Click here to switch to the map view.

The map label for this gene is scrB [H]

Identifier: 157372439

GI number: 157372439

Start: 4664087

End: 4665496

Strand: Direct

Name: scrB [H]

Synonym: Spro_4206

Alternate gene names: 157372439

Gene position: 4664087-4665496 (Clockwise)

Preceding gene: 157372438

Following gene: 157372440

Centisome position: 85.6

GC content: 60.57

Gene sequence:

>1410_bases
ATGGAAGAATTGAATTTGATGAAACAGGCCGTTCAGGCGTTGATGCGCGACATGCCAAAGGTACTGCGTGCGCCGCACCG
CCCGGCGTGGCATCTGGCGCCCAGCGTCGGGTTGCTCAACGATCCCAACGGATTCATTCAGCATAACGGCGTCTACCATC
TGTTCTATCAGTGGAACCCGCTGGGTTGCGATCACCGCAACAAGTGTTGGGGCCATTGGCAGTCGACGGATCTGGTCAAC
TGGCAGCATCAGCCGGTGGCACTGGTGCCGGGAGCCTGCTACGACAGCCACGGCTGTTATTCCGGATCGGCAGTGGTGGC
GGACGGCAAAATCATGCTGGCGTATACCGGCAACGTCAAATACCCCGACGGCTCCCGCACCGCCTACCAGTGCCTGGCGC
AAGAAAATGCCCAGGGTGGCTATGACAAACTTGGCCCGGTATTACCGCTGCCGCCGGGCTATAGCGGCCACGTGCGCGAT
CCGAAGGTTTGGCGTCATCAGGACGACTGGTACATGGTGCTCGGCGCTCGCGACCTGCAAGACCGTGGCAAAGTGCTGCT
GTTGCGCTCCGCCGATCTGCACGGTTGGCAGTTGCTGGGAGAGATTGCCGGTTCACAGCTGAACGGCATCGGCGATTTCG
GCTATATGTGGGAATGCCCGGACCTGTTCACACTGGACGGGGTCGAGATACTGATCTGTTGCCCACAGGGGCTGGCGGCG
CAGGCCGAGCAGTATCTCAACGTTTATCAGGCGGGCTATTTCACTGGCAAACTGGATTATCGGCAGGCCAGTTTCGAACA
TGGTGCCTTCCACGAGCTGGATCTCGGCTTCGAATTCTATGCGCCACAGACCACCCAGGCCGAAGACGGCCGCCGGTTGC
TGGTCGGCTGGATGGGCGTGCCGGAGCAGGACGAGGATTATCACCCGACGCTGCAGTACGGCTGGATCCACATCCTGACC
TGCCCGCGCGAACTGTCGCTGCATCAGGGCAAGCTTTACCAACAACCGGCGCGCGAACTGCAACAATTGCGCGGTGAAAG
CCGGCAATGGCAGGGGATCGCCGACGAAGCGCCATGTTGGCCCGTCGGCAGCGCGGAACTGCTGCTAACGCCAGAAGGGG
CATTCAGCGCCGATTTTGGCGGGGCAATGACGCTGCTCTGGGACGGTGCCTTGCTACGACTGACGCGCAATAATCTGCGT
AACGGGCAACCGGAACATCGCTACTGGCGCGGCAACCTGAGCCAGCTGCAGATCCTGTTTGACCGCTCCAGCGTGGAGAT
TTTTATCAACCACGGCGAGGCAGTGATGAGCGCACGCTACTTCCCCGGGCCGCAACCACAACTGCACCTGAGCGGTAAAA
CCCAACTGGCGCTCGAGCACTGGTCGCTGACGCCATGCATGCTAGAATGA

Upstream 100 bases:

>100_bases
AGGCCAGTTCGCTGGTCAGCTATATCATTGGTTTGGTGATCGCCTTCGGCAGCGCCTTTATTATCTCGTTGCTGCTGAAA
TACAAAACGGACAGCGAATA

Downstream 100 bases:

>100_bases
CGCATCCTTCGATGAATTCAGATGCCGTCAGTGAAAAAAAATAAACGCATTACCATCAGTGATATTGCCGCACTGGCCGG
CGTGTCGAAATCGACCGCCA

Product: sucrose-6-phosphate hydrolase

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MEELNLMKQAVQALMRDMPKVLRAPHRPAWHLAPSVGLLNDPNGFIQHNGVYHLFYQWNPLGCDHRNKCWGHWQSTDLVN
WQHQPVALVPGACYDSHGCYSGSAVVADGKIMLAYTGNVKYPDGSRTAYQCLAQENAQGGYDKLGPVLPLPPGYSGHVRD
PKVWRHQDDWYMVLGARDLQDRGKVLLLRSADLHGWQLLGEIAGSQLNGIGDFGYMWECPDLFTLDGVEILICCPQGLAA
QAEQYLNVYQAGYFTGKLDYRQASFEHGAFHELDLGFEFYAPQTTQAEDGRRLLVGWMGVPEQDEDYHPTLQYGWIHILT
CPRELSLHQGKLYQQPARELQQLRGESRQWQGIADEAPCWPVGSAELLLTPEGAFSADFGGAMTLLWDGALLRLTRNNLR
NGQPEHRYWRGNLSQLQILFDRSSVEIFINHGEAVMSARYFPGPQPQLHLSGKTQLALEHWSLTPCMLE

Sequences:

>Translated_469_residues
MEELNLMKQAVQALMRDMPKVLRAPHRPAWHLAPSVGLLNDPNGFIQHNGVYHLFYQWNPLGCDHRNKCWGHWQSTDLVN
WQHQPVALVPGACYDSHGCYSGSAVVADGKIMLAYTGNVKYPDGSRTAYQCLAQENAQGGYDKLGPVLPLPPGYSGHVRD
PKVWRHQDDWYMVLGARDLQDRGKVLLLRSADLHGWQLLGEIAGSQLNGIGDFGYMWECPDLFTLDGVEILICCPQGLAA
QAEQYLNVYQAGYFTGKLDYRQASFEHGAFHELDLGFEFYAPQTTQAEDGRRLLVGWMGVPEQDEDYHPTLQYGWIHILT
CPRELSLHQGKLYQQPARELQQLRGESRQWQGIADEAPCWPVGSAELLLTPEGAFSADFGGAMTLLWDGALLRLTRNNLR
NGQPEHRYWRGNLSQLQILFDRSSVEIFINHGEAVMSARYFPGPQPQLHLSGKTQLALEHWSLTPCMLE
>Mature_469_residues
MEELNLMKQAVQALMRDMPKVLRAPHRPAWHLAPSVGLLNDPNGFIQHNGVYHLFYQWNPLGCDHRNKCWGHWQSTDLVN
WQHQPVALVPGACYDSHGCYSGSAVVADGKIMLAYTGNVKYPDGSRTAYQCLAQENAQGGYDKLGPVLPLPPGYSGHVRD
PKVWRHQDDWYMVLGARDLQDRGKVLLLRSADLHGWQLLGEIAGSQLNGIGDFGYMWECPDLFTLDGVEILICCPQGLAA
QAEQYLNVYQAGYFTGKLDYRQASFEHGAFHELDLGFEFYAPQTTQAEDGRRLLVGWMGVPEQDEDYHPTLQYGWIHILT
CPRELSLHQGKLYQQPARELQQLRGESRQWQGIADEAPCWPVGSAELLLTPEGAFSADFGGAMTLLWDGALLRLTRNNLR
NGQPEHRYWRGNLSQLQILFDRSSVEIFINHGEAVMSARYFPGPQPQLHLSGKTQLALEHWSLTPCMLE

Specific function: Enables the bacterium to metabolize sucrose as a sole carbon source [H]

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=386, Percent_Identity=25.9067357512953, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 52965; Mature: 52965

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEELNLMKQAVQALMRDMPKVLRAPHRPAWHLAPSVGLLNDPNGFIQHNGVYHLFYQWNP
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCEEECCEEEEEEEECC
LGCDHRNKCWGHWQSTDLVNWQHQPVALVPGACYDSHGCYSGSAVVADGKIMLAYTGNVK
CCCCCCCCCCCCCCCCCEECCCCCCEEEECCCCCCCCCCCCCCEEEECCEEEEEEECCEE
YPDGSRTAYQCLAQENAQGGYDKLGPVLPLPPGYSGHVRDPKVWRHQDDWYMVLGARDLQ
CCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCHHEEECCCEEEEEECCCCC
DRGKVLLLRSADLHGWQLLGEIAGSQLNGIGDFGYMWECPDLFTLDGVEILICCPQGLAA
CCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCEEECCCEEEECCEEEEEECCCCHHH
QAEQYLNVYQAGYFTGKLDYRQASFEHGAFHELDLGFEFYAPQTTQAEDGRRLLVGWMGV
HHHHHHHHHHCCEEECEECHHHCCCCCCCEEEECCCEEEECCCCCCCCCCCEEEEEECCC
PEQDEDYHPTLQYGWIHILTCPRELSLHQGKLYQQPARELQQLRGESRQWQGIADEAPCW
CCCCCCCCCCEEECEEEEEECCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PVGSAELLLTPEGAFSADFGGAMTLLWDGALLRLTRNNLRNGQPEHRYWRGNLSQLQILF
CCCCCEEEEECCCCCCCCCCCEEEEEECCEEEEEEHHHHCCCCCCCCEECCCCEEEEEEE
DRSSVEIFINHGEAVMSARYFPGPQPQLHLSGKTQLALEHWSLTPCMLE
ECCCEEEEEECCCEEEEEECCCCCCCEEEECCCEEEEEEECCCCCEECC
>Mature Secondary Structure
MEELNLMKQAVQALMRDMPKVLRAPHRPAWHLAPSVGLLNDPNGFIQHNGVYHLFYQWNP
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCEEECCEEEEEEEECC
LGCDHRNKCWGHWQSTDLVNWQHQPVALVPGACYDSHGCYSGSAVVADGKIMLAYTGNVK
CCCCCCCCCCCCCCCCCEECCCCCCEEEECCCCCCCCCCCCCCEEEECCEEEEEEECCEE
YPDGSRTAYQCLAQENAQGGYDKLGPVLPLPPGYSGHVRDPKVWRHQDDWYMVLGARDLQ
CCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCHHEEECCCEEEEEECCCCC
DRGKVLLLRSADLHGWQLLGEIAGSQLNGIGDFGYMWECPDLFTLDGVEILICCPQGLAA
CCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCEEECCCEEEECCEEEEEECCCCHHH
QAEQYLNVYQAGYFTGKLDYRQASFEHGAFHELDLGFEFYAPQTTQAEDGRRLLVGWMGV
HHHHHHHHHHCCEEECEECHHHCCCCCCCEEEECCCEEEECCCCCCCCCCCEEEEEECCC
PEQDEDYHPTLQYGWIHILTCPRELSLHQGKLYQQPARELQQLRGESRQWQGIADEAPCW
CCCCCCCCCCEEECEEEEEECCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PVGSAELLLTPEGAFSADFGGAMTLLWDGALLRLTRNNLRNGQPEHRYWRGNLSQLQILF
CCCCCEEEEECCCCCCCCCCCEEEEEECCEEEEEEHHHHCCCCCCCCEECCCCEEEEEEE
DRSSVEIFINHGEAVMSARYFPGPQPQLHLSGKTQLALEHWSLTPCMLE
ECCCEEEEEECCCEEEEEECCCCCCCEEEECCCEEEEEEECCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412665; 8628219 [H]