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The map label for this gene is rfaJ [H]

Identifier: 157825832

GI number: 157825832

Start: 690094

End: 691554

Strand: Reverse

Name: rfaJ [H]

Synonym: A1C_03845

Alternate gene names: 157825832

Gene position: 691554-690094 (Counterclockwise)

Preceding gene: 157825837

Following gene: 157825831

Centisome position: 56.18

GC content: 29.09

Gene sequence:

>1461_bases
ATGCATAAAAATACAAGTTGTATTATGGATATTGGGGCTTATAGTTATAAAAAGTTCTATAGTACAATACGTGAAGATCA
ATTAAATTTTGATCTAGCACTTGAACAATTAGAAGATAGTGAATCTTTAAACTCAAGCGATAAAGCAAGGTTAGAAAAAA
TTTGCTCTTTTCAAGTAGAAGACGGAATGATGCGTTTAGAGGTAGCCAAGGGTTATGATTGCTTACTACGTCTTTATCTA
GACAAAAATGCTACTTTAGTCCAGCAATATAAAGCGATTAAAAACGGTAGTTATGGTTTAAATACTTTATATACTAAAAA
AGCTGCGGTTAATTTAGCTAAACTCATTATTCTAGGTTACGAAGGGGAAGTAAAAGAAATATTAGAAATAAAAAATGATG
ATGATTTCCTAAAATATTATAGCGGTTATACAGTCGATGAGATTCATTTTTTAAATAGTTTAATCTCTCTTAATATACCG
GAACTTACTGCTAGATGTTTATATAGGCTTAGCTTAATTCATGTACTAGGCAGGTCATCTTATAATGAAAAAACTATACA
AACTGATTATAAGATTGCCATAGAAGAAATACAAAATGTAATTAAGTTAACTGGTATAAAGCAAAATAACATACTAGATA
TAGCATTAACTATTAATGATCAGTTTGCTATTCATGCCGGTGCAGTAATAGCCTCTAGTTTACTAAATAGTGATTTAGAT
AGTTTCTATAGATTTCATATTGTTATGGATTCTAATGACCCTGTTAGTCAAGAATCAATGGAAAAATTATCTTCCATGAA
ATATATCAGAGATTATTCTATAGACTTTACTACTTTCCCTGAAAATATATTAAATCAAGCTTTAACTGATAAGAAAATAA
AATTTTCGGATAATTGGCCTAGTTTAGTAATATATAGGTTATATTTTGATCAGATCTTTCCTCAGCTAGACTCTATATTA
TATCTTGATGCGGATGTTATAGTGTTGCATGATTTGAATTCTCTTAAAAAAATAGATATGAGTAATTATATTGCCGCAGG
TAGCATAGATACAGGTATTACATATTGTAATCATAAATTGATAAAAGAATGTAAAAGAAATATGGCTCATTCTTACAAAA
ATAGCGGGATAATATTTTTAAACTTAAAAAATATGCGAGAAAAACAAACTGAATCTGCGATATTAGAAACTATAAAAAAA
TCAGAATGTGACTTTTCGTTTCCTGATCAAGATGTGTTAAATATAGCTTTTCATGATTACATTTATCCTTTGTCAATGAG
ATGGAACTTTTTGACATATTTTAAGAATCAAAGCCCTTATTTTTCTTACTTCATTTTACACTATGCAGGACCTAAGCCAT
GGACTACCGATAAGCATGAACTATGGCAGACAAATCAGGACAAATTAGATAAAATGACAAAATACTACTGGCGATATAGA
GAAATAACACCTTGGAGTTAG

Upstream 100 bases:

>100_bases
TTTTTATAAATATATAATGTCTCGTACGAATTTAATATTATCCTTTTGTATTTTTCTAATGATAATAGTTATTGCTACTT
TTTTCATAAGTAATTACAGC

Downstream 100 bases:

>100_bases
TATAAATTCATTATTTTAATTGACTTTAGCACTTAAGAGATTTAAAAGTTAACTTTAATATGTAAGGTCACAATAAAAAT
GTTACCGCCTAAAATTTTCT

Product: lipopolysaccharide 1,2-glucosyltransferase RfaJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MHKNTSCIMDIGAYSYKKFYSTIREDQLNFDLALEQLEDSESLNSSDKARLEKICSFQVEDGMMRLEVAKGYDCLLRLYL
DKNATLVQQYKAIKNGSYGLNTLYTKKAAVNLAKLIILGYEGEVKEILEIKNDDDFLKYYSGYTVDEIHFLNSLISLNIP
ELTARCLYRLSLIHVLGRSSYNEKTIQTDYKIAIEEIQNVIKLTGIKQNNILDIALTINDQFAIHAGAVIASSLLNSDLD
SFYRFHIVMDSNDPVSQESMEKLSSMKYIRDYSIDFTTFPENILNQALTDKKIKFSDNWPSLVIYRLYFDQIFPQLDSIL
YLDADVIVLHDLNSLKKIDMSNYIAAGSIDTGITYCNHKLIKECKRNMAHSYKNSGIIFLNLKNMREKQTESAILETIKK
SECDFSFPDQDVLNIAFHDYIYPLSMRWNFLTYFKNQSPYFSYFILHYAGPKPWTTDKHELWQTNQDKLDKMTKYYWRYR
EITPWS

Sequences:

>Translated_486_residues
MHKNTSCIMDIGAYSYKKFYSTIREDQLNFDLALEQLEDSESLNSSDKARLEKICSFQVEDGMMRLEVAKGYDCLLRLYL
DKNATLVQQYKAIKNGSYGLNTLYTKKAAVNLAKLIILGYEGEVKEILEIKNDDDFLKYYSGYTVDEIHFLNSLISLNIP
ELTARCLYRLSLIHVLGRSSYNEKTIQTDYKIAIEEIQNVIKLTGIKQNNILDIALTINDQFAIHAGAVIASSLLNSDLD
SFYRFHIVMDSNDPVSQESMEKLSSMKYIRDYSIDFTTFPENILNQALTDKKIKFSDNWPSLVIYRLYFDQIFPQLDSIL
YLDADVIVLHDLNSLKKIDMSNYIAAGSIDTGITYCNHKLIKECKRNMAHSYKNSGIIFLNLKNMREKQTESAILETIKK
SECDFSFPDQDVLNIAFHDYIYPLSMRWNFLTYFKNQSPYFSYFILHYAGPKPWTTDKHELWQTNQDKLDKMTKYYWRYR
EITPWS
>Mature_486_residues
MHKNTSCIMDIGAYSYKKFYSTIREDQLNFDLALEQLEDSESLNSSDKARLEKICSFQVEDGMMRLEVAKGYDCLLRLYL
DKNATLVQQYKAIKNGSYGLNTLYTKKAAVNLAKLIILGYEGEVKEILEIKNDDDFLKYYSGYTVDEIHFLNSLISLNIP
ELTARCLYRLSLIHVLGRSSYNEKTIQTDYKIAIEEIQNVIKLTGIKQNNILDIALTINDQFAIHAGAVIASSLLNSDLD
SFYRFHIVMDSNDPVSQESMEKLSSMKYIRDYSIDFTTFPENILNQALTDKKIKFSDNWPSLVIYRLYFDQIFPQLDSIL
YLDADVIVLHDLNSLKKIDMSNYIAAGSIDTGITYCNHKLIKECKRNMAHSYKNSGIIFLNLKNMREKQTESAILETIKK
SECDFSFPDQDVLNIAFHDYIYPLSMRWNFLTYFKNQSPYFSYFILHYAGPKPWTTDKHELWQTNQDKLDKMTKYYWRYR
EITPWS

Specific function: Adds the glucose(II) group on the galactose(I) group of LPS [H]

COG id: COG1442

COG function: function code M; Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 8 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002495
- InterPro:   IPR013645 [H]

Pfam domain/function: PF01501 Glyco_transf_8; PF08437 Glyco_transf_8N [H]

EC number: =2.4.1.58 [H]

Molecular weight: Translated: 56687; Mature: 56687

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKNTSCIMDIGAYSYKKFYSTIREDQLNFDLALEQLEDSESLNSSDKARLEKICSFQVE
CCCCCCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
DGMMRLEVAKGYDCLLRLYLDKNATLVQQYKAIKNGSYGLNTLYTKKAAVNLAKLIILGY
CCEEEEEECCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCEEEEEEECC
EGEVKEILEIKNDDDFLKYYSGYTVDEIHFLNSLISLNIPELTARCLYRLSLIHVLGRSS
CCCHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
YNEKTIQTDYKIAIEEIQNVIKLTGIKQNNILDIALTINDQFAIHAGAVIASSLLNSDLD
CCCCEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEHHHHHHHHHHHHHCHH
SFYRFHIVMDSNDPVSQESMEKLSSMKYIRDYSIDFTTFPENILNQALTDKKIKFSDNWP
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCCCEEECCCCC
SLVIYRLYFDQIFPQLDSILYLDADVIVLHDLNSLKKIDMSNYIAAGSIDTGITYCNHKL
HHHHHHHHHHHHHHHHHCEEEECCCEEEEECCCHHHHCCCCCEEECCCCCCCHHHHHHHH
IKECKRNMAHSYKNSGIIFLNLKNMREKQTESAILETIKKSECDFSFPDQDVLNIAFHDY
HHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
IYPLSMRWNFLTYFKNQSPYFSYFILHYAGPKPWTTDKHELWQTNQDKLDKMTKYYWRYR
EEEEEECEEEEEEECCCCCCEEEEEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHC
EITPWS
CCCCCC
>Mature Secondary Structure
MHKNTSCIMDIGAYSYKKFYSTIREDQLNFDLALEQLEDSESLNSSDKARLEKICSFQVE
CCCCCCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
DGMMRLEVAKGYDCLLRLYLDKNATLVQQYKAIKNGSYGLNTLYTKKAAVNLAKLIILGY
CCEEEEEECCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCEEEEEEECC
EGEVKEILEIKNDDDFLKYYSGYTVDEIHFLNSLISLNIPELTARCLYRLSLIHVLGRSS
CCCHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
YNEKTIQTDYKIAIEEIQNVIKLTGIKQNNILDIALTINDQFAIHAGAVIASSLLNSDLD
CCCCEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEHHHHHHHHHHHHHCHH
SFYRFHIVMDSNDPVSQESMEKLSSMKYIRDYSIDFTTFPENILNQALTDKKIKFSDNWP
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCCCEEECCCCC
SLVIYRLYFDQIFPQLDSILYLDADVIVLHDLNSLKKIDMSNYIAAGSIDTGITYCNHKL
HHHHHHHHHHHHHHHHHCEEEECCCEEEEECCCHHHHCCCCCEEECCCCCCCHHHHHHHH
IKECKRNMAHSYKNSGIIFLNLKNMREKQTESAILETIKKSECDFSFPDQDVLNIAFHDY
HHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
IYPLSMRWNFLTYFKNQSPYFSYFILHYAGPKPWTTDKHELWQTNQDKLDKMTKYYWRYR
EEEEEECEEEEEEECCCCCCEEEEEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHC
EITPWS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2235496; 9535865; 11677609 [H]