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The map label for this gene is dnaG [H]

Identifier: 157372532

GI number: 157372532

Start: 4765104

End: 4766852

Strand: Direct

Name: dnaG [H]

Synonym: Spro_4299

Alternate gene names: 157372532

Gene position: 4765104-4766852 (Clockwise)

Preceding gene: 157372531

Following gene: 157372533

Centisome position: 87.45

GC content: 54.77

Gene sequence:

>1749_bases
ATGGCTGGACGAATTCCGCGTGTATTTATCAATGACTTGCTGGCTCGCACCGACATCGTCGATCTGATCGACGCCCGGGT
AAAGCTCAAGAAGCAGGGCAAGAACTATCACGCGTGCTGTCCGTTCCACCACGAAAAAACGCCTTCATTTACCGTTAATG
GTGAAAAGCAGTTTTATCACTGTTTTGGTTGTGGTGCGCACGGTAACGCCGTCGACTTTCTGATGAATTACGACCGACTT
GAGTTTGTCGAAACCATCGAAGAGCTGGCAACCATGCACGGACTGGAAGTGCCTTACGAGGCAGGTTCCGGGCCGACCCA
AATCGAACGTCATCAGCGCCAAAGCCTGTATCAACTGATGGAACAGCTCAGTTCGTTCTATCAACAGTCACTGCATCAAC
CTTCCGGTGCGCCGGCGCGTAATTATCTGCAACAGCGCGGGTTGAGTGACGATGTTATCCGCCATTTTGCCATCGGCTTT
GCACCTGCCGGTTGGGATAATGCCTTAAAGCGTTTTGGTCGCGACGCCGACAGCCGTAGTGCATTGAACGATGCCGGCAT
GCTGGTGACTAACGATCAAGGACGCTCGTACGATCGTTTCCGTGAACGCGTAATGTTCCCTATTCGCGACAAGCGTGGGC
GAGTGATCGCCTTTGGCGGACGCGTGTTGGGTGACGGCATGCCGAAATACCTGAACTCGCCGGAAACTGAAGTATTCCAC
AAGGGTCGTCAACTGTACGGCCTGTATGAAGCGCAGCAGAACCACCCTACCCCGCAGCGTTTGCTGGTAGTGGAAGGCTA
TATGGACGTGGTGGCGTTGGCGCAATACGGCATTGATTATGCCGTCGCCTCGCTCGGAACCTCAACGACGGCTGAACATA
TTCAGCTGCTGTTCCGCGCCACCGACAACGTGGTCTGTTGCTATGACGGTGACCGGGCCGGTCGTGAAGCGGCCTGGCGC
GCACTGGAAACCGCGCTGCCCTATCTCAATGACGGGCGTCAGCTGCGGTTTATGTTTTTGCCTGATGGCGAAGACCCGGA
CACATTGGTACGCAAAGAAGGCAAAGAGGCCTTCGAACAGCGGATGGAGCTGGCGCAGCCGCTTTCCACTTTCCTGTTCG
AAACCCTGATGCCACAGGTTGATCTGAGCAGCCCGGACGGCCGCGCCAAGCTGAGCACGCTGGCGCTACCGCTGATTACT
CAGGTGCCGGGCGAAACGTTGCGCTTGTACCTGCGTCAACAGCTCGGCAGCAAGCTTGGCCTGCTCGATGACAGCCAGCT
AGACAAGCTGATGCCCAAACAGGCTGAAAATGCGAATACCTATCAGGCACCCCAGCTAAAACGCACAACCATGCGTATAC
TAATAGGGTTACTGGTACAAAATCCGCAGTTGGCTACACTTATCCCTTCACTCGAGGGATTGGAGCAGACCAAACAGGCC
GGTTTACCGCTGTTTGTCGAGTTGGTGCAGACCTGTTTAGCTCAGCCAGGCCTGACTACCGGCCAGTTGTTGGAGCAGTA
TCGCGACAACAAATTGAGCCAGCAGCTTGAAACCCTGGCGACATGGAACCATATGATCGTTGAGGAAAGGGTCGAACAGA
CCTTTTTGGACACCTTGGCCAGCCTATATGACTCGGTACTGGAGCAACGGCTGGAGACATTGATCGCCCAGGCCAGAACA
CATGGCCTCAGCCCGGAAGAACGTGAAGAAGTCCGTTCTCTTAACCAAGTGCTAGCGAAGAAAAACTGA

Upstream 100 bases:

>100_bases
TTTAAAAATCGCGCAGACCGAGTAGTTGCATACGAAGGCCGTGCTTTCCGAAAGGAATGCGCGGCTTGTTCTCGTTTATA
GGCTAGTAAAGTAAGGGCTT

Downstream 100 bases:

>100_bases
ATACCTAAACGGCTTAAGTGCCGATAAATGCGAGGGCAGAGCCCTGCAATATGCCGCCACAGTGGGCCGCGGCAACAACA
AAAACGCCCCAAATGCTATT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 581

Protein sequence:

>582_residues
MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNYHACCPFHHEKTPSFTVNGEKQFYHCFGCGAHGNAVDFLMNYDRL
EFVETIEELATMHGLEVPYEAGSGPTQIERHQRQSLYQLMEQLSSFYQQSLHQPSGAPARNYLQQRGLSDDVIRHFAIGF
APAGWDNALKRFGRDADSRSALNDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIAFGGRVLGDGMPKYLNSPETEVFH
KGRQLYGLYEAQQNHPTPQRLLVVEGYMDVVALAQYGIDYAVASLGTSTTAEHIQLLFRATDNVVCCYDGDRAGREAAWR
ALETALPYLNDGRQLRFMFLPDGEDPDTLVRKEGKEAFEQRMELAQPLSTFLFETLMPQVDLSSPDGRAKLSTLALPLIT
QVPGETLRLYLRQQLGSKLGLLDDSQLDKLMPKQAENANTYQAPQLKRTTMRILIGLLVQNPQLATLIPSLEGLEQTKQA
GLPLFVELVQTCLAQPGLTTGQLLEQYRDNKLSQQLETLATWNHMIVEERVEQTFLDTLASLYDSVLEQRLETLIAQART
HGLSPEEREEVRSLNQVLAKKN

Sequences:

>Translated_582_residues
MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNYHACCPFHHEKTPSFTVNGEKQFYHCFGCGAHGNAVDFLMNYDRL
EFVETIEELATMHGLEVPYEAGSGPTQIERHQRQSLYQLMEQLSSFYQQSLHQPSGAPARNYLQQRGLSDDVIRHFAIGF
APAGWDNALKRFGRDADSRSALNDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIAFGGRVLGDGMPKYLNSPETEVFH
KGRQLYGLYEAQQNHPTPQRLLVVEGYMDVVALAQYGIDYAVASLGTSTTAEHIQLLFRATDNVVCCYDGDRAGREAAWR
ALETALPYLNDGRQLRFMFLPDGEDPDTLVRKEGKEAFEQRMELAQPLSTFLFETLMPQVDLSSPDGRAKLSTLALPLIT
QVPGETLRLYLRQQLGSKLGLLDDSQLDKLMPKQAENANTYQAPQLKRTTMRILIGLLVQNPQLATLIPSLEGLEQTKQA
GLPLFVELVQTCLAQPGLTTGQLLEQYRDNKLSQQLETLATWNHMIVEERVEQTFLDTLASLYDSVLEQRLETLIAQART
HGLSPEEREEVRSLNQVLAKKN
>Mature_581_residues
AGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNYHACCPFHHEKTPSFTVNGEKQFYHCFGCGAHGNAVDFLMNYDRLE
FVETIEELATMHGLEVPYEAGSGPTQIERHQRQSLYQLMEQLSSFYQQSLHQPSGAPARNYLQQRGLSDDVIRHFAIGFA
PAGWDNALKRFGRDADSRSALNDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIAFGGRVLGDGMPKYLNSPETEVFHK
GRQLYGLYEAQQNHPTPQRLLVVEGYMDVVALAQYGIDYAVASLGTSTTAEHIQLLFRATDNVVCCYDGDRAGREAAWRA
LETALPYLNDGRQLRFMFLPDGEDPDTLVRKEGKEAFEQRMELAQPLSTFLFETLMPQVDLSSPDGRAKLSTLALPLITQ
VPGETLRLYLRQQLGSKLGLLDDSQLDKLMPKQAENANTYQAPQLKRTTMRILIGLLVQNPQLATLIPSLEGLEQTKQAG
LPLFVELVQTCLAQPGLTTGQLLEQYRDNKLSQQLETLATWNHMIVEERVEQTFLDTLASLYDSVLEQRLETLIAQARTH
GLSPEEREEVRSLNQVLAKKN

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=581, Percent_Identity=77.9690189328744, Blast_Score=952, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65734; Mature: 65603

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNYHACCPFHHEKTPSFTVNGEKQFYH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCEEECCCCCEEE
CFGCGAHGNAVDFLMNYDRLEFVETIEELATMHGLEVPYEAGSGPTQIERHQRQSLYQLM
EEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
EQLSSFYQQSLHQPSGAPARNYLQQRGLSDDVIRHFAIGFAPAGWDNALKRFGRDADSRS
HHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHH
ALNDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIAFGGRVLGDGMPKYLNSPETEVFH
HHCCCCEEEECCCCCCHHHHHHHCCCCCCCCCCCEEEECCCHHCCCCHHHCCCCHHHHHH
KGRQLYGLYEAQQNHPTPQRLLVVEGYMDVVALAQYGIDYAVASLGTSTTAEHIQLLFRA
CCHHHHHHHHHHCCCCCHHHEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
TDNVVCCYDGDRAGREAAWRALETALPYLNDGRQLRFMFLPDGEDPDTLVRKEGKEAFEQ
CCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
RMELAQPLSTFLFETLMPQVDLSSPDGRAKLSTLALPLITQVPGETLRLYLRQQLGSKLG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHCC
LLDDSQLDKLMPKQAENANTYQAPQLKRTTMRILIGLLVQNPQLATLIPSLEGLEQTKQA
CCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
GLPLFVELVQTCLAQPGLTTGQLLEQYRDNKLSQQLETLATWNHMIVEERVEQTFLDTLA
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLYDSVLEQRLETLIAQARTHGLSPEEREEVRSLNQVLAKKN
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNYHACCPFHHEKTPSFTVNGEKQFYH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCEEECCCCCEEE
CFGCGAHGNAVDFLMNYDRLEFVETIEELATMHGLEVPYEAGSGPTQIERHQRQSLYQLM
EEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
EQLSSFYQQSLHQPSGAPARNYLQQRGLSDDVIRHFAIGFAPAGWDNALKRFGRDADSRS
HHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHH
ALNDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIAFGGRVLGDGMPKYLNSPETEVFH
HHCCCCEEEECCCCCCHHHHHHHCCCCCCCCCCCEEEECCCHHCCCCHHHCCCCHHHHHH
KGRQLYGLYEAQQNHPTPQRLLVVEGYMDVVALAQYGIDYAVASLGTSTTAEHIQLLFRA
CCHHHHHHHHHHCCCCCHHHEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
TDNVVCCYDGDRAGREAAWRALETALPYLNDGRQLRFMFLPDGEDPDTLVRKEGKEAFEQ
CCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
RMELAQPLSTFLFETLMPQVDLSSPDGRAKLSTLALPLITQVPGETLRLYLRQQLGSKLG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHCC
LLDDSQLDKLMPKQAENANTYQAPQLKRTTMRILIGLLVQNPQLATLIPSLEGLEQTKQA
CCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
GLPLFVELVQTCLAQPGLTTGQLLEQYRDNKLSQQLETLATWNHMIVEERVEQTFLDTLA
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLYDSVLEQRLETLIAQARTHGLSPEEREEVRSLNQVLAKKN
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]