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The map label for this gene is scrR [H]

Identifier: 157372440

GI number: 157372440

Start: 4665518

End: 4666561

Strand: Direct

Name: scrR [H]

Synonym: Spro_4207

Alternate gene names: 157372440

Gene position: 4665518-4666561 (Clockwise)

Preceding gene: 157372439

Following gene: 157372445

Centisome position: 85.62

GC content: 59.87

Gene sequence:

>1044_bases
ATGCCGTCAGTGAAAAAAAATAAACGCATTACCATCAGTGATATTGCCGCACTGGCCGGCGTGTCGAAATCGACCGCCAG
CCTGGTGCTTAACGGTCGCAGCAAGGAATATCGGGTGTCCGACGACACCCGCGATCGCATCCTGGCACTGGCCGGTGAGC
ACCACTACCAGCCCAGCTTCCACGCCCGTTCACTGCGTTCTTCCCGCAGCCATACCCTGGGGCTGGTGGTGCCGGAAATG
ACCAACTACGGTTTTGCGGTGATTTCACGCGAGCTGGAAACCCTGTGCCGCGAGGCCGGTTTGCAGTTGCTGATCGCCTG
TACCGATGAAAACCCGGCGCAGGAAATGATGGCGGTGAACAGTCTGGTACAACGTCAGGTCGACGGTCTGATTGTCGCCT
CCAGCCAGCTTAACGATGCGGAATACCAAAAGATTAACGCCGGCCTGCCGGTGGTGCAGATGGACCGGCTGATCGTCGGC
TCCGACCTGCCGCTGGTGATCACCGACTCGCTGACCTCGACCGCCACGCTGGTGGAAAACGTCGCGCGTCAGCATCCGGA
CGAATTCTATTTCCTTGGCGGCCAACCCCGCATTTCACCCACGCGCGATCGGCTGGCCGGTTTCCAACTCGGTCTGGAAC
GTGCCGGGGTGGTGTGTAAACCGGAATGGATCATCAACGGTAATTATCACCCCAGTTCCGGCTACGAGATGTTCGCCCAA
CTCTGTGCGCAACTGGGCCGACCGCCCAAGGCGCTGTTTACCGCCGCCTGCGGCCTGCTGGAAGGGGTGCTGCGCTACCT
GAATCAGCACCAGTTGATGGAGAGCGATCTGCATCTGTGCAGCTTTGACGACCACTACCTGTTCGACGGACTAACGCTGA
AAATCGACACCGTGGCGCAAGACTGCCGCGCCCTGGCCCAACACAGCTTCGACATGGTTAGCGCGCTGATTGACGAGCGG
CCATTAGAGCAGAACGCGCTATACCTGCCGGGCAGCATCCACTGGCGCCACGCCGGTTCGAAGGCATTGCTCGGTCAGCC
TTAG

Upstream 100 bases:

>100_bases
GCAACCACAACTGCACCTGAGCGGTAAAACCCAACTGGCGCTCGAGCACTGGTCGCTGACGCCATGCATGCTAGAATGAC
GCATCCTTCGATGAATTCAG

Downstream 100 bases:

>100_bases
GCTTTTCTTGTCCCGGTTAAGGAGTTCGTCATAAGTCGTGAGCATGGCAAACAGGTGCTTACCGGGGCAGAGGTGATTGC
GCTACGGCACATGCTCGTGG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Scr operon regulatory protein [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MPSVKKNKRITISDIAALAGVSKSTASLVLNGRSKEYRVSDDTRDRILALAGEHHYQPSFHARSLRSSRSHTLGLVVPEM
TNYGFAVISRELETLCREAGLQLLIACTDENPAQEMMAVNSLVQRQVDGLIVASSQLNDAEYQKINAGLPVVQMDRLIVG
SDLPLVITDSLTSTATLVENVARQHPDEFYFLGGQPRISPTRDRLAGFQLGLERAGVVCKPEWIINGNYHPSSGYEMFAQ
LCAQLGRPPKALFTAACGLLEGVLRYLNQHQLMESDLHLCSFDDHYLFDGLTLKIDTVAQDCRALAQHSFDMVSALIDER
PLEQNALYLPGSIHWRHAGSKALLGQP

Sequences:

>Translated_347_residues
MPSVKKNKRITISDIAALAGVSKSTASLVLNGRSKEYRVSDDTRDRILALAGEHHYQPSFHARSLRSSRSHTLGLVVPEM
TNYGFAVISRELETLCREAGLQLLIACTDENPAQEMMAVNSLVQRQVDGLIVASSQLNDAEYQKINAGLPVVQMDRLIVG
SDLPLVITDSLTSTATLVENVARQHPDEFYFLGGQPRISPTRDRLAGFQLGLERAGVVCKPEWIINGNYHPSSGYEMFAQ
LCAQLGRPPKALFTAACGLLEGVLRYLNQHQLMESDLHLCSFDDHYLFDGLTLKIDTVAQDCRALAQHSFDMVSALIDER
PLEQNALYLPGSIHWRHAGSKALLGQP
>Mature_346_residues
PSVKKNKRITISDIAALAGVSKSTASLVLNGRSKEYRVSDDTRDRILALAGEHHYQPSFHARSLRSSRSHTLGLVVPEMT
NYGFAVISRELETLCREAGLQLLIACTDENPAQEMMAVNSLVQRQVDGLIVASSQLNDAEYQKINAGLPVVQMDRLIVGS
DLPLVITDSLTSTATLVENVARQHPDEFYFLGGQPRISPTRDRLAGFQLGLERAGVVCKPEWIINGNYHPSSGYEMFAQL
CAQLGRPPKALFTAACGLLEGVLRYLNQHQLMESDLHLCSFDDHYLFDGLTLKIDTVAQDCRALAQHSFDMVSALIDERP
LEQNALYLPGSIHWRHAGSKALLGQP

Specific function: Repressor for the scr operon. Binds D-fructose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786268, Length=313, Percent_Identity=32.9073482428115, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1790194, Length=323, Percent_Identity=28.4829721362229, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1787948, Length=312, Percent_Identity=27.8846153846154, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1790369, Length=303, Percent_Identity=27.3927392739274, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789202, Length=332, Percent_Identity=24.3975903614458, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1789068, Length=279, Percent_Identity=26.8817204301075, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787580, Length=325, Percent_Identity=25.2307692307692, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788474, Length=279, Percent_Identity=25.8064516129032, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787906, Length=220, Percent_Identity=25.9090909090909, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786540, Length=333, Percent_Identity=25.2252252252252, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1790715, Length=151, Percent_Identity=31.1258278145695, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789456, Length=285, Percent_Identity=28.0701754385965, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI48994940, Length=150, Percent_Identity=31.3333333333333, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012781
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38280; Mature: 38149

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSVKKNKRITISDIAALAGVSKSTASLVLNGRSKEYRVSDDTRDRILALAGEHHYQPSF
CCCCCCCCCEEHHHHHHHHCCCCCCEEEEEECCCCCEECCCCCHHHEEEEECCCCCCCCH
HARSLRSSRSHTLGLVVPEMTNYGFAVISRELETLCREAGLQLLIACTDENPAQEMMAVN
HHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
SLVQRQVDGLIVASSQLNDAEYQKINAGLPVVQMDRLIVGSDLPLVITDSLTSTATLVEN
HHHHHHHCCEEEECCCCCCHHHHHHCCCCCCEEECCEEECCCCCEEEECCCHHHHHHHHH
VARQHPDEFYFLGGQPRISPTRDRLAGFQLGLERAGVVCKPEWIINGNYHPSSGYEMFAQ
HHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCEECCEEEECCCCCCCCHHHHHHH
LCAQLGRPPKALFTAACGLLEGVLRYLNQHQLMESDLHLCSFDDHYLFDGLTLKIDTVAQ
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEECCEEEEEHHHHH
DCRALAQHSFDMVSALIDERPLEQNALYLPGSIHWRHAGSKALLGQP
HHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
PSVKKNKRITISDIAALAGVSKSTASLVLNGRSKEYRVSDDTRDRILALAGEHHYQPSF
CCCCCCCCEEHHHHHHHHCCCCCCEEEEEECCCCCEECCCCCHHHEEEEECCCCCCCCH
HARSLRSSRSHTLGLVVPEMTNYGFAVISRELETLCREAGLQLLIACTDENPAQEMMAVN
HHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
SLVQRQVDGLIVASSQLNDAEYQKINAGLPVVQMDRLIVGSDLPLVITDSLTSTATLVEN
HHHHHHHCCEEEECCCCCCHHHHHHCCCCCCEEECCEEECCCCCEEEECCCHHHHHHHHH
VARQHPDEFYFLGGQPRISPTRDRLAGFQLGLERAGVVCKPEWIINGNYHPSSGYEMFAQ
HHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCEECCEEEECCCCCCCCHHHHHHH
LCAQLGRPPKALFTAACGLLEGVLRYLNQHQLMESDLHLCSFDDHYLFDGLTLKIDTVAQ
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEECCEEEEEHHHHH
DCRALAQHSFDMVSALIDERPLEQNALYLPGSIHWRHAGSKALLGQP
HHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8412665 [H]