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The map label for this gene is miaA

Identifier: 157825775

GI number: 157825775

Start: 635172

End: 636110

Strand: Reverse

Name: miaA

Synonym: A1C_03530

Alternate gene names: 157825775

Gene position: 636110-635172 (Counterclockwise)

Preceding gene: 157825776

Following gene: 157825767

Centisome position: 51.67

GC content: 31.95

Gene sequence:

>939_bases
GTGACAACGAAAGACATAATAATTTTATGCGGGCCAACTGCTAGTGGAAAGTCTTATTTAGGGCATGAACTAGCTAAAGC
ATATAACGGCGAGATCATAAATATCGACTCAATGCAGGTTTATAAGAAAATTCCTATTATTACCGCCTCCCCTCCGCAAA
GCTATACAGCTGAAGTGACTTACCATTTATATAATTTTCTATCTATTACGGAAGATTTTTCGGTAGTAAAATATCTCAAA
CTTGCTGCAGAAAAAATAAAAGAAGTAACTGCTCGAGTCAAACTACCTATATTAATAGGTGGAACTGGTTTATATATCAA
TTCTTTAGTTTTCGGATATAATAATATTCCTGATATATCAGAAGATTTACGAGAACAAGTAAGAAACTTACATACTGAAA
TAGGTAATATAGAATTATGGAATAAATTAGAGGAGCTTGACACGCTTGCTGCTTCTAAAATAAACAAAAATGATACTCAG
CGTTTAATTAGAGCTTATGAAGTATTCTTGGAAACTGGTAAATCTATTTGTTCCTTTCAAACTTTACCAAAAGAGCAAAT
TTTATCTGATTTTAATTTCAAAATTATCTTCTTAAATCCTGAGCGAAAATTTTTATATAAAACTTGTGATGAGCGTTTAG
ATAAGATATTTAAAGAAGGGGCAATTGACGAAATAGCTTTAATAAAAAAGCAATTTGCTCCTAAAGATTATGTGGATTTA
AAAGCTGTAGGAATAAAAGAAATTTTAGCTTATTTAGACGGCGATATGACTTTAGATAGAGCTCTCAATGCAGCTCAAAT
ACGAACCCGTCAGTATGCTAAAAGACAAGTTACTTGGTTTAAAAATCAGATACAAGATAAAACCACATTGGAATATTCTA
ACCAAGAAGAGTTTGCACAAATATTAAATGTCATTCCTGCGGAGGCGGGAATTCACTAA

Upstream 100 bases:

>100_bases
TTTTACTGGATTGCTTCGTCAATGACTCTGTAATTTCTTCGCAATGACGAAAAACCGAGCCATGCAACGACCCAACAATT
ATAGACTAAACTATATATCA

Downstream 100 bases:

>100_bases
AATAAAGTTCCTTAAAGCTTTATTTGAAAAAAAATTATCTAATAAAAAATCTTTAATTTTAAAGATTTTTTAGCATGGAT
TCCTACTTTCGCAATGGATG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTTKDIIILCGPTASGKSYLGHELAKAYNGEIINIDSMQVYKKIPIITASPPQSYTAEVTYHLYNFLSITEDFSVVKYLK
LAAEKIKEVTARVKLPILIGGTGLYINSLVFGYNNIPDISEDLREQVRNLHTEIGNIELWNKLEELDTLAASKINKNDTQ
RLIRAYEVFLETGKSICSFQTLPKEQILSDFNFKIIFLNPERKFLYKTCDERLDKIFKEGAIDEIALIKKQFAPKDYVDL
KAVGIKEILAYLDGDMTLDRALNAAQIRTRQYAKRQVTWFKNQIQDKTTLEYSNQEEFAQILNVIPAEAGIH

Sequences:

>Translated_312_residues
MTTKDIIILCGPTASGKSYLGHELAKAYNGEIINIDSMQVYKKIPIITASPPQSYTAEVTYHLYNFLSITEDFSVVKYLK
LAAEKIKEVTARVKLPILIGGTGLYINSLVFGYNNIPDISEDLREQVRNLHTEIGNIELWNKLEELDTLAASKINKNDTQ
RLIRAYEVFLETGKSICSFQTLPKEQILSDFNFKIIFLNPERKFLYKTCDERLDKIFKEGAIDEIALIKKQFAPKDYVDL
KAVGIKEILAYLDGDMTLDRALNAAQIRTRQYAKRQVTWFKNQIQDKTTLEYSNQEEFAQILNVIPAEAGIH
>Mature_311_residues
TTKDIIILCGPTASGKSYLGHELAKAYNGEIINIDSMQVYKKIPIITASPPQSYTAEVTYHLYNFLSITEDFSVVKYLKL
AAEKIKEVTARVKLPILIGGTGLYINSLVFGYNNIPDISEDLREQVRNLHTEIGNIELWNKLEELDTLAASKINKNDTQR
LIRAYEVFLETGKSICSFQTLPKEQILSDFNFKIIFLNPERKFLYKTCDERLDKIFKEGAIDEIALIKKQFAPKDYVDLK
AVGIKEILAYLDGDMTLDRALNAAQIRTRQYAKRQVTWFKNQIQDKTTLEYSNQEEFAQILNVIPAEAGIH

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=314, Percent_Identity=27.0700636942675, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1790613, Length=278, Percent_Identity=30.2158273381295, Blast_Score=148, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI25144712, Length=305, Percent_Identity=28.8524590163934, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324848, Length=298, Percent_Identity=30.8724832214765, Blast_Score=145, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24649830, Length=334, Percent_Identity=24.251497005988, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_RICAH (A8GNL2)

Other databases:

- EMBL:   CP000847
- RefSeq:   YP_001493495.1
- ProteinModelPortal:   A8GNL2
- SMR:   A8GNL2
- STRING:   A8GNL2
- GeneID:   5644614
- GenomeReviews:   CP000847_GR
- KEGG:   rak:A1C_03530
- NMPDR:   fig|293614.3.peg.659
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   RAKA293614:A1C_03530-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35615; Mature: 35484

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKDIIILCGPTASGKSYLGHELAKAYNGEIINIDSMQVYKKIPIITASPPQSYTAEVT
CCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEECHHHHHHHCCEEECCCCCCHHHHHH
YHLYNFLSITEDFSVVKYLKLAAEKIKEVTARVKLPILIGGTGLYINSLVFGYNNIPDIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHCCCCCCCHH
EDLREQVRNLHTEIGNIELWNKLEELDTLAASKINKNDTQRLIRAYEVFLETGKSICSFQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHH
TLPKEQILSDFNFKIIFLNPERKFLYKTCDERLDKIFKEGAIDEIALIKKQFAPKDYVDL
CCCHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHH
KAVGIKEILAYLDGDMTLDRALNAAQIRTRQYAKRQVTWFKNQIQDKTTLEYSNQEEFAQ
HHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHH
ILNVIPAEAGIH
HHHHCCCCCCCC
>Mature Secondary Structure 
TTKDIIILCGPTASGKSYLGHELAKAYNGEIINIDSMQVYKKIPIITASPPQSYTAEVT
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEECHHHHHHHCCEEECCCCCCHHHHHH
YHLYNFLSITEDFSVVKYLKLAAEKIKEVTARVKLPILIGGTGLYINSLVFGYNNIPDIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHCCCCCCCHH
EDLREQVRNLHTEIGNIELWNKLEELDTLAASKINKNDTQRLIRAYEVFLETGKSICSFQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHH
TLPKEQILSDFNFKIIFLNPERKFLYKTCDERLDKIFKEGAIDEIALIKKQFAPKDYVDL
CCCHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHH
KAVGIKEILAYLDGDMTLDRALNAAQIRTRQYAKRQVTWFKNQIQDKTTLEYSNQEEFAQ
HHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHH
ILNVIPAEAGIH
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA