127, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6319831, Length=383, Percent_Identity=32.6370757180157, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI20129463, Length=371, Percent_Identity=31.8059299191375, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 40694; Mature: 40694

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDHNATTFIDPKVKEYIISLMNKELNPSSIHRAGVFARNVIEEARFQLVAALGIALS
CEEECCCCCEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SREYDITFTSSGTESNNLIMKNFYDGDIFISAIEHLSIYNHMEHAPNIKIIRVNNQGLVD
CCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEE
LEHLEELLAQSNAAKKLVSVMMANNESGVLQDITTISEIAKKYYAKFHSDLVQSFGRISI
HHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
NIKELGLDFATISGHKIGGGHGGGALISNSNFQVTPMIIGGGQEKGIRSGTENVLAIAGL
EEHHHCCEEEEECCCCCCCCCCCCEEEECCCEEEEEEEEECCCCCCCCCCCCCEEEEECC
GLAAELITKDISEKYIKIKSLQEYLEQKLKKYPNVNIVSNNIARLPNTTLFTVQNTDAQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHCCCCEEEEEECCCCEE
KLIGFDLRNICISSGSACSSGKISKSHVLNNMGVVEEEAKSSIRVSLSHTNTVSDIKAFI
EEEEECHHHHHHCCCCCCCCCCCHHHHHHHCCCCCHHHCCCCEEEEEECCCCHHHHHHHH
KAFEEVYNILV
HHHHHHHHHCC
>Mature Secondary Structure
MIYLDHNATTFIDPKVKEYIISLMNKELNPSSIHRAGVFARNVIEEARFQLVAALGIALS
CEEECCCCCEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SREYDITFTSSGTESNNLIMKNFYDGDIFISAIEHLSIYNHMEHAPNIKIIRVNNQGLVD
CCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEE
LEHLEELLAQSNAAKKLVSVMMANNESGVLQDITTISEIAKKYYAKFHSDLVQSFGRISI
HHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
NIKELGLDFATISGHKIGGGHGGGALISNSNFQVTPMIIGGGQEKGIRSGTENVLAIAGL
EEHHHCCEEEEECCCCCCCCCCCCEEEECCCEEEEEEEEECCCCCCCCCCCCCEEEEECC
GLAAELITKDISEKYIKIKSLQEYLEQKLKKYPNVNIVSNNIARLPNTTLFTVQNTDAQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHCCCCEEEEEECCCCEE
KLIGFDLRNICISSGSACSSGKISKSHVLNNMGVVEEEAKSSIRVSLSHTNTVSDIKAFI
EEEEECHHHHHHCCCCCCCCCCCHHHHHHHCCCCCHHHCCCCEEEEEECCCCHHHHHHHH
KAFEEVYNILV
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]