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The map label for this gene is glpF [H]

Identifier: 157373029

GI number: 157373029

Start: 5316390

End: 5317235

Strand: Direct

Name: glpF [H]

Synonym: Spro_4797

Alternate gene names: 157373029

Gene position: 5316390-5317235 (Clockwise)

Preceding gene: 157373027

Following gene: 157373030

Centisome position: 97.57

GC content: 58.27

Gene sequence:

>846_bases
ATGAGCCAAACCACCAGTCCGACCCTAAAAGGCCAATGTATCGCCGAGTTTCTCGGCACCGGCCTGTTGATATTTTTTGG
CGTAGGCTGCGTTGCCGCGTTGAAACTGGCTGGCGCCAGCTTCGGCCAGTGGGAAATCAGTATCATTTGGGGCCTGGGCG
TTGCCATGGCCATCTATCTGACCGCTGCCATCTCTGGTGCGCATCTCAACCCGGCAGTCACCATCGCACTGTGGCTGTTC
GCCTGCTTTGACGGACGCAAAGTATTGCCTTACATCGTCGCGCAGATCGCCGGAGCCTTCTGCGCCGCAGCGCTGGTCTA
CGGGCTGTATTACAACCTGTTCTTCGATTTTGAAGCGGCCAACCATATGGTACGTGGCAGCGACGAAAGCCTGGCCCTGG
CCGGCATCTTCTCCACCTACCCTAATGCACACATCTCCGTGGGTCAGGCTTTCCTGGTTGAAACGGTGATTACCGCGATC
CTGATGTGCCTGATCCTGGCGCTGACCGACGACGGCAACGGCATTCCACGTGGCCCGCTGGCACCGCTGTTAATCGGTAT
TCTGATTGCCGTGATCGGCGCCTCTATGGGACCATTGACCGGCTTTGCGCTGAACCCGGCCCGTGACTTCGGTCCGAAAA
TGTTCGCTTATCTGGCCGGCTGGGGCAAAGTGGCCTTCACCGGTGCGCGTGATATCCCGTACTTCCTGGTGCCAATCTTT
GGCCCAATCATCGGTGCCTGCCTGGGTGCCTTCGGCTACCGCATGCTCATTGGCCGCAATCTGCCGTGTGACGTTTGCGT
AGAAGAAGAAAAACCCGCCGCCAAGGCTCAGCAGCGTAAAGCATGA

Upstream 100 bases:

>100_bases
AATAATTGGACGAGATGAAAAAAACTGTACCCATAGGCCAGATCGATACACACCCAGAGCCTATAAACCCATAACCGCCG
TTTTGCAGGACAACAACATT

Downstream 100 bases:

>100_bases
TTGGCACAGTTTTAAAACGGTTAACAACAGCAGGACTAAAATCATGACTGTAGAAAAAAAATACATTGTCGCTCTCGACC
AGGGAACTACCAGTTCACGT

Product: MIP family channel protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSQTTSPTLKGQCIAEFLGTGLLIFFGVGCVAALKLAGASFGQWEISIIWGLGVAMAIYLTAAISGAHLNPAVTIALWLF
ACFDGRKVLPYIVAQIAGAFCAAALVYGLYYNLFFDFEAANHMVRGSDESLALAGIFSTYPNAHISVGQAFLVETVITAI
LMCLILALTDDGNGIPRGPLAPLLIGILIAVIGASMGPLTGFALNPARDFGPKMFAYLAGWGKVAFTGARDIPYFLVPIF
GPIIGACLGAFGYRMLIGRNLPCDVCVEEEKPAAKAQQRKA

Sequences:

>Translated_281_residues
MSQTTSPTLKGQCIAEFLGTGLLIFFGVGCVAALKLAGASFGQWEISIIWGLGVAMAIYLTAAISGAHLNPAVTIALWLF
ACFDGRKVLPYIVAQIAGAFCAAALVYGLYYNLFFDFEAANHMVRGSDESLALAGIFSTYPNAHISVGQAFLVETVITAI
LMCLILALTDDGNGIPRGPLAPLLIGILIAVIGASMGPLTGFALNPARDFGPKMFAYLAGWGKVAFTGARDIPYFLVPIF
GPIIGACLGAFGYRMLIGRNLPCDVCVEEEKPAAKAQQRKA
>Mature_280_residues
SQTTSPTLKGQCIAEFLGTGLLIFFGVGCVAALKLAGASFGQWEISIIWGLGVAMAIYLTAAISGAHLNPAVTIALWLFA
CFDGRKVLPYIVAQIAGAFCAAALVYGLYYNLFFDFEAANHMVRGSDESLALAGIFSTYPNAHISVGQAFLVETVITAIL
MCLILALTDDGNGIPRGPLAPLLIGILIAVIGASMGPLTGFALNPARDFGPKMFAYLAGWGKVAFTGARDIPYFLVPIFG
PIIGACLGAFGYRMLIGRNLPCDVCVEEEKPAAKAQQRKA

Specific function: Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=276, Percent_Identity=38.4057971014493, Blast_Score=174, Evalue=7e-44,
Organism=Homo sapiens, GI4826645, Length=273, Percent_Identity=38.8278388278388, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI22538420, Length=277, Percent_Identity=36.8231046931408, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4502187, Length=266, Percent_Identity=35.3383458646617, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI310133356, Length=181, Percent_Identity=36.4640883977901, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI310114181, Length=181, Percent_Identity=36.4640883977901, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI86792455, Length=203, Percent_Identity=36.9458128078818, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI4502179, Length=245, Percent_Identity=32.2448979591837, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI37694062, Length=209, Percent_Identity=30.622009569378, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI45446752, Length=205, Percent_Identity=28.780487804878, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4502183, Length=247, Percent_Identity=32.3886639676113, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI6912506, Length=258, Percent_Identity=30.6201550387597, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1790362, Length=281, Percent_Identity=81.8505338078292, Blast_Score=469, Evalue=1e-134,
Organism=Escherichia coli, GI1787101, Length=212, Percent_Identity=32.0754716981132, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71994009, Length=261, Percent_Identity=35.632183908046, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI71992966, Length=249, Percent_Identity=35.7429718875502, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17533613, Length=276, Percent_Identity=32.9710144927536, Blast_Score=142, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32564052, Length=276, Percent_Identity=32.9710144927536, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17544068, Length=268, Percent_Identity=37.3134328358209, Blast_Score=140, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17531429, Length=260, Percent_Identity=34.2307692307692, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17531431, Length=260, Percent_Identity=34.2307692307692, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71992961, Length=249, Percent_Identity=32.1285140562249, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71993722, Length=265, Percent_Identity=25.6603773584906, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17558372, Length=262, Percent_Identity=30.1526717557252, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=216, Percent_Identity=38.8888888888889, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6322985, Length=224, Percent_Identity=32.5892857142857, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24762346, Length=259, Percent_Identity=29.3436293436293, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24762348, Length=259, Percent_Identity=29.3436293436293, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20130305, Length=259, Percent_Identity=29.3436293436293, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24762344, Length=259, Percent_Identity=29.3436293436293, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550503, Length=202, Percent_Identity=28.7128712871287, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652747, Length=253, Percent_Identity=29.6442687747036, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45551084, Length=253, Percent_Identity=29.6442687747036, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24762342, Length=262, Percent_Identity=26.7175572519084, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 29608; Mature: 29477

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTTSPTLKGQCIAEFLGTGLLIFFGVGCVAALKLAGASFGQWEISIIWGLGVAMAIYL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHH
TAAISGAHLNPAVTIALWLFACFDGRKVLPYIVAQIAGAFCAAALVYGLYYNLFFDFEAA
HHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NHMVRGSDESLALAGIFSTYPNAHISVGQAFLVETVITAILMCLILALTDDGNGIPRGPL
CHHHCCCCCCEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
APLLIGILIAVIGASMGPLTGFALNPARDFGPKMFAYLAGWGKVAFTGARDIPYFLVPIF
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHH
GPIIGACLGAFGYRMLIGRNLPCDVCVEEEKPAAKAQQRKA
HHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHCCC
>Mature Secondary Structure 
SQTTSPTLKGQCIAEFLGTGLLIFFGVGCVAALKLAGASFGQWEISIIWGLGVAMAIYL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHH
TAAISGAHLNPAVTIALWLFACFDGRKVLPYIVAQIAGAFCAAALVYGLYYNLFFDFEAA
HHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NHMVRGSDESLALAGIFSTYPNAHISVGQAFLVETVITAILMCLILALTDDGNGIPRGPL
CHHHCCCCCCEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
APLLIGILIAVIGASMGPLTGFALNPARDFGPKMFAYLAGWGKVAFTGARDIPYFLVPIF
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHH
GPIIGACLGAFGYRMLIGRNLPCDVCVEEEKPAAKAQQRKA
HHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]