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The map label for this gene is rne [H]

Identifier: 157825471

GI number: 157825471

Start: 346962

End: 349034

Strand: Direct

Name: rne [H]

Synonym: A1C_01860

Alternate gene names: 157825471

Gene position: 346962-349034 (Clockwise)

Preceding gene: 157825468

Following gene: 157825472

Centisome position: 28.18

GC content: 32.27

Gene sequence:

>2073_bases
ATGAATAAAAAGATTATAATTGATGCTAATTTTCCAAGTGAAACAAGAGTAGTTTTATTAGGACAAAGCAACAATATAGA
AGATATTGAATTTCAAACAACCGTAAGACAACAAAACAAAGGTAACATTTATTTAGCAAAAGTTACAAGAATAGAGCCAT
CGTTACAAGCTGTTTTTATAGAGTATGGAATGGAAAAAAGCGGTTTCTTACCGTTTAGTGAAATCCATCCTAACTATTAC
AATTTGCCTACCTCGGAGAGAAATTTTCCGGTTAATGCTTTTCCTGAAATAGCTCTTTCAAATATTACGGTTGAGGATGA
TAAAGAGAAACCGGCTGTTATATATGATTCTTTCGTTGATAGTGAAGAAATTGACCTAAAAACTATAGAAGATTTAGTAG
AGAGTAAATTCCAGTCAGAACTTAATTTAGAAGCGGCTGATGATATTGAAATTATTCAAAGCGGTACCACTGAGTCAAAT
ATTCCGCAATATAAACAATATAAAGTGCAAGAAGTAATCAGAAAAACCCAAATATTATTAGTACAGGTTACTAAGGAAGA
ACGAGGAAATAAGTGTGCCGCTTTTACTACGTATATATCTTTAGCCGGCAAATATTGTGTTTTAATGCCTAATAAAGGTT
TGCAAAACGGCATATCACGTAAAATATCAAACGGGGAAGAAAGAAAAAGACTGAAAGATATTTTAAATAAAATAGTAAGT
GGTGACAAAAATACTTATAGTGTGATTGTTAGAACTGCAGGTAGAGGAAGTAGTACCTTAGATTTGAAGAAAGACTATAA
TTATTTAGCAAGGTTATGGAATAAAATTCGTAAAAGTACTATTAAATTTCCAGCTCCGTGTTTTATCCATGAAGAAGATA
GTATAATACGTAAAACCATACGTGATATGTGTGATCACAATGTTAAAGAAGTAGTGATTCAAGGACAAGAAGCCTATGAA
GATGCTTCAAGATTTATGCAGGATTTATTACCTTCAGAGCTTGCAAAGCTGAAAGAGCATAAAAATAAAATTCCTATATT
TACTAAGTTTCAAGTAGAAGAGCAATTAGTTAAATTGTATCAGCCTGTCGTAACACTACCTTCAGGGGGATATATCGTTA
TTAATCCTACGGAAGCTCTTATTTCAATTGATGTAAATTCCGGTAAATCAACTTCTGAAAAAAATATCGAAGAAACGGCA
CTAAAAACTAATTTAGAAGCAGCAAAAGAAGTAGCAAAGCAAGTAAAGCTTAGAGATCTATCAGGTTTGATAGTTGTTGA
TTTTATAGATATGAGCGAAGCTAAGAATCGTAAAATTATTGAACGATCCTTTAAAGAGTTTTTAAGTCGTGATCGTGCTC
GTATACAAACCGGTAATATTAGTCAGTTTGGGCTCCTTGAATTCTCAAGGCAGCGATTACGTTCTTCTTTCTTAGAAACT
AATTCGGCTATTTGTTCTCATTGTAACGGTAAAGGAGTCATTAGGGCGAGTGATGCTAATGCTATGTTAATTTTACGTAC
TATAGAGAATGAAATTTTTGAAGAAAGAATTGATGTAATAAATGTTTTTACCAATATCGTTTCAGTTCTTTATCTACTTA
ATAATAAACGTGCTGAGATAAAATTTATTGAAGAAAAATATAATATAAAGCTTAATTTTTATTCCGATCCTAATGCTACA
TCAGATAGTTATTCAATTGAAAAAGTAAAGCTGTTGAAGCAAAACAACAATAATGTTAATCCTGTTAAGCCGGTCATTCA
GAATCATAGTGCTGATTATACTGAAGAAGAGCCGAAAACAGAACAGTTGCGCAAAAATAAACATAAATGGAAGACAGCTA
ATAATAGTAATAATGTTGCTAATGAAGTAAAAGATAAGAGTTTAGAGGCAGCGGAATCGGTAGAGCACACTGTAAGTGTT
GTGGAAGAGCTGAAAGTTGTAGAAAGTAATGCTGGTTTAGAAGAAGCACAAGTTGCTCCAAAAAAGATAAAACGTAGATA
TCGTAATAAAAAATCAAATAAAACATGTCCTCCTAATATCGCCGAAGATACGGAACAGAATGTAGAGTCTTGA

Upstream 100 bases:

>100_bases
ATTATCTATTTAAATAAATTAATACAAGATAAACAATGATTGTTCTTAAGATACAGGCATTATATTAATTATTTGTTCTT
CAAGTTAGTGAGCAAGATAA

Downstream 100 bases:

>100_bases
TTAGGAAGGGCTGCTTCGTCAAAACTTATAGTTTTTCCTCGCAATGACGACTTGCAGTATCCATGCGACAAGATCTTTGC
AGGAATGACAAGATAAAAAA

Product: ribonuclease E

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 690; Mature: 690

Protein sequence:

>690_residues
MNKKIIIDANFPSETRVVLLGQSNNIEDIEFQTTVRQQNKGNIYLAKVTRIEPSLQAVFIEYGMEKSGFLPFSEIHPNYY
NLPTSERNFPVNAFPEIALSNITVEDDKEKPAVIYDSFVDSEEIDLKTIEDLVESKFQSELNLEAADDIEIIQSGTTESN
IPQYKQYKVQEVIRKTQILLVQVTKEERGNKCAAFTTYISLAGKYCVLMPNKGLQNGISRKISNGEERKRLKDILNKIVS
GDKNTYSVIVRTAGRGSSTLDLKKDYNYLARLWNKIRKSTIKFPAPCFIHEEDSIIRKTIRDMCDHNVKEVVIQGQEAYE
DASRFMQDLLPSELAKLKEHKNKIPIFTKFQVEEQLVKLYQPVVTLPSGGYIVINPTEALISIDVNSGKSTSEKNIEETA
LKTNLEAAKEVAKQVKLRDLSGLIVVDFIDMSEAKNRKIIERSFKEFLSRDRARIQTGNISQFGLLEFSRQRLRSSFLET
NSAICSHCNGKGVIRASDANAMLILRTIENEIFEERIDVINVFTNIVSVLYLLNNKRAEIKFIEEKYNIKLNFYSDPNAT
SDSYSIEKVKLLKQNNNNVNPVKPVIQNHSADYTEEEPKTEQLRKNKHKWKTANNSNNVANEVKDKSLEAAESVEHTVSV
VEELKVVESNAGLEEAQVAPKKIKRRYRNKKSNKTCPPNIAEDTEQNVES

Sequences:

>Translated_690_residues
MNKKIIIDANFPSETRVVLLGQSNNIEDIEFQTTVRQQNKGNIYLAKVTRIEPSLQAVFIEYGMEKSGFLPFSEIHPNYY
NLPTSERNFPVNAFPEIALSNITVEDDKEKPAVIYDSFVDSEEIDLKTIEDLVESKFQSELNLEAADDIEIIQSGTTESN
IPQYKQYKVQEVIRKTQILLVQVTKEERGNKCAAFTTYISLAGKYCVLMPNKGLQNGISRKISNGEERKRLKDILNKIVS
GDKNTYSVIVRTAGRGSSTLDLKKDYNYLARLWNKIRKSTIKFPAPCFIHEEDSIIRKTIRDMCDHNVKEVVIQGQEAYE
DASRFMQDLLPSELAKLKEHKNKIPIFTKFQVEEQLVKLYQPVVTLPSGGYIVINPTEALISIDVNSGKSTSEKNIEETA
LKTNLEAAKEVAKQVKLRDLSGLIVVDFIDMSEAKNRKIIERSFKEFLSRDRARIQTGNISQFGLLEFSRQRLRSSFLET
NSAICSHCNGKGVIRASDANAMLILRTIENEIFEERIDVINVFTNIVSVLYLLNNKRAEIKFIEEKYNIKLNFYSDPNAT
SDSYSIEKVKLLKQNNNNVNPVKPVIQNHSADYTEEEPKTEQLRKNKHKWKTANNSNNVANEVKDKSLEAAESVEHTVSV
VEELKVVESNAGLEEAQVAPKKIKRRYRNKKSNKTCPPNIAEDTEQNVES
>Mature_690_residues
MNKKIIIDANFPSETRVVLLGQSNNIEDIEFQTTVRQQNKGNIYLAKVTRIEPSLQAVFIEYGMEKSGFLPFSEIHPNYY
NLPTSERNFPVNAFPEIALSNITVEDDKEKPAVIYDSFVDSEEIDLKTIEDLVESKFQSELNLEAADDIEIIQSGTTESN
IPQYKQYKVQEVIRKTQILLVQVTKEERGNKCAAFTTYISLAGKYCVLMPNKGLQNGISRKISNGEERKRLKDILNKIVS
GDKNTYSVIVRTAGRGSSTLDLKKDYNYLARLWNKIRKSTIKFPAPCFIHEEDSIIRKTIRDMCDHNVKEVVIQGQEAYE
DASRFMQDLLPSELAKLKEHKNKIPIFTKFQVEEQLVKLYQPVVTLPSGGYIVINPTEALISIDVNSGKSTSEKNIEETA
LKTNLEAAKEVAKQVKLRDLSGLIVVDFIDMSEAKNRKIIERSFKEFLSRDRARIQTGNISQFGLLEFSRQRLRSSFLET
NSAICSHCNGKGVIRASDANAMLILRTIENEIFEERIDVINVFTNIVSVLYLLNNKRAEIKFIEEKYNIKLNFYSDPNAT
SDSYSIEKVKLLKQNNNNVNPVKPVIQNHSADYTEEEPKTEQLRKNKHKWKTANNSNNVANEVKDKSLEAAESVEHTVSV
VEELKVVESNAGLEEAQVAPKKIKRRYRNKKSNKTCPPNIAEDTEQNVES

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=391, Percent_Identity=41.4322250639386, Blast_Score=317, Evalue=2e-87,
Organism=Escherichia coli, GI87082235, Length=346, Percent_Identity=31.2138728323699, Blast_Score=189, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 78600; Mature: 78600

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKIIIDANFPSETRVVLLGQSNNIEDIEFQTTVRQQNKGNIYLAKVTRIEPSLQAVFI
CCCEEEEECCCCCCCEEEEEECCCCCCCEEEHHHHHHCCCCCEEEEEEEECCCHHHHHHH
EYGMEKSGFLPFSEIHPNYYNLPTSERNFPVNAFPEIALSNITVEDDKEKPAVIYDSFVD
HHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCEEEECCCCCCEEEEECCCC
SEEIDLKTIEDLVESKFQSELNLEAADDIEIIQSGTTESNIPQYKQYKVQEVIRKTQILL
CCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHEEE
VQVTKEERGNKCAAFTTYISLAGKYCVLMPNKGLQNGISRKISNGEERKRLKDILNKIVS
EEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHC
GDKNTYSVIVRTAGRGSSTLDLKKDYNYLARLWNKIRKSTIKFPAPCFIHEEDSIIRKTI
CCCCEEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHH
RDMCDHNVKEVVIQGQEAYEDASRFMQDLLPSELAKLKEHKNKIPIFTKFQVEEQLVKLY
HHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
QPVVTLPSGGYIVINPTEALISIDVNSGKSTSEKNIEETALKTNLEAAKEVAKQVKLRDL
HHHEECCCCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
SGLIVVDFIDMSEAKNRKIIERSFKEFLSRDRARIQTGNISQFGLLEFSRQRLRSSFLET
CCEEEEEEECCCCCCCHHHHHHHHHHHHHCCHHEEECCCCCHHHHHHHHHHHHHHHHHHC
NSAICSHCNGKGVIRASDANAMLILRTIENEIFEERIDVINVFTNIVSVLYLLNNKRAEI
CHHHHHCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
KFIEEKYNIKLNFYSDPNATSDSYSIEKVKLLKQNNNNVNPVKPVIQNHSADYTEEEPKT
EEEEEEEEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCH
EQLRKNKHKWKTANNSNNVANEVKDKSLEAAESVEHTVSVVEELKVVESNAGLEEAQVAP
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCH
KKIKRRYRNKKSNKTCPPNIAEDTEQNVES
HHHHHHHHCCCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MNKKIIIDANFPSETRVVLLGQSNNIEDIEFQTTVRQQNKGNIYLAKVTRIEPSLQAVFI
CCCEEEEECCCCCCCEEEEEECCCCCCCEEEHHHHHHCCCCCEEEEEEEECCCHHHHHHH
EYGMEKSGFLPFSEIHPNYYNLPTSERNFPVNAFPEIALSNITVEDDKEKPAVIYDSFVD
HHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCEEEECCCCCCEEEEECCCC
SEEIDLKTIEDLVESKFQSELNLEAADDIEIIQSGTTESNIPQYKQYKVQEVIRKTQILL
CCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHEEE
VQVTKEERGNKCAAFTTYISLAGKYCVLMPNKGLQNGISRKISNGEERKRLKDILNKIVS
EEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHC
GDKNTYSVIVRTAGRGSSTLDLKKDYNYLARLWNKIRKSTIKFPAPCFIHEEDSIIRKTI
CCCCEEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHH
RDMCDHNVKEVVIQGQEAYEDASRFMQDLLPSELAKLKEHKNKIPIFTKFQVEEQLVKLY
HHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
QPVVTLPSGGYIVINPTEALISIDVNSGKSTSEKNIEETALKTNLEAAKEVAKQVKLRDL
HHHEECCCCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
SGLIVVDFIDMSEAKNRKIIERSFKEFLSRDRARIQTGNISQFGLLEFSRQRLRSSFLET
CCEEEEEEECCCCCCCHHHHHHHHHHHHHCCHHEEECCCCCHHHHHHHHHHHHHHHHHHC
NSAICSHCNGKGVIRASDANAMLILRTIENEIFEERIDVINVFTNIVSVLYLLNNKRAEI
CHHHHHCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
KFIEEKYNIKLNFYSDPNATSDSYSIEKVKLLKQNNNNVNPVKPVIQNHSADYTEEEPKT
EEEEEEEEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCH
EQLRKNKHKWKTANNSNNVANEVKDKSLEAAESVEHTVSVVEELKVVESNAGLEEAQVAP
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCH
KKIKRRYRNKKSNKTCPPNIAEDTEQNVES
HHHHHHHHCCCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1447789; 1704367; 2011493; 8415644 [H]