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The map label for this gene is dut

Identifier: 157373076

GI number: 157373076

Start: 5363087

End: 5363545

Strand: Direct

Name: dut

Synonym: Spro_4844

Alternate gene names: 157373076

Gene position: 5363087-5363545 (Clockwise)

Preceding gene: 157373075

Following gene: 157373077

Centisome position: 98.43

GC content: 56.21

Gene sequence:

>459_bases
ATGATGAAAAAAATCGACGTTAAAATCCTGGACCCGCGTATTGGCAAGGATTTCCCATTACCGACCTACGCCACCCCTGG
CTCTGCAGGCCTGGATCTGCGCGCCTGCCTGGACAGCGCCGTTGAACTGGCACCGGGTGAAACCCAGCTGCTGCCTACCG
GCCTGGCTATTCACATCGCCGATACCGATCTTGCGGCAGTGATCTTGCCACGTTCCGGTCTGGGCCATAAACACGGCGTA
GTGCTGGGCAACCTGGTCGGCCTGATCGATTCCGATTATCAGGGTCAACTGATGGTTTCCGTCTGGAACCGCGGTCAAAA
ATCCTTCACTATTGAACCCGGCGAACGTATCGCGCAGATGGTATTTGTGCCGGTGGTACAAGCTGAGTTCAATCTGGTTG
AAGAATTCGACAGCAGCGAACGTGGTGCCGGTGGTTTTGGCCACTCCGGCCGCCACTAA

Upstream 100 bases:

>100_bases
AATGCCTTGCACCTTTTTTGGCAGGATGGAGAAAAGCGCCTGGCGCTGAGCGACAAGGCACTCCTTGGCCAACGTTTAAT
AGACGAGATAGTCAGCCGTT

Downstream 100 bases:

>100_bases
GCGATACCCGATTGCCGACAGGGAAACGTCGCCTGAAATCTTGCCACACCGGAGAGAAGGACAGCTCTACGGCAAGGTTT
GCCCGGCTACGATTTCAGCG

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MMKKIDVKILDPRIGKDFPLPTYATPGSAGLDLRACLDSAVELAPGETQLLPTGLAIHIADTDLAAVILPRSGLGHKHGV
VLGNLVGLIDSDYQGQLMVSVWNRGQKSFTIEPGERIAQMVFVPVVQAEFNLVEEFDSSERGAGGFGHSGRH

Sequences:

>Translated_152_residues
MMKKIDVKILDPRIGKDFPLPTYATPGSAGLDLRACLDSAVELAPGETQLLPTGLAIHIADTDLAAVILPRSGLGHKHGV
VLGNLVGLIDSDYQGQLMVSVWNRGQKSFTIEPGERIAQMVFVPVVQAEFNLVEEFDSSERGAGGFGHSGRH
>Mature_152_residues
MMKKIDVKILDPRIGKDFPLPTYATPGSAGLDLRACLDSAVELAPGETQLLPTGLAIHIADTDLAAVILPRSGLGHKHGV
VLGNLVGLIDSDYQGQLMVSVWNRGQKSFTIEPGERIAQMVFVPVVQAEFNLVEEFDSSERGAGGFGHSGRH

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=140, Percent_Identity=32.8571428571429, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI4503423, Length=140, Percent_Identity=32.8571428571429, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI70906441, Length=140, Percent_Identity=32.8571428571429, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1790071, Length=151, Percent_Identity=84.7682119205298, Blast_Score=273, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI71988561, Length=152, Percent_Identity=34.2105263157895, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6319729, Length=133, Percent_Identity=33.8345864661654, Blast_Score=75, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24583610, Length=148, Percent_Identity=31.0810810810811, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19921126, Length=148, Percent_Identity=31.0810810810811, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_SERP5 (A8GLE7)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001481065.1
- ProteinModelPortal:   A8GLE7
- SMR:   A8GLE7
- STRING:   A8GLE7
- GeneID:   5602656
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4844
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- ProtClustDB:   PRK00601
- BioCyc:   SPRO399741:SPRO_4844-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16260; Mature: 16260

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: BINDING 84-84 BINDING 98-98

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKIDVKILDPRIGKDFPLPTYATPGSAGLDLRACLDSAVELAPGETQLLPTGLAIHIA
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCEEEEEE
DTDLAAVILPRSGLGHKHGVVLGNLVGLIDSDYQGQLMVSVWNRGQKSFTIEPGERIAQM
CCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCEEEEECCHHHHHHH
VFVPVVQAEFNLVEEFDSSERGAGGFGHSGRH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMKKIDVKILDPRIGKDFPLPTYATPGSAGLDLRACLDSAVELAPGETQLLPTGLAIHIA
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCEEEEEE
DTDLAAVILPRSGLGHKHGVVLGNLVGLIDSDYQGQLMVSVWNRGQKSFTIEPGERIAQM
CCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCEEEEECCHHHHHHH
VFVPVVQAEFNLVEEFDSSERGAGGFGHSGRH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA