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The map label for this gene is rfaD

Identifier: 157373057

GI number: 157373057

Start: 5345087

End: 5346016

Strand: Direct

Name: rfaD

Synonym: Spro_4825

Alternate gene names: 157373057

Gene position: 5345087-5346016 (Clockwise)

Preceding gene: 157373054

Following gene: 157373058

Centisome position: 98.1

GC content: 54.95

Gene sequence:

>930_bases
ATGATTATCGTCACTGGTGGCGCCGGCATGATCGGCAGCAATATCATTAAGGCGCTGAATGACACAGGGTATCGCGATAT
CCTGGTGGTAGATAACCTGAAAGACGGCACCAAATTCGCCAACCTGGTTGATCTCGATATCGCCGACTATATCGATAAAG
AAGATTTCATCGCCAACATCATTGCCGGTGACGATCTGGGCGAAATCGACGCCATCTTCCATGAGGGCGCCTGCTCTTCC
ACCACCGAGTGGGACGGCAAGTACATGATGGACAATAACTATCAGTACTCCAAAGACCTGCTGCACTACTGCCTGGATCG
TGAGATCCCGTTCCTGTATGCCTCGTCTGCTGCCACCTACGGCGGCCGCGAAGAATTTATCGAAGAGCGCCAATTTGAAG
CACCGCTGAACGTCTACGGCTACTCAAAATTCCTGTTCGACCAATATGTCCGTGAAATTCTGCCTGAGGCAGAATCACAG
ATCTGCGGCTTCCGTTACTTCAACGTCTACGGCCCGCGCGAAGGCCACAAAGGCAGCATGGCCAGCGTGGCGTTCCACCT
GAACGGCCAGATCAACCGTGGCGAGAACCCAAAACTGTTCGACGGCAGTCAAGACTTCAAGCGTGACTTCATCTACGTCG
GCGACGTGGCGGCAGTGAACCTGTGGTTCTTGAAAAGCGGCGTATCCGGCATCTTCAACTGCGGCACCGGCCGTGCGGAA
ACCTTCCAGGCGGTCGCCGATGCCGTGGTCGACTTCCACCAGAAAGGTGCGGTAGAAAACATTCCGTTCCCGGAAAAACT
GAAGGGTCGCTACCAGGCATTCACTCAGGCCGACCTGACCAAACTGCGCGCTGCCGGCTATGACGCGCCGTTCAAAACGG
TCGCCGAAGGCGTGAAAGAGTACATGGCCTGGTTAAACCGCACCGCTTAA

Upstream 100 bases:

>100_bases
CGGAGCCCGTCCGATGTTTGCGACGACATGAAATGCTATTATAAGCGACATTGTGTGTGGGACAGCCTGTCCCACCGGGC
AGAATAACAGGGGTAACCCA

Downstream 100 bases:

>100_bases
GCTGAAGGGATTTGACGTAGGGTATGAAAATACTGGTTATCGGCCCTTCTTGGGTTGGCGATATGATGATGTCGCAAAGT
CTCTACCGCACCCTGAAGGC

Product: ADP-L-glycero-D-mannoheptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MIIVTGGAGMIGSNIIKALNDTGYRDILVVDNLKDGTKFANLVDLDIADYIDKEDFIANIIAGDDLGEIDAIFHEGACSS
TTEWDGKYMMDNNYQYSKDLLHYCLDREIPFLYASSAATYGGREEFIEERQFEAPLNVYGYSKFLFDQYVREILPEAESQ
ICGFRYFNVYGPREGHKGSMASVAFHLNGQINRGENPKLFDGSQDFKRDFIYVGDVAAVNLWFLKSGVSGIFNCGTGRAE
TFQAVADAVVDFHQKGAVENIPFPEKLKGRYQAFTQADLTKLRAAGYDAPFKTVAEGVKEYMAWLNRTA

Sequences:

>Translated_309_residues
MIIVTGGAGMIGSNIIKALNDTGYRDILVVDNLKDGTKFANLVDLDIADYIDKEDFIANIIAGDDLGEIDAIFHEGACSS
TTEWDGKYMMDNNYQYSKDLLHYCLDREIPFLYASSAATYGGREEFIEERQFEAPLNVYGYSKFLFDQYVREILPEAESQ
ICGFRYFNVYGPREGHKGSMASVAFHLNGQINRGENPKLFDGSQDFKRDFIYVGDVAAVNLWFLKSGVSGIFNCGTGRAE
TFQAVADAVVDFHQKGAVENIPFPEKLKGRYQAFTQADLTKLRAAGYDAPFKTVAEGVKEYMAWLNRTA
>Mature_309_residues
MIIVTGGAGMIGSNIIKALNDTGYRDILVVDNLKDGTKFANLVDLDIADYIDKEDFIANIIAGDDLGEIDAIFHEGACSS
TTEWDGKYMMDNNYQYSKDLLHYCLDREIPFLYASSAATYGGREEFIEERQFEAPLNVYGYSKFLFDQYVREILPEAESQ
ICGFRYFNVYGPREGHKGSMASVAFHLNGQINRGENPKLFDGSQDFKRDFIYVGDVAAVNLWFLKSGVSGIFNCGTGRAE
TFQAVADAVVDFHQKGAVENIPFPEKLKGRYQAFTQADLTKLRAAGYDAPFKTVAEGVKEYMAWLNRTA

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily

Homologues:

Organism=Escherichia coli, GI1790049, Length=310, Percent_Identity=81.6129032258064, Blast_Score=538, Evalue=1e-154,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): HLDD_SERP5 (A8GLC8)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001481046.1
- ProteinModelPortal:   A8GLC8
- SMR:   A8GLC8
- STRING:   A8GLC8
- GeneID:   5606103
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4825
- eggNOG:   COG0451
- HOGENOM:   HBG755066
- OMA:   GFIGSAM
- ProtClustDB:   PRK11150
- BioCyc:   SPRO399741:SPRO_4825-MONOMER
- HAMAP:   MF_01601
- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10366:SF29
- TIGRFAMs:   TIGR02197

Pfam domain/function: PF01370 Epimerase

EC number: =5.1.3.20

Molecular weight: Translated: 34549; Mature: 34549

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: ACT_SITE 139-139 ACT_SITE 177-177 BINDING 38-38 BINDING 53-53 BINDING 92-92 BINDING 143-143 BINDING 168-168 BINDING 169-169 BINDING 177-177 BINDING 179-179 BINDING 186-186 BINDING 208-208 BINDING 271-271

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIVTGGAGMIGSNIIKALNDTGYRDILVVDNLKDGTKFANLVDLDIADYIDKEDFIANI
CEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHEEECHHHHHCCHHHHHHHH
IAGDDLGEIDAIFHEGACSSTTEWDGKYMMDNNYQYSKDLLHYCLDREIPFLYASSAATY
HCCCCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCC
GGREEFIEERQFEAPLNVYGYSKFLFDQYVREILPEAESQICGFRYFNVYGPREGHKGSM
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCEEEEEEECCCCCCCCCE
ASVAFHLNGQINRGENPKLFDGSQDFKRDFIYVGDVAAVNLWFLKSGVSGIFNCGTGRAE
EEEEEEECCEECCCCCCCEECCCHHHHHCEEEECCHHHHHHHHHHHCCHHHEECCCCCHH
TFQAVADAVVDFHQKGAVENIPFPEKLKGRYQAFTQADLTKLRAAGYDAPFKTVAEGVKE
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
YMAWLNRTA
HHHHHHCCC
>Mature Secondary Structure
MIIVTGGAGMIGSNIIKALNDTGYRDILVVDNLKDGTKFANLVDLDIADYIDKEDFIANI
CEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHEEECHHHHHCCHHHHHHHH
IAGDDLGEIDAIFHEGACSSTTEWDGKYMMDNNYQYSKDLLHYCLDREIPFLYASSAATY
HCCCCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCC
GGREEFIEERQFEAPLNVYGYSKFLFDQYVREILPEAESQICGFRYFNVYGPREGHKGSM
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCEEEEEEECCCCCCCCCE
ASVAFHLNGQINRGENPKLFDGSQDFKRDFIYVGDVAAVNLWFLKSGVSGIFNCGTGRAE
EEEEEEECCEECCCCCCCEECCCHHHHHCEEEECCHHHHHHHHHHHCCHHHEECCCCCHH
TFQAVADAVVDFHQKGAVENIPFPEKLKGRYQAFTQADLTKLRAAGYDAPFKTVAEGVKE
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
YMAWLNRTA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA