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The map label for this gene is gidB [H]

Identifier: 157825213

GI number: 157825213

Start: 74402

End: 74977

Strand: Direct

Name: gidB [H]

Synonym: A1C_00460

Alternate gene names: 157825213

Gene position: 74402-74977 (Clockwise)

Preceding gene: 157825212

Following gene: 157825214

Centisome position: 6.04

GC content: 25.0

Gene sequence:

>576_bases
ATGGAAGTTTCACGTGAAATAATTGAAAAATTAGAAATTTTTCAAAAGTTAGTTAAAAAATGGAATAAATCAATCAATCT
TGTATCGGGTAATACTATACATAATTTTTGGCAACGTCATATATTAGACTCATTACAATTAATGCAATATATTGATAATA
AAGAAATACACTTGGTTGATATAGGAAGTGGGGCCGGTTTTCCCGGTATAGTATTATCTATAGCAGGGGTAGCAAAAGTA
AGTTTGATAGAAGCTGATTTACGCAAATGTATATTTTTAGAGAAAGCATCAAAGATATCAAATAATAACATACAAATAAT
AAATCAAAGAATAGAAAAAGTAGCAATAGATTGTAGTATATTGACTTGTAGGGCTTTTTCTAAATTAAATACAATATTTA
ATTGTATAAAAAATATTTCAGTACAAGAAAAAGTTTTATTATTAAAAGGAAAAAATTATTTAACAGAAATAGTAGCAGCT
AAAGAGATGTGGTTATTTGATTATTTAATACATCAAAGTATTACTTGTGAAGAAGGGAAAATTCTAGAAGTTAGTAATTT
AACAAAAATTATATGA

Upstream 100 bases:

>100_bases
TCCCATAAACCTACTACTATTGGTTTAGCACGTCGAATTCCAGGGATTACACCTGCAGCAATTACGTCTATTATAATTTA
TTTAAAAACTAAATATAGTG

Downstream 100 bases:

>100_bases
AAGTAATTGCGATTGTTAATCAAAAAGGAGGAGTGGCTAAAACTACAACTACTGTAAATTTAGCTACAGCTTTTGCTGCG
GTAAATAAAAAAATTTTAGT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MEVSREIIEKLEIFQKLVKKWNKSINLVSGNTIHNFWQRHILDSLQLMQYIDNKEIHLVDIGSGAGFPGIVLSIAGVAKV
SLIEADLRKCIFLEKASKISNNNIQIINQRIEKVAIDCSILTCRAFSKLNTIFNCIKNISVQEKVLLLKGKNYLTEIVAA
KEMWLFDYLIHQSITCEEGKILEVSNLTKII

Sequences:

>Translated_191_residues
MEVSREIIEKLEIFQKLVKKWNKSINLVSGNTIHNFWQRHILDSLQLMQYIDNKEIHLVDIGSGAGFPGIVLSIAGVAKV
SLIEADLRKCIFLEKASKISNNNIQIINQRIEKVAIDCSILTCRAFSKLNTIFNCIKNISVQEKVLLLKGKNYLTEIVAA
KEMWLFDYLIHQSITCEEGKILEVSNLTKII
>Mature_191_residues
MEVSREIIEKLEIFQKLVKKWNKSINLVSGNTIHNFWQRHILDSLQLMQYIDNKEIHLVDIGSGAGFPGIVLSIAGVAKV
SLIEADLRKCIFLEKASKISNNNIQIINQRIEKVAIDCSILTCRAFSKLNTIFNCIKNISVQEKVLLLKGKNYLTEIVAA
KEMWLFDYLIHQSITCEEGKILEVSNLTKII

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=169, Percent_Identity=27.2189349112426, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 21758; Mature: 21758

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVSREIIEKLEIFQKLVKKWNKSINLVSGNTIHNFWQRHILDSLQLMQYIDNKEIHLVD
CCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IGSGAGFPGIVLSIAGVAKVSLIEADLRKCIFLEKASKISNNNIQIINQRIEKVAIDCSI
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LTCRAFSKLNTIFNCIKNISVQEKVLLLKGKNYLTEIVAAKEMWLFDYLIHQSITCEEGK
HHHHHHHHHHHHHHHHHCCCHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ILEVSNLTKII
EEEHHHHHHCC
>Mature Secondary Structure
MEVSREIIEKLEIFQKLVKKWNKSINLVSGNTIHNFWQRHILDSLQLMQYIDNKEIHLVD
CCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IGSGAGFPGIVLSIAGVAKVSLIEADLRKCIFLEKASKISNNNIQIINQRIEKVAIDCSI
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LTCRAFSKLNTIFNCIKNISVQEKVLLLKGKNYLTEIVAAKEMWLFDYLIHQSITCEEGK
HHHHHHHHHHHHHHHHHCCCHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ILEVSNLTKII
EEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA