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The map label for this gene is lasT [H]

Identifier: 157825295

GI number: 157825295

Start: 165571

End: 166266

Strand: Direct

Name: lasT [H]

Synonym: A1C_00885

Alternate gene names: 157825295

Gene position: 165571-166266 (Clockwise)

Preceding gene: 157825294

Following gene: 157825299

Centisome position: 13.45

GC content: 35.34

Gene sequence:

>696_bases
ATGAATCCGGTTATAATATTAGTTGTCCCGCAAATGGGTGAGAATATCGGTGCTACTGCAAGAGCCATGAAGAATTTTGG
CTTAAGTGAGCTGAGAATCGTAGCTCCAAGAGATGGCTGGCCAAATGAGCAAGCACGTAGTAATGCAGTCGGAGCGGTTA
ATATTATAGATAATGCAAAAATTTATGATAACCTAGAGGAGAGCGTTCAAGACCTTGAATATTTATACGCTACTACTTGT
ATTAAAAGGGCGATGAATAAAGATTACGTATTTTCACAAAATTTGCCTTTAGATTATCCAAATTCTGAAAAAGTTGGGAT
AATGTTTGGGCGTGAAAATAATGGTCTTAGCAATAAAGAAGTCGCACTCGCTAATAAGATTATCACTATTAACACAACTG
AATTTAGCTCGTTAAATATCGCTCAAGCGGTTATTATTATCTGCTATGAATTGTTCCGTAATTTTAAGCCTAGAGAGGAT
ATACATAATATTCAAAAATTAGCTACTAAAGAAGAAATAGAACATTTTTTAACAAATTTATTCGGCAAACTTGATAAAGC
CAGATTCTTCAAAGCTCCTGACAAAAAGCCTGCCATGCAGCAAAATATCACTAATATATTTACACGTATCAATAGCCTCT
CCTCACCTGAAGTGCAAACACTGCAAGGTATCATAAAGTCTTTGTATACTCGATGA

Upstream 100 bases:

>100_bases
ATATGATAAAGCTTTTGCTGCCCGGCACTTCCTTAATTACTAATTCCGATGAAGCTGATGCTATAGCAATCGCTTATACT
TGTCATGTTATGAGAGTTAA

Downstream 100 bases:

>100_bases
ACTTCAAAAATTAGCGTGTCGTAGTTCTACAAAAGCTATGGTACTCACGTATTAAGTATACGCTGCGTTCCTCGCCTAGT
GAACTCCTCGCTCTTTTTGA

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MNPVIILVVPQMGENIGATARAMKNFGLSELRIVAPRDGWPNEQARSNAVGAVNIIDNAKIYDNLEESVQDLEYLYATTC
IKRAMNKDYVFSQNLPLDYPNSEKVGIMFGRENNGLSNKEVALANKIITINTTEFSSLNIAQAVIIICYELFRNFKPRED
IHNIQKLATKEEIEHFLTNLFGKLDKARFFKAPDKKPAMQQNITNIFTRINSLSSPEVQTLQGIIKSLYTR

Sequences:

>Translated_231_residues
MNPVIILVVPQMGENIGATARAMKNFGLSELRIVAPRDGWPNEQARSNAVGAVNIIDNAKIYDNLEESVQDLEYLYATTC
IKRAMNKDYVFSQNLPLDYPNSEKVGIMFGRENNGLSNKEVALANKIITINTTEFSSLNIAQAVIIICYELFRNFKPRED
IHNIQKLATKEEIEHFLTNLFGKLDKARFFKAPDKKPAMQQNITNIFTRINSLSSPEVQTLQGIIKSLYTR
>Mature_231_residues
MNPVIILVVPQMGENIGATARAMKNFGLSELRIVAPRDGWPNEQARSNAVGAVNIIDNAKIYDNLEESVQDLEYLYATTC
IKRAMNKDYVFSQNLPLDYPNSEKVGIMFGRENNGLSNKEVALANKIITINTTEFSSLNIAQAVIIICYELFRNFKPRED
IHNIQKLATKEEIEHFLTNLFGKLDKARFFKAPDKKPAMQQNITNIFTRINSLSSPEVQTLQGIIKSLYTR

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790865, Length=191, Percent_Identity=37.696335078534, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1788881, Length=232, Percent_Identity=29.3103448275862, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.-

Molecular weight: Translated: 26144; Mature: 26144

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPVIILVVPQMGENIGATARAMKNFGLSELRIVAPRDGWPNEQARSNAVGAVNIIDNAK
CCCEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCEEEEECCCCH
IYDNLEESVQDLEYLYATTCIKRAMNKDYVFSQNLPLDYPNSEKVGIMFGRENNGLSNKE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCCCC
VALANKIITINTTEFSSLNIAQAVIIICYELFRNFKPREDIHNIQKLATKEEIEHFLTNL
EEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
FGKLDKARFFKAPDKKPAMQQNITNIFTRINSLSSPEVQTLQGIIKSLYTR
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNPVIILVVPQMGENIGATARAMKNFGLSELRIVAPRDGWPNEQARSNAVGAVNIIDNAK
CCCEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCEEEEECCCCH
IYDNLEESVQDLEYLYATTCIKRAMNKDYVFSQNLPLDYPNSEKVGIMFGRENNGLSNKE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCCCC
VALANKIITINTTEFSSLNIAQAVIIICYELFRNFKPREDIHNIQKLATKEEIEHFLTNL
EEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
FGKLDKARFFKAPDKKPAMQQNITNIFTRINSLSSPEVQTLQGIIKSLYTR
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8022271 [H]