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The map label for this gene is iscS [H]

Identifier: 157825853

GI number: 157825853

Start: 707491

End: 708723

Strand: Reverse

Name: iscS [H]

Synonym: A1C_03960

Alternate gene names: 157825853

Gene position: 708723-707491 (Counterclockwise)

Preceding gene: 157825854

Following gene: 157825852

Centisome position: 57.57

GC content: 38.12

Gene sequence:

>1233_bases
ATGAACCAACAATTAAACAATTTAACTTTGCCTATATATATGGATTATCAGGCAACAACGCCACTAGATCCAAGAGTAAT
GGAGGCAATGTTGCCATATTTTACTACCAAGTTCGGGAATCCTCATTCACGTAGCCATTCTTTTGGCTGGGAAGCAGAAC
GCGCTGTTGAAGAGGCAAGGAGTAGAGTGGCAAGGTTAATAGGAGCAGATACTAAAGAAATTATTTTTACCTCTGGTGCA
ACTGAGTCTAACAACCTTGCAATCAAGGGAGTAGCAAAATTTTACGGCAATAAGAAAAATCATATTATTACCGTAGTCAG
TGAACATAAATGCGTACTTGATGCTTGCAGGTATTTAGAGCAAGAAGGGATAAACATCACATACTTACCGGTTAAACCAA
ATGGAATAATCGCTTTAGAAACTCTAAAAAATGCTATTACCGATCAGACTATGTTAGTTTCAGTTATGGCTGTTAATAAT
GAAATAGGTGTTGTTCAGCCTTTAAAGGAAATCGGGAAAATTTGCCGTGCAAGAGGTGTTTTCTTTCATTCCGATATTGC
TCAAGGTTTTGGTAAAATTCCAATTGATGTTAACGAGTTTAATATTGATCTTGCAAGTATCTCAGGGCATAAAATTTACG
GTCCGAAAGGAATAGGGGGATTATATGTAAGGAAAAAACCTCGGGTTCGTGTTACGCCGCTCATAAACGGTGGTGGACAG
GAGCGAGGTATGCGTTCGGGTACGCTACCTACGCCTTTAATCGTAGGGCTGGGCGTTGCTGCTGAAATAGCGTATAGTGA
GATGGAAAAAGATACTAAGCATGTAAATTATTTATTCGACAGATTTTTAAATAATATACATAGTCGAATTTCGGAAGTTT
ATTTAAATGGTGCTAAAGATCCAAGATATAAAGGCAATCTAAATCTAAGCTTTGCTGGAGTAGAGGGAGAATCAATTATC
CTTGCCATTAAAGATTTAGCGGTTTCTTCTGGTTCTGCTTGTACTTCTGCCTCTTTAGAGCCGTCATATGTTTTACGTTC
CATGGGAATAGGTGAAGAGCTTGCCCATACTTCGATTAGGTTCGGCATCGGTAGATTTACTACCGAGCAGGAAATTGATT
ACGCAGTAAATTTAATATGCTCAAAAATCGATAAATTAAGGAAATTAAGCCCTCTTTGGGAAATGATGCAAGAAGGGATT
GATTTGAAAAAGATTAAATGGGCTGTACATTGA

Upstream 100 bases:

>100_bases
AAGTGATATAAAGGCTTTTATAAAAGCCTTTGAGGAAGTGTATAATATACTCGTTTAATTTTAGGCTTTTTGTATACAAA
AAATTAATTTTATAAACCAT

Downstream 100 bases:

>100_bases
GAATATTAATGTCATCCAGTGATCCCATGCACCGTATGTCATTCCCGCAAAAGCGGGAATGACAAAATTGAAAATAAATT
TTAGAGAATAACAATGGCTT

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNQQLNNLTLPIYMDYQATTPLDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAERAVEEARSRVARLIGADTKEIIFTSGA
TESNNLAIKGVAKFYGNKKNHIITVVSEHKCVLDACRYLEQEGINITYLPVKPNGIIALETLKNAITDQTMLVSVMAVNN
EIGVVQPLKEIGKICRARGVFFHSDIAQGFGKIPIDVNEFNIDLASISGHKIYGPKGIGGLYVRKKPRVRVTPLINGGGQ
ERGMRSGTLPTPLIVGLGVAAEIAYSEMEKDTKHVNYLFDRFLNNIHSRISEVYLNGAKDPRYKGNLNLSFAGVEGESII
LAIKDLAVSSGSACTSASLEPSYVLRSMGIGEELAHTSIRFGIGRFTTEQEIDYAVNLICSKIDKLRKLSPLWEMMQEGI
DLKKIKWAVH

Sequences:

>Translated_410_residues
MNQQLNNLTLPIYMDYQATTPLDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAERAVEEARSRVARLIGADTKEIIFTSGA
TESNNLAIKGVAKFYGNKKNHIITVVSEHKCVLDACRYLEQEGINITYLPVKPNGIIALETLKNAITDQTMLVSVMAVNN
EIGVVQPLKEIGKICRARGVFFHSDIAQGFGKIPIDVNEFNIDLASISGHKIYGPKGIGGLYVRKKPRVRVTPLINGGGQ
ERGMRSGTLPTPLIVGLGVAAEIAYSEMEKDTKHVNYLFDRFLNNIHSRISEVYLNGAKDPRYKGNLNLSFAGVEGESII
LAIKDLAVSSGSACTSASLEPSYVLRSMGIGEELAHTSIRFGIGRFTTEQEIDYAVNLICSKIDKLRKLSPLWEMMQEGI
DLKKIKWAVH
>Mature_410_residues
MNQQLNNLTLPIYMDYQATTPLDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAERAVEEARSRVARLIGADTKEIIFTSGA
TESNNLAIKGVAKFYGNKKNHIITVVSEHKCVLDACRYLEQEGINITYLPVKPNGIIALETLKNAITDQTMLVSVMAVNN
EIGVVQPLKEIGKICRARGVFFHSDIAQGFGKIPIDVNEFNIDLASISGHKIYGPKGIGGLYVRKKPRVRVTPLINGGGQ
ERGMRSGTLPTPLIVGLGVAAEIAYSEMEKDTKHVNYLFDRFLNNIHSRISEVYLNGAKDPRYKGNLNLSFAGVEGESII
LAIKDLAVSSGSACTSASLEPSYVLRSMGIGEELAHTSIRFGIGRFTTEQEIDYAVNLICSKIDKLRKLSPLWEMMQEGI
DLKKIKWAVH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=400, Percent_Identity=64.25, Blast_Score=563, Evalue=1e-161,
Organism=Homo sapiens, GI156713448, Length=412, Percent_Identity=34.2233009708738, Blast_Score=213, Evalue=3e-55,
Organism=Escherichia coli, GI48994898, Length=405, Percent_Identity=60, Blast_Score=503, Evalue=1e-144,
Organism=Escherichia coli, GI1787970, Length=284, Percent_Identity=28.8732394366197, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789175, Length=401, Percent_Identity=26.4339152119701, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25143064, Length=401, Percent_Identity=61.5960099750624, Blast_Score=535, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17533177, Length=330, Percent_Identity=33.030303030303, Blast_Score=163, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6319831, Length=402, Percent_Identity=59.7014925373134, Blast_Score=516, Evalue=1e-147,
Organism=Drosophila melanogaster, GI20129463, Length=400, Percent_Identity=64.75, Blast_Score=573, Evalue=1e-164,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45361; Mature: 45361

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQQLNNLTLPIYMDYQATTPLDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAERAVEEAR
CCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
SRVARLIGADTKEIIFTSGATESNNLAIKGVAKFYGNKKNHIITVVSEHKCVLDACRYLE
HHHHHHHCCCCHHEEEECCCCCCCCEEEEEHHHHHCCCCCEEEEEEECCHHHHHHHHHHH
QEGINITYLPVKPNGIIALETLKNAITDQTMLVSVMAVNNEIGVVQPLKEIGKICRARGV
HCCCEEEEEEECCCCEEEHHHHHHHHCCHHHEEEEEEECCCCCHHHHHHHHHHHHHHCCC
FFHSDIAQGFGKIPIDVNEFNIDLASISGHKIYGPKGIGGLYVRKKPRVRVTPLINGGGQ
EEECHHHHCCCCCCEEEEEEEEEEEECCCCEEECCCCCCEEEEECCCCEEEEEEECCCCC
ERGMRSGTLPTPLIVGLGVAAEIAYSEMEKDTKHVNYLFDRFLNNIHSRISEVYLNGAKD
CCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PRYKGNLNLSFAGVEGESIILAIKDLAVSSGSACTSASLEPSYVLRSMGIGEELAHTSIR
CCCCCCEEEEEECCCCCEEEEEEEEHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHH
FGIGRFTTEQEIDYAVNLICSKIDKLRKLSPLWEMMQEGIDLKKIKWAVH
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHEEEECC
>Mature Secondary Structure
MNQQLNNLTLPIYMDYQATTPLDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAERAVEEAR
CCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
SRVARLIGADTKEIIFTSGATESNNLAIKGVAKFYGNKKNHIITVVSEHKCVLDACRYLE
HHHHHHHCCCCHHEEEECCCCCCCCEEEEEHHHHHCCCCCEEEEEEECCHHHHHHHHHHH
QEGINITYLPVKPNGIIALETLKNAITDQTMLVSVMAVNNEIGVVQPLKEIGKICRARGV
HCCCEEEEEEECCCCEEEHHHHHHHHCCHHHEEEEEEECCCCCHHHHHHHHHHHHHHCCC
FFHSDIAQGFGKIPIDVNEFNIDLASISGHKIYGPKGIGGLYVRKKPRVRVTPLINGGGQ
EEECHHHHCCCCCCEEEEEEEEEEEECCCCEEECCCCCCEEEEECCCCEEEEEEECCCCC
ERGMRSGTLPTPLIVGLGVAAEIAYSEMEKDTKHVNYLFDRFLNNIHSRISEVYLNGAKD
CCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PRYKGNLNLSFAGVEGESIILAIKDLAVSSGSACTSASLEPSYVLRSMGIGEELAHTSIR
CCCCCCEEEEEECCCCCEEEEEEEEHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHH
FGIGRFTTEQEIDYAVNLICSKIDKLRKLSPLWEMMQEGIDLKKIKWAVH
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA